检索结果-南通市图书馆

Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5

Journal of Integrative Agriculture 2019年第3期18卷 688-697页

作者： YANG Chang-hong ZHANG Yang HUANG Chao-feng College of Resources and Environmental Sciences Nanjing Agricultural University Shanghai Center for plant Stress biology and National Key Laboratory of plant molecular Genetics CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences

Cadmium(Cd) intake is harmful to human health and Cd contamination in rice grains represents a severe threat to those consuming rice as a staple food. Knockout of Cd transporters is a promising strategy to reduce Cd accumulation in rice grains. OsNRAMP5 is the major transporter for Cd and manganese(Mn) uptake in rice. Nevertheless, it is uncertain whether knockout of OsNRAMP5 is applicable to produce low Cd rice without affecting plant growth and grain yield. In this study, we adopted CRISPR/Cas9-based gene editing technology to knock out OsNRAMP5 in two japonica varieties. We generated three independent transgene-free osnramp5 mutants and investigated the effect of osnramp5 mutations on Cd accumulation and plant growth. Hydroponic experiments showed that plant growth and chlorophyll content were significantly reduced in osnramp5 mutants at low Mn conditions, and this defective growth in the mutants could be fully rescued by supply of high levels of Mn. Cd and Mn accumulation in both roots and shoots was markedly reduced in the mutants compared to that in wild-type plants. In paddy field experiments, although Cd in flag leaves and grains was greatly reduced in osnramp5 mutants, some agronomic traits including plant height, seed setting rate, and grain number per panicle were affected in the mutants, which ultimately caused a mild reduction in grain yield. The reduced plant growth in the mutants can be attributed to a marked decrease in Mn accumulation. Our results reveal that the manipulation of OsNRAMP5 should be treated with caution: When assessing the applicability of osnramp5 mutants, soil pH and soil water content in paddy fields need to be taken into consideration, since they might affect the levels of available Mn in the soil and consequently determine the effect of the mutation on grain yield.

关键词： cadmium content CRISPR/Cas9 OsNRAMP5 Oryza sativa(rice)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cytochrome P450 monooxygenase-mediated eicosanoid pathway:A potential mechanistic linkage between dietary fatty acid consumption and colon cancer risk

引用

Food Science and Human Wellness 2019年第4期8卷 337-343页

作者： Weicang Wang Jianan Zhang Guodong Zhang Department of Food Science University of Massachusetts-AmherstAmherstMA01003USA molecular and cellular biology program University of Massachusetts-AmherstAmherstMA01003USA

Human consumption of linoleic acid(LA,18:2ω-6,abundant in vegetable oils)is very *** experiments showed that excessive LA intake increased azoxymethane-induced colon tumorigenesis,however,the impact of excessive LA on colon cancer in human is not conclusive,making it difficult to make dietary recommendations for optimal intake of *** the molecular mechanisms of LA on colon tumorigenesis could help to clarify its health effect,and facilitate development of mechanismbased strategies for preventing colon *** studies show that the previously unappreciated cytochrome P450 monooxygenase-mediated eicosanoid pathway is upregulated in colon cancer and plays critical roles in its pathogenesis,and could contribute to the effects of dietary LA,as well asω-3 fatty acids,on colon *** this review,we will discuss recent studies about the roles of cytochrome P450 monooxygenases in fatty acid metabolism and its roles in colonic inflammation and colon cancer,and how this information could help us to clarify the health impacts of dietary fatty acids.

