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Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance

Rice science 2024年第1期31卷 103-117,I0020-I0022页

作者： LIU Dan ZHAO Huibo WANG Zi’an XU Jing LIU Yiting WANG Jiajia CHEN Minmin LIU Xiong ZHANG Zhihai CEN Jiangsu ZHU Li HU Jiang REN Deyong GAO Zhenyu DONG Guojun ZHANG Qiang SHEN Lan LI Qing QIAN Qian HU Songping ZHANG Guangheng State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China Research Center of plant Functional Genes and tissue culture Technology College of Bioscience and BioengineeringJiangxi Agricultural UniversityNanchang 330045China Qian Qian Academician Workstation National Nanfan Research Institute(Sanya)Chinese Academy of Agricultural SciencesSanya 572024China

The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and ***,it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought *** this study,a drought-sensitive,roll-enhanced,and narrow-leaf mutant(renl1)was induced from a semi-rolled leaf mutant(srl1)by ethyl methane sulfonate(EMS),which was obtained from Nipponbare(NPB)through ***-based cloning and functional validation showed that RENL1 encodes a cellulose synthase,allelic to NRL1/*** RENL1 mutation resulted in reduced vascular bundles,vesicular cells,cellulose,and hemicellulose contents in cell walls,diminishing the water-holding capacity of *** addition,the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species(ROS)was decreased,leading to an increase in ROS after drought ***,genetic results showed that RENL1 and SRL1 synergistically regulated cell wall *** results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance,and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance,thereby realizing simultaneous improvement of multiple traits in rice.

关键词： cellulose cell wall drought tolerance leaf morphology rice

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Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第9期14卷 774-784页

作者： Nadeem Akhtar ABBASI Tariq PERVAIZ Ishfaq Ahmed HAFIZ Mehwish YASEEN Azhar HUSSAIN plant tissue culture Laboratory Department of HorticulturePir Mehr Ali Shah-Arid Agriculture University

In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%,7%,10%,12%,14% (v/v)) for 12 min and HgCl2 (0.01%,0.05%,0.10%,0.20%,0.25% (w/v)) for 2 min.A maximum survival rate of 70% was recorded after surface sterilization with 10% *** response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins,benzylaminopurine (BAP),kinetin,and N6-(2-isopentyl)adenine (2iP).Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots,while the highest shoot length was yielded by the combination of BAP 0.5 mg/L,kinetin 0.5 mg/L,and 2iP 3 mg/*** levels of cytokinins induced callogenesis,vitrification and stunted growth to some *** rhizogenesis,uniform sized micro-shoots were excised and transferred to half-strength MS medium containing *** best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.

关键词： Eriobotryajaponica Micropropagation Sterilization of Ioquat plant growth regulator Shoot proliferation Rhizogenesis

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SELECTION OF PURE LINE OF MAIZE (Zea mays) BY ANTHER culture AND OBSERVATIONS ON ITS HYBRIDS

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Science in China,Ser.B 1983年第7期26卷 725-734页

作者：吴甲林钟兰秋农方红陈曼玲张慧英郑比兰 Laboratory of plant tissue culture Guangxi Agricultural College Nanning Guangxi

Pure Lines of Maize valuable in agricultural production could be bred by anther culture. It has been proved by our work that the pure lines obtained by such means are the same as those by conventional inbreeding metho... 详细信息

关键词： Zea mays BY ANTHER culture AND OBSERVATIONS ON ITS HYBRIDS SELECTION OF PURE LINE OF MAIZE line than LINE

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Validation and performance of three scoring systems for predicting primary non-function and early allograft failure after liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2024年第5期23卷 463-471页

