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Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking

World Journal of Gastroenterology 2006年第36期12卷 5846-5852页

作者： Soichi Kitano Hisashi Hisatomi Nozomu Hibi Katsumi Kawano Shoji Harada Technology Development Department SRL Inc. Hachioji Tokyo Japan Center for Molecular Biology and Cytogenetics SRL Inc. Hino Tokyo Japan

AIM: To develop a simple and accurate method for quantifying 8-isoprostane in plasma by employing a combination of two-step solid-phase extraction of samples and a commercially available ELISA kit, and by this method to examine the effects of drinking and smoking habits against the levels of plasma 8-isoprostane in healthy Japanese ***: Plasma 8-isoprostane was extracted with ODS gel suspension followed by NH2 Sep-Pak column. The 8-isoprostane fractions were assayed using a commercially available ELISA kit. We measured plasma 8-isoprostane levels in 157 healthy Japanese volunteers divided into three groups (64 non-habitual drinkers, 56 moderate drinkers and 37 habitual drinkers) according to their alcohol consumption per week. Genotypes of aldehyde dehydrogenase 2 (ALDH2) were also determined to investigate the plasma 8-isoprostane levels with reference to drinking habits. In addition, the plasma 8-isoprostane levels of 96 non-smokers and 61 smokers from the same subjects were ***: Our method fulfilled all the requirements for use in routine clinical assays with respect to sensitivity, intra- and inter-assay reproducibility, accuracy and dynamic assay range. Significant increases of plasma 8-isoprostane levels were observed in female habitual drinkers when compared with those of non-habitual drinkers (t = 5.494, P < 0.0001) as well as moderate drinkers (t = 3.542, P < 0.005), and 8-isoprostane levels were also significantly different between ALDH2*2/1 and ALDH2*1/1 in the female habitual drinkers (t = 6.930, P < 0.0001), suggesting that excessive drinking of alcohol may increase oxidization stress, especially in females. On the contrary, no significant difference of the plasma 8-isoprostane levels was observed between non-smokers and ***: Our present method was proved to be a simple and accurate tool for measuring plasma 8-isoprostane. However, the clinical utility of plasma 8-isoprostane for drinking and smoking habits was

关键词： 8-Isoprostane ELISA Lipid peroxidation Drinking Smoking

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The new knowledge is written on sedimentary rocks——a comment on Shanmugam’s paper “the hyperpycnite problem”

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Journal of Palaeogeography 2019年第3期8卷 306-313页

作者： Carlos Zavala Departamento de Geología Universidad Nacional del Sur GCS Argentina srl

In a recent contribution G. Shanmugam (2018) discusses and neglects the importance of hyperpycnal flows and hyperpycnites for the understanding of some sediment gravity flow deposits. For him, the hyperpycnal flow paradigm is strictly based on experimental and theoretical concepts, without the supporting empirical data from modern depositional systems. In this discussion I will demonstrate that G. Shanmugam overlooks growing evidences that support the importance of hyperpycnal flows in the accumulation of a huge volume of fossil clastic sediments. Sustained hyperpycnal flows also provide a rational explanation for the origin of well sorted fine-grained massive sandstones with floating clasts, a deposit often wrongly related to sandy debris flows.

关键词： Hyperpycnites Turbidites Sediment gravity flows

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PoInTree: A Polar and Interactive Phylogenetic Tree

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Genomics, Proteomics & Bioinformatics 2005年第1期3卷 58-60页

作者： Carreras Marco Gianti Eleonora Sartori Luca Plyte Simon Edward Isacchi Antonella Bosotti Roberta Nerviano Medical Sciences srl 20014 Nerviano (MI) Italy.Nerviano Medical Sciences srl 20014 Nerviano (MI) Italy.Nerviano Medical Sciences srl 20014 Nerviano (MI) Italy.Nerviano Medical Sciences srl 20014 Nerviano (MI) Italy.Nerviano Medical Sciences srl 20014 Nerviano (MI) Italy.Nerviano Medical Sciences srl 20014 Nerviano (MI) Italy.

