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Spatial tumor biopsy with fluorescence PCR microneedle array

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The Innovation 2024年第1期5卷 26-32页

作者： Xiaoxuan Zhang Guopu Chen Yu Wang Yuanjin Zhao Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China oujiang laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001China Chemistry and Biomedicine Innovation Center Nanjing UniversityNanjing 210023China

Biopsy is the gold standard for tumor diagnosis,as this technology provides highly detailed and reliable information on tumorigenesis and *** the discrete wettability of desert beetles,in this study,a fluorescence polymerase chain reaction(F-PCR)microneedle array(MNA)platform is developed for efficient spatial tumor *** MNA is fabricated by the coupled strategies of bottom-up self-assembly and top-down photolithography;it comprises a hydrophobic silica nanoparticle-assembled substrate and graphene aerogel-hydrogel hybrid microneedle *** from the hydrophilicity and absorption capacity of its graphene hybrid microneedle peaks,MNA can easily penetrate tissue specimens and collect tumor nucleic acid biomarkers *** addition,because of the discrete wettability of the platform,both tissue fluids and PCR liquids can be easily removed from the substrate,and each microneedle peak is similar to an independent island for directly conducting F-PCR reactions for tumor marker *** on these advantages,the F-PCR-MNA platform is demonstrated to be ideal for detecting DNA biomarkers of lung carcinoma in standard solutions,mouse tissue samples,and clinical specimens,thus indicating its practical potential as an innovative tumor biopsy system.

关键词： needle biopsy diagnosis

SIL1 improves cognitive impairment in APP23/PS45 mice by regulating amyloid precursor protein processing and Aβ generation

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Zoological Research 2024年第4期45卷 845-856页

作者： Qunxian Wang Yanshuang Jiang Zijun Meng Xiangjun Dong Dongjie Hu Liangye Ji Weihui Zhou Weihong Song Chongqing Key laboratory of Child Neurodevelopment and Cognitive Disorders Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChildren's Hospital of Chongqing Medical UniversityChongqing 400000China Second Affiliated Hospital and Yuying Children’s Hospital Institute of AgingKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceWenzhou Medical UniversityWenzhouZhejiang 325000China oujiang laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)WenzhouZhejiang 325001China

SIL1,an endoplasmic reticulum(ER)-resident protein,is reported to play a protective role in Alzheimer’s disease(AD).However,the effect of SIL1 on amyloid precursor protein(APP)processing remains *** this study,the role of SIL1 in APP processing was explored both in vitro and in *** the in vitro experiment,SIL1 was either overexpressed or knocked down in cells stably expressing the human Swedish mutant *** the in vivo experiment,AAV-SIL1-EGFP or AAV-EGFP was microinjected into APP23/PS45 mice and their wild-type *** blotting(WB),immunohistochemistry,RNA sequencing(RNA-seq),and behavioral experiments were performed to evaluate the relevant *** indicated that SIL1 expression decreased in APP23/PS45 *** of SIL1 significantly decreased the protein levels of APP,presenilin-1(PS1),and C-terminal fragments(CTFs)of APP in vivo and in ***,knockdown of SIL1 increased the protein levels of APP,β-site APP cleavage enzyme 1(BACE1),PS1,and CTFs,as well as APP mRNA expression in 2EB2 ***,SIL1 overexpression reduced the number of senile plaques in APP23/PS45 ***,Y-maze and Morris Water maze tests demonstrated that SIL1 overexpression improved cognitive impairment in APP23/PS45 *** findings indicate that SIL1 improves cognitive impairment in APP23/PS45 mice by inhibiting APP amyloidogenic processing and suggest that SIL1 is a potential therapeutic target for AD by modulating APP processing.