作者： Yu Nie Jin-Bo Huang Shu-Jiao He Hua-Di Chen Jun-Jun Jia Jing-Jing Li Xiao-Shun He Qiang Zhao General Surgery Center Department of Hepatobiliary Surgery IIResearch Center for Artificial Organ and Tissue EngineeringGuangzhou Clinical Research and Transformation Center for Artificial LiverInstitute of Regenerative MedicineZhujiang HospitalSouthern Medical UniversityGuangzhou 510515China Organ Transplant Center the First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology Guangzhou 510080China Guangdong Provincial International Cooperation Base of Science and Technology Guangzhou 510080China division of Hepatobiliary Pancreatic Surgery the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Background: Primary non-function(PNF) and early allograft failure(EAF) after liver transplantation(LT) seriously affect patient outcomes. In clinical practice, effective prognostic tools for early identifying recipients at high risk of PNF and EAF were urgently needed. Recently, the Model for Early Allograft Function(MEAF), PNF score by King's College(King-PNF) and Balance-and-Risk-Lactate(BAR-Lac) score were developed to assess the risks of PNF and EAF. This study aimed to externally validate and compare the prognostic performance of these three scores for predicting PNF and EAF. Methods: A retrospective study included 720 patients with primary LT between January 2015 and December 2020. MEAF, King-PNF and BAR-Lac scores were compared using receiver operating characteristic(ROC) and the net reclassification improvement(NRI) and integrated discrimination improvement(IDI) analyses. Results: Of all 720 patients, 28(3.9%) developed PNF and 67(9.3%) developed EAF in 3 months. The overall early allograft dysfunction(EAD) rate was 39.0%. The 3-month patient mortality was 8.6% while 1-year graft-failure-free survival was 89.2%. The median MEAF, King-PNF and BAR-Lac scores were 5.0(3.5–6.3),-2.1(-2.6 to-1.2), and 5.0(2.0–11.0), respectively. For predicting PNF, MEAF and King-PNF scores had excellent area under curves(AUCs) of 0.872 and 0.891, superior to BAR-Lac(AUC = 0.830). The NRI and IDI analyses confirmed that King-PNF score had the best performance in predicting PNF while MEAF served as a better predictor of EAD. The EAF risk curve and 1-year graft-failure-free survival curve showed that King-PNF was superior to MEAF and BAR-Lac scores for stratifying the risk of EAF. Conclusions: MEAF, King-PNF and BAR-Lac were validated as practical and effective risk assessment tools of PNF. King-PNF score outperformed MEAF and BAR-Lac in predicting PNF and EAF within 6 months. BAR-Lac score had a huge advantage in the prediction for PNF without post-transplant variables. Proper use

关键词： Primary non-function Early allograft failure Risk predicting model Liver transplantation

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Molecular cloning and characterization of two differentially expressed Cellulose synthase gene isoforms in <i>Leucaena leucocephala</i>: A pulp yielding tree species

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Advances in Bioscience and Biotechnology 2012年第1期3卷 92-100页

作者： Rishi K. Vishwakarma Sameer Srivastava Somesh Singh Bashir M. Khan plant tissue culture division National Chemical Laboratory Maharashtra India

Leucaena leucocephala is fast growing leguminous tree species, acclimatized to variety of soil and climatic conditions. It is widely used for pulp production in India. Pulp mainly consists of cellulose, which is a simple polymer of unbranched β-1, 4-linked glucan chains. The polymerization of glucose residues into a β-1, 4-linked backbone is catalysed by the enzyme cellulose synthase (CesA). Here, cDNAs encoding CesA genes from Leucaena were isolated and characterized. The two complete cDNAs of 3.228 kb and 3.222 kb encoding CesA gene from L. leucocephala were designated as Ll-7CesA (FJ871987) and Ll-8CesA (GQ267555) respectively. In-silico studies showed that Ll-7CesA has 95.2% identities and Ll- 8CesA has 95.8% identities with Acacia mangium CesA2. Phylogenetic analysis revealed significant similarity with known dicot CesA genes. The deduced amino acid sequence of both CesA genes contained the conserved D, D, D, QxxRW motif, eight membrane spanning regions and a putative zinc binding domain, which are characteristic of glycosyltransferases. DNA blot analysis suggested, CesA gene to be in multiple copies in Leucaena genome. Semi quantitative and quantitative real-time PCR expression analysis of Ll-7CesA gene showed more expression in stem than leaf and not detected in root where as Ll-8CesA gene was expressed more in stem than leaf and root. Overall Ll-8CesA was expressed in all tested tissues and could be involved in active cellulose biosynthesis.

