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Local injection of bone morphogenetic protein 7 promotes neuronal regeneration and motor function recovery after acute spinal cord injury

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Neural Regeneration Research 2018年第6期13卷 1054-1060页

作者： Chen Chen Guang-Chao Bai Hong-Liang Jin Kun Lei Kuan-Xin Li Department of Joint and Spine Xinjiang Production and Construction Corps Hospital Urumqi

After spinal cord injury,the number of glial cells and motor neurons expressing bone morphogenetic protein 7（BMP7）increases,indicating that upregulation of BMP7 can promote nerve ***,therefore,tested whether direct injection of BMP7 into acutely injured ratalalo createrywith 50 ng BMP7（BMP7 group）or physiological saline（control group）for 7 consecutive *** examination showed that the amplitude of N1 in motor evoked potentials（MEP）decreased after spinal cord *** 8 weeks post-operation,the amplitude of N1 in the BMP7 group was remarkably higher than that at 1 week post-operation and was higher than that of the control ***,Beattie,Bresnahan scale（BBB）scores,hematoxylin-eosin staining,and western blot assay showed that at 1,2,4 and 8 weeks post-operation,BBB scores were increased;Nissl body staining was stronger;the number of Nissl-stained bodies was increased;the number of vacuoles gradually decreased;the number of synapses was increased;and the expression of neuronal marker,neurofilament protein 200,was increased in the hind limbs of the BMP7 group compared with the control *** blot assay showed that the expression of GFAP protein in BMP7 group and control group did not change significantly and there was no significant difference between the BMP7 and control *** data confirmed that local injection of BMP7 can promote neuronal regeneration after spinal cord injury and promote recovery of motor function in rats.

关键词： nerve regeneration behavior Basso Beattie Bresnahan scale score motor evoked potential wave Nissl staining neurons glial cells neurofilament protein 200 glial fibrillary acidic protein neural regeneration

Milligan-Morgan hemorrhoidectomy with anal cushion suspension and partial internal sphincter resection for circumferential mixed hemorrhoids

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World Journal of Gastroenterology 2013年第30期19卷 5011-5015页

作者： Ming Lu Guang-Ying Shi Guo-Qiang Wang Yan Wu Yang Liu Hao Wen Department of General Surgery First Affiliated Hospital of Xinjiang Medical University Department of Gastroenterology Xinjiang Production and Construction Corps Hospital

AIM:To identify a more effective treatment protocol for circumferential mixed ***:A total of 192 patients with circumferential mixed hemorrhoids were randomized into the treatment group,where they underwent Milligan-Morgan hemorrhoidectomy with anal cushion suspension and partial internal sphincter resection,or the control group,where traditional external dissection and internal ligation were *** recovery and complications were ***:The time to wound healing was 12.96 ± 2.25 d in the treatment group shorter than 19.58 ± 2.71 d in the control *** pain rate was 58.3% in the treatment group higher than 22.9% in the control group;moderate pain rate was 33.3% in the treatment group lower than 56.3% in the control group severe pain rate was 8.4% in the treatment group lower than 20.8% in the control *** edema rate was 70.8% in the treatment group higher than 43.8% in the control group;mild local edema rate was 26% in the treatment group lower than 39.6% in the control group obvious local edema was 3.03% in the treatment group lower than 16.7% in the control *** stenosis rate was 85.4% in the treatment group higher than 63.5% in the control group;moderate stenosis rate was 14.6% in the treatment group Lower than 27.1% in the control group severe anal stenosis rate was 0% in the treatment group lower than 9.4% in the control ***:Milligan-Morgan hemorrhoidectomy with anal cushion suspension and partial internal sphincter resection is the optimal treatment for circumferential mixed hemorrhoids and can be widely applied in clinical settings.

