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Arabidopsis thaliana AtUTr7 Encodes a Golgi-Localized UDP-Glucose/UDP-Galactose Transporter that Affects Lateral Root Emergence

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Molecular Plant 2012年第6期5卷 1263-1280页

作者： Michael Handford Cecilia Rodriguez-Furlan Lorena Marchan Marcelo Segura Daniela Gomez Elena Alvarez-Buyllal Guang-Yan Xiong Markus Pauly Ariel Orellana department of Biology Faculty of Sciences University of Chile Santiago Chile present address: department of Biochemistry University of Cambridge Cambridge CB2 1QW UK Centro de Biotecnologia Vegetal Facultad de Ciencias Biologicas Universidad Andres Bello Republica 217 Santiago Chile present address: facultad de ciencias biologicas Universidad AndrOs Bello Vina del Mar Chile present address: department of Pathobiology School of Veterinary Medicine University of Pennsylvania Philadelphia PA USA Instituto de Ecologia UNAM Mexico City Mexico department of Plant and Microbial Biology University of California Berkeley CA 94720 USA FONDAP Center for Genome Regulation

Nucleotide sugar transporters （NSTs） are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function（s） of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis （Arabidopsis thaliana （L.） Heynh.）, which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein （GFP） tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carried out with a tobacco and a yeast expression system suggest that AtUTr7 is capable of transferring UDP-Gal and UDP-GIc, but not a range of other UDP- and GDP-sugars, into the Golgi lumen. Mutants lacking expression of AtUTr7 exhibited an early proliferation of lateral roots as well as distorted root hairs when cultivated at high sucrose concentrations. Furthermore, the distribution of homogalacturonan with a low degree of methyl esterification differed in lateral root tips of the mutant compared to wild-type plants, although additional analytical procedures revealed no further differences in the composition of the root cell walls. This evidence suggests that the transport of UDP-Gal and UDP-GIc into the Golgi under conditions of high root biomass production plays a role in lateral root and root hair development.

关键词： nucleotide sugars nucleotide sugar transporters Golgi apparatus cell wall.

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Social Isolation in Male Rats During Adolescence Inhibits the Wnt/β-Catenin Pathway in the Prefrontal Cortex and Enhances Anxiety and Cocaine-Induced Plasticity in Adulthood

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Neuroscience Bulletin 2020年第6期36卷 611-624页

作者： Santiago Cuesta Alejandrina Funes Alejandra M.Pacchioni Area Toxicologia Departamento de Ciencias de los Alimentos y del MedioambienteFacultad de Ciencias Bioquimicas y FarmaceuticasUniversidad Nacional de RosarioSuipacha 5312000 RosarioSanta FeArgentina Consejo Nacional de Investigaciones Cientificas y Tecnicas RosarioArgentina present address:department of Microbiology University of Texas Southwestern Medical CenterDallasTX 75390USA

In adult animals,it is well established that stress has a proactive effect on psychostimulant ***,whether only a short period of stress during adolescence can also affect cocaine responses later in life and what mechanisms are involved are ***,we showed that 5 days of social isolation during rat adolescence had a long-term impact on anxiety-like behaviors,cocaine-induced conditioned place preference,and the expression of sensitization during *** the molecular level,social isolation decreased the activity of the Wnt/β-catenin pathway in the prefrontal cortex(PFC).Furthermore,after the expression of cocaine sensitization,isolated rats showed an increase in this pathway in the nucleus ***,these findings suggest that,adolescent social isolation by altering the Wnt/β-catenin pathway in the developing PFC might increase the cocaine responses during adulthood,introducing this pathway as a novel neuroadaptation in the cortical-accumbens connection that may mediate a stress-induced increase in vulnerability to drugs.

关键词： Social isolation Adolescence Cocaine vulnerability Wnt/(i-catenin pathway

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The role of auxin during early berry development in grapevine as revealed by transcript profiling from pollination to fruit set

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Horticulture Research 2021年第1期8卷 1924-1938页

作者： Francisca Godoy Nathalie Kuhn Mindy Muñoz German Marchandon Satyanarayana Gouthu Laurent deluc Serge delrot Virginie Lauvergeat Patricio Arce-Johnson departamento de Genetica Molecular y Microbiologia Facultad de Ciencias BiologicasPontificia Universidad Catolica de ChileAlameda 340SantiagoChile facultad de ciencias Agronomicas y de los Alimentos Pontificia Universidad Catolica de Valparaiso2340025 ValparaisoChile department of Horticulture Oregon State UniversityCorvallisOR 97331USA UMR Ecophysiologie et Genomique Fonctionnelle de la Vigne ISVVUniversitéde BordeauxVillenave d´OrnonFrance