关键词： Cellulose Biosynthesis Cellulose Synthase Expression Analysis Leucaena leucocephala Tree Species

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Aconitum heterophyllum *** Royle:A critically endangered medicinal herb with rich potential for use in medicine

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Journal of Integrative Medicine 2022年第2期20卷 104-113页

作者： Tareq A.Wani Zahoor A.Kaloo Nisar A.Dangroo plant tissue culture Laboratory Department of BotanyUniversity of KashmirSrinagarJammu and Kashmir 190006India Department of Chemistry National Institute of TechnologySrinagarJammu and Kashmir 190006India

Aconitum heterophyllum(Patrees)is a critically endangered medicinal herb of the northwestern Himalayas and has enormous pharmacological *** is the only nonpoisonous member of the genus Aconitum,and has been used as a medicinal herb since ancient *** is an important ingredient in many traditional systems of ***,it is harvested for its roots,and its medicinal properties are due to the presence of diverse bioactive secondary metabolites,commonly known as *** understanding of the pharmacological properties of this intriguing genus is continuously growing due to its broad chemical *** therapeutic uses identified by traditional medicinal practice are receiving extensive *** in vitro experimental investigations of *** have reported the analgesic,anti-inflammatory,antiarrhythmic,antiparasitic and anticancer properties,as well as its effects on the central nervous *** this review,we highlight the classification,distribution,commerce,traditional uses,phytochemistry,pharmacology and conservation measures relevant to this ***,this review includes the biosynthetic pathways of ***’s key constituents,which could be targeted to enhance the expression levels of desired metabolites via genetic *** the genomics,transcriptomics,proteomics and metabolomic aspects of this species would be helpful in developing highly designed genotypes and chemotypes of this species to be used in commercial production.

关键词： Aconitum heterophyllum Ethnopharmocology Herbal medicine Phytochemistry

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Macroalgal deep genomics illuminate multiple paths to aquatic,photosynthetic multicellularity

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Molecular plant 2024年第5期17卷 747-771页

作者： David R.Nelson Alexandra Mystikou Ashish Jaiswal Cecilia Rad-Menendez Michael J.Preston Frederik De Boever Diana C.El Assal Sarah Daakour Michael W.Lomas Jean-Claude Twizere David H.Green William C.Ratcliff Kourosh Salehi-Ashtiani division of Science and Math New York University Abu DhabiAbu DhabiUAE Center for Genomics and Systems Biology(CGSB) New York University Abu DhabiAbu DhabiUAE Biotechnology Research Center Technology Innovation InstitutePO Box 9639Masdar CityAbu DhabiUAE culture Collection of Algae and Protozoa Scottish Association for Marine ScienceObanScotlandUK National Center for Marine Algae and Microbiota Bigelow Laboratory for Ocean SciencesEast BoothbayMEUSA Laboratory of Viral Interactomes GIGA InstituteUniversity of LiegeLiegeBelgium School of Biological Sciences Georgia Institute of TechnologyAtlantaGAUSA

Macroalgae are multicellular,aquatic autotrophs that play vital roles in global climate maintenance and have diverse applications in biotechnology and eco-engineering,which are directly linked to their multicellularity ***,their genomic diversity and the evolutionary mechanisms underlying multicellularity in these organisms remain *** this study,we sequenced 110 macroalgal genomes from diverse climates and phyla,and identified key genomic features that distinguish them from their microalgal *** for cell adhesion,extracellular matrix formation,cell polarity,transport,and cell differentiation distinguish macroalgae from microalgae across all three major phyla,constituting conserved and unique gene sets supporting multicellular *** genes show phylum-and climate-specific expansions that may facilitate niche ***,our study reveals genetic determinants of convergent and divergent evolutionary trajectories that have shaped morphological diversity in macroalgae and provides genome-wide frameworks to understand photosynthetic multicellular evolution in aquatic environments.

关键词： macroalgae multicellularity comparative genomics evolution adhesome endogenous viral elements

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Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor

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Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 1027-1032页

作者： Yosephine Sri Wulan Manuhara Alfinda Novi Kristanti Edy Setiti Wida Utami Arif Yachya Laboratory of plant tissue culture Biology Department Faculty of Science and Technology Airlangga University Laboratory of Organic Chemistry Chemistry Department Faculty of Science and Technology Airlangga University Laboratory of plant tissue culture Biology Department Faculty of Mathematics and Natural Sciences PGRI Adi Buana University

Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(***)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of *** were infected by Agrobacterium rhizogenes strain *** hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L *** BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).cultures were maintained for *** and dry weights of hairy roots at the end of culture were ***:Various concentrations of sucrose influenced the biomass accumulation of hairy *** biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than *** supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than ***,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of ***:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of *** in the large scale.

