Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage

作     者：Kuangdi Luo Yang Qin Xuehao Zhang Hanxu Ji Heao Zhang Jiangtian Li Xianjin Xiao Xinyu Wang Kuangdi Luo;Yang Qin;Xuehao Zhang;Hanxu Ji;Heao Zhang;Jiangtian Li;Xianjin Xiao;Xinyu Wang

作者机构：Department of Breast SurgerySecond Hospital of Jilin UniversityChangchun 130041China Institute of Reproductive HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Laboratory MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pancreatic SurgeryUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China School of Life Science and TechnologyWuhan Polytechnic UniversityWuhan 430023China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第7期

页      面：372-376页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：the financial support from the National Key Research and Development Program of China(No.2021YFC2701402) the Open Research Fund of State Key Laboratory of Bioelectronics,Southeast University(No.Sklb2021-k06) the Open Foundation of NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child Health Care Hospital)(No.KF2020007) Hunan Province Assisted Reproduction and Regenerative Medicine Clinical Demonstration Center Funded Project(No.2020SK4019) the Open Foundation of Translational Medicine National Science and Technology Infrastructure(Shanghai)(No.TMSK-2021-141) 

主　　题：DNA circuit Toehold lock DNA strand displacement Reaction sequence Seesaw circuit 

摘      要：Strand displacement reaction enables the construction of enzyme-free DNA reaction networks,thus has been widely applied to DNA circuit and *** has the characteristics of high efficiency,universality and ***,the existing regulation tools cannot enable effective control of the reaction sequence,which undoubtedly limits the construction of complex nucleic acid ***,we developed a regulation tool,toehold lock,and achieved strict control of reaction sequence without loss of the main reaction signal ***,we applied the tool to scenarios such as seesaw circuits,AND/OR logic gates,and entropy-driven circuits,and respectively demonstrated its significant superiority compared to the original *** believe that the proposed toehold lock has greatly optimized the efficiency of DNA strand displacement-based networks,and we anticipate that the tool will be widely used in multiple fields.