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Journal of Integrative Agriculture 2014年第4期13卷 911-917页

作者： GUO Peng WANG Na LIU Yong-jie LU Cheng-ping College of veterinary Medicine Nanjing Agricultural University Institute of Animal quarantine Chinese Academy of Inspection and Quarantine

Aeromonas hydrophila isolates from clinical cases （n=43） were tested against 8 antimicrobial agents and typed by outer membrane protein （OMP） pattern by using sodium dodecyl sulfate gel electrophoresis. All isolates were resistant to ampicillin （MICs, ≥16 μg mL-1） and sulfamonomethoxine （MICs≥64 μg mLl）, but susceptible to norfloxacin （MICs,≤0.5 μg mL-1）. There was a high incidence of resistance to erythromycin （90.70%） and tylosin （93.02%）, while a low incidences of resistance to ciprofloxacin （2.33%）, enrofloxacin （2.33%） and florfenicol （4.65%）. Six different outer membrane protein patterns were found among 34 isolates by analyzing proteins in the range of 22 to 50 kDa, other than 9 isolates with their respective profiles. The strains with the similar OMP profiles had similar resistances. Compared with the other strains from the same OMP patterns, NB-1, *** and MR-1 had lacked the proteins in the range of 30 to 45 kDa and their resistance to florfenicol substantially increased. It is speculated that the outer membrane protein changes might correlate with decreased susceptibility to florfenicol in the three strains. Some strains which showed completely identical OMP types had a little difference in their resistance to fluoroquinolones, indicating that there might be other factors that were involved in the antimicrobial resistance of A. hydrophila.

关键词： Aeromonas hydrophila antimicrobial resistance outer membrane proteins

Metabolism of Mequindox in Isolated Rat Liver Cells

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Journal of Integrative Agriculture 2014年第1期13卷 158-166页

作者： LI Guang-hui SHAN Qi WANG Jing LI Ya-fei GAO Yan ZENG Zhen-ling national Reference Laboratory of veterinary Drug Residues (SCAU) College of Veterinary MedicineSouth China Agricultural University Jie Yang Entry-Exit Inspection and quarantine Bureau

Mequindox （MEQ）, 3-methyl-2-quinoxalinacetyl-l,4-dioxide, is widely used in Chinese veterinary medicine as an antimicrobial agent and feed additive. Its toxicity has been reported to be closely related to its metabolism. To understand the pathways underlying MEQ＇s metabolism more clearly, we studied its metabolism in isolated rat liver cells by using liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupole orbitrap （LC-LTQ-Orbitrap） mass spectrometry. The structures of MEQ metabolites and their product ions were readily and reliably characterized on the basis of accurate MS2 spectra and known structure of MEQ. Eleven metabolites were detected in isolated rat liver cells, two of which were detected for the first time in vitro. The major metabolic pathways reported previously for in vitro metabolism of MEQ in rat microsomes were confirmed in this study, including N O group reduction, carbonyl reduction, and methyl monohydroxylation. In addition, we fotmd that acetyl hydroxylation was an important pathway of MEQ metabolism. The results also demonstrate that cellular systems more closely simulate in vivo conditions than do other in vitro systems such as microsomes. Taken together, these data contribute to our understanding of the in vivo metabolism of MEQ.

关键词： mequindox isolated rat liver cells metabolism metabolites LC-LTQ-Orbitrap

Identification and Functional Analysis of Differentially Expressed Genes of Ascaris suum Goeze,1782 from Ascaris lumbricoides Linnaeus, 1758

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Agricultural Sciences in China 2010年第6期9卷 903-910页

作者： WU Shao-qiang LIN Xiang-mei ZHU Xing-quan Institute of Animal and Plant quarantine Chinese Academy of Inspection and Quarantine Beijing 100029 P.R.China State Key Laboratory of veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 P.R. China

In order to provide further evidence to prove that Ascaris suum Goeze,1782 and Ascaris lumbricoides Linnaeus,1758 are really different species in taxonomy,and to identity *** larval migrans-related genes for diagnosis and prevention use,*** genes that were differentialy expressed from the same gender of *** were enriched by subtracting the same expressed genes using suppression subtractive hybridization （SSH） *** of the selectively enriched cDNA was verified by Southern blot *** female *** specific cDNA library was then constructed and *** local alignment search tool （BLAST） analysis of female *** specific cDNA identified 6 specific ESTs with tentative functions related to larva *** study provided further evidence for differentiating *** from *** for the detailed information and application of the 6 ESTs are worth being done in the future studies.

