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Exogenous SA Initiated Defense Response and Multi-signaling Pathway in Tetraploid Potato SD20

Horticultural Plant Journal 2020年第2期6卷 99-110页

作者： Jiayi Zheng Yu Yang Xiao Guo Liping Jin Xingyao Xiong Xiaohui Yang Guangcun Li Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesMinistry of Agriculture and Rural AffairsBeijing 100081China national Vegetable Improvement center Shandong Sub-center/Huang-Huai-Hai Region Scientific Observation and Experimental Station of vegetables Ministry of Agriculture and Rural Affairs/Molecular Biology Key Laboratory of Shandong Facility VegetableInstitute of Vegetables and FlowersShandong Academy of Agricultural SciencesJinan 250100China Hu’nan Provincial engineering research center for Potatoes College of Horticulture and LandscapeHunan Agricultural UniversityChangsha 410128China

Salicylic acid(SA) is an important signaling substance that plays an important role in plant growth, development and disease *** order to further understand the role of the SA pathway in potato disease resistance and identify SA signaling key genes, gene expression profiling of the late blight resistance genotype SD20 was performed under exogenous SA application. A total of 28 572 unigenes were assembled,of which 4 564 were differentially expressed. Analysis of differentially expressed genes(DEGs) showed that multiple signaling pathways such as SA, jasmonic acid, ethylene, abscisic acid, auxin, and brassinolide were involved in response to exogenous SA. Many plant defense signalrelated genes involved in protein serine/threonine kinase activity and plant-pathogen interaction, were significantly enriched. These were consistent with the interaction results of SD20 and Phytophthora infestans in our previous study, indicating that exogenous SA stimulated the resistance response and initiated a similar defense pathway compared to pathogen infection in SD20, which confirmed crosstalk of the SA signaling pathway with a pathogen-induced disease resistance signal pathway in plant. Moreover, transcriptome analysis revealed that ROS1 was positively regulated by SA in potato for the first time. SA-induced gene expression profiling provides insight into SA signaling and its mechanisms in disease defense systems.

关键词： potato salicylic acid transcriptome pathway defense response

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Dual-Roof Solar Greenhouse—A Novel Design for Improving the Heat Preserving Capacity in Northern China

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Natural Resources 2014年第12期5卷 681-686页

作者： Lilong Chai Baoju Wang Mingchi Liu Zhanhui Wu Yong Xu national engineering research center for vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing China national engineering research center for vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing China Key Laboratory of Urban Agriculture (North) Ministry of Agriculture Beijing China national engineering research center for vegetables Beijing Academy of Agriculture and Forestry Sciences Beijing China Key Laboratory of Urban Agriculture (North) Ministry of Agriculture Beijing China

Dual-roof solar greenhouse, a new style of solar greenhouse, was designed in this study intending to reduce heat loss in cold time and improve land use efficiency in Beijing, the Capital city of China. Designing and applying the dual-roof greenhouse in metropolitan area had dual effects of saving energy and enhancing land use efficiency. According to the monitoring study and analysis conducted in winter of 2012, the averaged night temperature of south room was about 12.1°C in December, which was satisfying for growing average leaf vegetables. Total energy saved by dual-roof in whole winter was quantified as 1.1 × 107 MJ.yr-1 (winter), potentially about 37.4 t coal was saved in Beijing area during whole winter-growing period. Considering the application of north room, the land use efficiency was improved by 62.5% in dual-roof solar greenhouse.

关键词： Agricultural Resource Dual-Roof Greenhouse Solar Energy Land Use Efficiency Heat Storage

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Nodulin 26-like intrinsic protein Cs NIP2;2 is a silicon influx transporter in Cucumis sativus L.

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Journal of Integrative Agriculture 2022年第3期21卷 685-696页

作者： DUAN Yao-ke SU Yan HAN Rong SUN Hao GONG Hai-jun Shaanxi engineering research center for vegetables/College of Horticulture Northwest A&F UniversityYangling 712100P.R.China Henan Key Laboratory of Ion-Beam Bioengineering School of Agricultural SciencesZhengzhou UniversityZhengzhou 450000P.R.China

Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small *** NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of *** is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely ***,we report the functional characteristics of Cs NIP2;*** was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter *** NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix *** laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low *** NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other *** expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the *** results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other *** findings may be helpful for understanding the functions of NIPs in cucumber plants.

