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Identification of an S5^n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

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Chinese Science Bulletin 2010年第13期55卷 1255-1262页

作者： SHAHID Muhammad Qasim provincial key laboratory of plant Molecular Breeding South China Agricultural University

Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S5n, revealing that 18 accessions carried the S5n allele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan province and 1 from Jiangxi province. Further sequence analysis spanning both sides of the deletion in S5n allele of O. rufipogon confirmed that this allele is identical with that of 02428, a wide-compatibility variety of domesticated rice. The segregation ratio of the 3 genotypes (S5iS5i/S5jS5j:S5nS5i/j:S5nS5n) among 15 self-bred progenies of O. rufipogon significantly deviated from the Mendelian expectation (1:2:1) in that the proportion of plants exhibiting S5n heterozygous and homozygous genotypes was much lower in the progeny. Sequence analysis of full-length S5n alleles indicated that a few base pairs were different between O. rufipogon and O. sativa, and it was presumed that S5n may be an ancient allele. The fertility of the embryo sac in plants with an S5n heterozygous genotype was low, suggesting the existence of other genes controlling embryo sac fertility in the wild rice.

关键词：普通野生稻等位基因胚囊广西壮族自治区鉴定序列分析遗传多样性广亲和品种

Graphene-based electrocatalysts for advanced energy conversion

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Green Energy & Environment 2023年第5期8卷 1265-1278页

作者： Pingping Yang Xin Yang Wenzhu Liu Ruike Guo Zufu Yao key laboratory of Research and Utilization of Ethnomedicinal plant Resources of Hunan province Hunan Engineering Laboratory for Preparation Technology of Polyvinyl Alcohol Fiber MaterialHuaihua UniversityHuaihua418000China Hunan provincial key laboratory of Dong Medicine Hunan University of MedicineHuaihua418000China

Graphene-based nanocatalysts have appealed much interest as advanced electrocatalysts toward energy conversion reactions due to their outstanding electrocatalytic performance from the distinctive chemical composites and strong synergistic *** to better understand the role of graphene played in enhancing the catalytic performance and offer guidance for fabricating more efficient graphenebased electrocatalysts,we herein summarize the remarkable achievements of graphene-based electrocatalysts for energy-conversion-related *** by discussing applications of graphene in the electrocatalytic reactions,we have manifested the crucial role of graphene played in promoting the catalytic ***,many representative graphene-based catalyst hybrids for electrocatalytic reactions are also overviewed,showing many effective strategies for the fabrication of more efficient graphene-related materials for the practical ***,the perspective insights and challenging issues are also concluded to provide directions for the future development.

关键词： Graphene Electrocatalysis Energy conversion Activity

Short-term transcriptomic responses of Populus euphratica roots and leaves to drought stress

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Journal of Forestry Research 2021年第2期32卷 841-853页

作者： Peipei Jiao Zhihua Wu Xu Wang Zhenbo Jiang Yanqin Wang Hong Liu Rui Qin Zhijun Li College of Life Sciences Tarim UniversityAlar 843300Xinjiang ProvincePeople’s Republic of China key laboratory of Biological Resource Protection and Utilization of Tarim Basin Xinjiang Production and Construction GroupAlar 843300Xinjiang ProvincePeople’s Republic of China hubei provincial key laboratory for Protection and Application of Special plant Germplasm in Wuling Area of China&key laboratory of State Ethnic Affairs Commission for Biological Technology College of Life SciencesSouth-Central University for NationalitiesWuhan 430074Hubei ProvincePeople’s Republic of China

Populus euphratica Oliv.,the Euphrates poplar,is the tallest tree species in the arid desert areas of Northwest *** of its drought-resistance genes is valuable to increase understanding of drought resistance ***-seq of leaves and roots under drought simulation by 25%polyethylene glycol-6000(PEG 6000)were performed at 0,4,and 12 *** and roots responded differently to drought via differentially upregulated and downregulated genes;more genes were downregulated than upregulated in both leaves and ***,these differentially expressed genes were enriched in different GO terms and KEGG *** example,GO term‘response to organic substance’and KEGG pathway‘nitrogen metabolism’were enriched for drought-stressed leaves,while GO term‘cell wall organization or biogenesis’and KEGG pathway‘zeatin biosynthesis’were enriched for drought-stressed *** enrichment of the GO term‘enzyme linked receptor protein signalling pathway’in both leaf and root drought responses suggests that these tissues may also have similar ***,roots under drought stress for four hs responded by activating programed cell *** KEGG pathway‘plant hormone signal transduction’was detected for 4-and 12-h droughtstressed leaves and 12-h drought-stressed roots,suggesting that plant hormone signal transduction plays an important role in both roots and *** enrichment of upregulated and downregulated genes for leaves and roots reflect differentially regulatory mechanisms of response to drought stress via different biological processes such as the regulation of photosynthesis and auxin signalling pathway in leaves,and the regulation of defence response and water homeostasis in *** candidate genes,including transcription factors,protein kinase,transporter,late embryogenesis abundant protein and mannitol dehydrogenase,were further selected to determine their response to drought using *** results show that the expression patte

