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Genetically modified pigs with CD163 point mutation are resistant to HP-PRRSV infection

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Zoological research 2024年第4期45卷 833-844页

作者： Ying Liu Lin Yang Hong-Yong Xiang Ming Niu Jia-Cheng Deng Xue-Yuan Li Wen-Jie Hao Hong-Sheng Ou-Yang Tong-Yu Liu Xiao-Chun Tang Da-Xin Pang Hong-Ming Yuan State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Animal Genome Editing Technology Innovation CenterCollege of Animal SciencesJilin UniversityChangchunJilin 130062China Chifeng Best genetics technology Co. Ltd.ChifengNei Mongol 024000China Chongqing Jitang Biotechnology research Institute Co. Ltd.Chongqing 400015China

Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine *** the CD163 SRCR5 domain,either through deletion or substitution,can eff1ectively confer resistance to PRRSV infection in ***,large fragment modifications in pigs inevitably raise concerns about potential adverse effects on growth *** the impact of genetic modifications on normal physiological functions is a promising direction for developing PRRSV-resistant *** the current study,we identified a specific functional amino acid in CD163 that influences PRRSV *** infection experiments conducted on Marc145 and PK-15CD163 cells illustrated that the mE535G or corresponding pE529G mutations markedly inhibited highly pathogenic PRRSV(HP-PRRSV)proliferation by preventing viral binding and ***,individual viral challenge tests revealed that pigs with the E529G mutation had viral loads two orders of magnitude lower than wild-type(WT)pigs,confirming effective resistance to *** of the physiological indicators and scavenger function of CD163 verified no significant differences between the WT and E529G *** findings suggest that E529G pigs can be used for breeding PRRSV-resistant pigs,providing novel insights into controlling future PRRSV outbreaks.

关键词： PRRSV CD163 Point mutation E529G Pigs

The effect of SARS-CoV-2 infection on human embryo early development:a multicenter prospective cohort study

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Science China(Life Sciences) 2023年第7期66卷 1697-1700页

作者： Xiaolei Chen Huangcong Shi Cheng Li Wanxia Zhong Linlin Cui Wenjun Zhang Ling Geng Kuona Hu Mei Fang Daimin Wei Junhao Yan Yun Sun Keliang Wu Han Zhao Zi-Jiang Chen center for reproductive Medicine Shandong UniversityJinan 250012China research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical SciencesJinan 250012China Key Laboratory of reproductive Endocrinology of Ministry of Education Shandong UniversityShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthJinan 250012China national research center of assisted reproductive technology and reproductive genetics Shandong UniversityJinan 250012China center for reproductive Medicine The Second HospitalCheeloo College of MedicineShandong UniversityJinan 250012China center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai Key Laboratory for Assisted Reproduction and Reproductive GeneticsShanghai 200127China

Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCo V-2)infection has swept the globe for 3 years(Zhou et al.,2020).With the nationwide relaxation of controls on the coronavirus disease 2019(COVID-19)epidemic since December 2022 in China,fertility and in vitro fertilization(IVF)centers are receiving increasing numbers of infected patients.

关键词： infection acute

Single-copy Loss of Rho Guanine Nucleotide Exchange Factor 10(arhgef10)Causes Locomotor Abnormalities in Zebrafish Larvae

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Biomedical and Environmental Sciences 2022年第1期35卷 35-44页

作者： ZHANG Yi AN Ming Xing GONG Chen LI Yang Yang WANG Yu Tong LIN Meng LI Rong TIAN Chan Department of Obstetrics and Gynecology Center for Medical GeneticsSchool of Basic Medical SciencesThird HospitalPeking UniversityBeijing 100191China Key Laboratory of assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China Beijing Key Laboratory of reproductive Endocrinology and assisted reproductive technology Beijing 100191China national Clinical research center for Obstetrics and Gynecology Beijing 100191China Department of Medical genetics Center for Medical GeneticsPeking University Health Science CenterBeijing 100191China

