Enhancing the stability and ecological safety of mass-reared transgenic strains for field release by redundant conditional lethality systems

作     者：Alfred M. Handler 

作者机构：USDA Agricultural Research Service Center for Medical Agricultural and Veterinary Entomology Gainesville FL 32608 USA 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2016年第23卷第2期

页      面：225-234页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081803[工学-地质工程] 08[工学] 0818[工学-地质资源与地质工程] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：the Co-ordinated Research Project the Biotechnology Risk Assessment Grant Program the USDA National Institute of Food and Agriculture 

主　　题：conditional lethality ecological safety redundant lethality Sterile InsectTechnique transgenic insects 

摘      要：The genetic manipulation of agriculturally important insects now allows the development of genetic sexing and male sterility systems for more highly efficient biologically-based population control programs, most notably the Sterile Insect Technique （SIT）, for both plant and animal insect pests. Tetracycline-suppressible （Tet-off） conditional lethal systems may function together so that transgenic strains will be viable and fertile on a tetracycline-containing diet, but female-lethal and male sterile in tetracycline-free condi- tions. This would allow their most efficacious use in a unified system for sterile male-only production for SIT. A critical consideration for the field release of such transgenic insect strains, however, is a determination of the frequency and genetic basis of lethality revertant survival. This will provide knowledge essential to evaluating the genetic stability of the lethality system, its environmental safety, and provide the basis for modifications ensur- ing optimal efficacy. For Tet-off lethal survival determinations, development of large-scale screening protocols should also allow the testing of these modifications, and test the ability of other conditional lethal systems to fully suppress propagation of rare Tet-off survivors. If a dominant temperature sensitive （DTS） pupal lethality system proves efficient for sec- ondary lethality in Drosophila, it may provide the safeguard needed to support the release of sexing/sterility strains, and potentially, the release of unisex lethality strains as a form of genetic male sterility. Should the DTS Prosβ21 mutation prove effective for redundant lethality, its high level of structural and functional conservation should allow host-specific cognates to be created for a wide range of insect species.