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The Arabidopsis Homolog of the Mammalian OS-9 Protein Plays a Key Role in the Endoplasmic Reticulum-Associated Degradation of Misfolded Receptor-Like Kinases

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Molecular Plant 2012年第4期5卷 929-940页

作者： Wei Su Yidan Liu Yang Xia Zhi Honga Jianming Li Department of Molecular Cellular and Developmental Biology University of Michigan 830 N University Ann Arbor MI 48109-1048 USA present address. State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China present address. State Key Laboratory of pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing 210093 China

The endoplasmic reticulum-associated degradation （ERAD） is a highly conserved mechanism to remove mis- folded membrane/secretory proteins from the endoplasmic reticulum （ER）. While many of the individual components of the ERAD machinery are well characterized in yeast and mammals, our knowledge of a plant ERAD process is rather limited. Here, we report a functional study of an Arabidopsis homolog （AtOS9） of an ER luminal lectin Yos9 （OS-9 in mammals） that recognizes a unique asparagine-linked glycan on misfolded proteins. We discovered that AtOS9 is an ER-Iocalized glyco- protein that is co-expressed with many known/predicted ER chaperones. AT-DNA insertional atos9-t mutation blocks the degradation of a structurally imperfect yet biochemically competent brassinosteroid （BR） receptor bril-9, causing its increased accumulation in the ER and its consequent leakage to the cell surface responsible for restoring the BR sensitivity and suppressing the dwarfism of the bril-9 mutant. In addition, we identified a missense mutation in AtOS9 in a recently discovered ERAD mutant ems-rnutagenized bril suppressor 6 （ebs6-1）. Moreover, we showed that atos9-t also inhibits the ERAD of bril-5, another ER-retained BR receptor, and a misfolded EFR, a BRIl-like receptor for the bacterial translation elongation factor EF-Tu. Furthermore, we found that AtOS9 interacted biochemically and genetically with EBS5, an Arabidopsis homolog of the yeast Hrd3/mammalian SellL known to collaborate with Yos9/OS-9 to select ERAD clients. Taken together, our results demonstrated a functional role of AtOS9 in a plant ERAD process that degrades misfolded receptor-like kinases.

关键词： brassinosteroid receptor ER quality control EMS-mutagenized bril-9 suppressor, lectin N-glycan MRHdomain.

SYNTHESIS OF 11 α-HYDROXY-AND 11β-CHLORO-ARTEMISININ

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Chinese Chemical Letters 1993年第2期4卷 99-100页

作者： Ying Li De Wen LU Hui Bin ZHANG Shanghai Institute of Materia Medica Academia SinicaShanghai 200031 present address.Department of Chemistry Changshu College.Changshu 215500 present address.Division of Medicinal Chemistry Chinese Pharmaceutical UniversityNanjing 210009

11α-Hydroxy-and 11β-chloro-artemisinin have been prepared from artemisinin

关键词： CHLORO-ARTEMISININ HYDROXY-AND 11 SYNTHESIS OF 11 OH 429

Construction of RNA nanotubes

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Nano Research 2019年第8期12卷 1952-1958页

作者： Hui Li Shaoying Wang Zhouxiang Ji Congcong Xu Lyudmila S. Shlyakhtenko Peixuan Guo Center for RNA Nanobiotechnology and Nanomedicine Division of Pharmaceutics and Pharmaceutical ChemistryCollege of Pharmacy/Department of Physiology & Cell BiologyCollege of Medicine/Dorothy M. Davis Heart and Lung Research Institute and James Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA UNMC Nanoimaging Core Facility Department of Pharmaceutical SciencesCollege of Pharmacy University of Nebraska Medical CenterOmahaNE68182USA present address. University of California San FranciscoCA94158USA present address. P&Z Biological Technology NewarkNJ07103USA

Nanotubes are miniature materials with significant potential applications in nanotechnological, medical, biological and material sciences. The quest for manufacturing methods of nano-mechanical modules is in progress. For example, the application of carbon nanotubes has been extensively investigated due to the precise width control, but the precise length control remains challenging. Here we report two approaches for the one-pot self-assembly of RNA nanotubes. For the first approach, six RNA strands were used to assemble the nanotube by forming a 11 nm long hollow channel with the inner diameter of 1.7 nm and the outside diameter of 6.3 nm. For the second approach, six RNA strands were designed to hybridize with their neighboring strands by complementary base pairing and formed a nanotube with a six-helix hollow channel similar to the nanotube assembled by the first approach. The fabricated RNA nanotubes were characterized by gel electrophoresis and atomic force microscopy (AFM), confirming the formation of nanotube-shaped RNA nanostructures. Cholesterol molecules were introduced into RNA nanotubes to facilitate their incorporation into lipid bilayer. Incubation of RNA nanotube complex with the free-standing lipid bilayer membrane under applied voltage led to discrete current signatures. Addition of peptides into the sensing chamber revealed discrete steps of current blockage. Polyarginine peptides with different lengths can be detected by current signatures, suggesting that the RNA-cholesterol complex holds the promise of achieving single molecule sensing of peptides.