Objective To determine if ARHGEF10 has a haploinsufficient effect and provide evidence to evaluate the severity,if any,during prenatal *** Zebrafish was used as a model for generating *** pattern of arhgef10 expression in the early stages of zebrafish development was observed using whole-mount in situ hybridization(WISH).CRISPR/Cas9 was applied to generate a zebrafish model with a single-copy or homozygous arhgef10 *** and light/dark tests were performed in arhgef10^(−/−),arhgef10^(+/−),and wild-type zebrafish ***10 was knocked down using small interferon RNA(siRNA)in the SH-SY5Y cell line,and cell proliferation and apoptosis were determined using the CCK-8 assay and Annexin V/PI staining,*** WISH showed that during zebrafish embryonic development arhgef10 was expressed in the midbrain and hindbrain at 36-72 h post-fertilization(hpf)and in the hemopoietic system at 36-48 *** zebrafish larvae with single-copy and homozygous arhgef10 deletions had lower exercise capacity and poorer responses to environmental changes compared to wild-type zebrafish ***,arhgef10^(−/−) zebrafish had more severe symptoms than arhgef10^(+/-) *** of ARHGEF10 in human neuroblastoma cells led to decreased cell proliferation and increased cell *** Based on our findings,ARHGEF10 appeared to have a haploinsufficiency effect.

关键词： arhgef10 Zebrafish CRISPR/Cas9 Haploinsufficiency Copy loss

Low anti-mullerian hormone decreased clinical pregnancy and increased risk of poor ovarian response in women over 35 years of age

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Chinese Medical Journal 2023年第4期136卷 499-501页

作者： Jiaqi Chang Lihong Xu Yiming Qin Ran Liu Chenxi Li Shanshan Gao Yujie Dang center for reproductive Medicine Key Laboratory of Reproductive Endocrinology of Ministry of EducationShandong Key Laboratory of Reproductive MedicineShandong Provincial Clinical Research Center for Reproductive HealthNational Research Center for Assisted Reproductive Technology and Reproductive GeneticsCheeloo College of MedicineShandong UniversityJinanShandong 250012China Department of Gynaecology and Obstetrics Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandong 250021China Shandong First Medical University JinanShandong 250021China

To the Editor:Infertility affects 15%to 20%of couples worldwide,and assisted reproductive technology(ART)is the most effective treatment *** addition to female age,many other factors,such as antral follicle count(AFC),basal follicle-stimulating hormone(FSH)level,and anti-Mullerian hormone(AMH)level,collectively known as biomarkers for ovarian reserve tests(ORTs),are widely used to predict ovarian responses and assisted-pregnancy outcomes.[1]Among them,AMH and FSH levels were discordant in 20%to 43%of women undergoing ART treatment,and AMH was proven to be superior in predicting live birth rate(LBR).[2]

关键词： pregnancy collective treatment

Impact of vaccination against COVID-19 on the outcomes of in vitro fertilization–embryo transfer:a retrospective cohort study

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Chinese Medical Journal 2023年第2期136卷 207-212页

作者： Jingwen Yin Yang Wang Liyuan Tao Lixue Chen Ping Liu Rong Li center for reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China national Clinical research center for Obstetrics and Gynecology(Peking University Third Hospital) Beijing 100191China research center of Clinical Epidemiology Peking University Third HospitalBeijing 100191China Beijing Key Laboratory of reproductive Endocrinology and assisted reproductive technology Beijing 100191China Key Laboratory of assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China

