检索结果-南通市图书馆

Precore mutation enhances viral replication to facilitate persistent infection especially in HBeAg-negative patients

引用

Virologica Sinica 2024年第2期39卷 319-330页

作者： Guixin Li Danli Yang Xin Liu Ting Zhang Hui Liu Jun Zou Zimeng Xu Xiangmei chen Lizhong Dai Hongsong chen Fengmin Lu peking university People's Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesBeijing International Cooperation Base for Science and Technology on NAFLD DiagnosisBeijing100044China Department of Microbiology and Infectious Disease center School of Basic Medical SciencesPeking UniversityBeijing100191China Baruch S.Blumberg institute DoylestownPA18901USA Shenzhen Sanyuansheng Biotechnology co. LtdShenzhen518000China Precision Medicine center of Zhengzhou university Academy of Medical SciencesZhengzhou UniversityZhengzhou450001China peking university-Sansure Biotech Joint laboratory of Molecular Medicine Sansure Biotech Co.LtdChangsha410205China

Naturally occurred precore(Pc,G1896A)and/or basal core promoter(BcP,A1762T/G1764A)mutations are prevalent in chronic HBV-infected patients,especially those under HBeAg-negative ***,the replicative capacity of HBV with Pc/BcP mutations remains ***,meta-analysis showed that,only under HBeAg-negative status,the serum HBV DNA load in patients with Pc mutation was 7.41-fold higher than those without the *** Pc mutation alone and BcPþPc mutations promoted HBV replication in cell and hydrodynamic injection mouse *** human hepatocyte chimeric mouse model,BcPþPc mutations led to elevated replicative capacity and intrahepatic core protein ***,prec RNA harboring Pc mutation could serve as mRNA to express core and P proteins,and such pgRNA-like function favored the maintenance of cccDNA pool under HBeAg-negative ***,BcPþPc mutations induced more extensive and severe human hepatocyte damage as well as activated endoplasmic reticulum stress and TNF signaling pathway in livers of chimeric *** study indicates that HBeAg-negative patients should be monitored on HBV mutations regularly and are expected to receive early antiviral treatment to prevent disease progression.

关键词： Hepatitis B virus Precore mutation Basal core promoter mutations Endoplasmic reticulum stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An Ideal Hallmark closest to complete cure of chronic Hepatitis B Patients:High-sensitivity Quantitative HBsAg Loss

引用

Journal of clinical and Translational hepatology 2023年第1期11卷 197-206页

作者： Zi-Long Wang Jia-Rui Zheng Rui-Feng Yang Lin-Xiang Huang Hong-Song chen Bo Feng peking university People’s Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesBeijingChina

In the era of antiviral therapy,the main goal of treatment has shifted from the persistent inhibition of hepatitis B virus(HBV)replication to the pursuit of serological clearance of HBs surface antigen(HBsAg).Based on the life cycle of HBV,HBsAg originates from covalently closed circular DNA(cccDNA)and integrated HBV DNA,thus reflecting their transcriptional *** HBsAg loss may mean elimination or persistent inactivity of the HBV genome including cccDNA and integrated HBV *** loss improves the recovery of abnormal immune function,which in turn,may further promote the clearance of residual *** with functional cure and the great improvement of clinical outcomes,the continuous seroclearance of high-sensitivity quantitative HBsAg may represent the complete cure of chronic hepatitis B(cHB).For many other risk factors besides HBV itself,patients with HBsAg loss still need regular *** this review,we summarized the evolution of cHB treatment,the origin of serum HBsAg,the pattern of HBsAg seroclearance,and the effect of HBsAg loss on immune function and disease *** addition,we discuss the significance of high-sensitivity HBsAg detection and its possibility as a surrogate of complete cure.