关键词： cucumber(Cucumis sativus L.) nodulin 26-like intrinsic membrane protein(NIP) silicon influx transporter aromatic/arginine selectivity filter

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Impacts of climate change on water quantity, water salinity, food security, and socioeconomy in Egypt

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Water Science and engineering 2021年第1期14卷 17-27页

作者： Mohie El Din Mohamed Omar Ahmed Moustafa Ahmed Moussa Reinhard Hinkelmann national Water research center Fum Ismailiya CanalShoubra El-Kheima 13411Egypt International center for Agricultural research in the Dry Areas Cairo 2416Egypt Nile research Institute(NRI) National Water Research CenterEl-Qanater 13621Egypt Department of Water engineering Technische Universit€at Berlin(TU Berlin)Campus El GounaEl Gouna 84513Egypt

Climate change might have direct impacts on water quantity in Egypt and lead to indirect effects on Mediterranean saltwater intrusion to groundwater,which exposes agriculture to *** study investigated impacts of climate change on agriculture,with particular regard to food security and socioeconomy,and quantified the effectiveness of cropping pattern adaptation measures by integrating three mathematical *** BlueM model was used for hydrological simulations of Nasser Lake under flooding scenarios to predict the water supply from the High Aswan *** water and salinity balance(WB-SAL)model was adopted to estimate the water salinity in the Nile *** simulated results from the BlueM and WB-SAL models were integrated with the agricultural simulation model for Egypt(ASME)to project cropping patterns,food security,and socioeconomy throughout the *** results showed that future climate change will directly affect the total crop area;crop areas for 13 crop types;the self-sufficiency of wheat,rice,cereal,and maize supplies;and socioeconomic *** proposed cropping pattern adaptation measures focus on fixing the crop areas of rice and orchards and providing half of the population with lentils,maize,onion,vegetables,milk,and *** adaptation measures have the potential to promote food security without causing deterioration of the socioeconomic ***,water availability has much more significant effects on food security and socioeconomy than cropping pattern adaptation measures ***,the country should rationalize water use efficiency and increase water supply.©2021 Hohai *** and hosting by Elsevier *** is an open access article under the CC BY-NC-ND license(http://***/licenses/by-nc-nd/4.0/).

关键词： Climate change Water quantity Salinity Cropping pattern adaptation measures Food security Socioeconomy

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6-Benzylaminopurine treatment maintains the quality of Chinese chive(Allium tuberosum Rottler ex Spreng.) by enhancing antioxidant enzyme activity

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Journal of Integrative Agriculture 2017年第9期16卷 1968-1977页

作者： JIA Li-e LIU Sheng DUAN Xiao-ming ZHANG Chao WU Zhan-hui LIU Ming-chi GUO Shao-gui ZUO Jin-hua WANG Li-bin Key Laboratory of Urban Agriculture (North) Ministry of Agriculture/Key Laboratory of Vegetable Postharvest ProcessingMinistry of Agriculture/Key Laboratory of Vegetable Postharvest ProcessingMinistry of Agriculture/Beijing Key Laboratory of Fruits and Vegetable Storage and ProcessingNational Engineering Research Center for VegetablesBeijing Academy of Agriculture and Forestry Sciences College of Food Science and Technology Yangzhou University

Chinese chive usually develops an off-flavor after a short storage time. To explore effective ways to maintain the postharvest quality of Chinese chive, the effect of exogenous application of 6-benzylaminopurine（6-BA） on postharvest quality and antioxidant activity of chive was evaluated, and the mechanism of the physiological responses of chive to 6-BA treatment was explored. Chives were sprayed for 10 min with 100, 300, or 500 mg L–1 6-BA or with alkaline solution as the control, then stored at（2±1）°C with a relative humidity（RH） of 80–85%. We found that 300 mg L–1 6-BA significantly delayed yellowing and chlorophyll degradation, maintained the total phenolic and flavonoid content, and improved the activities of antioxidant enzymes, including superoxide dismutase（SOD）, catalase（CAT） and peroxidase（POD）. In conclusion, we identified exogenous application of 6-BA as an effective method for maintaining postharvest quality of Chinese chive. In addition, our finding that the activities of antioxidant enzymes increase in response to exogenous 6-BA provides new insights into the mechanism of cytokinin-based postharvest fresh-keeping.