PoInTree （Polar and Interactive Tree） is an application that allows to build, visualize, and customize phylogenetic trees in a polar, interactive, and highly flexible view. It takes as input a FASTA file or multiple alignment formats. Phylogenetic tree calculation is based on a sequence distance method and utilizes the Neighbor Joining （N J） algorithm. It also allows displaying precalculated trees of the major protein families based on Pfam classification. In PoInTree, nodes can be dynamically opened and closed and distances between genes are graphically represented. Tree root can be centered on a selected leaf. Text search mechanism, color-coding and labeling display are integrated. The visualizer can be connected to an Oracle database containing information on sequences and other biological data, helping to guide their interpretation within a given protein family across multiple species. The application is written in Borland Delphi and based on VCL Teechart Pro 6 graphical component （Steema software）.

关键词： phylogenetic tree tree visualizer tree builder

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Assessment of sensory properties and in vitro antimicrobial activity of monofloral Sicilian honey

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Food Bioscience 2023年第2期52卷 791-801页

作者： Nunziatina Russo Ambra Rita Di Rosa Alessandra Pino Gaetana Mazzeo Luigi Liotta Cinzia Caggia Cinzia Lucia Randazzo Department of Agriculture Food and Environment(Di3A)University of CataniaVia Santa Sofia 10095123CataniaItaly probioetna srl Spin-off of University of CataniaVia S.Sofia10095123CataniaItaly Department of Veterinary Sciences University of MessinaPolo Universitario Annunziata98168MessinaItaly CERNUT(Interdepartmental Research Centre in Nutraceuticals and Health Products) University of CataniaVia le A.Doria 695125CataniaItaly

The antimicrobial and antioxidant properties of honey are well-established and recently the potential of honey for biotechnological and food preservative applications appeared *** to that,the present study evaluated the sensory profile and the antimicrobial potential of honey samples produced in different phytogeographical zones,in the Sicilian areas,and obtained from five different floral sources(chestnut,eucalyptus,sulla,thyme,and citrus).The specific sensory fingerprint of the analyzed samples was assessed by artificial senses(E-Eye,E-Nose,and E-Tongue).In addition,the antimicrobial activity against Staphylococcus aureus ATCC 29213,Listeria monocytogenes ATCC 19114,Bacillus subtilis DSM 10,Escherichia coli ATCC 25922,Enterococcus faecalis ATCC 29212,Pseudomonas aeruginosa ATCC 9027,Salmonella enterica Typhimurium ATCC 14026,and Candida albicans ATCC 10231 was *** senses allowed to confirm the impact of climate factors and genetic features on honey composition suggesting implications for antimicrobial activity.A broad spectrum of antagonistic activity was displayed by the analyzed honey samples in both undiluted and diluted *** particular,thyme honey showed interesting antagonistic *** addition,a greater inhibitory effect of amber-colored honey than light ones was observed.

关键词： Artificial senses Sensory properties Honeybees Antimicrobial potential Growth inhibition

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A scalable decentralized system for fair token distribution and seamless users onboarding

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Blockchain(Research and Applications) 2022年第1期3卷 40-48页

作者： Francesco Bruschi Manuel Tumiati Vincenzo Rana Mattia Bianchi Donatella Sciuto Politecnico di Milano Milano20133Italy KNOBS srl Milan20121Italy

Tokens are digital,transferable,and programmable assets and one of the most promising tools offered by *** could enable a wide range of applications,from down to earth to *** of the main issues in achieving wide adoption of tokens is onboarding:main platforms require users to deal with specific tools such as wallets,transaction fees,key generation,and *** most common solution to offer a familiar experience to naive users are custodial intermediaries,which have the important drawback of centralizing the process,and keeping users away from the advantages of selfsovereign assets *** this paper,we present a process for the distribution of digital tokens to end users,exploiting“physical”objects for initial *** process is aimed at making the onboarding of users not yet accustomed to blockchain tools and concepts as easy as possible,in a secure and decentralized way.