关键词： Milligan-Morgan hemorrhoidectomy Mixed hemorrhoids Anal cushion Internal sphincter

Protective effect of human umbilical cord mesenchymal stem cell-derived exosomes on rat retinal neurons in hyperglycemia through the brain-derived neurotrophic factor/TrkB pathway

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International Journal of Ophthalmology(English edition) 2021年第11期14卷 1683-1689页

作者： Xiang Gao Guang-Hui He Xiao-Tian Zhang Song Chen College of Medicine Nankai UniversityTianjin 300071China Tianjin Eye hospital Tianjin 300020China Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300020China ophthalmic center of xinjiang production and construction corps hospital Urumqi 830002Xinjiang Uygur Autonomous RegionChina

AIM:To explore whether human umbilical cord mesenchymal stem cell(hUCMSC)-derived exosomes(hUCMSC-Exos)protect rat retinal neurons in high-glucose(HG)conditions by activating the brain-derived neurotrophic factor(BDNF)-Trk B ***:h UCMSC-Exos were collected with differential ultracentrifugation methods and observed by transmission electron ***-linked immunosorbent assays(ELISAs)was used to quantify BDNF in hUCMSC-Exos,and Western blot was used to identify surface markers of *** retinal neurons were divided into 4 ***,cell viability,cell apoptosis,and TrkB protein expression were measured in retinal ***:hUCMSCs and isolated hUCMSC-Exos were successfully *** hUCMSC-Exos showed a diameter of 30 to 150 nm and had a phospholipid bimolecular membrane structure,as observed by transmission electron *** showed the BDNF concentration of hUCMSCs-Exos was 2483.16±***-Exos effectively reduced the apoptosis of retinal neuron rate and improved neuron survival rate,meanwhile,the results of immunofluorescence verified the fluorescence intensity of TrKB in neurons *** all above effects were reduced by treated hUCMSCs-Exos with BDNF ***-Exos effectively reduced the apoptosis rate of retinal neurons by activating the BDNF-TrkB pathway in a HG ***:In the HG environment,hUCMSC-Exos could carry BDNF into rat retinal neurons,inhibiting neuronal apoptosis by activating the BDNF-TrkB pathway.

关键词： human umbilical cord mesenchymal stem cells exosomes diabetic retin opathy retinal n euro ns BDNF-TrkB pathway

Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy

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World Journal of Gastroenterology 2014年第26期20卷 8653-8659页

作者： Hong Ma Ayshamgul Hasim Batur Mamtimin Bin Kong Hai-Ping Zhang Ilyar Sheyhidin Department of Thoracic Surgery The First Affiliated HospitalMedical University of Xinjiang Department of Pathology College of Basic MedicineMedical University of Xinjiang centers of Pharmaceutical Analysis College of PharmacyMedical University of Xinjiang Pharmacy Department of production and construction corps General hospital of xinjiang

AIM: To perform plasma free amino acid (PFAA) profiling of esophageal squamous cell carcinoma (ESCC) patients at different pathological stages and healthy subjects.

关键词： Metabolomics High-performance liquid chromatography Esophageal squamous cell cancer Plasma Amino acids

Mesenchymal stem cell-derived exosomes inhibit the VEGF-A expression in human retinal vascular endothelial cells induced by high glucose

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International Journal of Ophthalmology(English edition) 2021年第12期14卷 1820-1827页

作者： Guang-Hui He Ying-Xue Ma Meng Dong Song Chen Yu-Chuan Wang Xiang Gao Bin Wu Jian Wang Jun-Hua Wang Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300070China Tianjin Eye hospital Tianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceTianjin 300020China ophthalmic center of xinjiang production and construction corps hospital Urumqi 830002Xinjiang Uygur Autonomous RegionChina Medical College of NanKai University Tianjin 300000China