关键词： Hepatitis B virus chronic hepatitis B HBsAg Immunoassay Functional cure complete cure Biomarker

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

eALT-F:A New Non-Invasive Staging Method to Identify Medium to High-Risk Patients with Hcc from Ultra-High HBV Viral Load Population—china,2010-2023

引用

china cDc weekly 2023年第49期5卷 1107-1114页

作者： Jiarui Zheng Xiaoxiao Wang Zilong Wang Linxiang Huang Yandi Xie Suzhen Jiang Bo Feng peking university People’s Hospital Peking University Hepatology InstituteBeijingChina peking university People’s Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesBeijing International Cooperation Base for Science and Technology on NAFLD DiagnosisBeijingChina

Background:The objective of this study was to examine the clinical characteristics of individuals with ultra-high hepatitis B virus(HBV)viral load and develop a novel staging method for chronic hepatitis B(cHB)that can more effectively identify patients with medium to high hepatocellular carcinoma(Hcc)***:A total of 2,118 patients with HBV DNA>1×10^(7) IU/mL who visited peking university People’s Hospital between January 2010 and March 2023 were enrolled *** data from the first visit were obtained and *** traditional phases and new‘eALT-F’stages were compared to evaluate the risk of ***:In the overall patients,more than onethird of the patients were under 30 years ***,a small proportion of older people(>60 years)also had ultra-high HBV viral load(4.3%).9.1%and 6.7%of individuals with ultra-high HBV viral load showed FIB-4>3.25 and aMAP≥50,*** the traditional stages of cHB,which are based on HBeAg and alanine aminotransferase(ALT)[the upper limit of normal(ULN)ALT level at 40 IU/L for both men and women],regardless of phase,a certain proportion of patients were at risk of developing Hcc(4.1%,6.4%,25.0%,and 20.3%).However,in the new‘eALT-F’stages,which are based on HBeAg,ALT(the ULN of ALT level at 30 IU/L for men and 19 IU/L for women),and/or FIB-4 levels(>1.45),aMAP≥50 was only observed in chronic hepatitis patients with positive or negative HBeAg(6.4%and 22.1%,respectively).conclusions:The‘eALT-F’staging method,based on HBeAg,ALT(males:the ULN of ALT was 30 IU/L,females:19 IU/L)and/or FIB-4 levels,was more effective in identifying medium to high-risk patients with Hcc from patients with ultra-high HBV viral load than the traditional staging methods.

关键词： Patients Population Medium

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gadolinium-ethoxybenzyl-diethylenetriamine penta-acetic acidenhanced magnetic resonance imaging for evaluating fibrosis regression in chronic hepatitis c patients after direct-acting antiviral

引用

World Journal of Gastroenterology 2022年第20期28卷 2214-2226页

作者： Xiao-He Li Rui Huang Ming Yang Jian Wang Ying-Hui Gao Qian Jin Dan-Li Ma Lai Wei Hui-Ying Rao peking university hepatology institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesBeijing International Cooperation Base for Science and Technology on NAFLD DiagnosisPeking University People’s HospitalBeijing 100044China Hepatopancreatobiliary center Beijing Tsinghua Changgung HospitalTsinghua UniversityBeijing 100044China

BAcKGROUND Direct acting antiviral(DAA)therapy has enabled hepatitis c virus infection to become curable,while histological changes remain *** valid noninvasive methods can be confirmed for use in ***-ethoxybenzyl-diethylenetriamine penta-acetic acid(Gd-EOB-DTPA)is a liver-specific magnetic resonance imaging(MRI)contrast,related to liver function in the hepatobiliary phase(HBP).Whether Gd-EOB-DTPA-enhanced MRI can be used in the diagnosis and follow up of hepatic fibrosis in patients with chronic hepatitis c(cHc)has not been *** To investigate the diagnostic and follow-up values of Gd-EOB-DTPA-enhanced MRI for hepatic histology in patients with *** Patients with cHc were invited to undergo Gd-EOB-DTPA-enhanced MRI and liver biopsy before treatment,and those with paired qualified MRI and liver biopsy specimens were *** elastography(TE)and blood tests were also *** treated with DAAs who achieved 24-wk sustained virological response(SVR)underwent Gd-EOB-DTPA-enhanced MRI and liver biopsy *** signal intensity(SI)of the liver and muscle were measured in the unenhanced phase(UEP)(SI_(UEP-liver),SI_(UEP-muscle))and HBP(SI_(HBP-liver),SI_(HBP-muscle))via *** contrast enhancement index(cEI)was calculated as[(SI_(HBP-liver)/SI_(HBP-muscle))]/[(SI_(UEP-liver)/SI_(UEP-muscle))].liver stiffness measurement(LSM)was confirmed with *** markers,aspartate aminotransferase-to-platelet ratio index(APRI)and Fibrosis-4(FIB-4),were also calculated according to blood *** grade of inflammation and stage of fibrosis were evaluated with the modified histology activity index(mHAI)and Ishak fibrosis score,*** regression was defined as a≥1-point decrease in the Ishak fibrosis *** correlation between the cEI and liver pathology was *** diagnostic and follow-up values of the cEI,LSM,and serologic markers were *** Thirty-nine patients with cHc wer