关键词： Ascaris suum Ascaris lumbricoides nematode suppression subtractive hybridization EST larva migrans

Genetic diversity of avian infectious bronchitis isolates in Korea

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Genetic diversity of avian infectious bronchitis isolates in...

Molecular Characterization of <i>Listeria monocytogenes</i>Based on the PFGE and RAPD in Korea

The 15th World veterinary Poultry Congress

作者： Eun-kyoung Lee Woo-jin Jeon Youn-jeong Lee Ok-mi Jeong Jun-gu Choi Yong-joo Kim Yong-kuk Kwon Jun-hun Kwon Kang-seuk Choi national veterinary research and quarantine service/Anyang South Korea

Introduction Infectious bronchitis(IB)is an acute,highly contagious disease of the poultry characterized by respiratory signs,nephritis,and reduced egg production in laying *** is still end

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Advances in Microbiology 2012年第4期2卷 605-616页

作者： Sanghun Park Jihun Jung Sungsun Choi Younghee Oh Jibho Lee Heesun Chae Seunghee Ryu Hyowon Jung Gunyong Park Sungmin Choi Bogsoon Kim Junghun Kim Young Zoo Chae Byungyeol Jung Myunghun Lee Hyunsoo Kim Animal Disease Diagonsis Division National Veterinary Research and Quarantine Service Anyang City Korea College of veterinary Medcine Chungnam National University Daejeon Korea Seoul Metropolitan Government research Institute of Public Health and Environment Seoul Korea veterinary Pharmacology Division National Veterinary Research and Quarantine Service Anyang City Korea

This study was performed to characterize 35 L. monocytogenes isolates from animals, foods, environmental samples collected between 1997 and 2007 with no apparent epidemiological relations, and five reference isolates using serotypic, genotypic and molecular typing methods to understand the pattern of strain distribution in Korea. For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. We also compared RAPD and PFGE to determine genetic characterization of L. monocytogenes strains to define the genetic diversity. Serotype patterns of the 30 L. monocytogenes strains was as follows;9 isolates (30.0%) belonged to serotype, 7 isolates (23.3%) belonged to serotype 4b, 4 isolates (13.3%) belonged to serotype 1/2b, 3 isolates (10.0%) belonged to serotype 1/2c, 2 (6.7%) isolates belonged to4c, 2 (6.7%) isolates belonged to NT (Non Type), one isolate (3.2%) belonged to3aand 3b, and4a, respectively. Although, a limited number of isolates were analyzed in this study, molecular typing with RAPD and PFGE indicated that PFGE is more discriminatory for the subtyping L. monocytogenes than RAPD. Some L. monocytogenes isolates by RAPD and PFGE types are associated with specific sources. And, combining data obtained by these methods will increases the likelihood of strain discrimination.

关键词： Listeria monocytogenes PFGE RAPD

Efficacy in chickens of commercially inactivated Newcastle disease virus vaccines against recent Korean epizootic strain of NDV

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Efficacy in chickens of commercially inactivated Newcastle d...

Enhancing the stability and ecological safety of mass-reared transgenic strains for field release by redundant conditional lethality systems

The 15th World veterinary Poultry Congress

作者： Woo-jin Jeon Eun-kyoung Lee Eun-im Yun Youn-jeong Lee Ok-mi Jeong Yong-joo Kim Jun-hun Kwon Kang-seuk Choi national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea national veterinary research ＆ quarantine service 480 Anyang-6AnyangGyeonggi 430-824Korea

＜正＞Introduction Newcastle disease（ND）is one of the most disastrous diseases in the poultry *** Korea, extensive vaccination against ND has been implemented,but outbreaks have not stopped ***, there are at least e...

