检索结果-南通市图书馆

Identification of serum immunoglobulin M(IgM)of crucian carp(Carassius auratus gibelio)and immune response to cyprinid herpesvirus 2 infection

引用

Aquaculture and fisheries 2024年第2期9卷 172-180页

作者： Shuran Wang Haotian Wu Longlong Wang Pinhua Wang Liqun Lu Yousheng Jiang Ye Zhang Dan Xu national Pathogen Collection center for Aquatic Animals Shanghai Ocean UniversityShanghai201306China Key Laboratory of Aquatic Genetic Resources of the Ministry of Agriculture Shanghai Ocean UniversityShanghai201306China national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai201306China

Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish immunology.Polyclonal antibodies were advantageous molecular tools for studying teleost immune system.Specifically,polyclonal antibodies reacting with immunoglobulins(Ig)were used successfully in studies of the teleost fishes.In the present study,we produced polyclonal antibody against CH2 domains of crucian carp IgM,and measured the in vivo dynamics of IgM mRNA caused by CyHV-2 infection.The recombinant protein IgM with relative molecular weight about 53 KD was correctly expressed in prokaryotic cells.The specificity of the polyclonal antibody was evaluated by Western blotting and results revealed that the antibody not only specifically recognized crucian carp serum but also cross-reacted with grass carp serum.Quantitative RT-PCR analysis demonstrated the expression of IgM mRNA changed significantly after CyHV-2 infection.The expression of IgM in the kidney increased and reached a maximum at 6 h post-infection(hpi),while dropped to a low level at 5 days post-infection(dpi).In conclusion,the expression of IgM was significantly upregulated in the kidney of crucian carp infected with CyHV-2,indicating that IgM played a potential role in systemic immunity against viral infection.Polyclonal antibody against crucian carp IgM had certain clinical relevance,which might provide insight into the early stage of virus infection and prevention of the disease.

关键词： Immunoglobulin M Crucian carp Polyclonal antibody Cyprinid herpesvirus 2 Immunoprotection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Production and characterization of monoclonal antibodies against grass carp CD4-1 and CD4-2

引用

Aquaculture and fisheries 2024年第2期9卷 154-163页

作者： Bangjie Wang Yuting Qin Zhaosheng Sun Xiaozhen Zhu Zhao Jia Kangyong Chen Danjie Liu Junya Wang Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research center for Marine Biosciences at shanghai ocean university Ministry of Science and TechnologyShanghai201306China national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China

CD4 T helper cells are an important group of cells in the immune system of vertebrates and express CD4 receptor on the cell surface.In mammals,the CD4 receptor is encoded by a single copy gene,whilst in fish,two copies of cd4 genes,namely cd4-1 and cd4-2,are found.In this study,the ectodomains of grass carp(Ctenopharyngodon idella,Ci)CD4-1 and CD4-2 were expressed in the E.coli cells and used to generate monoclonal antibodies in mice.Western blotting,confocal microscopy and flow cytometry were performed to characterize the monoclonal antibodies.It has been shown that the CiCD4-1 and CiCD4-2 monoclonal antibodies had good specificity to react with the recombinant ectodomains of CiCD4-1 and CiCD4-2 expressed in the CHO-S cells and the native CD4 molecules of grass carp.The CiCD4-1 monoclonal antibody did not recognize CiCD4-2 and verse versa.In addition,the CD4-1 and CD4-2 monoclonal antibodies specifically recognized the CD4-1 and CD4-2 receptors expressed in the HEK293 cells and native molecules of fish cells.Further,the percentages of lymphocytes in immune tissues of healthy fish were analyzed by flow cytometry.It was found that 17.6%of lymphocytes were CD4-1^(+)cells and 22.5%were CD4-2^(+)in the head kidney.In the spleen,13.1%of lymphocytes were CD4-1^(+)and 18.6%were CD4-2^(+)while 7.3%of blood lymphocytes were CD4-1^(+)cells and 8.8%were CD4-2^(+)cells.The availability of the CD4-1 and CD4-2 monoclonal antibodies provides antibody-based tools for further elucidation of the functions of CD4 T cells in grass carp.