关键词： 6-benzylaminopurine(6-BA) Chinese chive postharvest quality antioxidant activity

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Transcriptional search to identify and assess reference genes for expression analysis in Solanum lycopersicum under stress and hormone treatment conditions

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Journal of Integrative Agriculture 2022年第11期21卷 3216-3229页

作者： DUAN Yao-ke HAN Rong SU Yan WANG Ai-ying LI Shuang SUN Hao GONG Hai-jun Shaanxi engineering research center for vegetables/College of Horticulture Northwest A&F UniversityYangling 712100ShaanxiP.R.China Henan Key Laboratory of Ion-Beam Bioengineering College of Agricultural SciencesZhengzhou UniversityZhengzhou 450000HenanP.R.China

Tomato(Solanum lycopersicum)is a model plant for research on fruit development and stress response,in which gene expression analysis is frequently *** PCR(qPCR)is a widely used technique for gene expression analysis,and the selection of reference genes may affect the accuracy of results and even *** there have been some frequently used reference genes in tomato,it has been shown that the expressions of some of these genes are not constant in different tissues and environmental ***,little information on genomic identification of reference genes is available in ***,we mined the publicly available transcriptional sequencing data and screened out fifteen candidate reference genes,and the expression stability of these candidate genes and seven traditionally used ones were evaluated under stress and hormone *** results showed that over half of the selected candidate references were housekeeping genes in tomato *** the candidate reference genes and the traditionally used ones,the most stably expressed genes varied under different treatments,and most of these genes were recommended as preferred reference genes at least once except Solyc04g009030 and Solyc07g066610,two traditionally used reference *** study provides some novel reference genes in tomato,and the preferred reference genes under different environmental stimuli,which may be useful for future *** study suggests that excavating stably expressed genes from transcriptome sequencing data is a reliable approach to screening reference genes for qPCR analysis.

关键词： tomato(Solanum lycopersicum) gene expression qPCR reference gene expression stability

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Flavonoid-producing tomato plants have a direct negative effect on the zoophytophagous biological control agent Oriussauteri

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Insect Science 2023年第1期30卷 173-184页

作者： Fengbo Yang Xinyi Zhang Haowei Shen Hu Xue Tian Tian Qinghe Zhang Jinyu Hu Hong Tong Youjun Zhang Qi Su Hubei engineering Technology center for Forewarning and Management of Agricultural and Forestry Pests College of AgricultureYangtze UniversityJingzhouHubei ProvinceChina Institute of vegetables and Flowers Chinese Academy of Agricultural SciencesBejingChina

Orius sauteri(Poppius)(Hemiptera:Anthocoridae)is often used for biological control of small arthropod pests in greenhouse vegetable production systems in *** addition to feeding on arthropod prey,*** consumes small quantities of plant *** studies demonstrated that tomato plant chemistry confers antixenosis resistance to phloem-feeding whiteflies,but the potential nontarget effects of phytochemicals on the beneficial predator *** are *** of *** confined to near-isogenic lines(NILs)of tomatoes producing high levels offlavonoids(NIL-purple hypocotyl;resistant to whiteflies)and low levels of flavonoids(NIL-green hypocotyl;susceptible to whiteflies)revealed that *** had reduced oviposition,nymphal survival,and development on resistant plants,even if they were also provided with prey that did not feed on the host ***,*** showed a significant ovipositional preference in choice assays,laying significantly more eggs on susceptible than on resistant *** gut content analysis using the specific chloroplast trnL gene from tomato confirmed that adult and immature *** feed on both resistant and susceptible genotypes,and feeding behavior assays revealed that resistance did not affect plant feeding or prey acceptance by *** *** results demonstrate a direct negative effect of phytochemicals on a nontarget beneficial species and indicate that resistance mediated by phytochemicals can affect organisms that do not solely feed on phloem *** results also indicate that the mode of action and the potential ecological effects of phytochemicalmediated resistance are broader than previously recognized.

关键词： biological control host plant resistance insect resistance integrated pest management natural enemy phytochemistry

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Current status of genetic transformation technology developed in cucumber(Cucumis sativus L.)

