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Virologica Sinica 2024年第2期39卷 351-354页

作者： Xiangju Wu Lei Chen Chao Sui Yue Hu Dandan Jiang Fan Yang Laura C.Miller Juntong Li Xiaoyan Cong Nataliia Hrabchenko Changhee Lee Yijun Du Jing Qi Shandong Key Laboratory of Animal Disease Control and Breeding Key Laboratory of Livestock and Poultry Multi-omics of MARAInstitute of Animal Science and Veterinary MedicineInstitute of Crop Germplasm ResourcesShandong Academy of Agricultural SciencesJinan250100China College of Life Science Shandong Normal UniversityJinan250358China State Key Laboratory of veterinary Etiological Biology National Foot and Mouth Disease Reference LaboratoryKey Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730050China Department of Diagnostic medicine and Pathobiology College of Veterinary MedicineKansas State UniversityManhattanKS66506USA College of veterinary medicine and Virus Vaccine research center Gyeongsang National UniversityJinju52828Republic of Korea

Due to our negligence,the original version of this article,published online on Mar 14,2023,contained some mistakes in several Figs.In Fig.2D,the positions of the bands for protein 3A and 3B were incorrectly shifted.Th... 详细信息

关键词： STAT1 FMDV bands

Epigenetic mechanisms involved in the neuroprotective effect of scorpion extract in a Parkinson's disease murine model based on multi-omics approach

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Journal of Traditional Chinese medicine 2021年第3期41卷 390-396页

作者： Joong Sun Kim Hye-Sun Lim Byeong Cheol Moon Mary Jasmin Ang Sung-Ho Kim Changjong Moon Boseok Seong Yunji Jang Hyung-Yong Kim Chul Kim Herbal medicine Resources research center Korea Institute of Oriental MedicineJeollanamdo 58245Republic of Korea College of veterinary medicine and BK21 Plus Project Team Chonnam National UniversityGwangju 61186Republic of Korea Future medicine Division Korea Institute of Oriental MedicineDaejeon 34054Republic of Korea research and Development center Insilicogen Inc.Yonginsi 16954Republic of Korea

OBJECTIVE: To investigate whether scorpion extract elicits a neuroprotective effect in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-treated mice models, and the genes associated with the therapeutic effects using RNA sequencing(seq)analysis.METHODS: This study investigated the changes in interaction between messenger ribonucleic acid(m RNA) expression and deoxyribonucleic acid(DNA) methylation related to the protective effects of scorpion extracts, in the substantia nigra(SN)region of a MPTP-induced Parkinson's disease(PD)model.RESULTS: In this model, scorpion extracts attenuated the motor impairment as demonstrated by the rotarod and open field tests. Scorpion extracts consistently attenuated the decrease of tyrosine hydroxylase(TH) positive neural cells in the SN and striatum of mice. We profiled genomewide DNA methylation using Methyl-Seq and measured the transcriptome using RNA-Seq in murine SN in the following groups: vehicle-treated MPTP-induced PD mice and scorpion extracttreated MPTP-induced PD mice. In total, 13 479 differentially expressed genes were identified in association with the anti-PD effect of the scorpion extract, mainly in the promoter and coding regions.Among them, 47 were negatively correlated downregulated genes. Nineteen genes out of 47 downregulated genes were negatively correlated with the expression of the other 28 genes. Among these genes, SGSM1 was related to dopaminergic neurons including dopamine transporters, TH, dihydroxyphenylalanine decarboxylase, and dopamine D2 receptor.CONCLUSION: This study provides insights into the anti-parkinsonian effects of scorpion extract and reveals the epigenetic targets in its therapeutic mechanism.