关键词： Grass carp CD4 Monoclonal antibodies Flow cytometry Confocal microscopy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

RNA-seq analysis of a zebrafish caudal fin cell line in response to infection with spring viraemia of carp virus

引用

Aquaculture and fisheries 2024年第2期9卷 181-192页

作者： Wenji Huang Ning Ji Xin Zhao Jiahong Guo Jianhua Feng Kangyong Chen Yaxin Wu Junya Wang Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China

A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid transfection efficiency of 13.9%.Using an RNA-seq approach,differentially expressed genes(DEGs)regulated by SVCV were identified.Infection of SVCV gave rise to 3931 DEGs and up-regulated DEGs were mostly enriched into the biological regulation and cellular processes,among which pathways for the type I interferon signaling and the response to exogenous dsRNA were the top two GO terms.Several KEGG signaling pathways including TLR signaling pathway,RLR receptor signaling pathway,cytosolic DNA-sensing pathway,NLR signaling pathway,cytokine-cytokine receptor interaction and ferroptosis were significantly enriched.Antiviral genes including ifnφ1,isg15 and mx were significantly up-regulated.In addition,key DEGs involved in autophagy were identified.The results indicate that the ZFIN cell line provides a useful in vitro tool for study on the gene functions and cellular responses to viral infection in fish.

关键词： Caudal fin cells SVCV RNA-seq DEGs Innate antiviral response

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterisation of IL-21 and IL-21Rαin grass carp:IL-21-producing cells are upregulated during Flavobacterium columnare infection

引用

Aquaculture and fisheries 2024年第2期9卷 126-135页

作者： Jiawen Xu Junya Wang Qingyu Min Wei Wang Yuting Qin Lina Lei Qian Gao Jun Zou Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research center for Marine Biosciences at shanghai ocean university Ministry of Science and TechnologyShanghai201306China national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266200China

Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune cells.This study characterised a high affinity binding receptor of IL-21(IL-21 Rα)from grass carp(Ctenopharyngodon idella,Ci)and analyzed the expression of CiIL-21 and CiIL-21 Rα.It has been shown that they were modulated by PAMPs and during infection with bacterial and viral pathogens.Recombinant CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses.A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in E.coli cells,and HEK293 T cells and the native protein from PHA-stimulated kidney leukocytes.In addition,the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney,spleen and hindgut after infection with Flavobacterium columnare.Our data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.

关键词： IL-21 IL-21Rα Gene expression IL-21-Producing cell Bacterial and viral infection Fish

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Path analysis of desirable traits and evaluation of reproductive performance of Crassostrea sikamea in different ages

引用

Aquaculture and fisheries 2024年第2期9卷 164-171页

作者： Guangqiu Chang Sheng Liu Hongqiang Xu Minhai Liu Zhihua Lin Qinggang Xue national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai201306China Ninghai Institute of Mariculture Breeding and Seed Industry Zhejiang Wanli UniversityNinghaiZhejiang Province315604China Key Laboratory of Aquatic Germplasm Resources of Zhejiang Zhejiang Wanli UniversityNingbo315100China

Kumamoto oysters(Crassostrea sikamea)are important marine aquaculture shellfish because of their taste and round shell shape.Due to its small size,selective breeding is required to improve its market size,but there are no reports on traits suitable for direction selection and reproductive productivity of Kumamoto oysters of different ages.In this study,path analysis and one-way ANOVA were used to explore the influential indices of oyster body weight and fecundity.The results showed that shell height was the main factor affecting the body weight of Kumamoto oysters at one-year-old and two-year-old,followed by shell length.Body weight was the main factor influencing fecundity,and shell height showed a significant correlation with fecundity.Therefore,shell height should be considered the first parameter for selection when selecting Kumamoto oysters for body weight as the direct selection target.When one-year-old and two-year-old Kumamoto oysters were selected as parents from Xiangshan Bay,hatching parameters and growth parameters of F1 generation larvae were measured.The fecundity and egg diameters were 1.72×10^(6)and 46.60±3.62μm,respectively,which were significantly greater than one-aged oysters(5.86×10^(5),40.26±3.52μm).The hatching parameters(fertilized egg size,fertilization rate,and hatching rate)and larval growth performance(D-stage veliger and veliger)were similar and did not differ significantly(P>0.05),and after metamorphosis,there was also no significant difference in shell height size at 40 days of age(P>0.05).Therefore,there was no significant effect of age on reproduction,larval development,and the early growth of Kumamoto oysters.These results could provide a basis for parental sources and proper traits for Kumamoto oyster selection,which is beneficial to developing and utilizing this excellent germplasm resource.