关键词： Drought stress RNA-seq Populus euphratica qRT-PCR

Study on the Relationship Betweenthe Toxin of Phytophthora infestans(Mont.) de Bary and Resistance of Potato

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Agricultural Sciences in China 2011年第2期10卷 238-245页

作者： YANG Yan-li XIAO Lang-tao HU Xian-qi key laboratory of plant Pathaology of Yunnan province Yunnan Agricultural UniversityKunming 650201P.R.China Hunan provincial key laboratory of Phytohormones and Development Hunan Agricultural UniversityChangsha 410128P.R.China

plant pathogenic fungi produce mycotoxins which may lead host plants to produce disease symptoms and may have a significant positive role in the course of disease *** putative Phytophthora infestans toxins were prepared by culturing in liquid medium（60 g rye and 10% tomato juice L-1） for 1 mon at 17℃,filtering through four layers of cheesecloth and being precipitated by ammonium *** resulted putative toxin solutions were used to study the effects on 3 potato varieties（both leaf and tuber tissues）.The results show that potato leaves and tubers exhibit symptoms similar to the late blight resulted from *** *** varieties reacting differentially to both the toxin dilutions and toxins produced by different *** isolates suggested the presence of toxin-mediated specificities between potato and *** leaf and tuber tissues have different and contrary reactions to the ***-mediated specificities are likely present between potato and ***.

关键词： Phytophthora infestans toxin potato variety resistance

Calcium-dependent activation of CPK12 facilitates its cytoplasm-to-nucleus translocation to potentiate plant hypoxia sensing by phosphorylating ERF-Ⅶ transcription factors

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Molecular plant 2023年第6期16卷 979-998页

作者： Biao Fan Ke Liao Lin-Na Wang Li-Li Shi Yi Zhang Ling-Jing Xu Ying Zhou Jian-Feng Li Yue-Qin Chen Qin-Fang Chen Shi Xiao State key laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resourceschool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

Calcium-dependent protein kinases(CDPKs/CPKs)are key regulators of plant stress signaling that translate calcium signals into cellular responses by phosphorylating diverse substrate ***,the molecular mechanism by which plant cells relay calcium signals in response to hypoxia remains ***,we show that one member of the CDPK family in Arabidopsis thaliana,CPK12,is rapidly activated during hypoxia through calcium-dependent phosphorylation of its Ser-186 *** CPK12 shuttles from the cytoplasm to the nucleus,where it interacts with and phosphorylates the group Ⅶ ethylene-responsive transcription factors(ERF-Ⅶ)that are core regulators of plant hypoxia sensing,to enhance their ***,CPK12 knockdown lines show attenuated tolerance of hypoxia,whereas transgenic plants overexpressing CPK12 display improved hypoxia ***,loss of function of five ERF-Ⅶ proteins in an erf-vii pentuple mutant could partially suppress the enhanced hypoxia-tolerance phenotype of CPK12-overexpressing ***,we also discovered that phosphatidic acid and 14-3-3κ protein serve as positive and negative modulators of the CPK12 cytoplasm-to-nucleus translocation,*** together,these findings uncover a CPK12-ERF-Ⅶ regulatory module that is key to transducing calcium signals from the cytoplasm into the nucleus to potentiate hypoxia sensing in plants.

关键词： CPK12 hypoxia sensing cytoplasm-to-nucleus translocation ERF-Ⅶtranscription factors phospha-tidic acid 14-3-3 proteins

A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis

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aBIOTECH 2024年第2期5卷 140-150页

作者： Feng-Zhu Wang Ying Bao Zhenxiang Li Xiangyu Xiong Jian-Feng Li State key laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

The CRISPR/Cas9 technology revolutionizes targeted gene knockout in diverse organisms including ***,screening edited alleles,particularly those with multiplex editing,from herbicide-or antibiotic-resistant transgenic plants and segregating out the Cas9 transgene represent two laborious *** solutions to facilitate these processes rely on different selection ***,by taking advantage of the opposite functions of a D-amino acid oxidase(DAO)in detoxifying D-serine and in metabolizing non-toxic D-valine to a cytotoxic product,we develop a DAO-based selection system that simultaneously enables the enrichment of multigene edited alleles and elimination of Cas9-containing progeny in Arabidopsis *** five DAOs tested in Escherichia coli,the one encoded by Trigonopsis variabilis(TvDAO)could confer slightly stronger D-serine resistance than other *** expression of TvDAO in Arabidopsis allowed a clear distinction between transgenic and nontransgenic plants in both D-serine-conditioned positive selection and D-valine-conditioned negative *** a proof of concept,we combined CRISPR-induced single-strand annealing repair of a dead TvDAO with D-serine-based positive selection to help identify transgenic plants with multiplex editing,where D-serine-resistant plants exhibited considerably higher co-editing frequencies at three endogenous target genes than those selected by ***,D-valine-based negative selection successfully removed Cas9 and TvDAO transgenes from the survival offspring carrying inherited ***,this work provides a novel strategy to ease CRISPR mutant identification and Cas9 transgene elimination using a single selection marker,which promises more efficient and simplified multiplex CRISPR editing in plants.