关键词： scorpions 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine Parkinson disease DNA methylation

Genome degradation promotes Salmonella pathoadaptation by remodeling fimbriae-mediated proinflammatory response

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national Science Review 2023年第10期10卷 32-43页

作者： Xiao Zhou Xiamei Kang Jiaqi Chen Yan Song Chenghao Jia Lin Teng Yanting Tang Zhijie Jiang Xianqi Peng Xiaoxi Tao Yiwei Xu Linlin Huang Xuebin Xu Yaohui Xu Tengfei Zhang Shenye Yu Jiansen Gong Shaohui Wang Yuqing Liu Guoqiang Zhu Corinna Kehrenberg Fran?ois-Xavier Weill Paul Barrow Yan Li Guoping Zhao Min Yue Shandong Key Laboratory of Animal Disease Control and Breeding Institute of Animal Science and Veterinary MedicineShandong Academy of Agricultural Sciences College of veterinary medicine Yangzhou University Institute for veterinary Food Science Faculty of Veterinary MedicineJustus-Liebig University Giessen Institut Pasteur Université Paris Cité Unité des bactéries pathogènes entériques School of veterinary medicine University of Surrey School of Life Science Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences CAS Key Laboratory of Synthetic Biology Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences Department of Microbiology and Microbial Engineering School of Life SciencesFudan University State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesNational Medical Center for Infectious DiseasesThe First Affiliated Hospital College of Medicine Zhejiang University Zhejiang Provincial Key Laboratory of Preventive veterinary medicine Institute of Preventive veterinary Sciences and Department of veterinary medicine Zhejiang University College of Animal Sciences Ningbo Academy of Agricultural Sciences Hainan Institute of Zhejiang University Department of Microbiology Laboratory Shanghai Municipal Center for Disease Control and Prevention College of veterinary medicine Henan University of Animal Husbandry and Economy Key Laboratory of Prevention and Control Agents for Animal Bacteriosis Institute of Animal Husbandry and Veterinary Hubei Academy of Agricultural Sciences Division of Bacterial Diseases State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural Sciences Poultry Institute Chinese Academy of Agricultural Sciences Department of Animal Public Health Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences

Understanding changes in pathogen behavior(e.***.increased virulence,a shift in transmission channel) is critical for the public health management of emerging infectious diseases.Genome degradation via gene depletion or inactivation is recognized as a pathoadaptive feature of the pathogen evolving with the host.However,little is known about the exact role of genome degradation in affecting pathogenic behavior,and the underlying molecular detail has yet to be examined.Using large-scale global avian-restricted Salmonella genomes spanning more than a century,we projected the genetic diversity of Salmonella Pullorum(bvSP)by showing increasingly antimicrobial-resistant ST92 prevalent in Chinese flocks.The phylogenomic analysis identified three lineages in bvSP,with an enhancement of virulence in the two recently emerged lineages(L2/L3),as evidenced in chicken and embryo infection assays.Notably,the ancestor L1 lineage resembles the Salmonella serovars with higher metabolic flexibilities and more robust environmental tolerance,indicating stepwise evolutionary trajectories towards avian-restricted lineages.Pan-genome analysis pinpointed fimbrial degradation from a virulent lineage.The later engineeredfim-deletion mutant,and all other five fimbrial systems,revealed behavior switching that restricted horizontal fecal-oral transmission but boosted virulence in chicks.By depleting fimbrial appendages,bvSP established persistent replication with less proinflammation in chick macrophages and adopted vertical transovarial transmission,accompanied by ever-increasing intensification in the poultry industry.Together,we uncovered a previously unseen paradigm for remodeling bacterial surface appendages that supplements virulence-enhanced evolution with increased vertical transmission.

关键词： Salmonella host adaptation pathogenic evolution virulence evolution vertical transmission fimbrial adhesin

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry

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Science China(Life Sciences) 2022年第2期65卷 341-361页

作者： Guoli Li Bingqian Su Pengfei Fu Yilin Bai Guangxu Ding Dahua Li Jiang Wang Guoyu Yang Beibei Chu College of veterinary medicine Henan Agricultural UniversityZhengzhou 450046China Key Laboratory of Animal Biochemistry and Nutrition Ministry of Agriculture and Rural Affairs of the People’s Republic of ChinaZhengzhou 450046China International Joint research center of national Animal Immunology Henan Agricultural UniversityZhengzhou 450046China College of veterinary medicine Northwest A&F UniversityYangling 712100China