关键词： Crassostrea sikamea Path analysis Reproductive performance Growth traits

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects ofβ-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated transport

引用

Aquaculture and fisheries 2024年第2期9卷 265-272页

作者： Bo Wu Zhenkun Xu Jie Cao Qi Wang Jun Mei Jing Xie College of Food science and Technology Shanghai Ocean UniversityShanghai201306China national experimental Teaching demonstration center for Food science and Engineering Shanghai Ocean UniversityShanghai201306China shanghai Engineering Research center of Aquatic Product Processing and Preservation Shanghai201306China shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation Shanghai201306China

Transport in water is the most common method for transporting live fish in China,however,transport is a strong stressor.Transport stress could lead to a reduced immune and antioxidant system function of tiger grouper,resulting in sickness and death.Besides,tiger grouper were continuously stressed during transport,which resulted in quality deterioration.It is necessary that find a way to relieve the stress of transportation of tiger grouper.Ascorbic acid is not only a good anti-stress agent,but it is also an effective immunostimulant.β-1,3-glucan is a feed additive that can enhance the immune response of fish.Therefore,this study evaluated the effects ofβ-1,3-glucan and ascorbic acid on the nutritional-immune response and antioxidant signaling pathways of live tiger grouper during simulated transport.Results indicated that addingβ-1,3-glucan and ascorbic acid in transport-water muted the increase of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity.In addition,β-1,3-glucan and ascorbic acid activated Nrf2 and mediated TOR expression and then up-regulate related mRNA expression of antioxidant and immune enzymes.We concluded that the application ofβ-1,3-glucan and ascorbic acid inhibit the increase of metabolism enzymes and inflammatory factors and activate immune and antioxidant signaling pathways to relieve oxidant stress,immune response,and apoptosis.Reducing the loss of amino acids provided nutrients to relieve oxidative stress and immune response,which demonstrated immune-nutritional response in live tiger grouper during simulated transport.These results may provide a new solution for alleviating the decline of immune and antioxidant function of tiger grouper caused by transportation stress.

关键词： Tiger grouper β-1,3-Glucan Ascorbic acid Immune response Antioxidant signaling pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Combined ATAC-seq,RNA-seq,and GWAS analysis reveals glycogen metabolism regulatory network in Jinjiang oyster(Crassostrea ariakensis)

引用

Zoological Research 2024年第1期45卷 201-214页

作者： Biao Wu Xi Chen Jie Hu Zhen-Yuan Wang Yan Wang Da-You Xu Hao-Bing Guo Chang-Wei Shao Li-Qing Zhou Xiu-Jun Sun Tao Yu Xiao-Mei Wang Yan-Xin Zheng Guang-Yi Fan Zhi-Hong Liu State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoShandong 266071China Laboratory for Marine fisheries science and Food Production Processes Laoshan LaboratoryQingdaoShandong 266071China BGI-Qingdao BGI-ShenzhenQingdaoShandong 266426China national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai 201306China Changdao Enhancement and Experiment Station Chinese Academy of Fishery SciencesYantaiShandong 265800China State Key Laboratory of Agricultural Genomics BGI-ShenzhenShenzhenGuangdong 518083China

Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of oysters.The Jinjiang oyster(Crassostrea ariakensis)is an economically and ecologically important species in China.In the present study,RNA sequencing(RNA-seq)and assay for transposase-accessible chromatin using sequencing(ATAC-seq)were performed to investigate gene expression and chromatin accessibility variations in oysters with different glycogen contents.Analysis identified 9483 differentially expressed genes(DEGs)and 7215 genes with significantly differential chromatin accessibility(DCAGs)were obtained,with an overlap of 2600 genes between them.Notably,a significant proportion of these genes were enriched in pathways related to glycogen metabolism,including“Glycogen metabolic process”and“Starch and sucrose metabolism”.In addition,genome-wide association study(GWAS)identified 526 single nucleotide polymorphism(SNP)loci associated with glycogen content.These loci corresponded to 241 genes,63 of which were categorized as both DEGs and DCAGs.This study enriches basic research data and provides insights into the molecular mechanisms underlying the regulation of glycogen metabolism in C.ariakensis.

关键词： Crassostrea ariakensis Glycogen Transcriptome ATAC GWAS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Behavioral and Physiological Responses of Sea Slug (Onchidium reevesii) to Low-Frequency Noise

引用

Journal of ocean university of China 2022年第6期21卷 1607-1614页

作者： TU Zhihan LI Chenqi JIA Jingjing SHEN Heding national demonstration center for experimental fisheries science education Shanghai Ocean UniversityShanghai 201306China International Research center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai 201306China shanghai Collaborative Innovation center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals Shanghai 201306China

A large amount of marine noise pollution from coastal industrial construction and the water transportation industry affects the growth and development of marine life,which is a big issue threatening marine organisms.However,most studies have focused on vertebrates,such as marine mammals and fishes,and little is known about the effects of noise on invertebrates.Therefore,the impacts of low-frequency noise(100,300,and 500 Hz)on behavioral and physiological responsesof the sea slug(Onchidium reevesii)were investigated.Under laboratory conditions,sea slugs were stimulated with low-frequency noise at 100,300,and 500 Hz for 1 h.Then,hemolymph enzyme activities(glucose,albumin,triglycerides,superoxide dismutase,catalase,and malondialdehyde)were measured,and mRNA expression of the hsp70 gene was detected in hemolymph and the nervous system by quantitative realtime polymerase chain reaction(qRT-PCR)analysis,while expression of the hsp70 protein was analyzed by immunohistochemistry.The results demonstrated that sea slugs were away from the stimulus source under the influence of low-frequency noise compared to the control group.Enzyme activities,as well as hsp70 gene mRNA and protein expression levels,were significantly higher in the noiseexposed groups than those in the control group.Overall,these changes indicate that low-frequency noise caused oxidative stress in sea slugs in vivo,and the oxidative damage gradually increased when the noise frequency was increased from 100 to 500 Hz.

