Calcium-dependent activation of CPK12 facilitates its cytoplasm-to-nucleus translocation to potentiate plant hypoxia sensing by phosphorylating ERF-Ⅶ transcription factors
作者机构：State Key Laboratory of BiocontrolGuangdong Provincial Key Laboratory of Plant Resourceschool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第6期
页 面：979-998页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by the National Natural Science Foundation of China(Projects 31725004,U22A20458) the Key Realm Research and Development Program of Guangdong Province(Project 2020B0202090001) the Natural Science Foundation of Guangdong Province(Project 2023A1515012038)
主 题：CPK12 hypoxia sensing cytoplasm-to-nucleus translocation ERF-Ⅶtranscription factors phospha-tidic acid 14-3-3 proteins
摘 要：Calcium-dependent protein kinases(CDPKs/CPKs)are key regulators of plant stress signaling that translate calcium signals into cellular responses by phosphorylating diverse substrate ***,the molecular mechanism by which plant cells relay calcium signals in response to hypoxia remains ***,we show that one member of the CDPK family in Arabidopsis thaliana,CPK12,is rapidly activated during hypoxia through calcium-dependent phosphorylation of its Ser-186 *** CPK12 shuttles from the cytoplasm to the nucleus,where it interacts with and phosphorylates the group Ⅶ ethylene-responsive transcription factors(ERF-Ⅶ)that are core regulators of plant hypoxia sensing,to enhance their ***,CPK12 knockdown lines show attenuated tolerance of hypoxia,whereas transgenic plants overexpressing CPK12 display improved hypoxia ***,loss of function of five ERF-Ⅶ proteins in an erf-vii pentuple mutant could partially suppress the enhanced hypoxia-tolerance phenotype of CPK12-overexpressing ***,we also discovered that phosphatidic acid and 14-3-3κ protein serve as positive and negative modulators of the CPK12 cytoplasm-to-nucleus translocation,*** together,these findings uncover a CPK12-ERF-Ⅶ regulatory module that is key to transducing calcium signals from the cytoplasm into the nucleus to potentiate hypoxia sensing in plants.
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