Background: Vaccination against coronavirus disease 2019 (COVID-19) has become the primary approach in the fight against the spread of COVID-19. Studies have shown that vaccination against COVID-19 has adverse effects, particularly on human reproductive health, despite the fact that vaccination rates are still on the rise. However, few studies have reported whether vaccination affects the outcome of in vitro fertilization-embryo transfer (IVF-ET) or not. In this study, we compared the outcome of IVF-ET and the development of follicles and embryos between vaccinated and unvaccinated groups. Methods: A single-center retrospective cohort study of 10,541 in vitro fertilization (IVF) cycles was conducted from June 2020 to August 2021. 835 IVF cycles with a history of vaccination against COVID-19 and 1670 IVF cycles that served as negative controls were selected and analyzed utilizing the Matchlt package of R software (http://***/) and the nearest neighbor matching algorithm for propensity-matched analysis at a 1:2 ratio. Results: The number of oocytes collected in the vaccinated group and the unvaccinated group were 8.00 (0, 40.00) and 9.00 (0, 77.00) ( P = 0.073) and the good-quality embryo rates of the two groups were 0.56±0.32 and 0.56±0.31 averagely ( P = 0.964). Clinical pregnancy rates for the vaccinated group and unvaccinated group were 42.4% (155/366) and 40.2% (328/816) ( P = 0.486) and biochemical pregnancy rates were 7.1% (26/366) and 8.7% (71/816) ( P = 0.355). Two other factors were analyzed in this study;vaccination among different genders and different types (inactivated vaccine or recombinant adenovirus vaccine) showed no statistically significant effect on the above outcomes. Conclusions: In our findings, vaccination against COVID-19 showed no statistically significant effect on the outcomes of IVF-ET and the development of follicles and embryos, nor did the gender of the vaccinated person or the formulation of vaccines show significant eff

关键词： assisted reproductive treatment COVID-19 Embryo development Pregnancy outcome Vaccination

Expert consensus on the morphological evaluation of human cleavage-stage embryos and blastocysts

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Chinese Medical Journal 2023年第9期136卷 1009-1011页

作者： Da Li Lodovico Parmegiani Dalei Yang Gábor Vajta Rong Li Chinese Association of reproductive Medicine center of reproductive Medicine Shengjing Hospital of China Medical UniversityKey Laboratory of Reproductive and Genetic Medicine(China Medical University)National Health CommissionShenyangLiaoning 110004China Next Fertility GynePro-NextClinics International BolognaItaly RVT Australia CairnsAustralia center for reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalNational Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital)Key Laboratory of Assisted Reproduction(Peking University)Ministry of EducationBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive TechnologyBeijing 100084China 不详

Introduction To date,conventional morphological indicators of embryos are still used as the primary rating parameters or ***,standardized and easy-to-use morphological evaluation criteria for human cleavagestage embryos and blastocysts remain *** to the Istanbul Consensus,[1]morphological scoring systems for cleavage-stage embryos,trophectoderm(TE)cells,and inner cell mass(ICM)include three ratings,namely good,fair,and poor.

关键词： cleavage Expert morphological

Ginsenoside Rb1 inhibits oxidative stress-induced ovarian granulosa cell injury through Akt-FoxO1 interaction

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Science China(Life Sciences) 2022年第11期65卷 2301-2315页

作者： Ping Zhou Feng Deng Zi Yang Canhui Cao Hongcui Zhao Fenting Liu Ke Zhong Lin Fu Tianliu Peng Di Sun Hui Liu Rong Li Yang Yu center for reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China Key Laboratory of assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China Beijing Key Laboratory of reproductive Endocrinology and assisted reproductive technology Beijing 100191China national Clinical research center for Obstetrics and Gynecology(Peking University Third Hospital) Beijing 100191China Clinical Stem Cell research center Peking University Third HospitalBeijing 100191China

Ginsenoside Rb1 shows a strong antioxidant effect and has potential activation effects on *** aim of the present study was to investigate the protective effect of Rb1 on age-related ovarian granulosa cell *** granulosa cells(GCs)were obtained from 50 young women(≤30 years)and 50 aged women(≥38 years)at an IVF *** and aged ICR mice were administered with or without Rb1(10 mg kg^(-1),i.p.)for 2 *** protective effects of Rb1 were investigated and the role of Rb1 on the modulation of Akt-FoxO1 interaction was determined with immunofluorescence,Western blotting,immunoprecipitation,si RNA silencing and pharmacological ***1 effectively decreased LDH and MDA,and reversed the apoptotic-related protein levels in h GL cells from old *** results were found in *** addition,the mitochondrial membrane potential was restored and the overaccumulation of ROS was reversed by ***1 preserved peroxide-impaired Akt activation,to some extent,by increasing phosphorylation at ***1 also facilitated p-Akt binding to FoxO1 and promoted the phosphorylation of *** RNA silencing of Akt,Akt inhibitor LY294002,and FoxO1 inhibitor AS1842856 attenuated the effects of *** Rb1 inhibits age-related GCs oxidative damage by activating Akt phosphorylation at Ser473 and by further interaction with FoxO1.

