Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

作     者：Nadeem Akhtar ABBASI Tariq PERVAIZ Ishfaq Ahmed HAFIZ Mehwish YASEEN Azhar HUSSAIN 

作者机构：Plant Tissue Culture LaboratoryDepartment of HorticulturePir Mehr Ali Shah-Arid Agriculture University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2013年第14卷第9期

页      面：774-784页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：Project(No.20-1540/R&D/09)supported by the Higher Education Commission(HEC)of Pakistan 

主　　题：Eriobotryajaponica Micropropagation Sterilization of Ioquat Plant growth regulator Shoot proliferation Rhizogenesis 

摘      要：In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%,7%,10%,12%,14% (v/v)) for 12 min and HgCl2 (0.01%,0.05%,0.10%,0.20%,0.25% (w/v)) for 2 min.A maximum survival rate of 70% was recorded after surface sterilization with 10% *** response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins,benzylaminopurine (BAP),kinetin,and N6-(2-isopentyl)adenine (2iP).Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots,while the highest shoot length was yielded by the combination of BAP 0.5 mg/L,kinetin 0.5 mg/L,and 2iP 3 mg/*** levels of cytokinins induced callogenesis,vitrification and stunted growth to some *** rhizogenesis,uniform sized micro-shoots were excised and transferred to half-strength MS medium containing *** best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.