检索结果-南通市图书馆

A molecular Framework for the Control of Adventitious Rooting by TIR1/AFB2-Aux/IAADependent Auxin Signaling in Arabidopsis

引用

molecular plant 2019年第11期12卷 1499-1514页

作者： Abdellah Lakehal Salma Chaabouni Emilie Cavel Rozenn Le Hir Alok Ranjan Zahra Raneshan Ondrej Novak Daniel IPacurar Irene Perrone Frangois Jobert Laurent Gutierrez Laszlo Bako Catherine Bellini Umea plant Science Centre Department of Plant PhysiologyUmea University90736 UmeaSweden lnstitut Jean-Pierre Bourgin INRAAgroParisTechCNRSUniversite Paris-Saclay78000 VersaillesFrance Department of Biology Faculty of ScienceShahid Bahonar UniversityKermanIran Laboratory of Growth Regulators Faculty of SciencePalacky University and Institute of Experimental BotanyThe Czech Academy of Sciences78371 OlomoucCzech Republic Umea plant Science Centre Department of Forest Genetics and PhysiologySwedish Agriculture University90183 UmeaSweden Centre de Ressources Regionales en Biologie Moleculaire(CRRBM) Universite de Picardie Jules Verne80039 AmiensFrance present address:Centre de Ressources Regionales en Biologie Moleculaire(CRRBM) Universite de Picardie Jules Verne80039 AmiensFrance present address:Institute for Sustainable plant Protection National Research Council of ItalyTurinItaly present address:Umea plant Science Center Department of Forest Genetics and Plant PhysiologySLU90736 UmeaSweden

In Arabidopsis thaliana,canonical auxin-dependent gene regulation is mediated by 23 transcription factors from the AUXIN RESPONSE FACTOR(ARF)family that interact with auxin/indole acetic acid repressors(Aux/IAAs),which themselves form co-receptor complexes with one of six TRANSPORT INHIBITOR*!/AUXIN-SIGNALLING F-BOX(TIR1/AFB)*** combinations of co-receptors drive specific sensing outputs,allowing auxin to control a myriad of ***6 and ARF8 are positive regulators of adventitious root initiation upstream of jasmonate,but the exact auxin co-receptor complexes controlling the transcriptional activity of these proteins has remained ***,using loss-of-function mutants we show that three Aux/IAA genes,IAA6,IAA9,and IAA17,act additively in the control of adventitious root(AR)*** three IAA proteins interact with ARF6 and/or ARF8 and likely repress their activity in AR *** show that TIR1 and AFB2 are positive regulators of AR formation and TIR1 plays a dual role in the control of jasmonic acid(JA)biosynthesis and conjugation,as several JA biosynthesis genes are up-regulated in the tir1-1 *** results lead us to propose that in the presence of auxin,TIR1 and AFB2 form specific sensing complexes with IAA6,IAA9,and/or IAA17 to modulate JA homeostasis and control AR initiation.

关键词： TIR1/AFB AuxIAA jasmonate adventitious roots Arabidopsis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Classification, Expression Pattern, and E3 Ligase Activity Assay of Rice U-Box-Containing Proteins

引用

molecular plant 2008年第5期1卷 800-815页

作者： Li-Rong Zenga Chan Ho Park R.C. Venu Julian Gough Guo-Liang Wang Department of plant Pathology Plant Molecular Biology and Biotechnology Program The Ohio State University 2021 Coffey Rd 201 Kottman Hall Columbus OH 43210 USA present address: Boyce Thompson Institute for plant Research Tower Rd Ithaca NY 14853 USA Department of Computer Science University of Bristol Woodland Rd Bristol BS8 1UB UK Crop Gene Engineering Key Laboratory of Hunan Province Hunan Agricultural University Changsha Hunan 410128 China

Ubiquitin ligases play a central role in determining the specificity of the ubiquitination system by selecting a myriad of appropriate candidate proteins for modification. The U-box is a recently identified, ubiquitin ligase activityrelated protein domain that shows greater presence in plants than in other organisms. In this study, we identified 77 putative U-box proteins from the rice genome using a battery of whole genome analysis algorithms. Most of the U-box protein genes are expressed, as supported by the identification of their corresponding expressed sequence tags （ESTs）, full-length cDNAs, or massively parallel signature sequencing （MPSS） tags. Using the same algorithms, we identified 61 U-box proteins from the Arabidopsis genome. The rice and Arabidopsis U-box proteins were classified into nine major classes based on their domain compositions. Comparison between rice and Arabidopsis U-box proteins indicates that the majority of rice and Arabidopsis U-box proteins have the same domain organizations. The inferred phylogeny established the homology between rice and Arabidopsis U-box/ARM proteins. Cell death assay using the rice protoplast system suggests that one rice U-box gene, OsPUB51, might act as a negative regulator of cell death signaling. In addition, the selected U-box proteins were found to be functional E3 ubiquitin ligases. The identification and analysis of rice U-box proteins hereby at the genomic level will help functionally characterize this class of E3 ubiquitin ligase in the future.

