Seed-Specific Expression of Apolipoprotein A-IMilano Dimer in Engineered Rice Lines

作     者：Serena REGGI Elisabetta ONELLI Alessandra MOSCATELLI Nadia STROPPA Matteo DELL’ANNO Kiril PERFANOV Luciana ROSSI Serena REGGI;Elisabetta ONELLI;Alessandra MOSCATELLI;Nadia STROPPA;Matteo DELL'ANNO;Kiril PERFANOV;Luciana ROSSI

作者机构：Department of Veterinary Medicine and Animal ScienceUniversity of MilanLodi 26900Italy Department of BiosciencesUniversity of MilanMilan 20133Italy Plantechno SrlCasalmaggiore 26040Italy 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2023年第30卷第6期

页      面：587-597页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

主　　题：apolipoprotein A-IMilano engineered plant immunofluorescence immunogold analysis rice seed-specific promoter 

摘      要：Apolipoprotein A-IMilano(ApoA-IM)has been shown to significantly reduce coronary atherosclerotic ***,the preparation of cost-effective pharmaceutical formulations of ApoA-IM is limited by the high cost and difficulty of purifying the protein and producing the highly effective dimeric *** aim of this study was to create an expression cassette that specifically drives the expression of dimeric ApoA-IM in the protein bodies of rice *** ApoA-IM protein under control of the 13 kDa prolamin promoter is expressed exclusively in its dimeric form within the seeds,and immunocytochemical and immunogold analyses confirmed its expression in different caryopsis tissue such as seed coat,aleurone cell and endosperm,particularly in amyloplast and storage vacuoles.A plant-based ApoA-IM production system offered numerous advantages over current production systems,including the direct production of the most therapeutically effective dimeric ApoA-IM forms,long-term protein storage in seeds,and ease of protein production by simply growing ***,seeds had the potential to serve as a costeffective source of therapeutic ApoA-IM.