AIM:To determine the effect of exosomes derived from human umbilical cord blood mesenchymal stem cells(hUCMSCs)on the expression of vascular endothelial growth factor A(VEGF-A)in human retinal vascular endothelial cells(HRECs).METHODS:Exosomes were isolated from hUCMSCs using cryogenic ultracentrifugation and characterized by transmission electron microscopy,Western blotting and nanoparticle tracking *** were randomly divided into a normal control group(group A),a high glucose model group(group B),a high glucose group with 25μg/mL(group C),50μg/mL(group D),and 100μg/mL exosomes(group E).Twenty-four hours after coculture,the cell proliferation rate was detected using flow cytometry,and the VEGF-A level was detected using *** coculture 8,16,and 24h,the expression levels of VEGF-A in each group were detected using PCR and Western ***:The characteristic morphology(membrane structured vesicles)and size(diameter between 50 and 200 nm)were observed under transmission electron *** average diameter of 122.7 nm was discovered by nanoparticle tracking analysis(NTA).The exosomal markers CD9,CD63,and HSP70 were strongly *** proliferation rate of the cells in group B increased after 24h of *** analyses revealed that the upregulation of VEGF-A expression in HRECs stimulated by high glucose could be downregulated by cocultured hUCMSC-derived exosomes(F=39.03,P<0.01).The upregulation of VEGF-A protein(group C:F=7.96;group D:F=17.29;group E:F=11.89;8h:F=9.45;16h:F=12.86;24h:F=42.28,P<0.05)and mRNA(group C:F=4.137;group D:F=13.64;group E:F=22.19;8h:F=7.253;16h:F=16.98;24h:F=22.62,P<0.05)in HRECs stimulated by high glucose was downregulated by cocultured hUCMSC-derived exosomes(P<0.05).CONCLUSION:hUCMSC-derived exosomes downregulate VEGF-A expression in HRECs stimulated by high glucose in time and concentration dependent manner.

关键词： mesenchymal stem cells exosomes retinal vascular endothelial cells vascular endothelial growth factor A coculture

Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging

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International Journal of Ophthalmology(English edition) 2021年第12期14卷 1828-1833页

作者： Yan Fu Xiang Gao Guang-Hui He Song Chen Zhao-Hui Gu Yue-Ling Zhang Li-Ying Li College of Medicine Nankai UniversityTianjin 300071China Department of Ophthalmology Baoding No.1 Central HospitalBaoding 071000Hebei ProvinceChina Tianjin Eye hospital Tianjin 300020China Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300020China ophthalmic center of xinjiang production and construction corps hospital Urumqi 830002Xinjiang Uygur Autonomous RegionChina

AIM:To assess the protective effect of human umbilical cord mesenchymal stem cell exosomes(hucMSC-Exs)in a diabetic rat model by using a variety of retinal ***:hucMSCs were subjected to differential ultracentrifugation for the collection of exosomes,and transmission electron microscopy(TEM),nanoparticle tracking analysis(NTA)using a NanoSight analysis system and Western blotting(WB)were used to analyze the expression of surface marker proteins such as CD63,CD9 and ***(STZ)was injected into the intraperitoneal cavity to establish a diabetic *** were divided into a normal group,diabetic group and hucMSC-Ex *** fluorescein angiography(FFA),optical coherence tomography(OCT)and other live imaging methods were used to observe the fundus of the ***,the eyeballs of rats from each group were collected for hematoxylin-eosin(HE)staining to further analyze the retinal ***:Through TEM,NTA and WB,we successfully isolated *** FFA and OCT confirmed that hucMSC-Exs effectively prevented early retinal vascular damage and thickening of the ***,HE staining of rat retinal sections revealed that exosomes effectively alleviated retinal structure disruption caused by ***:hucMSC-Exs have a protective effect on the retina in diabetic rat through FFA,OCT and HE staining.

