Overexpression ofβ-1,4-Glucanase Gene EuEG1 Improves Micrografting of Eucommia ulmoides

作     者：Lei Wang Runying Wang Yi Li Yichen Zhao Degang Zhao 

作者机构：The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education)College of Tea SciencesCollege of Life SciencesGuizhou UniversityGuiyang550025China Guizhou Plant Conservation Technology CenterGuizhou Academy of Agricultural SciencesGuiyang550006China Department of Plant Science and Landscape ArchitectureUniversity of ConnecticutStorrsConnecticutUSA 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2023年第92卷第11期

页      面：3063-3075页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：y National Natural Science Foundation of China,Grant Number 31870285 Talent Special Project of Guizhou Academy of Agricultural Sciences,Grant Number 2022-02 Talent Base for Germplasm Resources Utilization and Innovation of Characteristic Plant in Guizhou,Grant Number RCJD2018–14 

主　　题：Eucommia ulmoides genetic transformation EuEG1 gene micrografting 

摘      要：Adventitious root formation poses a major constraint on the tissue culture and genetic transformation of Eucommia *** offers a new method for the transplantation of genetic transformation,and its success depends on the formation of graft *** study used transgenic rootless test-tube seedlings as scions and seedlings from seed as rootstocks during micrografting to avoid the rooting issues that occur during tissue culture and to investigate the role of the EuEG1 gene in the graft healing *** found that the EuEG1 gene is a vital regulator of graft,and its overexpression contributes to the survival of Eucommia ulmoides *** EuEG1 gene transgenic plants(TP)used as scions for micrografting presented a significantly higher survival rate than the wild type(WT)and empty vector(EV)regenerated *** the grafting healing process,the expression of the EuEG1 gene was higher during the period of callus proliferation,suggesting that the EuEG1 gene was involved in the graft healing *** observation revealed that more calluses tissue appeared at the junction of transgenic scions,and the connection with the rootstock was stronger,which benefits wound *** results provide new insights into Eucommia ulmoides micrografting and indicate that the EuEG1 gene can promote wound healing and improve the micrografting survival rate.