关键词： ESTs MPSS U-Box-Containing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

From Squalene to Brassinolide： The Steroid Metabolic and Signaling Pathways across the plant Kingdom

引用

molecular plant 2013年第6期6卷 1738-1757页

作者： Cdcile Vriet Eugenia Russinova Christophe Reuzeau CropDesign NV a BASF Plant Science Company 9052 Gent Belgium present address: Laboratoire de G~n~tique et-de Biophysique c/es plantes Aix-Marseille UniversitY Marseille F-13009 France present address: Centre National de la Recherche Scientifique Unite Mixte de Recherche Biologie Vc~gEtale et de Microbiologie Environmentale MarseilleF-13009 France present address: Commissariat TEnergie Atomique Direction des Sciences du Vivant Institut de Biologie Environnementale et Biotechnologie MarseilleF-13009 France Department of plant Systems Biology VIB 9052 Gent Belgium Department of plant biotechnology and Bioinformatics Ghent University 9052 Gent Belgium

The plant steroid hormones, brassinosteroids （BRs）, and their precursors, phytosterols, play major roles in plant growth, development, and stress tolerance. Here, we review the impressive progress made during recent years in elucidating the components of the sterol and BR metabolic and signaling pathways, and in understanding their mecha- nism of action in both model plants and crops, such as Arabidopsis and rice. We also discuss emerging insights into the regulations of these pathways, their interactions with other hormonal pathways and multiple environmental signals, and the putative nature of sterols as signaling molecules.

关键词： Arabidopsis rice phytosterols brassinosteroids.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana

引用

molecular plant 2015年第8期8卷 1175-1187页

作者： Marta Zwiewka Tomasz Nodzynski Stephanie Robert Steffen Vanneste Jiri Friml Mendel Centre for plant Genomics and Proteomics Central European Institute of Technology (CEITEC) Masaryk University Kamenice 5 CZ-625 00 BrnoCzech Republic Department of plant Systems Biology VlB and Department of Plant Biotechnology and Bioinformatics Ghent University Technologiepark 927 9052 Gent Belgium Institute of Science and Technology (IST) Austria Am Campus 1 3400 Klosterneuburg Austria present address: Department of Forest Genetics and plant physiology Swedish University of Agricultural Sciences/Ume~. Plant Science Center 901 83 UmeaSweden

The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in os- motic conditions disrupt the equilibrium of the plasma membrane： hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root mer- istem cells. Acute hyperosmotic treatments （ionic and nonionic） enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presum- ably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin- mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.

关键词： osmotic stress protein trafficking clathrin-mediated endocytosis auxin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Primary carbohydrate metabolism genes participate in heat-stress memory at the shoot apical meristem of Arabidopsis thaliana

引用

molecular plant 2021年第9期14卷 1508-1524页

作者： Justyna Jadwiga Olas Federico Apelt Maria Grazia Annunziata Sheeba John Sarah Isabel Richard Saurabh Gupta Friedrich Kragler Salma Balazadeh Bernd Mueller-Roeber University of Potsdam Institute of Biochemistry and BiologyKarl-Liebknecht-Straße 24-25Haus 2014476 PotsdamGermany Max Planck Institute of molecular plant physiology Am Muehlenberg 114476 PotsdamGermany present address:Leiden University PO Box 95002300 RA Leidenthe Netherlands

In plants, the shoot apical meristem (SAM) is essential for the growth of aboveground organs. However, little is known about its molecular responses to abiotic stresses. Here, we show that the SAM of Arabidopsis thaliana displays an autonomous heat-stress (HS) memory of a previous non-lethal HS, allowing the SAM to regain growth after exposure to an otherwise lethal HS several days later. Using RNA sequencing, we identified genes participating in establishing the SAM's HS transcriptional memory, including the stem cell (SC) regulators CLAVATA1 (CLV1) and CLV3, HEAT SHOCK PROTEIN 17.6A (HSP17.6A), and the primary carbohydrate metabolism gene FRUCTOSE-BISPHOSPHATE ALDOLASE 6 (FBA6). We demonstrate that sugar availability is essential for survival of plants at high temperature. HEAT SHOCK TRANSCRIPTION FACTOR A2 (HSFA2A) directly regulates the expression of HSP17.6A and FBA6 by binding to the heat-shock elements in their promoters, indicating that HSFA2 is required for transcriptional activation of SAM memory genes. Collectively, these findings indicate that plants have evolved a sophisticated protection mechanism to maintain SCs and, hence, their capacity to re-initiate shoot growth after stress release.

