Nodulin 26-like intrinsic protein Cs NIP2;2 is a silicon influx transporter in Cucumis sativus L.

作     者：DUAN Yao-ke SU Yan HAN Rong SUN Hao GONG Hai-jun DUAN Yao-ke;SU Yan;HAN Rong;SUN Hao;GONG Hai-jun

作者机构：Shaanxi Engineering Research Center for Vegetables/College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China Henan Key Laboratory of Ion-Beam BioengineeringSchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450000P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第3期

页      面：685-696页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the National Key Research and Development Program of China (2018YFD1000800) the National Natural Science Foundation of China (32072561 and 31772290) 

主　　题：cucumber(Cucumis sativus L.) nodulin 26-like intrinsic membrane protein(NIP) silicon influx transporter aromatic/arginine selectivity filter 

摘      要：Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small *** NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of *** is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely ***,we report the functional characteristics of Cs NIP2;*** was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter *** NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix *** laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low *** NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other *** expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the *** results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other *** findings may be helpful for understanding the functions of NIPs in cucumber plants.