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Salinity Stress in Roots of Contrasting BarleyGenotypes Reveals Time-Distinct and Genotype-Specific Patterns for Defined Proteins

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Molecular Plant 2014年第2期7卷 336-355页

作者： Katja Witzel Andrea Matros Marc Strickert Stephanie Kaspar Manuela Peukert Karl H. Mühling Andreas Börner Hans-Peter Mock leibniz institute of Plant Genetics and Crop Plant Research Corrensstrasse 3 06466 Gatersleben Germany present address: leibniz institute of vegetable and ornamental crops Theodor-Echtermeyer-Weg 1 14979 Großbeeren Germany present address: University of Siegen Media Systems Group Hölderlinstrasse 3 57068 Siegen Germany present address: Bruker Daltonik GmbH Fahrenheitstrasse 4 28359 Bremen Germany institute of Plant Nutrition and Soil Science Christian Albrechts University Hermann-Rodewald-Strasse 2 24118 Kiel Germany

Soil salinity is one of the most severe abiotic stress factors threatening agriculture worldwide. Hence,particular interest exists in unraveling mechanisms leading to salt tolerance and improved crop plant performance onsaline soils. Barley is considered to be one of the most salinity-tolerant crops, but varying levels of tolerance are wellcharacterized. A proteomic analysis of the roots of two contrasting cultivars （cv. Steptoe and cv. Morex） is *** plants were exposed to a period of 1, 4, 7, or 10 d at 0, 100, or 150mM NaCI. The root proteome was analyzedbased on two-dimensional gel electrophoresis. A number of cultivar-specific and salinity stress-responsive proteins wereidentified. Mass spectrometry-based identification was successful for 74 proteins, and a hierarchical clustering analysisgrouped these into five clusters based on similarity of expression profile. The rank product method was applied to sta-tistically access the early and late responses, and this delivered a number of new candidate proteins underlying salinitytolerance in barley. Among these were some germin-like proteins, some pathogenesis-related proteins, and numerousas-yet uncharacterized proteins. Notably, proteins involved in detoxification pathways and terpenoid biosynthesis weredetected as early responsive to salinity and may function as a means of modulating growth-regulating mechanisms andmembrane stability via fine tuning of phytohormone and secondary metabolism in the root.

关键词： barley genotypes mass spectrometry proteome analysis salinity stress two-dimensional gel electrophoresis

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Tissue-specific signatures of metabolites and proteins in asparagus roots and exudates

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Horticulture Research 2021年第1期8卷 1044-1057页

作者： Stefanie Doll Roxana Djalali Farahani-Kofoet Rita Zrenner Andrea Henze Katja Witzel leibniz institute of Plant Biochemistry Weinberg 306120 Halle/SaaleGermany leibniz institute of vegetable and ornamental crops Theodor-Echtermeyer-Weg 114979 GroßbeerenGermany University of Potsdam Institute of Nutritional ScienceArthur-Scheunert-Allee 114-11614558 NuthetalGermany present address:German Centre for Integrative Biodiversity Research(iDiv)Halle-Jena-Leipzig Deutscher Platz 5e04103 LeipzigGermany

Comprehensive untargeted and targeted analysis of root exudate composition has advanced our understanding of rhizosphere ***,little is known about exudate spatial distribution and *** studied the specific metabolite signatures of asparagus root exudates,root outer(epidermis and exodermis),and root inner tissues(cortex and vasculature).The greatest differences were found between exudates and root *** total,263 non-redundant metabolites were identified as significantly differentially abundant between the three root fractions,with the majority being enriched in the root exudate and/or outer tissue and annotated as‘lipids and lipid-like molecules’or‘phenylpropanoids and polyketides’.Spatial distribution was verified for three selected compounds using MALDI-TOF mass spectrometry ***-specific proteome analysis related root tissue-specific metabolite distributions and rhizodeposition with underlying biosynthetic pathways and transport *** proteomes of root outer and inner tissues were spatially very distinct,in agreement with the fundamental differences between their functions and *** to KEGG pathway analysis,the outer tissue proteome was characterized by a high abundance of proteins related to‘lipid metabolism’,‘biosynthesis of other secondary metabolites’and‘transport and catabolism’,reflecting its main functions of providing a hydrophobic barrier,secreting secondary metabolites,and mediating water and nutrient *** more abundant in the inner tissue related to‘transcription’,‘translation’and‘folding,sorting and degradation’,in accord with the high activity of cortical and vasculature cell layers in growth-and development-related *** summary,asparagus root fractions accumulate specific *** expands our knowledge of tissue-specific plant cell function.

关键词： function outer signature

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