关键词： ginsenoside Rb1 oxidative stress aging granulosa cell Akt FoxO1

Expert consensus on the vitrification of human oocytes and embryos

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Chinese Medical Journal 2023年第23期136卷 2773-2775页

作者： Da Li Yingzhuo Gao Rong Li Chinese Association of reproductive Medicine center of reproductive Medicine Shengjing Hospital of China Medical UniversityShenyangChinaNHC Key Laboratory of Advanced Reproductive Medicine and Fertility(China Medical University)National Health CommissionShenyangLiaoning 110022China center for reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijingChinaNational Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital)BeijingChinaKey Laboratory of Assisted Reproduction(Peking University)Ministry of EducationBeijingChinaBeijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive TechnologyBeijing 100191China 不详

Introduction The cryopreservation of human oocytes and embryos is a key factor in assisted reproductive technology(ART).Based on the most recent guidelines and consensus[1-8]and in combination with clinical practice,the Chinese Association of reproductive Medicine has called on experts to summarize the technical process and details pertaining to the vitrification of human oocytes and *** intention is to provide a set of comprehensive and systematic guidelines for vitrification and improve the quality of services offered at reproductive centers.

关键词： clinical Medicine details

Self-fertilization in a gonochoristic fish yellow catfish

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Aquaculture and Fisheries 2024年第2期9卷 234-244页

作者： Yue Yu Ziyi Lu Min Chen Ya Liu Yang Ding Chenhao Hu Zhigang Shen College of Fisheries Key Lab of Freshwater Animal BreedingMinistry of Agriculture and Rural AffairsWuhan430070China Key Lab of Agricultural Animal genetics Breeding and Reproduction of Ministry of EducationWuhan430070China Engineering technology research center for Fish Breeding and Culture in Hubei Province Wuhan430070China Engineering research center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt of Ministry of Education Huazhong Agricultural UniversityWuhan430070China Hubei Huangyouyuan Fishery Development Limited Company Wuhan430070China

Sex control techniques are in great demand for aquaculture because of the huge benefits of mono-sex *** a previous study on yellow catfish(Tachysurus fulvidraco),intersex individuals were produced by administrating 17α-methyltestosterone(MT)or aromatase inhibitor letrozole(LZ).The ovarian and testicular portions of intersexual gonads developed well and were completely separated,arousing our interest in producing self-fertilizable fish and clone-like fish *** this study,yellow catfish fry were treated with a combination of MT and LZ at a low dose(5 mg kg^(-1)MT,300 mg kg^(-1)LZ)at a low water temperature(27.0℃,mixed sex-producing temperature,LL group)and a high temperature(33.5◦C,male-producing temperature,HL group),and with a high dose(10 mg kg^(-1) MT,600 mg kg^(-1) LZ)at a low water temperature(LH group),during 12-65 days post-hatching,the critical period of sex *** XX and XY intersex were produced with different percentages at the same time through co-treatment with MT and LZ,with or without the help of male-producing *** highest percentage of XY intersex(53%in XY,36%in XX and XY)and XX intersex(47%in XX,25%in XX and XY)was produced in LH and HL groups,*** of gonadal morphology,histology,and sperm dynamic analysis indicate that some XX and XY intersex individuals were capable of self-fertilization through artificial *** reproductive performance of these intersex individuals was then investigated by injecting spawning agents,dissecting gonads,and artificially fertilizing eggs with their own *** XX,XY,and YY genotypic individuals were produced by the self-fertilization of XY intersex *** present study successfully produced high percentages of XX and XY intersex and conducted self-fertilization by mating the eggs and sperm of a single intersex ***-fertilization in fish will show great potential in aquaculture genetics and breeding,for example,generating high

关键词： Sex differentiation Self-fertilization Sex reversal Mono-sex Clone