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Journal of Materials Science & Technology 2021年第19期78卷 92-99页

作者： Peng Wan Weidan Wang Lizhen Zheng Ling Qin Ke Yang School of Materials Science and Engineering Dongguan University of TechnologyDongguan523808China Li Ka Shing institute of Health Sciences Department of Orthopaedics and TraumatologyThe Chinese University of Hong KongHong KongChina Department of orthopaedics Affiliated Zhongshan Hospital of Dalian UniversityDalian116001China institute of Metal research Chinese Academy of SciencesShenyang110016China

Osteoporotic fracture with increase of aging population became an urgent orthopedic *** were widely recommended as effective clinical treatment *** of biodegradable Mg-based implants and merits of bisphosphonates was suggested for osteoporotic fracture *** the mild and sustained drug release,a novel one-step electrodeposition synthesis of drug loaded coating was proposed in this *** comparison to conventional soaking method,encapsulated zoledronate coating by one-step electrodeposition method could modulate drug release in first diffusion-controlled and later degradation-controlled *** in vitro cell response to zoledronate loaded coating showed enhanced proliferation and osteogenic differentiation of osteoblasts and no significant inhibition on osteoclasts,which could improve bone-forming and decrease bone resorption due to osteoporosis.

关键词： Bisphosphonate Magnesium Coating Electrodeposition Osteoporosis

A translational study of somatosensory evoked potential time–frequency components in rats,goats,and humans

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Neural Regeneration research 2021年第11期16卷 2269-2275页

作者： Hong-Yan Cui Yi-Xin Wu Rong Li Guang-Sheng Li Yong Hu institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjinChina Department of orthopaedics and traumatology The University of Hong KongHong Kong Special Administrative RegionChina Spinal Division Department of OrthopaedicsAffiliated Hospital of Guangdong Medical UniversityGuangdong ProvinceChina Department of orthopaedics and traumatology The University of Hong Kong-Shenzhen HospitalShenzhenGuangdong ProvinceChina

Somatosensory evoked potentials(SEPs)have been widely used to assess neurological function in clinical practice.A good understanding of the association between SEP signals and neurological function is helpful for precise diagnosis of impairment *** studies on SEPs have been reported in animal ***,few studies have reported the relationships between SEP waveforms in animals and those in *** this study,we collected normal SEP waveforms and decomposed them into specific time–frequency components(TFCs).Our results showed three stable TFC distribution regions in intact goats and rats and in *** we induced spinal cord injury in the animal models,a greater number of small TFC distribution regions were observed in the injured goat and rat groups than in the normal ***,there were significant correlations(Presearch of Li Ka Shing Faculty of Medicine of the University of Hong Kong(approval *** 2912-12)on January 28,*** experiments were approved by the Animal Ethics Committee of Affiliated Hospital of Guangdong Medical University(approval ***2002132)on March 5,2018.

关键词： animal models chronic compression latency somatosensory evoked potentials spinal cord injury time-frequency analysis time-frequency components translational study

3D bioprinting of cell-laden constructs for regenerative medicine

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Engineered Regeneration 2021年第1期2卷 195-205页

作者： Cuidi Li Wenguo Cui Department of orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

Human tissue consists of various tissue-specific cells,extracellular matrix components and microstructures,and growth *** promising multi-cell and multi-material integration manufacturing feature,3D extrusion bioprinting has shown outstanding application potential in the field of regenerative *** functional tissue regeneration,bioprinted constructs not only play the role of a cell-delivery system,but also serve as an important host niche for cell proliferation and *** order to meet the specific requirements of different tissue regeneration,development of bio-inks that provide tissue-specific biophysical cues and biochemical microenvironments is an important research ***,reconstruction of tissues with anisotropic structure,such as articular cartilage and meniscus,largely depend on the design of 3D bioprinting path for accurate arrangement of specific *** review summarizes the advanced designs of tissue-specific 3D bioprinting of cell-laden constructs for functional regeneration of skeletal and locomotor systems such as bone,cartilage,skeletal muscle,and blood vessels via the collaboration of bio-ink and printing *** may provide a basis for synergistic design for functional regenerative constructs bioprinting in the future.

