Protective effect of human umbilical cord mesenchymal stem cell-derived exosomes on rat retinal neurons in hyperglycemia through the brain-derived neurotrophic factor/TrkB pathway

作     者：Xiang Gao Guang-Hui He Xiao-Tian Zhang Song Chen 

作者机构：College of MedicineNankai UniversityTianjin 300071China Tianjin Eye HospitalTianjin 300020China Clinical College of OphthalmologyTianjin Medical UniversityTianjin 300020China Ophthalmic Center of Xinjiang Production and Construction Corps HospitalUrumqi 830002Xinjiang Uygur Autonomous RegionChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2021年第14卷第11期

页      面：1683-1689页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by Tianjin Science and Technology Project of China(No.14JCYBJC27400) Science and Technology Fund of Tianjin Eye Hospital(No.YKZD1901 No.YKYB1905) 

主　　题：human umbilical cord mesenchymal stem cells exosomes diabetic retin opathy retinal n euro ns BDNF-TrkB pathway 

摘      要：AIM:To explore whether human umbilical cord mesenchymal stem cell(hUCMSC)-derived exosomes(hUCMSC-Exos)protect rat retinal neurons in high-glucose(HG)conditions by activating the brain-derived neurotrophic factor(BDNF)-Trk B ***:h UCMSC-Exos were collected with differential ultracentrifugation methods and observed by transmission electron ***-linked immunosorbent assays(ELISAs)was used to quantify BDNF in hUCMSC-Exos,and Western blot was used to identify surface markers of *** retinal neurons were divided into 4 ***,cell viability,cell apoptosis,and TrkB protein expression were measured in retinal ***:hUCMSCs and isolated hUCMSC-Exos were successfully *** hUCMSC-Exos showed a diameter of 30 to 150 nm and had a phospholipid bimolecular membrane structure,as observed by transmission electron *** showed the BDNF concentration of hUCMSCs-Exos was 2483.16±***-Exos effectively reduced the apoptosis of retinal neuron rate and improved neuron survival rate,meanwhile,the results of immunofluorescence verified the fluorescence intensity of TrKB in neurons *** all above effects were reduced by treated hUCMSCs-Exos with BDNF ***-Exos effectively reduced the apoptosis rate of retinal neurons by activating the BDNF-TrkB pathway in a HG ***:In the HG environment,hUCMSC-Exos could carry BDNF into rat retinal neurons,inhibiting neuronal apoptosis by activating the BDNF-TrkB pathway.