关键词： human umbilical cord mesenchymal stem cells exosomes diabetic retinopathy fundus fluorescein angiography optical coherence tomography rat

Treatment of 30 Cases of Meniere＇s Disease with Acupuncture

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Journal of Acupuncture and Tuina Science 2010年第5期8卷 319-320页

作者：柏荣华 Acupuncture Department Zhujiang Road Branch of Xinjiang Production and Construction Corps Hospital Urumchi 830006 R R. China

Meniere＇s disease is a disorder of the inner ear pathologically characterized by hydrops of the membranous labyrinth and clinically manifested by repeated episodes of vertigo, sensorineural deafness and tinnitus. The... 详细信息

关键词： Acupuncture-moxibustion Therapy Direct Moxibustion Meniere Disease

Pneumocystis jirovecii diagnosed by next-generation sequencing of bronchoscopic alveolar lavage fluid: A case report and review of literature

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World Journal of Clinical Cases 2023年第4期11卷 866-873页

作者： Qing-Wei Cheng Hong-Li Shen Zhi-Hui Dong Qian-Qian Zhang Ya-Fen Wang Jin Yan Yu-Sheng Wang Ning-Gang Zhang Department of Oncology The Sixth Division HospitalXinjiang Production and Construction CorpsWujiaqu 831300Xinjiang Uygur Autonomous RegionsChina Department of Gastrointestinal Oncology Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Cancer HospitalChinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical UniversityTaiyuan 030013Shanxi ProvinceChina

BACKGROUND The advent of molecular targeted agents and immune checkpoint inhibitors has greatly improved the treatment of advanced renal cell carcinoma(RCC), thus significantly improving patient survival. The incidence of rare drug-related adverse events has gained increased *** SUMMARY We report a patient with advanced RCC treated with multiple lines of molecular targeted agents and immune checkpoint inhibitors, who developed a pulmonary infection after treatment with everolimus in combination with lenvatinib. Determining the pathogenic organism was difficult, but it was eventually identified as Pneumocystis jirovecii by next-generation sequencing(NGS) of bronchoscopic alveolar lavage fluid(BALF) and successfully treated with *** Rare pulmonary infections caused by molecular targeted agents are not uncommon in clinical practice, but their diagnosis is difficult. Evaluating BALF with NGS is a good method for rapid diagnosis of such infections.

关键词： Renal cell carcinoma Everolimus Pneumocystis jirovecii pneumonia Next-generation sequencing Bronchoscopic alveolar lavage fluid Case report

Melanin nanoparticles alleviate sepsis-induced myocardial injury by suppressing ferroptosis and inflammation

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Bioactive Materials 2023年第6期24卷 313-321页

作者： Chang Liu Quan Zou Huixin Tang Jia Liu Shiqi Zhang Caihong Fan Junwei Zhang Ruiqing Liu Yashan Liu Ruiyan Liu Yan Zhao Qiang Wu Zhi Qi Yanna Shen School of Medical Technology Tianjin Medical UniversityTianjin300203China Department of Molecular Pharmacology School of MedicineNankai UniversityTianjin300071China Key Laboratory of Emergency and Trauma of Ministry of Education Hainan Medical UniversityHaikou571199China Department of Radiology The Second Hospital of Tianjin Medical UniversityTianjin300211China Tianjin Key Laboratory of General Surgery in construction Tianjin Union Medical CenterTianjin300000China xinjiang production and construction corps hospital Xinjiang830092China

Myocardial injury as one of the severe complications leads to the increasing morbidity and mortality in patients with *** studies reported that reactive oxygen species(ROS)-mediated ferroptosis plays a critical role in the development of heart ***,we hypothesized that anti-ferroptosis agent might be a novel potential therapeutic strategy for sepsis-induced cardiac ***,we demonstrated that a small biocompatible and MRI-visible melanin nanoparticles(MMPP)improves myocardial function by inhibiting ROS-related ferroptosis signaling *** LPS-induced murine sepsis model,after a single dose intravenously injection of MMPP treatment,MMPP markedly alleviated the myocardial injury including cardiac function and heart structure disorder through suppressing iron-accumulation induced *** vitro,MMPP inhibited cardiomyocyte death by attenuating oxidative stress,inflammation and maintaining mitochondrial ***,our findings demonstrated that MMPP protected heart against sepsis-induced myocardial injury via inhibiting ferroptosis and inflammation,which might be a novel therapeutic approach in future.

关键词： Sepsis Myocardial injury Melanin nanoparticles Ferroptosis Inflammation