关键词： Linoleic acid Polyunsaturated fatty acids ω-3 Fatty acids Colon cancer Colonic inflammation Cytochrome P450 Eicosanoids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genetic and Epigenetic Understanding of the Seasonal Timing of Flowering

引用

plant Communications 2020年第1期1卷 25-37页

作者： Yuehui He Tao Chen Xiaolin Zeng National Key Laboratory of plant molecular Genetics&Shanghai Center for plant Stress biology Chinese Academy of Sciences Center for Excellence in Molecular Plant SciencesShanghai 201602China

The developmental transition to flowering in many plants is timed by changing seasons,which enables plants to flower at a season that is favorable for seed *** plants grown at high latitudes perceive the seasonal cues of changing day length and/or winter cold(prolonged cold exposure),to regulate the expression of flowering-regulatory genes through the photoperiod pathway and/or vernalization pathway,and thus align flowering with a particular *** studies in the model flowering plant Arabidopsis thaliana have revealed that diverse transcription factors engage various chromatin modifiers to regulate several key flowering-regulatory genes including FLOWERING LOCUS C(FLC)and FLOWERING LOCUS T(FT)in response to seasonal ***,we summarize the current understanding of molecular and chromatin-regulatory or epigenetic mechanisms underlying the vernalization response and photoperiodic control of flowering in ***,the conservation and divergence of regulatory mechanisms for seasonal flowering in crops and other plants are briefly discussed.

关键词： vernalization photoperiodism flowering time FLOWERING LOCUS C FLOWERING LOCUS T chromatin modification

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

FRUCTOKINASE-LIKE PROTEIN 1 interacts with TRXz to regulate chloroplast development in rice

引用

Journal of Integrative plant biology 2018年第2期60卷 94-111页

作者： Lei He Sen Zhang Zhennan Qiu Juan Zhao Wendan Nie Haiyan Lin Zhengge Zhu Dali Zeng Qian Qian Li Zhu State Key Laboratory of Rice biology China National Rice Research Institute Hebei Key Laboratory of molecular and cellular biology Hebei Normal University

Chloroplast genes are transcribed by the plastidencoded RNA polymerase（PEP） or nucleus-encoded RNA polymerase. FRUCTOKINASE-LIKE PROTEINS（FLNs） are phosphofructokinase-B（Pfk B）-type carbohydrate kinases that act as part of the PEP complex; however, the molecular mechanisms underlying FLN activity in rice remain ***, we identified and characterized a heat-stress sensitive albino（hsa_1） mutant in rice. Map-based cloning revealed that HSA_1 encodes a putative OsFLN_2. Here, we further demonstrated that knockdown or knockout of the OsFLN_1, a close homolog of HSA_1/OsFLN_2, considerably inhibits chloroplast biogenesis and the fln_1 knockout mutants, created by clustered regularly interspaced short palindromic repeats（CRISPR） and CRISPR-associate protein_9, exhibit severe albino phenotype and seedling lethality. Moreover, OsFLN_1 localizes to the *** two-hybrid, pull-down and bimolecular fluorescencecomplementation experiments revealed that OsFLN_1 and HSA_1/OsFLN_2 interact with THIOREDOXINZ（OsTRXz） to regulate chloroplast development. In agreement with this,knockout of OsTRXz resulted in a similar albino and seedling lethality phenotype to that of the fln_1 mutants. Quantitative reverse transcription polymerase chain reaction and immunoblot analysis revealed that the transcription and translation of PEP-dependent genes were strongly inhibited in fln_1 and trxz mutants, indicating that loss of OsFLN_1, HSA_1/OsFLN_2, or OsTRXz function perturbs the stability of the transcriptionally active chromosome complex and PEP activity. These results show that OsFLN_1 and HSA_1/OsFLN_2 contribute to chloroplast biogenesis and plant growth.