关键词： 3D bioprinting Tissue regeneration Hydrogel Microenvironment

Efficacy of the new therapeutic approach in curing malignant neoplasms on the model of human glioblastoma

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Cancer Biology & Medicine 2021年第3期18卷 910-930页

作者： Evgeniya V.Dolgova Oleg M.Andrushkevich Polina E.Kisaretova Anastasia S.Proskurina Genrikh S.Ritter Tatyana D.Dubatolova Margarita V.Romanenko Oleg S.Taranov Yaroslav R.Efremov Evgeniy L.Zavyalov Alexandr V.Romaschenko Sergey V.Mishinov Svetlana S.Kirikovich Evgeniy V.Levites Ekaterina A.Potter Alexandr A.Ostanin Elena R.Chernykh Stanislav Yu.Roshchin Anatoliy V.Bervitskiy Galina I.Moysak Jamil A.Rzaev Sergey S.Bogachev institute of Cytology and Genetics SB RAS Novosibirsk 630090Russia A.I.Evdokimov moscow State University of Medicine and Dentistry Moscow 127473Russia institute of Molecular and Cellular Biology Novosibirsk 630090Russia Novosibirsk State University Novosibirsk 630090Russia The State research Center of Virology and Biotechnology"Vector" KoltsovoNovosibirsk 630559Russia First Department of Neurosurgery Ya.L.Tsivian Novosibirsk Research Institute of Traumatology and OrthopaedicsNovosibirsk 630091Russia institute of Fundamental and Clinical immunology Novosibirsk 630099Russia Sklifosovsky research institute of Emergency Medicine Moscow 129010Russia Federal Center of Neurosurgery Novosibirsk 630048Russia

Objective:Glioma is a highly invasive tumor,frequently disposed in essential areas of the brain,which makes its surgical excision extremely difficult;meanwhile adjuvant therapy remains quite ***:In the current report,a new therapeutic approach in curing malignant neoplasms has been performed on the U87 human glioblastoma *** approach,termed"Karanahan",is aimed at the eradication of cancer stem cells(CSCs),which were recently shown to be capable of internalizing fragments of extracellular double-stranded *** being internalized,these fragments interfere in the process of repairing interstrand cross-links caused by exposure to appropriate cytostatics,and such an interference results either in elimination of CSCs or in the loss of their tumorigenic *** of the approach requires a scheduled administration of cytostatic and complex composite double-stranded DNA ***:U87 cells treated in vitro in accordance with the Karanahan approach completely lost their tumorigenicity and produced no grafts upon intracerebral transplantation into immunodeficient *** SCID mice with developed subcutaneous grafts,the treatment resulted in reliable slowing down of tumor growth rate(P<0.05).In the experiment with intracerebral transplantation of U87 cells followed by surgical excision of the developed graft and subsequent therapeutic treatment,the Karanahan approach was shown to reliably slow down the tumor growth rate and increase the median survival of the mice twofold relative to the ***:The effectiveness of the Karanahan approach has been demonstrated both in vitro and in vivo in treating developed subcutaneous grafts as well as orthotopic grafts after surgical excision of the tumor.

关键词： Glioblastoma U87 cell line mytomycin C cancer stem cells TAMRA

Reduction-responsive nucleic acid nanocarrier-mediated miR-22 inhibition of PI3K/AKT pathway for the treatment of patient-derived tumor xenograft osteosarcoma

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Bioactive Materials 2023年第10期28卷 376-385页

作者： Dafu Chen Chengyue Lei Weifeng Liu Meiyu Shao Meizhou Sun Jianxun Guo Jingjing Cao Jing-Jun Nie Peng Luo Yuwen Luo Bingran Yu Renxian Wang Shun Duan Fu-Jian Xu Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsNational Center for OrthopaedicsBeijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology)Ministry of EducationBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029China Department of Orthopaedic Oncology Surgery Beijing Jishuitan HospitalPeking UniversityBeijing100035China JST Sarcopenia research Centre Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China