Auxin is a key phytohormone that modulates fruit formation in many fleshy fruits through the regulation of cell division and *** content rapidly increases after pollination and the manipulation in its levels may lead to the parthenocarpic *** Vitis vinifera L.,little is known about the early fruit development that encompasses from pollination to fruit ***/fertilization events trigger fruit formation,and auxin treatment mimics their effect in grape berry ***,the role of auxin in this process at the molecular level is not well *** elucidate the participation of auxin in grapevine fruit formation,morphological,reproductive,and molecular events from anthesis to fruit set were described in sequential days after *** RNA-seq analysis at four time points from anthesis to fruit set revealed that the highest percentage of genes induced/repressed within the hormone-related gene category were auxin-related *** profiling showed significant transcript variations in auxin signaling and homeostasis-related genes during the early fruit *** acetic acid and several auxin metabolites were present during this ***,application of an inhibitor of auxin action reduced cell number and the mesocarp diameter,similarly to unpollinated berries,further confirming the key role of auxin during early berry *** work sheds light into the molecular features of the initial fruit development and highlights the auxin participation during this stage in grapevine.

关键词： development. auxin fir

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First report of L1014F kdr mutation in Culex quinquefasciatus in Mexico

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Insect Science 2016年第6期23卷 829-834页

作者： Gustavo Ponce Iram P. Sanchez Selene M. Garcia Jose M. Torrado Saul Lozano-Fuentes Beatriz Lopez-Monroy Adriana E. Flores facultad de ciencias biologicas Universidad Autonoma de Nuevo Leon A v. Universidad sin Cd. Universitaria San Nicolas de los GarzaN.L. 66455 Mexico department of Microbiology Immunology and Pathology Colorado State University Fort Collins CO USA

The L1014F mutation in the voltage-sodium channel gene has been associated with resistance to DDT and pyrethroids in various arthropod species including mosquitoes. We determined the frequency of the L1014F kdr mutation in 16 field populations of Culex quinquefasciatus from Northeastern Mexico collected between 2008 and 2013. The L1014F was present in all populations analyzed with the lowest frequency （3.33%） corresponding to the population from Monclova collected in 2012, and the highest frequency （63.63%） from the Monterrey population collected in 2012. The presence of a kdr mutation in populations of Cx. quinquefasciatus from northeastern Mexico provides evidence ofpyrethroid resistance. This requires a special attention, considering that pyrethroid-based insecticides are commonly used in vector-control campaigns, especially against Aedes aegypti （L.）.

关键词： Culex quinquefasciatus insecticide resistance kdr L 1014F mutation

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Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm

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Journal of Animal Science and Biotechnology 2017年第2期8卷 370-377页

作者： J.E.Hernández-Pichardo Y.Ducolomb S.Romo M.E.Kjelland R.Fierro F.Casillas M.Betancourt División de ciencias Biológicas y de la Salud Departamento de Producción Agrícola y AnimalUniversidad Autónoma Metropolitana-XochimilcoCiudad de MexicoMexico Division de ciencias biologicas y de la Salud Departamento de Ciencias de la SaludUniversidad Autónoma Metropolitana-IztapalapaSan Rafael Atlixco 186CP 09340 Ciudad de MexicoMexico departamento de ciencias Pecuarias Facultad de EstudiosSuperiores CuautitlanUNAMCiudad de MexicoEstado de MexicoMexico Conservation Genetics&BiotechLLCValley CityNDUSA Division de ciencias biologicas y de la Salud Departamento de Biologia de la ReproduccionUniversidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico Doctorado en Biologia Experimental Universidad Autonoma Metropolitana-lztapalapaCiudad de MexicoMexico

Background： In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up（SU） or swim up ＋ zona pellucida（SU ＋ ZP） ***： Experiment 1, 4–20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation（precense of one PN）. Treatments showed similar results（54, 47, 42 %, respectively） but statistically differents（P 0.05）.Conclusions： Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU ＋ ZP increased AR/A and AR/D rates in comparison with SU in fresh and frozen-thawed sperm. According to this, in terms of fertilization rates, chemical activation after ICSI increased oocyte PN formation compared to mechanical activation. Also, fresh sperm treated with SU and SU ＋ ZP were significantly different than frozen-thawed sperm,but between sperm treatments no significant differences were obtained.