关键词： aldolase carbon metabolism heat stress shoot apical meristem thermomemory thermopriming

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Selective Deactivation of Gibberellins below the Shoot Apex is Critical to Flowering but Not to Stem Elongation of Lolium

引用

molecular plant 2008年第2期1卷 295-307页

作者： Rod W. King Lewis N. Mander Torben Asp Colleen R MacMillan Cheryl A. Blundell Lloyd T. Evans CSIRO Plant Industry Canberra ACT 2600 Australia Research School of Chemistry Australian National University Canberra ACT 0200 Australia Department of Genetics and-biotechnology University of Aarhus 4200 Slagelse Denmark present address: ENSIS PO Box E 4008 Kingston ACT 2604 Australia

Gibberellins （GAs） cause dramatic increases in plant height and a genetic block in the synthesis of GA1 explains the dwarfing of Mendel＇s pea. For flowering, it is GAs which is important in the long-day （LD） responsive grass, Loliurn. As we show here, GA1 and GA4 are restricted in their effectiveness for flowering because they are deactivated by C-2 hydroxylation below the shoot apex. In contrast, GAs is effective because of its structural protection at C-2. Excised vegetative shoot tips rapidly degrade [14C]GA1, [14C]GA4, and [14C]GA20 （〉80% in 6 h）, but not [14C]GAs. CoincidentaUy, genes encoding two 2β-oxidases and a putative 16-17-epoxidase were most expressed just below the shoot apex （〈3 mm）. Further down the immature stem （〉4 mm）, expression of these GA deactivation genes is reduced, so allowing GA1 and GA4 to promote sub-apical stem elongation. Subsequently, GA degradation declines in florally induced shoot tips and these GAs can become active for floral development. Structural changes which stabilize GA4 confirm the link between florigenicity and restricted GA 2β-hydroxylation （e.g. 2α-hydroxylation and C-2 di-methylation）. Additionally, a 2-oxidase inhibitor （Trinexapac Ethyl） enhanced the activity of applied GA4, as did limiting C-16,17 epoxidation in 16,17-dihydro GAs or after C-13 hydroxylation. Overall, deactivation of GA1 and GA4 just below the shoot apex effectively restricts their florigenicity in Loliurn and, conversely, with GAs, C-2 and C-13 protection against deactivation allows its high florigenicity. Speculatively, such differences in GA access to the shoot apex of grasses may be important for separating floral induction from inflorescence emergence and thus could influence their survival under conditions of herbivore predation.

关键词： Selective Deactivation Shoot Apex Stem Elongation of Lolium

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A plant Immune Receptor Degraded by Selective Autophagy

引用

molecular plant 2019年第1期12卷 113-123页

作者： Fan Yang Athen N.Kimberlin Christian G.Elowsky Yunfeng Liu Ariadna Gonzalez-Solis Edgar B.Cahoon James R.Alfano Department of plant Pathology University of NebraskaLincolnNE 68588-0722USA Center for plant Science Innovation University of NebraskaLincolnNE 68588-0660USA Department of Bioohemistry University of NebraskaLincolnNE 68588-0664US Center for biotechnology University of NebraskaLincolnNE 68588-0665USA present address:Department of Biochemistry University of MissouriColumbiaMQ 65211USA present address:College of Life Science and Technology University of GuangxiNanning 530004China

plants recycle non-activated immune receptors to maintain a functional immune *** Arabidopsis immune receptor kinase FLAGELLIN-SENSING 2 (FLS2)recognizes bacterial ***,the molecular mechanisms by which non-activated FLS2 and other non-activated plant PRRs are recycled remain not well ***,we provide evidence showing thatArabidopsis orosomucoid (ORM)proteins,which have been known to be negative regulators of sphingoUpid biosynthesis,act as selective autophagy receptors to mediate the degradation of *** plants overexpressing ORM 1 or ORM2 have undetectable or greatly diminished FLS2 accumulation,nearly lack FLS2 signaling,and are more susceptible to the bacterial pathogen Pseudomonas *** the other hand,ORM1/2 RNAi plants and orml or orrn2 mutants generated by the CRISPR/Cas9-mediated gene editing have increased FLS2 accumulation and enhanced FLS2 signaling,and are more resistant to *** proteins interact with FLS2 and the autophagy-related protein ***,overexpression of ORM1 or ORM2 in autophagydefective mutants showed FLS2 abundance that is comparable to that in wild-type ***, FLS2 levels were not decreased in Arabidopsis plants overexpressing ORM1/2 derivatives that do not interact with *** together,these results suggest that selective autophagy functions in maintaining the homeostasis of a plant immune receptor and that beyond sphingolipid metabolic regulation ORM proteins can also act as selective autophagy receptors.