关键词： ocean noise Onchidium reevesii low-frequency noise biochemical stress mollusks

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The double mutations of acvr2aa and acvr2ba leads to muscle hypertrophy in zebrafish

引用

Aquaculture and fisheries 2023年第6期8卷 706-712页

作者： Jinyuan Che Cunjie Hu Qi Wang Chunxin Fan Yufeng Si Xiaoling Gong Baolong Bao Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(shanghai ocean university) Ministry of EducationInternational Research Center for Marine Biosciences at Shanghai Ocean UniversityMinistry of Science and TechnologyNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China

Activin A receptor,type II(Acvr2)is a member of the transforming growth factor beta receptor family and can function as a negative regulator of skeletal muscle mass.Acvr2 plays an important role in regulating muscle development that can inhibit skeletal muscle growth in mice.However,there is very little research reported on the function of acvr2 in muscle development of teleost.In this study,we analyzed the effect of acvr2aa and acvr2ba on muscle development in zebrafish.Growth rates of WT and acvr2a^(-/-b-/-)􀀀were measured from juvenile stage to adult stage.In addition,effects of acvr2 on skeletal muscle were tested in histological,protein and molecular levels.As a result,acvr2a^(-/-b-/-)􀀀exhibited a wider body trunk than WT and showed a significant increase in body weight and width from two months old.Histological analysis of skeletal muscle indicated that the size of muscle fiber in acvr2a^(-/-b-/-)􀀀(female:1809±123μm^(2);male:2261±130μm^(2))was larger than that in WT(709.8±49μm^(2);815±53μm^(2)).In addition,western blot of fast MyLc protein showed the protein synthesis of acvr2a^(-/-b-/-)􀀀are increased.Besides,Histological analysis of heart showed the ventricle area is aslo increased in acvr2a^(-/-b-/-)􀀀.Our results demonstrated acvr2 attends the development of muscle fiber and will cause muscle hypertrophy when they were knocked out in zebrafish.In conclusion,acvr2 in zebrafish can control the development of muscle fibers during posthatch growth.

关键词： acvr2 Muscle fiber Hypertrophy Zebrafish Skeletal muscle growth

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

kdm4aa is required for reproduction and development of zebrafish

引用

Aquaculture and fisheries 2023年第6期8卷 713-719页

作者： Lijie Han Juntao Luo Songya Qu Xueling Shi Junfang Zhang Bingshe Han Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai201306China national demonstration center for experimental fisheries science education College of Fisheries and Life SciencesShanghai Ocean UniversityShanghai201306China

Lysine-specific demethylase 4A(KDM4A)catalyzes demethylation of histone lysine residues,which regulates chromatin state and transcription.In drosophila and mice,KDM4A plays an important role in multiple biological processes including development,aging,metabolism,and immunity,however the functions of KDM4A in fish are still unclear.There are two copies of the kdm4a gene in zebrafish,namely kdm4aa and kdm4ab,kdm4aa was edited using CRISPR/Cas9 technology in the present study,then homozygous kdm4aa mutants(kdm4aa^(-/-)􀀀)were obtained,and loss of kdm4aa was confirmed by sequencing and increased H3K9me3.Whole-mount in situ hybridization showed that kdm4aa is widely expressed during the embryonic development of zebrafish.Compared with WT zebrafish,kdm4aa^(-/-)􀀀zebrafish showed no significant difference in gamete formation and fertilization,but the survival rate of kdm4aa^(-/-)􀀀embryos dramatically reduced to 21%at 26 hpf.Further observation showed that about 80%of survived kdm4aa^(-/-)􀀀zebrafish experienced disruption in stripe formation,and 10%of survived kdm4aa^(-/-)􀀀zebrafish underwent vertebral malformation.Alizarin red S staining demonstrated the abnormal spinal development in kdm4aa^(-/-)􀀀zebrafish.These results indicated that kdm4aa is required for normal embryonic development of zebrafish,loss of kdm4aa function leads to decreased survival during the early stages of zebrafish development and morphological variation in adult zebrafish.

关键词： kdm4aa CRISPR/Cas9 Zebrafish Development

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：