Viruses utilize cellular lipids and manipulate host lipid metabolism to ensure their replication and spread.Therefore,the identification of lipids and metabolic pathways that are suitable targets for antiviral development is crucial.Using a library of compounds targeting host lipid metabolic factors and testing them for their ability to block pseudorabies virus(PRV)and vesicular stomatitis virus(VSV)infection,we found that U18666A,a specific inhibitor of Niemann-Pick C1(NPC1),is highly potent in suppressing the entry of diverse viruses including pseudotyped severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).NPC1 deficiency markedly attenuates viral growth by decreasing cholesterol abundance in the plasma mem-brane,thereby inhibiting the dynamics of clathrin-coated pits(CCPs),which are indispensable for clathrin-mediated endocytosis.Significantly,exogenous cholesterol can complement the dynamics of CCPs,leading to efficient viral entry and infectivity.Administration of U18666A improves the survival and pathology of PRV-and influenza A virus-infected mice.Thus,our studies demonstrate a unique mechanism by which NPC1 inhibition achieves broad antiviral activity,indicating a potential new therapeutic strategy against SARS-CoV-2,as well as other emerging viruses.

关键词： broad-spectrum antiviral NPC inhibition CCP dynamics viral entry

Responsive changes of rumen microbiome and metabolome in dairy cows with different susceptibility to subacute ruminal acidosis

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Animal Nutrition 2022年第1期8卷 331-340页

作者： Tao Zhang Yingyu Mu Ruiyang Zhang Yanfeng Xue Changzheng Guo Wangpan Qi Jiyou Zhang Shengyong Mao national center for International research on Animal Gut Nutrition National Experimental Teaching Demonstration Center of Animal ScienceCentre for Ruminant Nutrition and Feed Technology ResearchCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing210095China College of Animal Science and veterinary medicine Shenyang Agricultural UniversityShenyang110866China College of Animal Science and Technology Shihezi UniversityShihezi832000China

Subacute ruminal acidosis(SARA)represents one of the most important digestive disorders in intensive dairy farms,and dairy cows are individually different in the severity of SARA risk.The objectives of the current study were to investigate differences in the ruminal bacterial community and metabolome in dairy cattle with different susceptibility to SARA.In the present study,12 cows were initially enrolled in the experiment.Based on average ruminal pH,4 cows with the lowest ruminal pH were assigned to the susceptible group(SUS,pH=5.76,n=4)and 4 cows with the highest ruminal pH assigned to the tolerant group(TOL,pH=6.10,n=4).Rumen contents from susceptible(SUS,n=4)and tolerant(TOL,n=4)dairy cows were collected through rumen fistula to systematically reveal the rumen microbial and metabolic alterations of dairy cows with different susceptibility to SARA using multi-omics approaches(16S and 18S rRNA gene sequencing and metabolome).The results showed that despite being fed the same diet,SUS cows had lower ruminal pH and higher concentrations of total volatile fatty acids(VFA)and propionate than TOL cows(P0.05).The principal coordinates analysis based on the analysis of molecular variance indicated a significant difference in bacterial composition between the two groups(P=0.01).More specifically,the relative abundance of starch-degrading bacteria(Prevotella spp.)was greater(P0.05).Metabolomics analysis revealed that the concentrations of organic acids(such as lactic acid),biogenic amines(such as histamine),and bacterial degradation prod

关键词： Subacute ruminal acidosis Microbiome Metabolome Dairy cows

Ameliorative effects of human adipose tissue-derived mesenchymal stem cells on myelin basic protein-induced experimental autoimmune encephalomyelitis in Lewis rats

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Neural Regeneration research 2011年第16期6卷 1205-1210页

作者： Myung-Soon Ko Hyeong-geun Park Young-Min Yun Jeong Chan Ra Taekyun Shin Kyoung-Kap Lee College of veterinary medicine Jeju National University Stem Cell research center RNL BIO Co.Ltd.