关键词： Gadolinium-ethoxybenzyl-diethylenetriamine penta-acetic acid-enhanced magnetic resonance imaging contrast enhancement index Hepatitis c virus Direct acting antiviral Sustained virological response Fibrosis regression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

cD4^+cXcR5^+ T cells activate cD27^+IgG^+ B cells via IL-21 in patients with hepatitis c virus infection

引用

Hepatobiliary & Pancreatic diseases International 2016年第1期15卷 55-64页

作者： Fan-Yun Kong Bo Feng Heng-Hui Zhang Hui-Ying Rao Jiang-Hua Wang Xu cong Lai Wei peking university People’s Hospital Peking University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases

BAcKGROUND： chronic hepatitis c virus （HcV） infection causes the skewing and activation of B cell subsets, but the characteristics of IgG＋ B cells in patients with chronic hepa- titis c （cHc） infection have not been thoroughly elucidated. cD4＋cXcR5＋ follicular helper T （Tfh） cells, via interleukin （IL）-21 secretion, activate B cells. However, the role of cD4＋cXcR5＋ T cells in the activation ofIgG＋ B cells in cHc patients is not clear. METHODS： The frequency of IgG＋ B cells, including cD27-IgG＋ B and cD27＋IgG＋ B cells, the expression of the activation markers （cD86 and cD95） in IgG＋ B cells, and the percentage of circu- lating cD4＋cXcR5＋ T cells were detected by flow cytometry in cHc patients （n=70） and healthy controls （n=25）. The con- centrations of serum IL-21 were analyzed using ELISA. The role of cD4＋cXcR5＋ T cells in the activation of IgG＋ B cells was investigated using a co-culture system. RESULTS： A significantly lower proportion of cD27＋IgG＋ B cells with increased expression of cD86 and cD95 was observed in cHc patients. The expression of cD95 was negatively correlated with the percentage of cD27＋IgG＋ B cells, and it contributed to cD27＋IgG＋ B cell apoptosis. circulating cD4＋cXcR5＋ T cells and serum IL-21 were significantly increased in cHc patients. Moreover, circulating cD4＋cXcR5＋ T cells from cHc patients induced higher expressions of cD86 and cD95 in cD27＋IgG＋ B cells in a co-culture system; the blockade of the IL-21 decreased the expression levels of cD86 and cD95 in cD27＋IgG＋ B ***： HcV infection increased the frequency of cD4＋cXcR5＋ T cells and decreased the frequency of cD27＋IgG＋ B cells. cD4＋cXcR5＋ T cells activated cD27＋IgG＋ B cells via the secretion of IL-21.

关键词： chronic hepatitis c IgG+ B cells cD4+cXcR5+ T cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

RNA binding protein TIAR modulates HBV replication by tipping the balance of pgRNA translation

引用

Signal Transduction and Targeted Therapy 2023年第10期8卷 4889-4902页

作者： Ting Zhang Huiling Zheng Danjuan Lu Guiwen Guan Deyao Li Jing Zhang Shuhong Liu Jingmin Zhao Ju-Tao Guo Fengmin Lu Xiangmei chen Department of Microbiology and Infectious Disease center School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China Department of Pathology and hepatology The Ffth Medical Center of Chinese PLA General HospitalBeijing 100039China Department of Experimental Therapeutics Baruch S.Blumberg InstituteDoylestownPA 18902USA Bejing key laboratory of Hepatitis c and immunotherapy for liver diseases Peking University Hepatology InstitutePeking University People's HospitalBeijing 100044China

The pregenomic RNA(pgRNA)of hepatitis B virus(HBV)serves not only as a bicistronic message RNA to translate core protein(cp)and DNA polymerase(Pol),but also as the template for reverse transcriptional replication of viral DNA upon packaging into *** it is well known that pgRNA translates much more cp than Pol,the molecular mechanism underlying the regulation of cp and Pol translation efficiency from pgRNA remains elusive.