关键词： RNA nanotechnology peptide sensing RNA nanotube nanobiotechnology

Detection of PRMT1 inhibitors with stopped flow fluorescence

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Signal Transduction and Targeted Therapy 2018年第1期3卷 274-283页

作者： Kun Qian Hao Hu Hui Xu Y George Zheng Department of pharmaceutical and Biomedical Sciences University of GeorgiaAthensGA 30602USA present address.The Chemical Proteomics Center and State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Protein arginine methyltransferases(PRMTs)are crucial epigenetic regulators in eukaryotic organisms that serve as histone writers for chromatin *** also methylate a variety of non-histone protein substrates to modulate their function and *** development of potent PRMT inhibitors has become an emerging and imperative research area in the drug discovery field to provide novel therapeutic agents for treating diseases and as tools to investigate the biological functions of ***1 is the major type I enzyme that catalyzes the formation of asymmetric dimethyl arginine,and PRMT1 plays important regulatory roles in signal transduction,transcriptional activation,RNA splicing,and DNA *** expression of PRMT1 is found in many types of cancers,pulmonary diseases,cardiovascular disease,diabetes,and renal ***1 is a highly promising target for therapeutic *** created a stopped flow fluorescence-based assay for PRMT1 inhibitor detection and characterization that has the advantages of being homogeneous,nonradioactive,and mix-and-measure in nature,allowing for continuous measurement of the methylation reaction and its *** our knowledge,this is the first continuous assay for PRMT1 reaction detection and inhibitor *** approach is not only capable of quantitatively determining the potency(IC50)of PRMT1 inhibitors but can also distinguish cofactor-competitive inhibitors,substrate-competitive inhibitors,and mixed-type inhibitors.

关键词： PRMT1 stopped competitive

Bimetallic PdCo catalyst for selective direct formylation of amines by carbon monoxide

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Nano Research 2017年第3期10卷 890-896页

作者： Yifeng Chen Junjie Mao Rongan Shen Dingsheng Wang Qing Peng Zhixin Yu Huifang Guo Wei He School of pharmaceutical Sciences Tsinghua University Beijing 100084 China Department of Chemistry Tsinghua University Beijing 100084 China Department of Petroleum Engineering University of Stavanger Stavanger 4036 Norway present address. Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050 China

A highly efficient and selective bimetallic Pd0.88Co0.12 nanoparticle catalyst was developed for the direct N-formylation of amines by carbon monoxide. This catalyst is compatible with a wide range of substrates, affo... 详细信息

关键词： nanoparticle formylation amines carbon monoxide bimetallic

Nanostructured Na-ion and Li-ion anodes for battery application： A comparative overview

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Nano Research 2017年第12期10卷 3942-3969页

作者： Ivana Hasa Jusef Hassoun Stefano Passerini Helmholtz Institute UIm HelmholtzstraBe 11 UIm 89081 Germany Karlsruhe Institute of Technology (KIT) PO Box 3640 Karlsruhe 76021 Germany Department of Chemical and pharmaceutical Sciences University of Ferrara via Fossato di Mortara Ferrara 44121 Italy present address. Energy Storage and Distributed Resources Division Lawrence Berkeley National Laboratory I Cyclotron Road Berkeley CA 94720 USA

This paper offers a comprehensive overview on the role of nanostructures in the development of advanced anode materials for application in both lithium and sodium-ion batteries. In particular, this review highlights the differences between the two chemistries, the critical effect of nanosize on the electrode performance, as well as the routes to exploit the inherent potential of nanostructures to achieve high specific energy at the anode, enhance the rate capability, and obtain a long cycle life. Furthermore, it gives an overview of nanostructured sodium- and lithium-based anode materials, and presents a critical analysis of the advantages and issues associated with the use of nanotechnology.

关键词： nanomaterials sodium-ion batteries lithium-ion batteries anodes nanostructured materials

Hidden in the parts:How the cell-type perspective reveals novel insights into plant-microbe interactions

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Molecular Plant 2023年第9期16卷 1359-1362页

作者： Christian Froschel Wolfgang Droge-Laser pharmaceutical Biology Julius-von-Sachs-InstituteJulius-Maximilians-Universitat WurzburgJulius-von-Sachs Platz 297082 WurzburgGermany present address.Botany ll Julius-von-Sachs-lnstituteJulius-Maximilians-Universitat WurzburgJulius-von-Sachs Platz 397082 WurzburgGermany

The origin of multicellularity is considered an evolutionary milestone,as cellular differentiation and cooperation have allowed the emergence of specialized and unique *** crucial biological processes involve only a few cells,making it difficult to detect specific transcriptional signatures in classical bulk tissue *** information often remains hidden in the sum of thousands of cells,and thus localized studies are *** plant responses to microbes are well known to occur highly localized(Emonet et al.,2021),approaches to describe their impact at a cellular level have been established only very *** single-cell transcriptomics,Verbon et al.,2023 address.d this topic and disclosed heterogeneous responses of root cells when colonized by the beneficial bacterium Pseudomonas *** on Arabidopsis,we here compare this study with some groundbreaking methodological advances and provides perspectives on upcoming endeavors(Figure 1).

