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Determining the in vitro Anti-Aging Effect of the Characteristic Components from eucommia ulmoides

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Journal of Renewable Materials 2022年第12期10卷 3131-3145页

作者： Xuesong Wang Zhihong Wang Qiuling Yang Sheng Peng Mijun Peng Guangdong Provincial Key laboratory of Chemical Measurement and Emergency Test technology Guangdong Provincial Engineering Research Center for Ambient Mass SpectrometryInstitute of AnalysisGuangdong Academy of Sciences(China National Analytical CenterGuangzhou)Guangzhou510070China Guangdong Provincial Key laboratory of Silviculture Protection and UtilizationGuangdong Academy of ForestryGuangzhou510520China national&local united engineering laboratory of integrative utilization technology of eucommia ulmoides Jishou University Jishou427000China

To evaluate the potential anti-aging ability of eucommia ulmoides,four characteristic components(chlorogenic acid,geniposidic acid,aucubin,quercetin)were selected to assess their effects on H_(2)O_(2)-induced oxidative damage model of human umbilical vein endothelial cell(HUVEC).Oxidative damage indexes,inflammatory factors,cell cycle,cell apoptosis,cell senescence,and their related proteins were analyzed by methyl thiazolyl tetrazolium(MTT)assay,enzyme-linked immunosorbent assay(ELISA),propidium iodide(PI)staining,annexin V-FITC/PI double staining,SAβ-galactosidase staining,and western blotting(WB).The results showed that H_(2)O_(2)-induced cell growth inhibition rate decreased as supplementation with characteristic components when compared to H_(2)O_(2) group.Meanwhile,the contents of antioxidant indexes(reactive oxygen species,lactate dehydrogenase,molondialdehyde,superoxide dismutase,glutathione),inflammatory factors(nuclear factor kappa-B,intercellular cell adhesion molecule-1,vascular cell adhesion protein 1),and functional factors(NO,Endothelin-1)in characteristic components treated groups improved if comparison with H_(2)O_(2) group,suggesting the characteristic components of E.ulmoides could alleviate H_(2)O_(2)-induced oxidative damage.Moreover,cell cycle,cell apoptosis,cell senescence,and their related proteins under characteristic components treatment exhibited a better effect than under H_(2)O_(2) treatment,implying the characteristic components could participate in anti-aging via multiple pathways.These results manifested that the characteristic components of E.ulmoides posses the capacity of anti-aging,which provided a basis for investigating the anti-aging ability of E.ulmoides itself.

关键词： Anti-aging eucommia ulmoides characteristic components oxidative damage cell senescence

Botanical drugs for bruises used in the Xiangxi region of China, a place rich in martial culture

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Traditional Medicine Research 2023年第11期8卷 45-54页

作者： Jian-Wu He Hong-Tao Gao Xuan Liu Qiang Li Jin Luo Jin Yan Bin-Sheng Luo national and local united engineering laboratory of integrative utilization technology of eucommia ulmoides Jishou UniversityJishou 416000China College of Biology and Environmental Science Jishou UniversityJishou 416000China Lushan Botanical Garden Jiangxi Province and Chinese Academy of SciencesLushan 332900China.

Background:Xiangxi Tujia and Miao Autonomous Prefecture,located in Hunan Province,China,is a culturally rich region with a diverse population.The locals have a deep martial arts history and possess a wealth of traditional knowledge for treating bruises.Methods:In order to investigate this topic,we conducted ethnobotanical studies by visiting local medicinal markets and conducting interviews.Results:We identified 43 botanical drugs used for treating bruises,categorized into four types:sprains,contusions,strains,and bone injuries.The local people typically prefer to mash these botanical drugs freshly or make medicinal wine for external use.Treatment often involves a combination of drugs to promote blood circulation and remove blood stasis,with medications to dispel rheumatism,clear heat,and detoxify,tailored to the humid climate and high precipitation in mountainous areas.Based on the fidelity level values,the local plant resources with high acceptance,utilization,or potential value in the treatment of bruises included Sargentodoxa cuneata,Lycopodium japonicum,Dioscorea cirrhosa,Panax japonicus,Achyranthes bidentata,Lysimachia heterogenea and so on.Conclusion:Herbal medicine and related knowledge for treating bruises are diverse and rich in the Xiangxi region.However,the local traditional medicinal knowledge faces challenges regarding inheritance due to regional modernization.Therefore,it is crucial to protect and further study this knowledge in the future.