关键词： Blockchain Tokens Onboarding Wallets

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Proof of concept of a self-tightening needle-less suture using a NiTi shapememory alloy

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Bio-Design and Manufacturing 2023年第5期6卷 536-549页

作者： Adelaide Nespoli Davide Ninarello Enrico Bassani Villiam Dallolio Institute of Condensed Matter Chemistry and Technologies for Energy National Research Council(CNR-ICMATE)23900 LeccoItaly Promev srl 23900 LeccoItaly

Among surgical procedures,suturing is considered ***,in some situations,suturing is not easy or ***,we present proof of concept of a new self-tightening needle-less suture made of a NiTi *** new device is designed to overcome two demanding steps of traditional suturing:needle introduction and manual *** novel suture is composed only of a short NiTi wire that is able to change shape by exploiting a thermoelastic phase *** shape change is achieved by increasing the temperature from that of the operating room(e.g.,20-22℃)to 32℃using warm *** consists of two steps:suture introduction,in which the suture is in an open configuration,and shape recovery(i.e.,closed configuration).The closed configuration is maintained at human body temperature thanks to peculiar pseudoelastic properties of the NiTi ***,thermal and functional characterization verified the simplicity and effectiveness of the proposed device.

关键词： Suture NiTi Shape memoryalloys Suturing Medical device

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Polyethoxylation and polypropoxylation reactions：Kinetics,mass transfer and industrial reactor design

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Chinese Journal of Chemical Engineering 2018年第6期26卷 1235-1251页

作者： E.Santacesaria R.Tesser M.Di Serio Eurochem Engineering srl. Via Codogno 5 IT-20139 Milan Italy University of Naples 'Federico II' Department of Chemical Sciences Via Cinthia IT-80126 Naples Italy

Ethoxylation and propoxylation reactions are performed in the industry to produce mainly non-ionic surfactants and ethylene oxide(EO)–propylene oxide(PO) *** the reactions occur in gas–liquid reactors by feeding gaseous EO,PO or both into the reactor containing a solution of an alkaline catalyst(KOH or Na OH).Non-ionic surfactants are produced by using liquid starters like fatty alcohols,fatty acids or alkyl-phenols,while when the scope is to prepare EO–PO copolymers the starter can be a mono-or multi-functional alcohol of low molecular *** reactions are strongly exothermic,and EO and PO,in some conditions,can give place to runaway and also to explosive side ***,the choice of a suitable reactor is a key factor for operating in safe conditions.A correct reactor design requires:(i) the knowledge of the kinetic laws governing the rates of the occurring reactions;(ii) the role of mass and heat transfer in affecting the reaction rate;(iii) the solubility of EO and PO in the reacting mixture with the non-ideality of the reacting solutions considered;(iv) the density of the reacting *** these aspects have been studied by our research group for different starters of industrial interest,and the data collected by using semibatch well stirred laboratory reactors have been employed for the simulation of industrial reactors,in particular Gas–Liquid Spray Tower Loop Reactors.

关键词： Ethoxylation Propoxylation Kinetics Mass transfer Spray tower loop reactor

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Molecular Characterization of Peruvian Fowl Adenovirus (FAdV) Isolates

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Advances in Microbiology 2014年第10期4卷 595-603页

作者： Jorge Rodríguez Ysabel Koga Arnaldo Alvarado Robert Tinoco Bioservice srl Lima Peru

Forty seven clinical samples of Fowl adenovirus (FAdV) associated with Inclusion Body Hepatitis (IBH) from Peruvian broilers received between July 2006 and April 2013 were genotyped using sequencing of L1 Loop of Hexon gene. All 47 clinical samples presented macroscopic and histopathology lesions consistent with IBH, and amplified a specific fragment of Hexon gene by Polymerase Chain Reaction (PCR). A unique nucleotide sequence of 789 base pairs of Hexon gene (position 273 to 1061) was obtained in all 47 clinical samples analyzed. This sequence showed a high level of conservation in amino acid and nucleotide sequence (>99%) with a Fowl Adenovirus C serotype 4 previously identified. Sequence and phylogenetic analysis indicate no genotypic variation in Peruvian isolates. The presence of a unique genotype very closely related with genotype C1 previously reported in Peru and Ecuador (>99%), suggests the presence of FAdV C serotype 4 genotype C1 in clinical cases of IBH from Peruvian broilers.