关键词： Alzheimer’s disease SIL1 APP processing Cognitive impairment

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XBP1 splicing contributes to endoplasmic reticulum stress-induced human islet amyloid polypeptide up-regulation

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Genes & Diseases 2024年第5期11卷 350-359页

作者： Yun Zhang Susan Lin Jing Yao Wantong Cai Huaqiu Chen Ailikemu Aierken Zhe Wang Weihong Song National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijing 100053China Townsend Family Laboratories Department of PsychiatryThe University of British Columbia2255 Wesbrook MallVancouverBC V6T 1Z3Canada Institute of Aging Key Laboratory of Alzheimer’s Disease of Zhejiang ProvinceSchool of Mental Health and the Affiliated Kangning HospitalWenzhou Medical UniversityWenzhouZhejiang 325000China oujiang laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)WenzhouZhejiang 325001China

As a pathological hallmark of type 2 diabetes mellitus(T2DM),islet amyloid is formed by the aggregation of islet amyloid polypeptide(IAPP).Endoplasmic reticulum(ER)stress interacts with IAPP aggregates and has been implicated in the pathogenesis of *** examine the role of ER stress in T2DM,we cloned the hIAPP promoter and analyzed its promoter activity in human *** found that ER stress significantly enhanced hIAPP promoter activity and expression in human b-cells via triggering X-box binding protein 1(XBP1)*** identified a binding site of XBP1 in the hIAPP *** of this binding site by substitution or deletion mutagenesis significantly diminished the effects of ER stress on hIAPP promoter *** of XBP splicing by MKC3946 treatment inhibited ER stress-induced hIAPP up-regulation and improved human b-cell survival and *** study uncovers a link between ER stress and IAPP at the transcriptional level and may provide novel insights into the role of ER stress in IAPP cytotoxicity and the pathogenesis of T2DM.

关键词： b-cell function Endoplasmic reticulum stress Islet amyloid polypeptide Type 2 diabetes mellitus XBP1

Author correction to“Cascade two-stage tumor re-oxygenation and immune re-sensitization mediated by self-assembled albumin-sorafenib nanoparticles for enhanced photodynamic immunotherapy”[Acta Pharm Sin B(2022)4204-4223]

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Acta Pharmaceutica Sinica B 2024年第2期14卷 900-903页

作者： Zaigang Zhou Jiashe Chen Yu Liu Chunjuan Zheng Wenjuan Luo Lele Chen Shen Zhou Zhiming Li Jianliang Shen State Key laboratory of Ophthalmology Optometry and Vision ScienceSchool of Ophthalmology and OptometrySchool of Biomedical EngineeringWenzhou Medical UniversityWenzhou 325027China Department of the First Affiliated Hospital of Wenzhou Medical University Wenzhou325000China School&Hospital of Stomatology Wenzhou Medical UniversityWenzhou 325027China Wenzhou Institute University of Chinese Academy of SciencesWenzhou325000China oujiang laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)WenzhouZhejiang325000China

The authors regret that there are errors in the article Fig.8A and Fig.S22A due to the mistake of copying and pasting in the process of assembling figures and negligence in the *** it does not affect the conclusion,these are obvious *** authors have now modified as *** original data of these figures have been provided to the Editorial Office,and the corresponding authors or the Editorial Office can be contacted for original data *** authors apologize for any inconvenience caused to the journal and readers.

关键词： journal correction conclusion

Self‑Healing Dynamic Hydrogel Microparticles with Structural Color for Wound Management

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Nano-Micro Letters 2024年第11期16卷 177-190页

作者： Li Wang Xiaoya Ding Lu Fan Anne M.Filppula Qinyu Li Hongbo Zhang Yuanjin Zhao Luoran Shang Department of General Surgery Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200025People’s Republic of China Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096People’s Republic of China Pharmaceutical Sciences laboratory Åbo Akademi University20520 TurkuFinland oujiang laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001People’s Republic of China Shanghai Xuhui Central Hospital Zhongshan‑Xuhui Hospitaland the Shanghai Key Laboratory of Medical Epigeneticsthe International Co‑laboratory of Medical Epigenetics and Metabolism(Ministry of Science and Technology)Institutes of Biomedical SciencesFudan UniversityShanghai 200032People’s Republic of China