关键词： plant immunity selective autophagy pattern recognition receptor selective autophagy receptors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

K＋ Transporter AtCHX17 with Its Hydrophilic C Tail Localizes to Membranes of the Secretory/Endocytic System： Role in Reproduction and Seed Set

引用

molecular plant 2013年第4期6卷 1226-1246页

作者： Salil Chanroj Senthilkumar Padmanaban Daniel D. Czerny Guang-Yuh Jauh Heven Sze Department of Cell Biology and molecular Genetics and Maryland Agricultural Experiment Station University of Maryland College Park MD USA Department of plant Science and Landscape Architecture University of Maryland College Park MD USA present address: Department of biotechnology Faculty of Science Burapha University Chon-Buri 20131 Thailand Institute of plant and Microbial Biology Academia Sinica 128 Academia Road Section 2 Nankang Taipei

The importance of sorting proteins and wall materials to their destination is critical for plant growth and development, though the machinery orchestrating membrane trafficking is poorly understood. Transporters that alter the environment across endomembrane compartments are thought to be important players. Using Escherichia coli and yeast, we previously showed that several Arabidopsis Cation/H＋ eXchanger （AtCHX） members were K＋ transporters with a role in pH homeostasis, though their subcellular location and biological roles in plants are unclear. Co-expression of markers with CHX16, CHX17, CHX18, or CHX19 tagged with a fluorescent protein indicated these transporters associated with plasma membrane （PM） and post-Golgi compartments. Under its native promoter, AtCHX17（l_820）-GFP localized to prevacuolar compartment （PVC） and to PM in roots. Brefeldin A diminished AtCHX17- GFP fluorescence at PM, whereas wortmannin caused formation of GFP-labeled ring-like structures, suggesting AtCHX17 trafficked among PVC, vacuole and PM. AtCHX17（1-472） lacking its carboxylic tail did not associate with PVC or PM in plant cells. Single chx17 mutant or higher-order mutants showed normal root growth and vegetative devel- opment. However, quadruple （chx16chx17chxlSchx19） mutants were reduced in frequency and produced 50%-70% fewer seeds, indicating overlapping roles of several AtCHX17-related transporters in reproduction and/or seed devel- opment. Together, our results suggest that successful reproduction and seed development depend on the ability to regulate cation and pH homeostasis by AtCHX17-1ike transporters on membranes that traffic in the endocytic and/or secretory pathways.

关键词： membrane trafficking pH homeostasis cation/proton antiporter prevacuolar compartment.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment （PVC） Function in Arabidopsis

引用

molecular plant 2013年第6期6卷 1849-1862页

作者： Tomasz Nodzynski Mugurel I. Ferarub Sibylle Hirsch Riet De Rycke Claudiu Niculaes Wout Boerjan Jelle Van Leene Geert De Jaeger Steffen Vanneste Jiri Friml Mendel Centre for plant Genomics and Proteomics Central European Institute of Technology (CEITEC) Masaryk University (MU) Kamenice 5 CZ-625 00Brno Czech Republic Department of plant Systems Biology VIB and Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium Institute of Science and Technology Austria (IST Austria) Am Campus 1 3400 Klosterneuburg Austria present address: Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences (BOKU) 1190 Vienna Austria present address: Institute of plant Biology University of Zurich 8008 ZCirich Switzerland

Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 （pat3） mutant that we identified by an epifluorescence-based for- ward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1-GFR While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compart- ments （PVC） with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A （VPS35A）, a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lyric vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting.

关键词： retromer VPS35 VPS29 prevacuolar compartment (PVC) vacuolar trafficking Arabidopsis thaliana.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mitochondrial Perturbation Negatively Affects Auxin Signaling

引用

molecular plant 2014年第7期7卷 1138-1150页

作者： Pavel Ivanov Kerchev Inge De Clercq Jordi Denecker Per Muihlenbock Robert Kumpf Long Nguyen Dominique Audenaert Wim Dejonghe Frank Van Breusegem Department of plant Systems Biology VIB Ghent University B-9052 Gent Belgium Department of plant biotechnology and Bioinformatics Ghent University B-9052 Gent Belgium present address: Department of plant Protection Biology Swedish University of Agricultural Sciences SE-230 53 Alnarp Sweden VlB Compound Screening Facility B-9052 Gent Belgium

Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial per- turbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a （2-furyl）acrylate （FAA） substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.

关键词： mitochondrial perturbation auxin signaling retrograde communication.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：