关键词： bruises medicinal plants ethnobotany Xiangxi traditional knowledge

Recovery of natural active molecules using aqueous two-phase systems comprising of ionic liquids/deep eutectic solvents

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Green Chemical engineering 2022年第1期3卷 5-14页

作者： Yicong Wang Shanshan Wang Leilei Liu Key laboratory of Hunan Forest Products and Chemical Industry engineering Jishou UniversityZhangjiajie427000China

Due to environmental protection requirements,extraction of bioactive compounds from plant materials using environment-friendly green solvents has always been a research hotspot.And great efforts of scholars have been made in this direction,as well as environment-friendly solvents have been used to develop many innovative extraction techniques.Ionic liquids(ILs)and deep eutectic solvents(DESs)are two kinds of typical designable green solvents,which are potential replacements for traditional volatile organic solvents used for extracting.Under the substances action of inorganic salts or polymers,ILs/DESs can form an aqueous two-phase system(ATPS),which has obvious advantages for separating natural products.This paper discussed the phase separation principle of ILs/DESs-based ATPSs and reviewed the applications in the extraction of natural active molecules in recent years,as well as to promote the development of separation of the active constituents in Chinese materia medica.

关键词： Ionic liquids Deep eutectic solvents Aqueous two-phase system Natural active molecules Extraction and separation

Dietary protocatechuic acid ameliorates inflammation and up-regulates intestinal tight junction proteins by modulating gut microbiota in LPS-challenged piglets

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Journal of Animal Science and Biotechnology 2021年第1期12卷 328-339页

作者： Ruizhi Hu Ziyu He Ming Liu Jijun Tan Hongfu Zhang De-Xing Hou Jianhua He Shusong Wu Hunan Collaborative Innovation Center for utilization of Botanical Functional Ingredients College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Department of Food Science and Biotechnology Faculty of AgricultureKagoshima UniversityKagoshima 890-0065Japan Beijing China-Agri HongKe Bio-technology Co. Ltd.Beijing 102206China State Key laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China.

Background: Weaning is one of the major factors that cause stress and intestinal disease in piglets. Protocatechuic acid(PCA) is an active plant phenolic acid which exists in Chinese herb, Duzhong(eucommia ulmoides Oliver), and is also considered as the main bioactive metabolite of polyphenol against oxidative stress and inflammation. This study aimed to investigate the effect of PCA on growth performance, intestinal barrier function, and gut microbiota in a weaned piglet model challenged with lipopolysaccharide(LPS).Methods: Thirty-six piglets(Pig Improvement Company line 337 × C48, 28 d of age, 8.87 kg ± 0.11 kg BW) were randomly allocated into 3 treatments and fed with a basal diet(CTL), a diet added 50 mg/kg of aureomycin(AUR), or a diet supplemented with 4000 mg/kg of PCA, respectively. The piglets were challenged with LPS(10 μg/kg BW) on d 14 and d 21 by intraperitoneal injection during the 21-d experiment. Animals(n = 6 from each group) were sacrificed after being anesthetized by sodium pentobarbital at 2 h after the last injection of LPS. The serum was collected for antioxidant indices and inflammatory cytokines analysis, the ileum was harvested for detecting mRNA and protein levels of tight junction proteins by PCR and immunohistochemical staining, and the cecum chyme was collected for intestinal flora analysis using 16 S rRNA gene sequencing.Results: Dietary supplementation of PCA or AUR significantly increased the expression of tight junction proteins including ZO-1 and claudin-1 in intestinal mucosa, and decreased the serum levels of thiobarbituric acid reactive substances(TBARS) and IL-6, as compared with CTL group. In addition, PCA also decreased the serum levels of IL-2 and TNF-α(P < 0.05). Analysis of gut microbiota indicated that PCA increased the Firmicutes/Bacteroidetes ratio(P < 0.05). Spearman's correlation analysis at the genus level revealed that PCA reduced the relative abundance of Prevotella 9, Prevotella 2, Holdemanella, and Ruminococcus torques group(P < 0.05), and increased the relati