Chronic diabetic wounds confront a significant medical challenge because of increasing prevalence and difficult-healing *** is vital to develop multifunctional hydrogel dressings,with well-designed morphology and structure to enhance flexibility and effectiveness in wound *** achieve these,we propose a self-healing hydrogel dressing based on structural color microspheres for wound *** microsphere comprised a photothermal-responsive inverse opal framework,which was constructed by hyaluronic acid methacryloyl,silk fibroin methacryloyl and black phosphorus quantum dots(BPQDs),and was further re-filled with a dynamic *** dynamic hydrogel filler was formed by Knoevenagel condensation reaction between cyanoacetate and benzaldehyde-functionalized dextran(DEX-CA and DEX-BA).Notably,the composite microspheres can be applied arbitrarily,and they can adhere together upon near-infrared irradiation by leveraging the BPQDs-mediated photothermal effect and the thermoreversible stiffness change of dynamic ***,eumenitin and vascular endothelial growth factor were co-loaded in the microspheres and their release behavior can be regulated by the same ***,effective monitoring of the drug release process can be achieved through visual color *** microsphere system has demonstrated desired capabilities of controllable drug release and efficient wound *** characteristics suggest broad prospects for the proposed composite microspheres in clinical applications.

关键词： Black phosphorus Structural color Dynamic hydrogel Inverse opal Wound management

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Tumor angiogenesis and anti-angiogenic therapy

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中华医学杂志(英文版) 2024年第17期137卷 2024 Jul 25页

作者： Guo Ziheng Jing Xu Sun Xiaoting Sun Shishuo Yang Yunlong Cao Yihai Department of Pancreatic Surgery West China Hospital Sichuan University Chengdu Sichuan China Department of Microbiology Tumor and Cell Biology Karolinska Institute Stockholm Sweden oujiang laboratory (Zhejiang Lab for Regenerative Medicine Vison and Brain Health) School of Pharmaceutical Science Wenzhou Medical University Wenzhou Zhejiang China Cancer Institute the First Clinical Medical College Xuzhou Medical University Xuzhou Jiangsu China Department of Cellular and Genetic Medicine School of Basic Medical Sciences Fudan University Shanghai China

Anti-angiogenic drugs (AADs), which mainly target the vascular endothelial growth factor-A signaling pathway, have become a therapeutic option for cancer patients for two decades. During this period, tremendous clinical experience of anti-angiogenic therapy has been acquired, new AADs have been developed, and the clinical indications for AAD treatment of various cancers have been expanded using monotherapy and combination therapy. However, improvements in the therapeutic outcomes of clinically available AADs and the development of more effective next-generation AADs are still urgently required. This review aims to provide historical and perspective views on tumor angiogenesis to allow readers to gain mechanistic insights and learn new therapeutic development. We revisit the history of concept initiation and AAD discovery, and summarize the up-to-date clinical translation of anti-angiogenic cancer therapy in this field.

关键词： Angiogenesis Cancer Angiogenesis inhibitors Angiogenic factors Vascular endothelial growth factors Cancer therapy Combined modality therapy

The role of inflammasomes in human diseases and their potential as therapeutic targets

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Signal Transduction and Targeted Therapy 2024年第2期9卷 479-508页

作者： Jing Yao Keenan Sterling Zhe Wang Yun Zhang Weihong Song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijing 100053China Townsend Family Laboratories Department of PsychiatryBrain Research CenterThe University of British Columbia2255 Wesbrook MallVancouverBC V6T 1Z3Canada Key laboratory of Neurodegenerative Diseases Ministry of EducationBeijingP.R.China Zhejiang Clinical Research Center for Mental Disorders Key Laboratory of Alzheimer’s Disease of Zhejiang ProvinceSchool of Mental Health and The Affiliated Kangning HospitalInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang 325000China oujiang laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)WenzhouZhejiang 325000China

Inflammasomes are large protein complexes that play a major role in sensing inflammatory signals and triggering the innate immune *** inflammasome complex has three major components:an upstream sensor molecule that is connected to a downstream effector protein such as caspase-1 through the adapter protein *** formation typically occurs in response to infectious agents or cellular *** active inflammasome then triggers caspase-1 activation,followed by the secretion of pro-inflammatory cytokines and pyroptotic cell *** inflammasome activation and activity contribute to the development of diabetes,cancer,and several cardiovascular and neurodegenerative *** a result,recent research has increasingly focused on investigating the mechanisms that regulate inflammasome assembly and activation,as well as the potential of targeting inflammasomes to treat various *** clinical trials are currently underway to evaluate the therapeutic potential of several distinct inflammasome-targeting ***,understanding how different inflammasomes contribute to disease pathology may have significant implications for developing novel therapeutic *** this article,we provide a summary of the biological and pathological roles of inflammasomes in health and *** also highlight key evidence that suggests targeting inflammasomes could be a novel strategy for developing new disease-modifying therapies that may be effective in several conditions.