关键词： Figure insight specialized

Theplant cytosolic m^(6)A RNA methylome stabilizes photosynthesis in the cold

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Plant Communications 2023年第6期4卷 70-84页

作者： Alexandre Magno Vicente Nikolay Manavski Paul Torben Rohn Lisa-Marie Schmid Antoni Garcia-Molina Dario Leister Charlotte Seydel Leo Bellin Torsten Möhlmann Gregor Ammann Stefanie Kaiser Jörg Meurer Plant Molecular Biology Faculty of BiologyLudwig-Maximilians-University MunichGroßhaderner Street 2-482152 Planegg-MartinsriedGermany Plant Development Faculty of BiologyLudwig-Maximilians-University MunichGroßhaderner Street 2-482152 Planegg-MartinsriedGermany Plant Physiology Faculty of BiologyUniversity of KaiserslauternErwin-Schröodinger-Street767663 KaiserslauternGermany pharmaceutical Chemistry Goethe University FrankfurtMax-von-Laue-Str.960438 Frankfurt am MainGermany present address.Loewe Biochemica GmbH Möuhlweg 2a82054 SauerlachGermany present address.Center for Research in Agricultural Genomics 08193

The sessile lifestyle of plants requires an immediate response to environmental stressors that affect photosynthesis,growth,and crop ***,we showed that three abiotic perturbations—heat,cold,and high light—triggered considerable changes in the expression signatures of 42 epitranscriptomic factors(writers,erasers,and readers)with putative chloroplast-associated functions that formed clusters of commonly expressed genes in *** expression changes under all conditions were reversible upon deacclimation,identifying epitranscriptomic players as modulators in acclimation *** dysfunctions,particularly those induced by the oxidative stress-inducing norflurazon in a largely GENOME UNCOUPLED-independent manner,triggered retrograde signals to remodel chloroplastassociated epitranscriptomic expression patterns.N6-methyladenosine(m^(6)A)is known as the most prevalent RNA modification and impacts numerous developmental and physiological functions in living *** cold treatment,expression of components of the primary nuclear m^(6)A methyltransferase complex was upregulated,accompanied by a significant increase in cellular m^(6)A mRNA *** the cold,the presence of FIP37,a core component of the writer complex,played an important role in positive regulation of thylakoid structure,photosynthetic functions,and accumulation of photosystemⅠ,the Cytb6f complex,cyclic electron transport proteins,and Curvature Thylakoid1 but not that of photosystemⅡcomponents and the chloroplast ATP *** of FIP37 affected abundance,polysomal loading,and translation of cytosolic transcripts related to photosynthesis in the cold,suggesting m^(6)Adependent translational regulation of chloroplast *** summary,we identified multifaceted roles of the cellular m^(6)A RNA methylome in coping with cold;these were predominantly associated with chloroplasts and served to stabilize photosynthesis.

关键词： m^(6)A RNA methylation cold acclimation stress response Arabidopsis thaliana photosynthesis chloroplast

HPLC Purification and Partial Amino Acid Sequence of an α-type Insect Neurotoxin From Venom of Scorpion Buthus martensi Karsch

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Chinese Science Bulletin 1993年第14期38卷 1211-1215页

作者：吉永华陆丽芳朱东亚徐科梶浦弘子 S.Terakawa 寺川(廴隹) Shanghai Institute of Physiology Academia Sinica. Shahghai 200031 PRC present address. New Drug Research Center Chinese Pharmaceutical University Nanjing 210009 PRC Shanghai Institute of Physiology Academia Sinica. Shanghai 200031 PRC National Institute for Physiological Sciences Okazaki 444 Japan

Scorpion venoms contain several kinds of neurotoxins, such as antimammalian neurotoxins, anti-insect neurotoxins and others. But most of them form a family of structurally related single chain proteins of 60—70 amino acid residues and selectively interact with voltage-dependent sodium channels in different excitable cells, only a few minipeptides of 31—39 amino acid residues are proved to block potassium channels. As a kind of molecular probe, scorpion neurotoxins have been widely used for analyzing the

关键词： scorpion Buthus martensi Karsch α-type insect neurotoxin amino acid sequence.