关键词： Ethanol ICSI Oocyte activation Ovine Pronucleus Zona pellucida

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A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata

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BIOCELL 2012年第2期36卷 47-55页

作者： ISRAEL A.VEGA FEdeRICO A.deLLAGNOLA JORGE A.HURST MARTÍN S.GODOY ALFREDO CASTRO-VAZQUEZ Laboratorio de Fisiologia(IHEM-CONICET) and Departamento de Morfología y Fisiología(Facultad de Ciencias MédicasUniversidad Nacional de Cuyo)Casilla de Correo 335500 MendozaArgentina Instituto de ciencias Básicas-Universidad Nacional de Cuyo.Padre Jorge Contreras 1300.Parque Gral.San Martín 5500 Mendoza departamento de Química Biológica.facultad de Farmacia y Bioquímica Universidad de Buenos Aires.Present address:Instituto de Botánica Darwinion.Labardén 200B1642HYD Buenos Aires

Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were *** extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of *** fractions obtained from silica gel column chromatography of the acetone extracts(CI,CII and CIII),were studied by positive ion fast atom bombardment-mass spectrometry(FAB–MS)and hydrogen-nuclear magnetic resonance(H-NMR).Results indicated the presence of(1)a sterol in the yellow colored CI fraction;(2)a mixture of pheophorbides a and b in the major green fraction,CII;and(3)a modified pheophorbide a in the smaller green fraction,*** extracts of the C endosymbiont did not show evidence of the occurrence of C-phycocyanin,allophycocyanin or phycoerithrin(light absorption,fluorescence emission,and electrophoresis of the protein moieties)while cyanobacterial cells(Nostoc sp.)showed evidence of C-phycocyanin and *** possible phylogenetic and functional significance of the pigments present in the C endosymbiont is discussed.

关键词： symbiosis Cyanobacteria chloroplast photosynthetic pigments phylogeny

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Multiphoton single-molecule localization by sequential excitation with light minima

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Light(Science & Applications) 2022年第4期11卷 462-465页

作者： Luciano A.Masullo Fernando D.Stefani Centro de Investigaciones en Bionanociencias(CIBION) Consejo Nacional de Investigaciones Cientifcas y Tecnicas(CONICETGodoy Cruz 2390C1425FQD Ciudad Autonoma de Buenos AiresBuenos AiresArgentina departamento de Fisica Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresGuiraldes 2620C1428EHA Ciudad Autonoma de Buenos AiresBuenos AiresArgentina present address:Max Planck Institute of Biochemistry Am Klopferspitz 1882152 MartinsriedGermany

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1-2 nm precision with moderate photon counts,enabling tracking and superresolution imaging with true molecular *** this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.

关键词： excitation photon light

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Corrosion Inhibition of Carbon Steel in Acidic Mediaby Using Actinidia deliciosa(Kiwifruit)Extract

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Advances in Materials Physics and Chemistry 2015年第11期5卷 447-457页

作者： M.I.Arias-Montoya G.F.Dominguez-Patino J.G.Gonzalez-Rodriguez J.A.Dominguez-Patino M.L.Dominguez-Patino facultad de ciencias Quimicas e Ingenieria Universidad Autonoma del Estado de MorelosCuernavacaMexico facultad de ciencias biologicas Universidad Autonoma del Estado de MorelosCuernavacaMexico Universidad Autonoma del Estado de Morelos CIICAPCuernavacaMexico

The corrosion inhibition of 1018 carbon steel in 0.5 M H2SO4 by using Actinidia deliciosa (Kiwifruit) peel extract has been investigated by using potentiodynamic polarization curves and electro-chemical impedance spectroscopy. Two kinds of extracts are investigated, one from the tender, and another one from ripe Actinidia deliciosa. Concentrations include 0, 25, 50, 75, and 100 ppm at room temperature. Results indicate that both kind of extracts acted as good corrosion inhibitors, is more efficient in the ripe extract. Corrosion inhibition efficiency increases with increasing its concentration for ripe extract, whereas for the tender Actinidia deliciosa, the higest inhibitor efficiency is obtained by adding 25 ppm and decresaing with a further increase in its concentration. Both extracts improve the passive film properties by decreasing the passive current density values. It is found that the corrosion inhibition is due to the presence of heteroatoms present in Actinidia deliciosa, mainly quercitine, which is physically adsorbed in the steel following a Frumkin type of adsorption isotherm which forms a protective film.

