Epigenetic mechanisms involved in the neuroprotective effect of scorpion extract in a Parkinson's disease murine model based on multi-omics approach

作     者：Joong Sun Kim Hye-Sun Lim Byeong Cheol Moon Mary Jasmin Ang Sung-Ho Kim Changjong Moon Boseok Seong Yunji Jang Hyung-Yong Kim Chul Kim Joong Sun Kim;Hye-Sun Lim;Byeong Cheol Moon;Mary Jasmin Ang;Sung-Ho Kim;Changjong Moon;Boseok Seong;Yunji Jang;Hyung-Yong Kim;Chul Kim

作者机构：Herbal Medicine Resources Research CenterKorea Institute of Oriental MedicineJeollanamdo 58245Republic of Korea College of Veterinary Medicine and BK21 Plus Project TeamChonnam National UniversityGwangju 61186Republic of Korea Future Medicine DivisionKorea Institute of Oriental MedicineDaejeon 34054Republic of Korea Research and Development CenterInsilicogen Inc.Yonginsi 16954Republic of Korea 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2021年第41卷第3期

页      面：390-396页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Korea Institute of Oriental Medicine:the Verification of Efficacy and Safety for Chung-bu Medicinal Materials Described in the Dong Ui Bo Gam (K18411) Applicational Development of Standardized Herbal Resources (KSN1911420) 

主　　题：scorpions 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine Parkinson disease DNA methylation 

摘      要：OBJECTIVE: To investigate whether scorpion extract elicits a neuroprotective effect in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-treated mice models, and the genes associated with the therapeutic effects using RNA sequencing(seq)***: This study investigated the changes in interaction between messenger ribonucleic acid(m RNA) expression and deoxyribonucleic acid(DNA) methylation related to the protective effects of scorpion extracts, in the substantia nigra(SN)region of a MPTP-induced Parkinson s disease(PD)***: In this model, scorpion extracts attenuated the motor impairment as demonstrated by the rotarod and open field tests. Scorpion extracts consistently attenuated the decrease of tyrosine hydroxylase(TH) positive neural cells in the SN and striatum of mice. We profiled genomewide DNA methylation using Methyl-Seq and measured the transcriptome using RNA-Seq in murine SN in the following groups: vehicle-treated MPTP-induced PD mice and scorpion extracttreated MPTP-induced PD mice. In total, 13 479 differentially expressed genes were identified in association with the anti-PD effect of the scorpion extract, mainly in the promoter and coding *** them, 47 were negatively correlated downregulated genes. Nineteen genes out of 47 downregulated genes were negatively correlated with the expression of the other 28 genes. Among these genes, SGSM1 was related to dopaminergic neurons including dopamine transporters, TH, dihydroxyphenylalanine decarboxylase, and dopamine D2 ***: This study provides insights into the anti-parkinsonian effects of scorpion extract and reveals the epigenetic targets in its therapeutic mechanism.