关键词： Fowl Adenovirus C Molecular Characterization Inclusion Body Hepatitis Peru

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Functional lipidomics in patients on home parenteral nutrition: Effect of lipid emulsions

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World Journal of Gastroenterology 2017年第25期23卷 4604-4614页

作者： Loris Pironi Mariacristina Guidetti Ornella Verrastro Claudia Iacona Federica Agostini Caterina Pazzeschi Anna Simona Sasdelli Michele Melchiorre Carla Ferreri Chronic Intestinal Failure Center Digestive Disease DepartmentSt Orsola-Malpighi Hospital Lipinutragen srl-Lipidomics Laboratory ISOF National Council of Research

To investigate the fatty acid-based functional lipidomics of patients on long-term home parenteral nutrition receiving different intravenous lipid emulsions. METHODSA cross-sectional comparative study was carried out on 3 groups of adults on home parenteral nutrition (HPN), receiving an HPN admixture containing an olive-soybean oil-based intravenous lipid emulsion (IVLE) (OO-IVLE; n = 15), a soybean- medium-chain triacylglycerol-olive-fish oil-based IVLE (SMOF-IVLE; n = 8) or HPN without IVLE (No-IVLE; n = 8) and 42 healthy controls (HCs). The inclusion criteria were: duration of HPN ≥ 3 mo, current HPN admixtures ≥ 2 mo and HPN infusions ≥ 2/wk. Blood samples were drawn 4-6 h after the discontinuation of the overnight HPN infusion. The functional lipidomics panel included: the red blood cell (RBC) fatty acid (FA) profile, molecular biomarkers [membrane fluidity: saturated/monounsaturated FA ratio = saturated fatty acid (SFA)/monounsaturated fatty acid (MUFA) index; inflammatory risk: n-6/n-3 polyunsaturated fatty acid (PUFA) ratio = n-6/n-3 index; cardiovascular risk: sum of n-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) = n-3 index; free radical stress: sum of FA trans isomers = %trans index] and FA pathway enzyme activity estimate (delta-9-desaturase = D9D; delta-6-desaturase = D6D; delta-5-desaturase = D5D; elongase = ELO). Statistics were carried out using nonparametric tests. The amount of each FA was calculated as a percentage of the total FA content (relative%). RESULTSIn the OO-IVLE group, the percentage of oleic acid in the RBCs was positively correlated with the weekly load of OO-IVLE (r = 0.540, P = 0.043). In the SMOF-IVLE cohort, the RBC membrane EPA and DHA were positively correlated with the daily amount of SMOF-IVLE (r = 0.751, P = 0.044) and the number of HPN infusions per week (r = 0.753; P = 0.046), respectively. The SMOF-IVLE group showed the highest EPA and DHA and the lowest arachidonic acid percentages (P < 0.001). The RBC mem

关键词： Chronic intestinal failure Home parenteral nutrition Intravenous lipid emulsion Cell membrane fatty acid profile Cell membrane lipidome Functional lipidomics

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Seed-Specific Expression of Apolipoprotein A-IMilano Dimer in Engineered Rice Lines

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Rice science 2023年第6期30卷 587-597页

作者： Serena REGGI Elisabetta ONELLI Alessandra MOSCATELLI Nadia STROPPA Matteo DELL’ANNO Kiril PERFANOV Luciana ROSSI Department of Veterinary Medicine and Animal Science University of MilanLodi 26900Italy Department of Biosciences University of MilanMilan 20133Italy Plantechno srl Casalmaggiore 26040Italy

Apolipoprotein A-IMilano(ApoA-IM)has been shown to significantly reduce coronary atherosclerotic ***,the preparation of cost-effective pharmaceutical formulations of ApoA-IM is limited by the high cost and difficulty of purifying the protein and producing the highly effective dimeric *** aim of this study was to create an expression cassette that specifically drives the expression of dimeric ApoA-IM in the protein bodies of rice *** ApoA-IM protein under control of the 13 kDa prolamin promoter is expressed exclusively in its dimeric form within the seeds,and immunocytochemical and immunogold analyses confirmed its expression in different caryopsis tissue such as seed coat,aleurone cell and endosperm,particularly in amyloplast and storage vacuoles.A plant-based ApoA-IM production system offered numerous advantages over current production systems,including the direct production of the most therapeutically effective dimeric ApoA-IM forms,long-term protein storage in seeds,and ease of protein production by simply growing ***,seeds had the potential to serve as a costeffective source of therapeutic ApoA-IM.

关键词： apolipoprotein A-IMilano engineered plant immunofluorescence immunogold analysis rice seed-specific promoter

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