Mesenchymal stem cells have been previously shown to exert an immunomodulatory function. The present study sought to investigate the effects of multipotential human adipose tissue-derived mesenchymal stem cells （hAdMSCs） on disease progression and cytokine expression in Lewis rats with experimental autoimmune encephalomyelitis （EAE） induced by myelin basic protein. The duration of EAE paralysis in the group treated on day 7 posfimmunization with 5 × 10^6 hAdMSCs was significantly reduced compared with the vehicle-treated controls and the 1 x 106 hAdMSC- treated group. The duration of EAE paralysis in the groups treated with 5 × 10^6 hAdMSCs on both day 1 and day 7 postimmunization was significantly reduced compared with the vehicle-treated controls and the groups treated with 5 × 10^6 hAdMSCs on both day 7 and day 10 postimmunization. The mRNA expression of interleukin-10 and indoleamine 2, 3-dioxygenase was significantly decreased in the hAdMSC-treated group compared with the vehicle-treated group. These findings suggest that the ameliorative effects of hAdMSCs on EAE symptoms operate in a dose- and time-dependent manner and can be mediated in part by the ample production of anti-inflammatory cytokines.

关键词： experimental autoimmune encephalomyelitis human adipose tissue mesenchymal stem cells intedeukin-10 interferon-gamma indoleamine 2, 3-dioxygenase neural regeneration

Viral vectored vaccines:design,development,preventive and therapeutic applications in human diseases

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Signal Transduction and Targeted Therapy 2023年第5期8卷 2113-2150页

作者： Shen Wang Bo Liang Weiqi Wang Ling Li Na Feng Yongkun Zhao Tiecheng Wang Feihu Yan Songtao Yang Xianzhu Xia Key Laboratory of Jilin Province for Zoonosis Prevention and Control Changchun Veterinary Research InstituteChinese Academy of Agricultural SciencesChangchunChina College of veterinary medicine Jilin UniversityChangchunChina China national research center for Exotic Animal Diseases China Animal Health and Epidemiology CenterQingdaoChina

Human diseases,particularly infectious diseases and cancers,pose unprecedented challenges to public health security and the global economy.The development and distribution of novel prophylactic and therapeutic vaccines are the prioritized countermeasures of human disease.Among all vaccine platforms,viral vector vaccines offer distinguished advantages and represent prominent choices for pathogens that have hampered control efforts based on conventional vaccine approaches.Currently,viral vector vaccines remain one of the best strategies for induction of robust humoral and cellular immunity against human diseases.Numerous viruses of different families and origins,including vesicular stomatitis virus,rabies virus,parainfluenza virus,measles virus,Newcastle disease virus,influenza virus,adenovirus and poxvirus,are deemed to be prominent viral vectors that differ in structural characteristics,design strategy,antigen presentation capability,immunogenicity and protective efficacy.This review summarized the overall profile of the design strategies,progress in advance and steps taken to address barriers to the deployment of these viral vector vaccines,simultaneously highlighting their potential for mucosal delivery,therapeutic application in cancer as well as other key aspects concerning the rational application of these viral vector vaccines.Appropriate and accurate technological advances in viral vector vaccines would consolidate their position as a leading approach to accelerate breakthroughs in novel vaccines and facilitate a rapid response to public health emergencies.

关键词： vaccines diseases protective

3C^(pro)of FMDV inhibits type II interferon-stimulated JAK-STAT signaling pathway by blocking STAT1 nuclear translocation

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Virologica Sinica 2023年第3期38卷 387-397页

作者： Xiangju Wu Lei Chen Chao Sui Yue Hu Dandan Jiang Fan Yang Laura C.Miller Juntong Li Xiaoyan Cong Nataliia Hrabchenko Changhee Lee Yijun Du Jing Qi Shandong Key Laboratory of Animal Disease Control and Breeding/Key Laboratory of Livestock and Poultry Multi-omics of MARA Institute of Animal Science and Veterinary MedicineInstitute of Crop Germplasm ResourcesShandong Academy of Agricultural SciencesJinan250100China College of Life Science Shandong Normal UniversityJinan250358China State Key Laboratory of veterinary Etiological Biology/national Foot and Mouth Disease Reference Laboratory/Key Laboratory of Animal Virology of Ministry of Agriculture Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730050China Department of Diagnostic medicine and Pathobiology College of Veterinary MedicineKansas State UniversityManhattanKS66506USA College of veterinary medicine and Virus Vaccine research center Gyeongsang National UniversityJinju52828Republic of Korea

