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Editing of eIF(iso)4E.c confers resistance against Turnip mosaic virus in Brassica rapa

Horticultural plant Journal 2024年第4期10卷 1020-1034页

作者： Yujia Liu Xiaoyun Xin Peirong Li Weihong Wang Yangjun Yu Xiuyun Zhao Deshuang Zhang Jiao Wang Fenglan Zhang Shujiang Zhang Shuancang Yu Tongbing Su State Key Laboratory of Vegetable Biobreeding Beijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China National Engineering Research center for Vegetables Beijing Key Laboratory of Vegetable Germplasms ImprovementKey Laboratory of Biology and Genetics Improvement of Horticultural Crops(North China)Key Laboratory of Urban Agriculture(North)of Ministry of AgricultureBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry ScienceBeijing 100097China Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China

Turnip mosaic virus(TuMV)constitutes one of the primary diseases affecting Brassica rapa,severely impacting its production and resulting in crop failures in various regions *** research has demonstrated the significance of plant translation initiation factors,specifically the eIF4E and eIF4G family genes,as essential recessive disease resistance *** our study,we conducted evolutionary and gene expression studies,leading us to identify e IF(iso)4E.c as a potential TuMV-resistant *** CRISPR/Cas9 technology,we obtained mutant *** plants with edited eIF(iso)4E.c *** confirmed eIF(iso)4E.c confers resistance against TuMV through phenotypic observations and virus content ***,we employed ribosome profiling assays on eif(iso)4e.c mutant seedlings to unravel the translation landscape in response to ***,we observed a moderate correlation between the fold changes in gene expression at the transcriptional and translational levels(R^(2)=0.729).Comparative analysis of ribosome profiling and RNA-seq data revealed that plant-pathogen interaction,and MAPK signaling pathway-plant pathways were involved in eIF(iso)4E.c-mediated TuMV *** analysis revealed that sequence features,coding sequence length,and normalized minimal free energy,influenced the translation efficiency of *** study highlights that the loss of e IF(iso)4E.c can result in a highly intricate translation mechanism,acting synergistically with transcription to confer resistance against TuMV.

关键词： Brassica rapa eIF(iso)4E.c TuMV Ribo-seq

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Glomerular organization in the antennal lobe of the oriental armyworm Mythimna separata

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Journal of Integrative Agriculture 2024年第11期23卷 3812-3829页

作者： Baiwei Ma Qi Chen Xi Chu Yidong Zuo Jiayu Wang Yi Yang Guirong Wang Bingzhong Ren Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization School of Life Sciences Northeast Normal University Key Laboratory of Vegetation Ecology Ministry of Education Northeast Normal University State Key Laboratory for biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Chemosensory Lab Department of Psychology Norwegian University of Science and Technology Shenzhen Branch Guangdong Laboratory for Lingnan Modern Agriculture Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences

In insects, the number and specificity of antennal lobe glomeruli often reflect the number and diversity of expressed chemosensory genes, which are linked to its ecological niche and specific olfactory needs. The oriental armyworm, Mythimna separata, is an important and common lepidopteran pest of cereal crops. Given its reliance on the olfactory system for crucial behaviors, understanding the evolutionary potential of this system requires a thorough characterization of the anatomical structure of the primary olfactory center. Here, we systematically identified all antennal lobe glomeruli of M. separata based on synaptic antibody immunostaining and mass staining of the olfactory sensory neurons. A total of 69 glomeruli were identified in females and 65 in males, and an intuitive nomenclature based on glomerular positions was applied. Our findings uncovered some sex-specific glomeruli in this species. There were ten female-specific glomeruli and three male-specific glomeruli, except for the macroglomerular complex(MGC) units, with a notable observation that the female labial pit organ glomerulus was larger than its male counterpart. Additionally, we identified four antennal-lobe tracts(ALTs) and retrograde labeling from the calyx revealed that all glomeruli were innervated by the medial ALT projection neurons. The comparison of the olfactory system structures between M. separata and sympatric moths supports their evolutionary convergence in noctuid moths. These results collectively lay the foundation for future studies on olfactory processing in M. separata.