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Insect Science 2016年第2期23卷 225-234页

作者： Alfred M. Handler USDA Agricultural Research Service Center for Medical Agricultural and Veterinary Entomology Gainesville FL 32608 USA

The genetic manipulation of agriculturally important insects now allows the development of genetic sexing and male sterility systems for more highly efficient biologically-based population control programs, most notably the Sterile Insect Technique （SIT）, for both plant and animal insect pests. Tetracycline-suppressible （Tet-off） conditional lethal systems may function together so that transgenic strains will be viable and fertile on a tetracycline-containing diet, but female-lethal and male sterile in tetracycline-free condi- tions. This would allow their most efficacious use in a unified system for sterile male-only production for SIT. A critical consideration for the field release of such transgenic insect strains, however, is a determination of the frequency and genetic basis of lethality revertant survival. This will provide knowledge essential to evaluating the genetic stability of the lethality system, its environmental safety, and provide the basis for modifications ensur- ing optimal efficacy. For Tet-off lethal survival determinations, development of large-scale screening protocols should also allow the testing of these modifications, and test the ability of other conditional lethal systems to fully suppress propagation of rare Tet-off survivors. If a dominant temperature sensitive （DTS） pupal lethality system proves efficient for sec- ondary lethality in Drosophila, it may provide the safeguard needed to support the release of sexing/sterility strains, and potentially, the release of unisex lethality strains as a form of genetic male sterility. Should the DTS Prosβ21 mutation prove effective for redundant lethality, its high level of structural and functional conservation should allow host-specific cognates to be created for a wide range of insect species.

关键词： conditional lethality ecological safety redundant lethality Sterile InsectTechnique transgenic insects

韩国入境口岸进口植物上截获的盾蚧(半翅目:盾蚧总科)(英文)

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昆虫学报 2009年第9期52卷 1039-1054页

作者： Soo-Jung SUH Jungyoun JI Central Post-entry quarantine Station National Plant Quarantine Service Jungbu Regional Office National Plant Quarantine Service

1996-2008年在韩国入境口岸进口植物上截获了发生于13个国家的盾蚧类昆虫总共51种。本文提供了这些种类的检索表及照片,可供分类鉴定参考。

关键词：盾蚧分类鉴定检疫截获物种进口植物韩国

Induction of Apoptosis in the Immature Mouse Testes by a Mixture of Melamine and Cyanuric Acid

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Journal of Integrative Agriculture 2012年第12期11卷 2058-2066页

作者： YOU Hua ZHU Jin-feng SHE Rui-ping CHANG Ling-ling SHI Rui-han DING Ye CHI Li-juan LIU Bin YUE Zhuo TIAN Ji-jing MAO Jing-jing SU Li-fang Key Laboratory of Zoonosis Ministry of Agriculture/Department of Veterinary Pathology College of Veterinary Medicine China Agricultural University China Animal Disease Control Center national Animal Husbandry service

The toxicity of melamine has attracted much attention since the recent outbreaks of renal injury in pets and infants. Previous studies indicated that melamine by itself had low toxicity, whereas a mixture of melamine and cyanuric acid （M＋CA） could cause serious renal damage. At present, most researches on the toxicity of M＋CA are focused on the kidney. However, little is known about the adverse effects of this mixture on the reproductive system. In the present study, the toxicity of M＋CA to testes was investigated. Immature male mice were orally dosed with 0, 0.6, 3, and 15 mg kg-1 d-1 of a 1：1 M＋CA for 28 d. Pathological changes occurred in germ cells, such as loose arrangement, reduced numbers and karyopyknosis, indicating that this mixture was toxic to spermatogenesis. Compared with the control group, the TUNEL- positive germ cells increased significantly and the ratio of Bcl-2 to Bax, total antioxidant capacity and superoxide dismutase activity decreased significantly in the 3 and 15 mg kg-1 d- M＋CA treated group, while the activities of caspase-3, caspase- 8 and caspase-9 remained unchanged. The results suggest that M＋CA can induce apoptosis in the mice testes. The downregulation of Bcl-2/Bax and oxidative stress may play a pivotal role in the induction of apoptosis by M＋CA in mice testes.

关键词： melamine/cyanuric acid mixture testes injury caspase-independent apoptosis

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韩国传染性支气管炎分离株的遗传多样性

韩国传染性支气管炎分离株的遗传多样性