关键词： translate template translation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Distribution and changes in hepatitis c virus genotype in china from 2010 to 2020

引用

World Journal of clinical cases 2022年第14期10卷 4480-4493页

作者： Jia Yang Hui-Xin Liu Ying-Ying Su Zhi-Sheng Liang Hui-Ying Rao peking university hepatology institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseasesPeking University People’s HospitalBeijing 100044China Department of clinical Epidemiology and Biostatistics Peking University People’s HospitalBeijing 100044China National institute of Diagnostics and Vaccine Development in Infectious diseases School of Public HealthXiamen UniversityXiamen 361000Fujian ProvinceChina Department of Global Health School of Public HealthPeking UniversityBeijing 100044China

BAcKGROUND Hepatitis c virus(HcV)causes a large number of infections *** infections seem to be increasing according to a report of the World Health Organization in *** direct-acting antivirals are quite effective for most genotypes of the HcV,some genotypes fail to ***,the trend of genotype distribution is vital to better control the development of this *** To analyze the distribution and trends of the HcV genotype before and after the emergence of direct-acting antivirals in *** We searched all literature published in five electronic databases-china National Knowledge Infrastructure,Wan Fang Data,VIP chinese Journal Database,chinese Biomedical Literature Service System,and PubMed-from January 1,2010 to December 31,*** search strategy combined medical subject headings and free-text terms,including“hepatitis c virus”or“HcV”and“genotype”or“subtype”and“china”or“chinese”.Additional relevant articles were searched by manual *** were extracted to build a *** of the data were totaled according to regions,periods,routes of transmission,and *** percentages in various stratifications were *** There were 76110 samples from 30 provinces included in the *** 1(G1)accounted for 58.2%of cases nationwide,followed by G2,G6,G3b,G3a,unclassified and mixed infections(17.5%,7.8%,6.4%,4.9%,1.8%,and 1.2%,respectively).The constitution of genotype varied among different regions,with G6 and G3b being more common in the south and southwest,respectively(28.1%,15.4%).The past ten years have witnessed a decrease in G1 and G2 and an increase in G3 and G6 in almost all *** drug-use population had the most abundant genotypes,with G6 ranking first(33.3%),followed by G1 and G3b(23.4%,18.5%).cONcLUSION G3 and G6 pose a new challenge for HcV *** study revealed the distribution of HcV genotypes in china over the past 10 years,providing information for HcV management strategie

关键词： Hepacivirus Genotype china Drug users Direct-acting antiviral Hepatitis c virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recovery of natural killer cells is mainly in post-treatment period in chronic hepatitis c patients treated with sofosbuvir plus ledipasvir

引用

World Journal of Gastroenterology 2018年第40期24卷 4554-4564页

作者： Xiao-Xiao Wang Bi-Fen Luo Han-Ji Jiang Xu cong Qian Jin Dan-Li Ma Lai Wei Bo Feng peking university hepatology institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases Peking University People's Hospital

AIM To investigate how natural killer(NK) cells are affected in the elimination of hepatitis c virus(HcV) by sofosbuvir/ledipasvir, two highly effective direct-acting antivirals(DAAs). METHODS Thirteen treatment-na?ve and treatment-experienced chronic hepatitis c(cHc) patients were treated with sofosbuvir/ledipasvir, and NK cells were detected at baseline, weeks 2, 4, 8 and 12 during therapy, and week post of treatment(Pt)-12 and 24 after the end of therapy by multicolor flow cytometry and compared with those from 13 healthy controls. RESULTS All patients achieved sustained virological response. There was a significant decline in cD56^(bright) NK cell frequencies at week 8(P = 0.002) and week 12(P = 0.003), which were altered to the level comparable to healthy controls at week Pt-12, but no difference was observed in the frequency of cD56^(dim) NK cells. compared with healthy controls, the expression levels of NKG2A, NKp30 and cD94 on NK cells from cHc patients at baseline were higher. NKG2A, NKp30 and cD94 started to recover at week 12 and reached the levels similar to those of healthy controls at week Pt-12 or Pt-24. Before treatment, patients have higher interferon(IFN)-γ and perforin levels than healthy controls, and IFN-γ started to recover at week 8 and reached the normalized level at week Pt-12. cONcLUSION NK cells of cHc patients can be affected by DAAs, and phenotypes and function of NK cells recover not at early stage but mainly after the end of sofosbuvir/ledipasvir treatment.