关键词： antennal lobe glomerulus olfactory sensory neuron projection neuron antennal-lobe tract Mythimna separata

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plant photosystem Ⅱ assembly:TROL2 to the rescue

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Molecular plant 2023年第11期16卷 1719-1721页

作者： Sujith Puthiyaveetil Steven D.McKenzie Department of Biochemistry and center for plant biology Purdue UniversityWest LatayetteIN 47907USA

In one of the most consequential biochemical reactions on earth,a pigment-protein complex known as photosystem Ⅱ(PSⅡ)in photosynthesis extracts electrons,protons,and oxygen from water using energy from ***Ⅱ thereby sustains life on earth by harnessing the potential of two nearly inexhaustible raw materials:sunlight and *** plant PSllis a membrane-embedded *** monomer consists of around 28 unique protein subunits,157 chlorophylls,44 carotenoids,2 pheophytins,a heme,a non-heme iron,two quinones,and a Mn_(4)CaO_(5) cluster(Su et al.,2017;Cao et al.,2018).Within each monomeric complex,20 protein subunits constitute a core(Figure 1A).

关键词： Figure earth thereby

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Identif ication of a hypersensitive response core peptide of HrpZ and its role in increasing grape downy mildew resistance

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Horticultural plant Journal 2024年第5期10卷 1112-1120页

作者： Zongbao Fan Xueqiang Guan Zhichang Zhang Yushuai Sun Fei Wang Huiru Chi Yuxin Yao State Key Laboratory of Crop biology Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai'anShandong 271018China Shandong Academy of Grape/Shandong Technology Innovation center of Wine Grape and Wine JinanShandong 250100China Zhichang Agricultural Science and Technology Development Co. Ltd.RizhaoShandong 276511China

Harpins play a key role in inducing disease resistance in crops,and identifying their core functional regions and establishing a system for their efficient expression would be very *** this study,large amounts of soluble fusion proteins of harpin HrpZ and its subpeptides were obtained via the optimized induction conditions(28℃ with 0.5 mmol·L^(-1) IPTG for 6 h)in Escherichia coli BL21(DE3).Hypersensitive response(HR)assays demonstrated that the C-terminal 66 aa of HrpZ(HrpZ_C_2_2)elicited a strong HR in tobacco(Nicotiana benthamiana)and grape(Flame Seedless)***,treatment with HrpZ,and particularly HrpZ_C_2_2,significantly reduced the disease incidence and severity index of field vine leaves and those inoculated with downy *** determination of the physiological parameters indicated that HrpZ,and especially HrpZ_C_2_2,improved the photosynthesis-and chlorophyll fluorescence-related parameters,enhanced the activity of defense-related enzymes,including SOD,POD,CAT and PAL,and increased the H_(2)O_(2) ***,we efficiently expressed a core peptide of HrpZ and elucidated its strong ability to elicit a HR and resistance to downy *** research provides insight into understanding the structure and function of HrpZ and will advance the application of HrpZ_C_2_2 to increase the resistance of grapevine to downy mildew.

关键词： Grapevine Harpin HrpZ Prokaryotic expression Downy mildew Hypersensitive response

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Suppression of host plant defense by bacterial small RNAs packaged in outer membrane vesicles

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plant Communications 2024年第4期5卷 252-254页

作者： Yan Wu Sai Wang Peihong Wang Wenhan Nie Iftikhar Ahmad Panagiotis F.Sarris Gongyou Chen Bo Zhu Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station Shanghai Cooperative Innovation Center for Modern Seed Industry School of Agriculture and Biology Shanghai Jiao Tong University Shanghai China Department of Environmental Sciences COMSATS University Islamabad Vehari-Campus Vehari 61100 Pakistan Department of biology University of Crete 714 09 Heraklion Crete Greece Shanghai Jiao Tong University Chongqing Research Institute Shanghai China

(plant Communications 5,100817;April 82024)After a careful,in-depth examination,the authors found some additional errors in the published *** revision,the authors created a mutant DXosr001(in which the full-length Xosr001 was deleted)and a mutant DXosr001’(in which the predicted binding motif of Xosr001 was deleted)as shown in Figure S4 of the original *** RNA-FISH assays,Xosr001 transcript could not be detected in leaves inoculated with DXosr001-OMVs,while leaves inoculated with WT-and DXosr001’-OMVs could emit green fluorescence.