关键词： CRISPR Genome editing D-amino acid oxidase Cas9-free Selection marker

Pathogen-informed breeding for crop disease resistance

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Journal of Integrative plant Biology 2021年第2期63卷 305-311页

作者： Qi Li Bi Wang Jinping Yu Daolong Dou Jiangsu key laboratory for the Research and Utilization of plant Resources Institute of BotanyJiangsu Province and Chinese Academy of SciencesNanjing 210014China Department of plant pathology Nanjing Agricultural UniversityNanjing 210095China

The development of durable and broad-spectrum resistance is an economical and eco-friendly approach to control crop diseases for sustainable agricultural production. Emerging knowledge of the molecular basis of pathogenesis and plant–pathogen interactions has contributed to the development of novel pathogen-informed breeding strategies beyond the limits imposed by conventional breeding. Here,we review the current status of pathogen-assisted resistance-related gene cloning. We also describe how pathogen effector proteins can be used to identify resistance resources and to inform cultivar deployment. Finally, we summarize the main approaches for pathogen-directed plant improvement,including transgenesis and genome editing. Thus, we describe the emerging role of pathogen-related studies in the breeding of disease-resistant varieties, and propose innovative pathogen-informed strategies for future applications.

关键词： disease resistance breeding effector protein plant immune system plant pathogens

Autophagy in plants: Physiological roles and post-translational regulation

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Journal of Integrative plant Biology 2021年第1期63卷 161-179页

作者： Hua Qi Fan-Nv Xia Shi Xiao State key laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

In eukaryotes, autophagy helps maintain cellular homeostasis by degrading and recycling cytoplasmic materials via a tightly regulated *** the past few decades, significant progress has been made towards understanding the physiological functions and molecular regulation of autophagy in plant cells. Increasing evidence indicates that autophagy is essential for plant responses to several developmental and environmental cues, functioning in diverse processes such as senescence, male fertility, root meristem maintenance, responses to nutrient starvation,and biotic and abiotic stress. Recent studies have demonstrated that, similar to nonplant systems,the modulation of core proteins in the plant autophagy machinery by posttranslational modifications such as phosphorylation, ubiquitination,lipidation, S-sulfhydration, S-nitrosylation, and acetylation is widely involved in the initiation and progression of autophagy. Here, we provide an overview of the physiological roles and posttranslational regulation of autophagy in plants.

关键词： autophagy physiological roles post-translational regulation

The MYB transcription factor OsMYBxoc1 regulates resistance to Xoc by directly repressing transcription of the iron transport gene OsNRAMP5 in rice

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plant Communications 2024年第6期5卷 230-245页

作者： Haimiao Zhang Baolong Sun Wei Wu Yang Li Ziyi Yin Chongchong Lu Haipeng Zhao Lingguang Kong Xinhua Ding State key laboratory of Crop Biology Shandong Provincial Key Laboratory of Agricultural MicrobiologyCollege of Plant ProtectionShandong Agricultural UniversityTai’an 271018China

Bacterial leaf streak caused by Xanthomonas oryzae ***(Xoc)is a continuous threat to rice cultivation,leading to substantial yield losses with socioeconomic *** ions are essential mineral nutrients for plant growth,but little information is available on how they influence mechanisms of rice immunity against ***,we investigated the role of the myeloblastosis-related(MYB)transcriptional repressor OsMYBxoc1 in modulation of rice resistance through control of iron ion *** of OsMYBxoc1 significantly increased rice resistance,whereas OsMYBxoc1 RNA-interference lines and knockout mutants showed the opposite *** of OsMYBxoc1 expression dampened the immune response induced by pathogen-associated molecular *** demonstrated that OsMYBxoc1 binds specifically to the OsNRAMP5 promoter and represses transcription of ***5,a negative regulator of rice resistance to bacterial leaf streak,possesses metal ion transport activity,and inhibition of OsMYBxoc1 expression increased the iron ion content in *** of the iondependent H2O2 scavenging enzyme catalase was increased in plants with suppressed expression of OsMYBxoc1 or overexpression of *** found that iron ions promoted Xoc infection and interfered with the production of reactive oxygen species induced by *** type Ⅲ effector XopAK directly inhibited OsMYBxoc1 transcription,indicating that the pathogen may promote its own proliferation by relieving restriction of iron ion transport in *** addition,iron complemented the pathogenicity defects of the RS105_DXopAK mutant strain,further confirming that iron utilization by Xoc may be dependent upon *** conclusion,our study reveals a novel mechanism by which OsMYBxoc1 modulates rice resistance by regulating iron accumulation and demonstrates that Xoc can accumulate iron ions by secreting the effector XopAK to promote its own infection.

关键词： pathogen effector plant immunity bacterial leaf streak metal ion