miRNAs are important regulators of gene expression and play key roles in the development of cancer, including osteosarcoma. During the development of osteosarcoma, the expression of miR-22 is significantly downregulated, making miR-22 as a promising therapeutic target against osteosarcoma. To design and fabricate efficient delivery carriers of miR-22 into osteosarcoma cells, a hydroxyl-rich reduction-responsive cationic polymeric nanoparticle, TGIC-CA (TC), was developed in this work, which also enhanced the therapeutic effects of Volasertib on osteosarcoma. TC was prepared by the ring-opening reaction between amino and epoxy groups by one-pot method, which had the good complexing ability with nucleic acids, reduction-responsive degradability and gene transfection performance. TC/miR-22 combined with volasertib could inhibit proliferation, migration and promote apoptosis of osteosarcoma cells in vitro. The anti-tumor mechanisms were revealed as TC/ miR-22 and volasertib could inhibit the PI3K/Akt signaling pathway synergistically. Furthermore, this strategy showed outstanding tumor suppression performance in animal models of orthotopic osteosarcoma, especially in patient-derived chemo-resistant and chemo-intolerant patient-derived xenograft (PDX) models, which reduced the risk of tumor lung metastasis and overcame drug resistance. Therefore, it has great potential for efficient treatment of metastasis and drug resistance of osteosarcoma by the strategy of localized, sustained delivery of miR-22 using the cationic nanocarriers combined with non-traditional chemotherapy drugs.

关键词： Nucleic acid delivery Nanoparticle Gene therapy Osteosarcoma Responsive Patient-Derived Xenograft(PDX)

Development of fish collagen in tissue regeneration and drug delivery

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Engineered Regeneration 2022年第3期3卷 217-231页

作者： Meison Furtado Liang Chen Zehao Chen Ao Chen Wenguo Cui Department of orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025P.R.China

Tissues rely on collagen for structural and biological integrity,as well as for function and *** collagen sources have been investigated due to its wide variety of applications,such as collagen from cows and ***,mammalian-based collagen has been limited by diseases like bovine spongiform encephalopathy(BSE)and other religious ***,fish collagen has caught the attention of the research community because it is easy to extract,has a high level of collagen content,excellent absorption properties,a low molecular weight,biocompatibility,little risk of disease transmission from animals to humans,negligible environmental contamination,and fewer ethical and religious concerns,posing as an ideal resource for product *** review focuses on the growing role of marine collagen in the advances of various biomedical applications,such as drug delivery,tissue engineering,regeneration,and wound healing,which will be covered in depth.

关键词： Collagen Fish collagen Marine collagen Drug delivery Tissue engineering

Role of Cu element in biomedical metal alloy design

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Rare Metals 2019年第6期38卷 476-494页

作者： Er-Lin Zhang Shan Fu Ruo-Xian Wang Hai-Xia Li Ying Liu Zhi-Qiang Ma Guang-Kun Liu Chen-Shun Zhu Gao-Wu Qin Da-Fu Chen School of Materials Science and Engineering Key Laboratory for Anisotropy and Texture of MaterialsEducation Ministry of ChinaNortheastern UniversityShenyang 110819China Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Orthopaedics and TraumatologyBeijing Jishuitan HospitalBeijing 100035China

Biomedical metals are widely used as implant materials in the human or animal body to repair organs and restore function, such as heart valves, meninges, peritoneum and artificial *** element affects the microstructure, mechanical property, corrosion resistance and wear resistance, but also influences the antibacterial and biological ***, antibacterial metal alloys have shown great potential as a new kind of biomedical materials, in which Cu has been widely used as antibacterial agent *** addition, biodegradable metal alloys, including magnesium alloy and zinc alloy, also have attracted much attention *** was also used as alloying element to adjust the degradation ***, the role of Cu in the alloy design will be very important for the development of new *** this paper, we summarized the recent research results on the Cu-containing metal alloy for biomedical application and hoped that this review would give more suggestions for the further development of biomedical metal alloy.