关键词： deleted Figure outer

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OsCPK12 phosphorylates OsCATA and OsCATC to regulate H2O2 homeostasis and improve oxidative stress tolerance in rice

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plant Communications 2024年第3期5卷 179-196页

作者： Beifang Wang Pao Xue Yingxin Zhang Xiaodeng Zhan Weixun Wu Ping Yu Daibo Chen Junlin Fu Yongbo Hong Xihong Shen Lianping Sun Shihua Cheng Qunen Liu Liyong Cao State Key Laboratory of Rice biology and Breeding China National Rice Research InstituteHangzhou 311400China Northern Rice Research center of Bao Qing Shuangyashan 155600China Zhejiang Key Laboratory of Super Rice Research China National Rice Research InstituteHangzhou 311400China

Calcium-dependent protein kinases(CPKs),the best-characterized calcium sensors in plants,regulate many aspects of plant growth and development as well as plant adaptation to biotic and abiotic ***,how CPKs regulate the antioxidant defense system remains largely *** previously found that impaired function of OsCPK12 leads to oxidative stress in rice,with more H2O2,lower catalase(CAT)activity,and lower ***,we explored the roles of OsCPK12 in oxidative stress tolerance in *** results show that OsCPK12 interacts with and phosphorylates OsCATA and OsCATC at *** of either OsCATA or OsCATC leads to an oxidative stress phenotype accompanied by higher accumulation of *** of the phosphomimetic proteins OsCATAS11D and OsCATCS11D in oscpk12-cr reduced the level of H2O2 ***,OsCATAS11D and OsCATCS11D showed enhanced catalase activity in vivo and in ***12-overexpressing plants exhibited higher CAT activity as well as higher tolerance to oxidative *** demonstrate that OsCPK12 affects CAT enzyme activity by phosphorylating OsCATA and OsCATC at Ser11 to regulate H2O2 homeostasis,thereby mediating oxidative stress tolerance in rice.

关键词： OsCPK12 OsCATs oxidative tolerance Oryza sativa L

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Five quinolizidine alkaloids with anti-tobacco mosaic virus activities from two species of Sophora

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Chinese Chemical Letters 2024年第6期35卷 380-384页

作者： Ji Zhang Tong Zhang Qiao An Peng Zhang Cai-Yan Tian Chun-Mao Yuan Ping Yi Zhan-Xing Hu Xiao-Jiang Hao State Key Laboratory of Functions and Applications of Medicinal plants Guizhou Medical UniversityGuiyang 550014China Natural Products Research center of Guizhou Province Guiyang 550014China School of Pharmaceutical Sciences Guizhou Medical UniversityGuiyang 550025China School of Pharmacy Guizhou University of Traditional Chinese MedicineGuiyang 550025China State Key Laboratory of Phytochemistry and plant Resources in West China Kunming Institute of BotanyChinese Academy of ScienceKunming 650201China Research Unit of Chemical biology of Natural Anti-Virus Products Chinese Academy of Medical SciencesKunming 650201China

Three novel matrine-type alkaloids(1-3)and two unprecedented aloperine-type alkaloids(4 and 5)were isolated from the root of Sophora tonkinensis and the seeds of Sophora alopecuroides ***,compound 1 possessed an unprecedented 6/5/6 tricyclic skeleton,while compounds 2 and 3 characterized by rare 6/6/5/6 tetracyclic system and 6/6/6/6/6 pentacyclic system ***,compound 4 possessed an unprecedented 6/7/6/6 tetracyclic core,and compound 5 characterized by rare 6/6/6/6tetracyclic *** structures were elucidated by comprehensive spectroscopic data analysis and electronic circular dichroism(ECD)*** tests indicated that compound 5 displayed significant anti-tobacco mosaic virus(TMV)activity compared with the positive control ningnanmycin.