关键词： Direct-acting antivirals therapy Hepatitis c virus Natural killer cells Natural killer subsets

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficacy of Real-world Entecavir Therapy in Treatment-naive chronic Hepatitis B Patients

引用

chinese Medical Journal 2017年第18期130卷 2190-2197页

作者： Yan-Di Xie Hui Ma Bo Feng Lai Wei peking university hepatology institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases Peking University People's HospitalBeijing 100044 China

Background：Entecavir （ETV） has been shown to be effective in randomized controlled trials in highly selected patients with hepatitis B virus （HBV） *** study aimed to evaluate the efficacy of ETV in chronic hepatitis B （cHB） patients in the real-world ***：A total of 233 treatment-na（i）ve,cHB patients who received at least 12 months of ETV treatment were included in this retrospective *** of virological response （VR）,hepatitis B s antigen （HBsAg） loss,hepatitis B e antigen （HBeAg） clearance/seroconversion,virological breakthrough,cirrhosis,and hepatocellular carcinoma were ***：Of 233 patients,175 patients were male,with mean age of 43 years old,and 135 patients were HBeAg *** mean baseline levels of serum alanine aminotransferase and HBV DNA in all patients were 230 U/L and 6.6 log 10 IU/ml,*** mean follow-up period was 28 *** cumulative rates of achieving VR increased from 3.4% at 3 months to 94.4% at 60 *** nonresponse occurred in 3 （1.3%） *** VR （PVR） occurred in 61 （26.2%） patients at 12 *** baseline serum HBV DNA level （hazard ratio [HR],2.054;P 〈 0.001） was an independent risk factor for *** loss did not *** cumulative rates of HBeAg clearance increased from 2.2% at 3 months to 28.2% at 60 *** was the significant determinant of HBeAg clearance （HR,0.341;P =0.026）.Age （HR,1.072;P =0.013） and PVR （HR,5.131;P =0.017） were the significant determinants of ***：ETV treatment was effective for HBV DNA suppression in this study,but HBsAg loss and HBeAg clearance/seroconversion rates were lower compared with previous clinical *** was associated with HBeAg clearance and cirrhosis.

关键词： Entecavir Hepatitis B Virus Partial Virological Response Primary Nonresponse Real-world Virological Response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Etiological features of cirrhosis inpatients in Beijing, china

引用

chinese Medical Journal 2013年第13期126卷 2430-2434页

作者： SONG Guang-jun FENG Bo RAO Hui-ying WEI Lai peking university People's Hospital Peking University Hepatology Institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases Peking University Beijing 100044 China

Background The etiological spectrum of cirrhosis has changed over the years, but our knowledge of it is limited. The present study aimed to investigate the etiological features of cirrhosis inpatients and their variation in the past 18 years in Beijing. Methods A retrospective analysis was performed on all patients with cirrhosis diagnosed for the first time in peking university People＇s Hospital from January 1, 1993, to October 25, 2010. Data were analyzed using SPSS 20.0. Results A total of 2119 cirrhosis inpatients were included in this study： 1412 （66.6%） male and 707 （33.4%） female. chronic hepatitis B accounted for 58.7%; chronic hepatitis c for 7.6%; chronic hepatitis B and hepatitis c virus co-infection for 0.8% （16 cases）; alcoholic liver disease for 9.4% （200 cases）; and autoimmune diseases for 9.4% （199 cases）. In the past 18 years, the percentage of chronic hepatitis B has decreased from 75.2% to 48.7%; alcoholic liver disease has increased from 5.1% to 10.6%; and autoimmune disease has increased from 2.2% to 12.9%. The percentages of chronic hepatitis B and alcoholic liver disease were higher among men, whereas the percentages of chronic hepatitis c, autoimmune diseases and cryptogenic cirrhosis were higher among women. conclusions chronic hepatitis B was still the most common etiology of cirrhosis in china, but the percentage has been decreasing. The percentages of alcoholic liver disease and autoimmune diseases have been increasing. The etiological spectrum of cirrhosis inpatients differed significantly according to sex.

关键词： etiology, cirrhosis, hepatitis B hepatitis c autoimmune liver disease, alcoholic liver disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：