Foot-and-mouth disease virus(FMDV)has developed various strategies to antagonize the host innate immunity.FMDV Lpro and 3Cpro interfere with type I IFNs through different mechanisms.The structural protein VP3 of FMDV degrades Janus kinase 1 to suppress IFN-γsignaling transduction.Whether non-structural proteins of FMDV are involved in restraining type II IFN signaling pathways is unknown.In this study,it was shown that FMDV replication was resistant to IFN-γtreatment after the infection was established and FMDV inhibited type II IFN induced expression of IFN-γ-stimulated genes(ISGs).We also showed for the first time that FMDV non-structural protein 3C antagonized IFN-γ-stimulated JAK-STAT signaling pathway by blocking STAT1 nuclear translocation.3C^(pro)expression significantly reduced the ISGs transcript levels and palindromic gamma-activated sequences(GAS)promoter activity,without affecting the protein level,tyrosine phosphorylation,and homodimerization of STAT1.Finally,we provided evidence that 3C protease activity played an essential role in degrading KPNA1 and thus inhibited ISGs mRNA and GAS promoter activities.Our results reveal a novel mechanism by which an FMDV non-structural protein antagonizes host type II IFN signaling.

关键词： Foot-and-mouth disease virus(FMDV) 3C IFN-γ JAK-STAT signaling pathway STAT1 KPNA1

Equisetin is an anti-obesity candidate through targeting 11β-HSD1

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2358-2373页

作者： Zhenlu Xu Dongyun Liu Dong Liu Xue Ren Haibo Liu Guihong Qi Yue Zhou Chongming Wu Kui Zhu Zhongmei Zou Jing Yuan Wenhan Lin Peng Guo Pharmacology and Toxicology research center Institute of Medicinal Plant DevelopmentChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100193China Capital Institute of Pediatrics Beijing 100020China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China national center for veterinary Drug Safety Evaluation College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China

Obesity is increasingly prevalent globally, searching for therapeutic agents acting on adipose tissue is of great importance. Equisetin(EQST), a meroterpenoid isolated from a marine sponge-derived fungus, has been reported to display antibacterial and antiviral activities. Here, we revealed that EQST displayed anti-obesity effects acting on adipose tissue through inhibiting adipogenesis in vitro and attenuating HFD-induced obesity in mice, doing so without affecting food intake, blood pressure or heart rate.We demonstrated that EQST inhibited the enzyme activity of 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), a therapeutic target of obesity in adipose tissue. Anti-obesity properties of EQST were all offset by applying excessive 11β-HSD1’s substrates and 11β-HSD1 inhibition through knockdown in vitro or 11β-HSD1 knockout in vivo. In the 11β-HSD1 bypass model constructed by adding excess11β-HSD1 products, EQST’s anti-obesity effects disappeared. Furthermore, EQST directly bond to11β-HSD1 protein and presented remarkable better intensity on 11β-HSD1 inhibition and better efficacy on anti-obesity than known 11β-HSD1 inhibitor. Therefore, EQST can be developed into anti-obesity candidate compound, and this study may provide more clues for developing higher effective 11β-HSD1 inhibitors.

关键词： EQST Obesity Adipocyte Lipid accumulation Preadipocyte differentiation Adipose tissue 11β-HSD1 11β-HSD1 inhibitor

The neuroprotective effects of human growth hormone as a potential treatment for amyotrophic lateral sclerosis

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Neural Regeneration research 2015年第8期10卷 1201-1203页

作者： Jin-Young Chung Jun-Sang Sunwoo Min-Wook Kim Manho Kim Department of veterinary Internal medicine and Geriatrics Kangwon National University Department of Neurology Seoul National University Hospital Department of Rehabilitation medicine College of Medicine The Catholic University of Korea Institute of Catholic Integrative medicine (ICIM) Incheon St. Mary’s Hospital Protein Metabolism Medical research center Seoul National University College of Medicine

Human growth hormone（GH）is a single-chain polypeptide of 191 amino acids that is involved in the regulation of various physiological processes,such as growth and metabolism.GH is synthesized and secreted by somatotropic cells in the anterior pituitary gland.Its secretion is primarily regulated by a balance between growth hormone-releasing hormone and growth hormone-inhibiting hormone,

关键词： amyotrophic metabolism secretion physiological inhibiting secreted neuronal primarily pituitary polypeptide