关键词： Matrine-type alkaloids Aloperine-type alkaloids Sophora tonkinensis Sophora alopecuroides Anti-tobacco mosaic virus

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Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

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Journal of Integrative plant biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan Liu Yan Bai Zhisong Xu Hainan Zhao Haiming Zhao Jinsheng Lai Ya Liu Weibin Song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

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Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress

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Journal of Integrative Agriculture 2024年第10期23卷 3370-3386页

作者： Caixiang Wang Meili Li Dingguo Zhang Xueli Zhang Juanjuan Liu Junji Su State Key Laboratory of Aridland Crop Science/College of Life Science and Technology Gansu Agricultural UniversityLanzhou730070China Western Agricultural Research center Chinese Academy of Agricultural SciencesChangji 831100China College of biology and Geographical Sciences Yili Normal UniversityYining 835000China

Activity of bc1 complex kinase(ABC1K)is an atypical protein kinase(aPK)that plays a crucial role in plant mitochondrial and plastid stress responses,but little is known about the responses of ABC1Ks to stress in cotton(Gossypium spp.).Here,we identified 40 ABC1Ks in upland cotton(Gossypium hirsutum L.)and found that the Gh ABC1Ks were unevenly distributed across 17 *** GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental *** GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress *** qRT-PCR results revealed that most Gh ABC1Ks were upregulated by exposure to different *** ABC1K2-A05 and Gh ABC1K12-A07 expression levels were upregulated by at least three stress *** genes were further functionally characterized by virus-induced gene silencing(VIGS).Compared with the controls,the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde(MDA)contents,lower catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG *** addition,the expression levels of six stress marker genes(Gh DREB2A,Gh SOS1,Gh CIPK6,Gh SOS2,Gh WRKY33,and Gh RD29A)were significantly downregulated after stress in the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced *** results indicate that knockdown of Gh ABC1K2-A05 and Gh ABC1K12-A07 make cotton more sensitive to salt and PEG *** findings can provide valuable information for intensive studies of Gh ABC1Ks in the responses and resistance of cotton to abiotic stresses.

关键词： cotton ABC1K abiotic stress responses expression patterns virus-induced gene silencing(VIGS)

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Studies of the Variability of Primary Metabolites in the Epicarp, Mesocarp and Seed of Dacryodes edulis Fruit at Taste Maturity

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Advances in Biological Chemistry 2024年第2期14卷 64-72页

作者： Etou Ossibi Grace Jokael Mbon Nguékou Chrichina Ongouya Mouekouba Dalcantara Liana Mpika Joseph Attibayeba biology center Faculty of Applied Sciences (FSA) D&#233nis Sassou N&#8217guesso University Kint&#233l&#233 Congo Laboratory Biotechnology and plant Production Faculty of Science and Technology Marien Ngouabi University Brazzaville Congo

The fruits of Dacryodes edulis are rich in biologically active substances, which makes them of great interest in terms of validation. In this study, we targeted the primary metabolites in the epicarp, the mesocarp and the seed of the fruit of Dacryodes edulis at taste maturity, which were selected for their nutritional quality and its appreciation throughout the Gulf of Guinea area, which is very popular because of its large size, texture and special taste. The evaluation of total carbohydrates, total lipids and soluble proteins in the epicarp, mesocarp and seed of the fruit at taste maturity was made from spectrophotometer measurements. The overall analysis of the results of the present study shows that total carbohydrates, total lipids and proteins accumulate more in the seed with respectively 251.33 ± 1.15 mg/g DM;9.92 ± 0.201 mg/g DM and 55.075 ± 0.024 mg/g DM. Likewise, the results indicate low concentrations of total carbohydrates and total lipids in the epicarp with respectively 245 ± 1 mg/g DM and 4.77 ± 0.047 mg/g DM, on the other hand, it is the mesocarp which presents the lowest content of soluble proteins: 28.075 ± 3.231 mg/g DM. This variation could be linked to the nature of the compartment, more particularly to the storage location. This comparative study could lead to the valorization of the seed of the fruit of Dacryodes edulis for its richness in metabolites and arouse significant interest in nutrition.

关键词： Dacryodes edulis Fruit Taste Maturity Epicarp Mesocarp Seed

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