关键词： Cu Biomedical metal alloy Corrosion resistance Antibacterial property Biological property

Predictors of early outcome in unstable pelvic fractures

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Chinese Journal of traumatology 2013年第2期16卷 94-98页

作者： Ramesh K. Sen Nirmal Raj Gopinathan Tajir Tamuk Rajesh Kumar Vibhu Krishnan Radheshyam Sament Department of orthopaedics and traumatology Postgraduate Institute of Medical Education and Research Chandigarh India

Objective： To define the preoperative and intraoperative variables which may affect the immediate postoperative outcome in surgically managed patients with unstable pelvic fractures. Methods： This study was performed prospectively from January 2009 to June 2011 on 36 consecutive patients admitted to the trauma ward of Postgraduate institute of Medical Education and research, Chandigarh, with unstable pelvic injuries. Results： In the present study of 36 patients, 29 were managed surgically. Surgical duration was 2 hours in pa- tients operated on within 1 week and 3.4 hours in those operated on after 1 week. The blood loss was 550 ml when surgery was done after a week, but when done within a week it was 350 ml. The average blood loss through Pfanenstial approach was 360 ml, through posterior approach was 408 ml and through combined approach was 660 ml which was significantly high. Conclusion： Anterior approach to the pelvis would cause significantly more amount of blood loss than poste- rior approach and extemal fixation. Surgical approaches do not have any influence on the surgical duration or the infec- tion rate. The blood loss significantly increases when the surgical time is more than 1 h. The infection rate is not influ- enced by the duration of surgery. Presence or absence of associated injuries to the head, chest or abdomen is the main determinants of patient＇s survival and it greatly influ- ences the duration of hospital stay.

关键词： Pelvis Fractures, bone Treatmentoutcome Pubic symphysis

A novel biomedical titanium alloy with high antibacterial property and low elastic modulus

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Journal of Materials Science & Technology 2021年第22期81卷 13-25页

作者： Diangeng Cai Xiaotong Zhao Lei Yang Renxian Wang Gaowu Qin Da-fu Chen Erlin Zhang Key Laboratory for Anisotropy and Texture of Materials Education Ministry of ChinaSchool of Materials Science and EngineeringNortheastern UniversityShenyang 110819China research Center for Metallic Wires Northeastern UniversityShenyang 110819China Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Orthopaedics and TraumatologyBeijing Jishuitan HospitalBeijing 100035China

β-type titanium alloys have attracted much attention as implant materials because of their low elastic modulus and high strength,which is closer to human bones and can avoid the problem of stress fielding and extend the lifetime of ***,other issues,such as the infection or inflammation postimplantation,still trouble the titanium alloy's clinical *** this paper,we developed a novel nearβ-titanium alloy(Ti-13Nb-13Zr-13Ag,TNZA)with low elastic modulus and strong antibacterial ability by the addition of Ag element followed by proper microstructure controlling,which could reduce the stress shielding and bacterial infections *** microstructure,mechanical properties,corrosion resistance,antibacterial properties and cell toxicity were studied using SEM,electrochemical testing,mechanical test and cell *** results have demonstrated that TNZA alloy exhibited an elastic modulus of 75-87 GPa and a strong antibacterial ability(up to 98%reduction)and good ***,it was also shown that this alloy's corrosion resistance was better than that of *** the results suggested that Ti-13Nb-13Zr-13Ag might be a competitive biomedical titanium alloy.

关键词： Low Young’s modulus Antibacterial property Titanium alloy Compatibility Antibacterial titanium alloy

“Genetic scissors”CRISPR/Cas9 genome editing cutting-edge biocarrier technology for bone and cartilage repair

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Bioactive Materials 2023年第4期22卷 254-273页

作者： Chao Li Yawei Du Tongtong Zhang Haoran Wang Zhiyong Hou Yingze Zhang Wenguo Cui Wei Chen Department of orthopaedics The Third Hospital of Hebei Medical UniversityOrthopaedic Research Institution of Hebei ProvinceNHC Key Laboratory of Intelligent Orthopaedic EquipmentNo.139 Ziqiang RoadShijiazhuang050051PR China Department of orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University School of Medicine197 Ruijin 2nd RoadShanghai200025PR China

CRISPR/Cas9 is a revolutionary genome editing technology with the tremendous advantages such as precisely targeting/shearing ability,low cost and convenient operation,becoming an efficient and indispensable tool in biological *** a disruptive technique,CRISPR/Cas9 genome editing has a great potential to realize a future breakthrough in the clinical bone and cartilage repairing as *** review highlights the research status of CRISPR/Cas9 system in bone and cartilage repair,illustrates its mechanism for promoting osteogenesis and chondrogenesis,and explores the development tendency of CRISPR/Cas9 in bone and cartilage repair to overcome the current limitations.

关键词： CRISPR/Cas9 Genome editing Bone repair Cartilage repair