关键词： FLN FRUCTOKINASE-LIKE PROTEIN 1 interacts with TRXz to regulate chloroplast development in rice HSA PEP

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Coordination of Plastid and Light Signaling Pathways upon Development of Arabidopsis Leaves under Various Photoperiods

引用

molecular plant 2012年第4期5卷 799-816页

作者： Anna Lepisto Eevi Rintamaki molecular plant biology Department of Biochemistry and Food Chemistry University of Turku FI-20014 Turku Finland

plants synchronize their cellular and physiological functions according to the photoperiod （the length of the light period） in the cycle of 24 h. Photoperiod adjusts several traits in the plant life cycle, including flowering and senes- cence in annuals and seasonal growth cessation in perennials. Photoperiodic development is controlled by the coordinated action of photoreceptors and the circadian clock. During the past 10 years, remarkable progress has been made in under- standing the molecular mechanism of the circadian clock, especially with regard to the transition of Arabidopsis from the vegetative growth to the reproductive phase. Besides flowering photoperiod also modifies plant photosynthetic struc- tures and traits. Light signals controlling biogenesis of chloroplasts and development of leaf photosynthetic structures are perceived both by photoreceptors and in chloroplasts. In this review, we provide evidence suggesting that the photope- riodic development of Arabidopsis leaves mimics the acclimation of plant to various light intensities. Furthermore, the chloroplast-to-nucleus retrograde signals that adjust acclimation to light intensity are proposed to contribute also to the signaling pathways that control photoperiodic acclimation of leaves.

关键词： acclimation chloroplast biology circadian clock leaf/vegetative development light signaling photomor-phogenesis plastid signaling.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Understanding p53 functions through p53 antibodies

引用

Journal of molecular Cell biology 2019年第4期11卷 317-329页

作者： Kanaga Sabapathy David P.Lane Laboratory of molecular Carcinogenesis Division of Cellular & Molecular Research Humphrey Oei Institute of Cancer Research National Cancer Centre Singapore Singapore 169610 Singapore Cancer and Stem Cell biology program Duke-NUS Medical School Singapore 169857 Singapore Department of Biochemistry National University of Singapore (NUS) Singapore 117597 Singapore Institute of molecular and cellular biology Singapore 138673 Singapore p53 Laboratory Singapore 138648

TP53 is the most frequently mutated gene across all cancer types. Our understanding of its functions has evolved since its discovery four decades ago. Initially thought to be an oncogene, it was later realized to be a critical tumour suppressor. A significant amount of our knowledge about p53 functions have come from the use of antibodies against its various forms. The early anti-p53 antibodies contributed to the recognition of p53 accumulation as a common feature of cancer cells and to our understanding of p53 DNA-binding and transcription activities. They led to the concept that conformational changes can facilitate p53’s activity as a growth inhibitory protein. The ensuing p53 conformational-specific antibodies further underlined p53’s conformational flexibility, collectively forming the basis for current efforts to generate therapeutic molecules capable of altering the conformation of mutant p53. A subsequent barrage of antibodies against post-translational modifications on p53 has clarified p53’s roles further, especially with respect to the mechanistic details and context-dependence of its activity. More recently, the generation of p53 mutation-specific antibodies have highlighted the possibility to go beyond the general framework of our comprehension of mutant p53-and promises to provide insights into the specific properties of individual p53 mutants. This review summarizes our current knowledge of p53 functions derived through the major classes of anti-p53 antibodies, which could be a paradigm for understanding other molecular events in health and disease.

关键词： antibodies conformation mutant p53 post-translational modifications

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification of long InDels through whole genome resequencing to fine map qIF05-1 for seed isoflavone content in soybean(Glycine max L. Merr.)

引用

Journal of Integrative Agriculture 2025年第1期24卷 85-100页

作者： Jia Jia Huan Wang Ximeng Yang Bo Chen Ruqian Wei Qibin Ma Yanbo Cheng Hai Nian Key Laboratory of plant molecular Breeding of Guangdong Province/College of Agriculture South China Agricultural UniversityGuangzhou 510642China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology South China Agricultural UniversityGuangzhou 510642China