关键词： Talinum paniculatum Gaertn. Saponin Hairy root Liq

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Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)

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The Crop Journal 2015年第1期3卷 46-56页

作者： Ashok A.Nikam Rachayya M. Devarumath Akash Ahuja Harinath Babu Mahadeo G.Shitole Penna Suprasanna tissue culture Section Vasantdada Sugar InstituteManjari (Bk) Pune 412307 India Molecular Biology and Genetic Engineering division Vasantdada Sugar InstituteManjari (Bk) Pune 412307 India Department of Botany Pune University plant Stress Physiology & Biotechnology Section Nuclear Agriculture & Biotechnology Division Bhabha Atomic Research Centre

Gamma ray-induced in vitro mutagenesis and selection for salt(NaC l) tolerance were investigated in sugarcane(Saccharum officinarum L.). Embryogenic callus cultures were irradiated(10 to 80 Gy) and subjected to in vitro selection by exposure of irradiated callus to NaC l(0, 50, 100,150, 200, and 250 mmol L-1). Increasing NaC l concentrations resulted in growth reduction and increased membrane damage. Salt-selected callus lines were characterized by the accumulation of proline, glycine betaine, and Na+and K+concentration. Higher accumulation of proline and glycine betaine was observed in NaC l stressed callus irradiated at 20 Gy. Na+concentration increased and K+concentration decreased with increasing salt level. Irradiated callus showed50–60% regeneration under NaC l stress, and in vitro-regenerated plants were acclimatized in the greenhouse, with 80–85% survival. A total of 138 irradiated and salt-selected selections were grown to maturity and their agronomic performance was evaluated under normal and saline conditions. Of these, 18 mutant clones were characterized for different agro-morphological characters and some of the mutant clones exhibited improved sugar yield with increased Brix%,number of millable canes, and yield. The result suggest that radiation-induced mutagenesis offers an effective way to enhance genetic variation in sugarcane.

关键词： Gamma-rays Mutagenesis Embryogenic callus Saccharum officinarum L Salt tolerance

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Gamma Irradiation of Embryogenic Callus cultures and In vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinarum L.)

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Agricultural Sciences in China 2008年第9期7卷 1147-1152页

作者： Vikas Y Patade P Suprasanna V A Bapat plant Cell culture Technology Section Nuclear Agriculture & Biotechnology DivisionBhabha Atomic Research Centre

Radiation induced mutagenesis followed by in vitro selection was employed for salt tolerance in popular Indian sugarcane （Saccharum officinarum L.） cv. CoC-671. Embryogenic calli were gamma irradiated and exposed to different levels of NaCl （42.8, 85.6, 128.3, 171.1,213.9, 256.7, 299.5, or 342.2 mM）. The relative growth rate （RGR） decreased progressively with increasing salt stress and was the least with a salt stress of 256.7 mM （0.25±0.009）, almost 10 fold lesser than the control. The RGR was significantly lower in 85.6 mM and higher salt stressed calli than the control. The survival percent also decreased, with an increase in NaCl concentration. In case of 10 and 20 Gy irradiated calli, regeneration was observed up to 85.6 mM NaCl selection, medium, whereas, higher treatments （128.3 mM and beyond） exhibited browning initially. However, in the subsequent subcultures, regeneration was obtained in the case of 10 and 20 Gy irradiated calli on 128.3 and 171.1 mM NaCl selections. Higher dose of gamma irradiation （40 Gy） also showed regeneration, but only with 85.6 mM NaCI selection. The unirradiated calli regenerated the highest number of plantlets followed by 10 and 20 Gy irradiated calli on salt selection. A total of 147 plantlets were selected from different salt levels. The salt selected plants are being tested for their field performance.

关键词： sugarcane in vitro mutagenesis in vitro selection salt tolerance

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