关键词： inflam therapeutic potential

De novo design of highly efficient type-Ⅰphotosensitizer based onπ-conjugated oligomer for photodynamic eradication of multidrug-resistant bacterial infections

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Chinese Chemical Letters 2024年第3期35卷 389-393页

作者： Qi Zhao Guangchao Qing Jie Yu Ying Liu Jianliang Shen Yang Luo Xingjie Zan Shengliang Li State Key laboratory of Ophthalmology Optometry and Vision ScienceSchool of Ophthalmology and OptometrySchool of Biomedical EngineeringWenzhou Medical UniversityWenzhou InstituteUniversity of Chinese Academy of SciencesOujiang Laboratory(Zhejiang Lab for Regenerative MedicineVision and Brain Health)Wenzhou 325001China College of Pharmaceutical Sciences Soochow UniversitySuzhou 215123China Center of Smart laboratory and Molecular Medicine School of MedicineChongqing UniversityChongqing 400044China College of Life Science and laboratory Medicine Kunming Medical UniversityKunming 650050China

Traditional photosensitizers show limited singlet oxygen generation in hypoxic infection lesions,which greatly suppress their performance in antibacterial ***,there still is lack of feasible design strategy for developing hypoxia-overcoming photosensitizers ***,radical generation ofπ-conjugated small molecules is efficiently manipulated by an individual selenium(Se)*** this strategy,the first proof-of-concept study of a Se-anchored oligo(thienyl ethynylene)(OT-Se)with high-performance superoxide radical(O_(2)^(·-))and hydroxyl radical(·OH)generation capability is present,and achieves efficient antibacterial activities towards the clinically extracted multidrug-resistant bacteria methicillin-resistant ***(MRSA)and carbapenem-resistant ***(CREC)at sub-micromolar concentration under a low white light irradiation(30 mW/cm^(2)).The water-dispersible OT-Se shows a good bacteria-anchoring capability,biocompatibility,and complete elimination of multidrug-resistant bacteria wound infection in *** work offers a strategy to boost type-I photodynamic therapy(PDT)performance for efficient antibacterial treatments,advancing the development of antibacterial agents.

关键词： Photodynamic therapy Antibacterial Type-Ⅰphotosensitizers Multidrug-resistant bacteria Conjugated oligomers

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Corrigendum to"A novel FGFR1 inhibitor CYY292 suppresses tumor progression,invasion,and metastasis of glioblastoma by inhibiting the Akt/GSK3β/snail signaling axis"[Genes&Diseases 11(2024)479-494]

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Genes & Diseases 2024年第3期11卷 485-490页

作者： Yanran Bi Ruiling Zheng Jiahao Hu Ruiqing Shi Junfeng Shi Yutao Wang Peng Wang Wenyi Jiang Gyudong Kim Zhiguo Liu Xiaokun Li Li Lin School of Pharmaceutical Science Wenzhou Medical UniversityWenzhou 325035China College of Pharmacy and Research Institute of Drug Development Chonnam National UniversityGwangju 61186Republic of Korea Department of Dermatology Wenzhou Hospital of Integrated Traditional Chinese and Western MedicineWenzhou 325000China Zhejiang Lab for Regenerative Medicine Oujiang LaboratoryWenzhou 325035China

The authors regret that in Figure 3C,the Western Blot(WB)image representing GAPDH levels was mistakenly chosen as the same image for ERK(indicated by the red dotted-line rectangle).We have attached the original WB strip for GAPDH to demonstrate that this was an unintentional error in image ***,we noticed that the Transwell images in the two upper panels of the right column in Figure 4J are misleading due to errors in image *** have attached the original data to show that this was also an unintentional *** assure you that these two corrections do not alter the scientificconclusionof thearticle.

关键词： GSK3β invasion metastasis