关键词： Green Inhibitor Acidic Corrosion Electrochemical Techniques

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Abscisic Acid Connects Phytohormone Signaling with RNA Metabolic Pathways and Promotes an Antiviral Response that Is Evaded by a Self- Controlled RNA Virus

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Plant Communications 2020年第5期1卷 111-128页

作者： Fabio Pasin Hongying Shan Beatriz García Maren Müller David San León Márta Ludman David H.Fresno Károly Fátyol Sergi Munné-Bosch Guillermo Rodrigo Juan Antonio García Centro Nacional de Biotecnología(CNB-CSIC) 28049 MadridSpain Agricultural Biotechnology Research Center Academia Sinica11529 TaipeiTaiwan departamento de Biologı´a Evolutiva Ecologı´a y Ciencias AmbientalesFacultad de Biologı´aUniversidad de Barcelona08028 BarcelonaSpain Agricultural Biotechnology Institute National Agricultural Research and Innovation Centre2100 Godoll}oHungary Institute for Integrative Systems Biology(I2SysBio) CSIC-University of Valencia46980 PaternaSpain present address:College of Horticulture and Gardening Tianjin Agricultural University300384 TianjinChina

A complex network of cellular receptors,RNA targeting pathways,and small-molecule signaling provides robust plant immunity and tolerance to *** maximize their fitness,viruses must evolve control mechanisms to balance host immune evasion and plant-damaging *** genus Potyvirus comprises plant viruses characterized by RNA genomes that encode large polyproteins led by the P1 protease.A P1 autoinhibitory domain controls polyprotein processing,the release of a downstream functional RNAsilencing suppressor,and viral ***,we show that P1Pro,a plum pox virus clone that lacks the P1 autoinhibitory domain,triggers complex reprogramming of the host transcriptome and high levels of abscisic acid(ABA)accumulation.A meta-analysis highlighted ABA connections with host pathways known to control RNA stability,turnover,maturation,and *** changes triggered by P1Pro infection or ABA showed similarities in host RNA abundance and *** and hormone treatment assays showed that ABA promotes plant resistance to potyviral ***,quantitative mathematical modeling of viral replication in the presence of defense pathways supported self-control of polyprotein processing kinetics as a viral mechanism that attenuates the magnitude of the host antiviral ***,our findings indicate that ABA is an active player in plant antiviral immunity,which is nonetheless evaded by a self-controlled RNA virus.

关键词： abscisic acid antiviral immune evasion mathematical modeling viral polyprotein processing Potyvirus RNA metabolism

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ORS1, an H2O2-Responsive NAC Transcription Factor, Controls Senescence in Arabidopsis thaliana

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Molecular Plant 2011年第2期4卷 346-360页

作者： Salma Balazadeh Miroslaw Kwasniewski Camila Caldana Mohammad Mehrnia Maria Ines Zanor Gang-Ping Xue Bernd Mueller-Roeber University of Potsdam Institute of Biochemistry and Biology KarI-Liebknecht-Straβe 24-25 Haus 20 14476 Potsdam-Golm Germany Max-Planck Institute of Molecular Plant Physiology Am Muhlenberg 1 14476 Potsdam-Golm Germany University of Silesia Department of Genetics Jagiellonska 28 40-032 Katowice Poland CSIRO Plant Industry 306 Carmody Road St Lucia QLD 4067 Australia present address: Instituto de Biologia Molecular y Celular de Rosario (IBR-CONICET). facultad de ciencias Bioquimicas y Farmaceuticas (UNR) Suipacha 530 Rosario S2002LRK Argentina

We report here that ORS1, a previously uncharacterized member of the NAC transcription factor family, controls leaf senescence in Arabidopsis thaliana. Overexpression of ORS1 accelerates senescence in transgenic plants, whereas its inhibition delays it. Genes acting downstream of ORS1 were identified by global expression analysis using transgenic plants producing dexamethasone-inducible ORSl-GR fusion protein. Of the 42 up-regulated genes, 30 （-70%） were pre- viously shown to be up-regulated during age-dependent senescence, We also observed that 32 （-76%） of the ORSl-dependent genes were induced by long-term （4 d）, but not short-term （6 h） salinity stress （150 mM NaCI）. Furthermore, expression of 16 and 24 genes, respectively, was induced after 1 and 5 h of treatment with hydrogen peroxide （H2O2）, a reactive oxygen species known to accumulate during salinity stress. ORS1 itself was found to be rapidly and strongly induced by H2O2 treatment in both leaves and roots. Using in vitro binding site selection, we determined the preferred binding motif of ORS1 and found it to be present in half of the ORSl-dependent genes. ORS1 is a paralog of ORE1/ ANACO92/AtNAC2, a previously reported regulator of leaf senescence. Phylogenetic footprinting revealed evolutionary conservation of the ORS1 and ORE1 promoter sequences in different Brassicaceae species, indicating strong positive selection acting on both genes. We conclude that ORS1, similarly to ORE1, triggers expression of senescence-associated genes through a regulatory network that may involve cross-talk with saltand H2O2-dependent signaling pathways.

关键词： NAC transcription factor leaf senescence gene expression gene regulatory network hydrogen peroxide

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