Mitochondrial Perturbation Negatively Affects Auxin Signaling

作     者：Pavel Ivanov Kerchev Inge De Clercq Jordi Denecker Per Muihlenbock Robert Kumpf Long Nguyen Dominique Audenaert Wim Dejonghe Frank Van Breusegem 

作者机构：Department of Plant Systems Biology VIB Ghent University B-9052 Gent Belgium Department of Plant Biotechnology and Bioinformatics Ghent University B-9052 Gent Belgium Present address: Department of Plant Protection Biology Swedish University of Agricultural Sciences SE-230 53 Alnarp Sweden VlB Compound Screening Facility B-9052 Gent Belgium 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2014年第7卷第7期

页      面：1138-1150页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by Ghent University (Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable Economy’) the Agency for Innovation by Science and Technology (IWT Phoenix project) VIB and Marie Curie (OMICS@ VIB) the Interuniversity Attraction Poles Programme supported by a Marie Currie fellowship to P.M by FWO (Fonds Wetenschappelijk Onderzoek–Vlaanderen) through a Pegasus postdoctoral grant funded to P.K by IWT through a pre-doctoral fellowship to I.D.C 

主　　题：mitochondrial perturbation auxin signaling retrograde communication. 

摘      要：Mitochondria are crucial players in the signaling and metabolic homeostasis of the plant cell. The molecular components that orchestrate the underlying processes, however, are largely unknown. Using a chemical biology approach, we exploited the responsiveness of Arabidopsis UDP-glucosyltransferase-encoding UGT74E2 towards mitochondrial per- turbation in order to look for novel mechanisms regulating mitochondria-to-nucleus communication. The most potent inducers of UGT74E2 shared a （2-furyl）acrylate （FAA） substructure that negatively affected mitochondrial function and was identified before as an auxin transcriptional inhibitor. Based on these premises, we demonstrated that perturbed mitochondria negatively affect the auxin signaling machinery. Moreover, chemical perturbation of polar auxin transport and auxin biosynthesis was sufficient to induce mitochondrial retrograde markers and their transcript abundance was constitutively elevated in the absence of the auxin transcriptional activators ARF7 and ARF19.