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Journal of Integrative Agriculture 2015年第3期14卷 469-482页

作者： WANG Shun-li Seong Sub Ku YE Xing-guo HE Cong-fen Suk Yoon Kwon Pil Son Choi Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture/Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Department of Oriental Pharmaceutical Development Nambu University Institute of Crop Science Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement School of Sciences Beijing Technology and Business University Plant Systems engineering research center

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

关键词： Cucumis sativus L. plant regeneration genetic transformation positive selection system

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Infection density pattern of Cardinium affects the responses of bacterial communities in an invasive whitefly under heat conditions

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Insect Science 2023年第4期30卷 1149-1164页

作者： Kun Yang Peng-Hao Qin Meng-Ying Yuan Lei Chen You-Jun Zhang Dong Chu Shandong engineering research center for Environment-Friendly Agricultural Pest Management College of Plant Health and MedicineQingdao Agricultural UniversityQingdaoShandong ProvinceChina Department of Entomology Nanjing Agricultural UniversityNanjingChina Department of Plant Protection Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBejingChina

Communities of bacteria,especially symbionts,are vital for the growth and development of insects and other arthropods,including Bemisia tabaci Mediterranean(MED),a destructive and invasive insect ***,the infection density patterns and influence factors of bacteria in whiteflies,which mainly include symbionts,remain largely *** reveal the different density patterns of Cardinium in *** MED popula-tions and the impacts of high temperatures on whiteflies with different Cardinium density infection patterns,2 isofemale lines isolated from *** MED from the same geo-graphical population of China and from *** MED collected from other countries and locations were examined using several techniques and methods,including fluorescence in situ hybridization(FISH),quantitative real-time polymerase chain reaction(qPCR),16S rRNA gene sequencing,and 2b-RAD *** results showed that there were 2 different infection density patterns of Cardinium in *** MED(including 1 high-density pattern and 1 low-density pattern).For whiteflies with low-density Cardinium,conventional PCR could not detect Cardinium,but the other techniques confirmed that there was a low level of Cardinium within *** temperature significantly decreased the diversity of bacterial communities:the relative titer of Cardinium increased but the density of Rickettsia decreased in the isofemale line with high-density ***-ever,high temperature did not influence the diversity and symbiont density in the line with low-density ***,high temperature influenced the functions of bacterial communities in whiteflies with high-density Cardinium but did not affect the bacterial functions in whiteflies with low-density *** results provide novel insights into the complex associations between symbionts and host insects.

关键词： bacterial communities Bemisia tabaci MED Cardinium heat stress infec-tion density pattern

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MaBEL1 regulates banana fruit ripening by activating cell wall and starch degradation-related genes

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植物学报:英文版 2023年第9期65卷 2036-2055页

作者： Zunyang Song Xiaoyang Zhu Xiuhua Lai Hangcong Chen Lihua Wang Yulin Yao Weixin Chen Xueping Li Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and vegetables/engineering research center for Postharvest Technology of Horticultural Crops in South China Ministry of EducationCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China Key Laboratory of Food Processing Technology and Quality Control in Shandong Province College of Food Science and EngineeringShandong Agricultural UniversityTai'an 271018China

Banana is a typical subtropical fruit, sensitive to chilling injuries and prone to softening ***, the underlying regulatory mechanisms of the softening disorder caused by cold stress remain obscure. Herein, we found that BEL1-LIKE HOMEODOMAIN transcription factor 1(MaBEL1)and its associated proteins regulate the fruit softening and ripening process. The transcript and protein levels of MaBEL1 were up-regulated with fruit ripening but severely repressed by the chilling stress. Moreover, the MaBEL1 protein interacted directly with the promoters of the cell wall and starch degradation-related genes, such as MaAMY3, MaXYL32, and MaEXP-A8. The transient overexpression of MaBEL1 alleviated fruit chilling injury and ripening disorder caused by cold stress and promoted fruit softening and ripening of “Fenjiao” banana by inducing ethylene production and starch and cell wall degradation. The accelerated ripening was also validated by the ectopic overexpression in ***, MaBEL1-silencing aggravated the chilling injury and ripening disorder and repressed fruit softening and ripening by inhibiting ethylene production and starch and cell wall degradation. MaABI5-like and MaEBF1, the two positive regulators of the fruit softening process,interacted with MaBEL1 to enhance the promoter activity of the starch and cell wall degradationrelated genes. Moreover, the F-box protein MaEBF1 does not modulate the degradation of MaBEL1, which regulates the transcription of MaABI5-like protein. Overall, we report a novel MaBEL1-MaEBF1-MaABI5-like complex system that mediates the fruit softening and ripening disorder in “Fenjiao” bananas caused by cold stress.

关键词： cold stress fruit ripening interaction MaBEL1 softening disorder transcriptional regulation

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