关键词： Gut microbiota Inflammation Piglets Protocatechuic acid Tight junction proteins

Overexpression ofβ-1,4-Glucanase Gene EuEG1 Improves Micrografting of eucommia ulmoides

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Phyton-International Journal of Experimental Botany 2023年第11期92卷 3063-3075页

作者： Lei Wang Runying Wang Yi Li Yichen Zhao Degang Zhao The Key laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Tea SciencesCollege of Life SciencesGuizhou UniversityGuiyang550025China Guizhou Plant Conservation technology Center Guizhou Academy of Agricultural SciencesGuiyang550006China Department of Plant Science and Landscape Architecture University of ConnecticutStorrsConnecticutUSA

Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of eucommia ulmoides.Micrografting offers a new method for the transplantation of genetic transformation,and its success depends on the formation of graft unions.This study used transgenic rootless test-tube seedlings as scions and seedlings from seed as rootstocks during micrografting to avoid the rooting issues that occur during tissue culture and to investigate the role of the EuEG1 gene in the graft healing process.We found that the EuEG1 gene is a vital regulator of graft,and its overexpression contributes to the survival of eucommia ulmoides micrografting.The EuEG1 gene transgenic plants(TP)used as scions for micrografting presented a significantly higher survival rate than the wild type(WT)and empty vector(EV)regenerated scions.During the grafting healing process,the expression of the EuEG1 gene was higher during the period of callus proliferation,suggesting that the EuEG1 gene was involved in the graft healing process.Histological observation revealed that more calluses tissue appeared at the junction of transgenic scions,and the connection with the rootstock was stronger,which benefits wound healing.These results provide new insights into eucommia ulmoides micrografting and indicate that the EuEG1 gene can promote wound healing and improve the micrografting survival rate.

关键词： eucommia ulmoides genetic transformation EuEG1 gene micrografting

Cloning and expression analysis of the nuclear factor erythroid 2-related factor 2(Nrf2)gene of grass carp(Ctenopharyngodon idellus)and the dietary effect of eucommia ulmoides on gene expression

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Aquaculture and Fisheries 2018年第5期3卷 196-203页

作者： Junpeng Wang Jun Xie Zhihan Tu Jing Wei Wenqian Pan Jing Hu Yi Zhang Xiangjun Leng Xiaoqin Li Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture Shanghai Ocean UniversityShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai201306China national Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China Chinese Academy of Fishery Sciences Key Laboratory of Freshwater Fisheries and Germplasm Resources UtilizationWuxi214081China Department of Animal Science Xichang UniversityXichang615013China

Nuclear factor erythroid 2-related factor 2(Nrf2)is an important transcription factor in the regulation of redox homeostasis,which plays a vital role in metabolism,the cell cycle,and cell protection.In this study,the complete cDNA sequence of Nrf2 gene of grass carp was obtained by rapid amplification of cDNA ends-polymerase chain reaction(RACE-PCR),and the characteristics of Nrf2 proteins predicted from gene sequencing were analyzed by bioinformatics methods.Tissue differential expression patterns were also detected by Real-time Fluorescence quantitative PCR(qRT-PCR).Then,eucommia ulmoides(EU),a traditional Chinese medicine,was added into diet with an inclusion of 2%to feed grass carp for 3 months to investigate the effects on the expression of Nrf2 gene in tissues.The results showed that the full-length of Nrf2 gene of grass carp is 2760 bp,encoding 593 amino acids,and the predicted Nrf2 protein is a hydrophilic and non-secretory protein.Grass carp Nrf2 gene showed higher expression in kidney,gill,liver,spleen,and blood tissues than in other tissues.In the feeding trial,dietary EU significantly promoted the Nrf2 mRNA expression in kidney,gill,and liver tissues after three months feeding.The results suggest that Nrf2 plays an important role in the anti-oxidative capacity of grass carp and its expression is enhanced by dietary EU.

关键词： Grass carp Nrf2 RACE Bioinformatics analysis eucommia ulmoides