Soybean seed isoflavones are a type of secondary metabolites that can provide health and nutrition benefits for humans. In our previous study, a stable quantitative trait locus(QTL) qIF05-1 controlling the seed isoflavone content in soybean was detected on chromosome(Chr.) 05 in a recombinant inbred line(RIL) population from a cross of Huachun 2×Wayao. In this study, the parental lines were re-sequenced using the Illumina Solexa System with deep coverage. A total of 63,099 polymorphic long insertions and deletions(InDels)(≥15 bp)were identified between the parents Huachun 2 and Wayao. The InDels were unevenly distributed on 20chromosomes of soybean, varying from 1,826 in Chr. 12 to 4,544 in Chr. 18. A total of 10,002 long InDels(15.85% of total) were located in genic regions, including 1,139 large-effect long InDels which resulted in truncated or elongated protein sequences. In the qIF05-1 region, 68 long InDels were detected between the two parents. Using a progeny recombination experiment and genotype analysis, the qIF05-1 locus was mapped into a 102.2 kb genomic region, and this region contained 12 genes. By RNA-seq data analysis, genome sequence comparison and functional validation through ectopic expression in Arabidopsis thaliana, Glyma.05G208300(described as GmEGL3), which is a basic helix-loop-helix(bHLH) transcription factor in plants, emerged as the most likely confirmed gene in qIF05-1. These long InDels can be used as a type of complementary genetic method for QTL fine mapping, and they can facilitate genetic studies and molecular-assisted selection breeding in soybean.

关键词： soybean seed isoflavone content whole genome re-sequencing long InDels fine map

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Intracellular phosphate sensing in plants

引用

molecular plant 2022年第12期15卷 1831-1833页

作者： Guojie Ma Viswanathan Satheesh Mingguang Lei Shanghai Center for plant Stress biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Phosphorus is a building block in various biomolecules such as nucleic acids,proteins,and *** also plays pivotal roles in many metabolic pathways,including photosynthesis and respiration(Bowler et al.,2010).plants take up phosphorus as inorganic phosphate(Pi),which is limited in most soils,and Pi constraints affect plant growth and development and hence agricultural *** cope with low Pi availability in soil,plants have evolved complex mechanisms to maintain Pi homeostasis at the whole-plant and cellular level,which includes Pi uptake,storage,and redistribution.

关键词： soil maintain hence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mutation analysis of large tumor suppressor genes LATS1 and LATS2 supports a tumor suppressor role in human cancer

引用

Protein & Cell 2015年第1期6卷 6-11页

作者： Tian Yu John Bachman Zhi-Chun Lai Intercollege Graduate Degree program in molecular Cellular and Integrative Biosciences The Pennsylvania State University University Park PA 16802 USA Department of biology The Pennsylvania State University University Park PA 16802 USA Department of Biochemistry and molecular biology The Pennsylvania State University University Park PA 16802 USA

In recent years, human cancer genome projects provide unprecedented opportunities for the discovery of cancer genes and signaling pathways that contribute to tumor development. While numerous gene mutations can be identified from each cancer genome, what these muta- tions mean for cancer is a challenging question to address, especially for those from less understood putative new cancer genes. As a powerful approach, in silico bioinformatics analysis could efficiently sort out mutations that are predicted to damage gene function. Such an analysis of human large tumor suppressor genes, LATS1 and LATS2, has been carried out and the results support a role of hLATS1/12 as negative growth regulators and tumor suppressors.

关键词： LATS1 & LATS2 hippo signaling cancergenome human cancer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tangled but ordered human mitochondrial tRNA maturation

引用

Science China Life Sciences 2025年

作者： Shi-Ying Yao Xiao-Long Zhou Key Laboratory of RNA Innovation Science and Engineering CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of Systems Health Science of Zhejiang Province School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences

Mitochondria are cellular organelles that originate from an endosymbiotic bacterial ancestor and are primarily responsible for ATP generation via oxidative phosphorylation (OXPHOS). OXPHOS is conducted by five mitochondrial respiratory complexes in the inner mitochondrial membrane. Human mitochondria possess a distinct genome, a small, circular, doublestranded DNA (mtDNA) of 16,569 base pairs.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：