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A mini-review on pharmacological effects of ginsenoside Rb3,a marked saponin from Panax genus

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BIOCELL 2022年第6期46卷 1417-1423页

作者： WEI LI YUEYANG DUAN XIAOTONG YAN XIANGXIANG LIU MEILING FAN ZI WANG College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun130118China national&local joint engineering research center for ginseng breeding and development Changchun130118China Maternity Diagnosis&Treatment center The Affiliated Hospital of Changchun University of Chinese MedicineChangchun130021China

Ginsenoside Rb3(G-Rb3)is one of the primary active compounds isolated from Panax ginseng Meyer,which belongs to protopanaxadiol ginsenosides(PPD).Based on the structure-activity relationship(SAR)of ginsenosides,the pentose structure of G-Rb3 limited itself to possess more pharmacological activity to a certain ***,pharmacokinetics show that G-Rb3 is processed through deglycosylation in the intestinal tract and converted into more active rare saponins,such as Compound K,F2,etc.A series of studies focused on neuroprotection and the cardiovascular system demonstrating its therapeutic potentials,which was achieved by diminishing oxidative stress and ***,more systematic and in-depth studies are needed to complete the pharmaceutical value and to promote its clinical *** article highlights the multiple pharmacological effects and mechanisms of G-Rb3 and prospects for its development.

关键词： Ginsenoside Rb3 Pharmacological effects Review Signaling pathway

20(R)-ginsenoside Rg3,a product of high-efficiency thermal deglycosylation of ginsenoside Rd,exerts protective effects against scrotal heat-induced spermatogenic damage in mice

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BIOCELL 2020年第4期44卷 655-669页

作者： WEI LIU ZI WANG JING LENG HENG WEI SHEN REN XIAOJIE GONG CHEN CHEN YINGPING WANG RUI ZHANG WEI LI College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun130118China national&local joint engineering research center for ginseng breeding and development Changchun130118China Key Laboratory of Biotechnology and Bioresources Utilization College of Life ScienceDalian Minzu UniversityDalian116600China School of Biomedical Sciences University of QueenslandBrisbane4072Australia

Heat stress(HS)reaction can lead to serious physiological dysfunction associated with cardiovascular and various organ *** Rg3(G-Rg3)is a representative component of ginseng rare saponin and can protect against multiple organs,also used as functional food to adjust the balance of the human body,but the therapeutic effect and molecular mechanism of G-Rg3 on male diseases under HS are *** aim of the present study,G-Rg3 was prepared through the efficient conversion of ginsenoside Rd and investigate the contribution of G-Rg3 to testicular injury induced exposure to *** mice were divided into four groups as follows:normal group,HS group,and HS+G-Rg3(5 and 10 mg/kg)groups.G-Rg3 was administered orally for 14 days,then exposed to a single scrotal heat treatment(43°C,18min)on the 7th *** HS treatment,the morphology of testis and epididymis changes,and caused a significant loss of multinucleated giant cells,desquamation of germ cells in destructive seminiferous tubules,and degenerative Leydig cells,further destroying the production of *** administration G-Rg3(5 and 10 mg/kg/day)for 2 weeks,the spermatogenic-related indexes of testosterone levels and superoxide dismutase(SOD)activity,glutathione(GSH)content significantly(p<0.01)increase compared with the HS ***,G-Rg3 treatment effectively ameliorated the production of malondialdehyde(MDA)(p<0.05 or p<0.01).Importantly,G-Rg3 exhibited the protective potential against HS-induced injury not only suppressing the protein levels of heme oxygenase-1(HO-1),hypoxia-inducible factor-1α(HIF-1α),and heat shock protein 70(HSP70)but also modulating the Bcl-2 family(p<0.01 or p<0.001)and activation of mitogen-activated protein kinase(MAPK)signaling pathways(p<0.01).For most of the parameters tested,the HS+G-Rg3(10 mg/kg)group exhibited potent effects compared with those exhibited by the low dose(5 mg/kg)*** conclusion,the present study demonstrated that G-Rg3 exerted protective

关键词： 20(R)-ginsenoside Rg3 Heat stress Spermatogenic damage Oxidative stress MAPK

Integrative SMRT sequencing and ginsenoside profiling analysis provide insights into the biosynthesis of ginsenoside in Panax quinquefolium

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Chinese Journal of Natural Medicines 2022年第8期20卷 614-626页

作者： DI Peng YAN Yan WANG Ping YAN Min WANG Ying-Ping HUANG Lu-Qi State Key Laboratory breeding Base of Dao-Di Herbs National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China center for Post-doctoral research China Academy of Chinese Medical SciencesBeijing 100700China State local joint engineering research center of ginseng breeding and Application Jilin Agricultural UniversityChangchun 130118China

Panax quinquefolium is one of the most common medicinal plants *** are the major pharmaceutical components in *** biosynthesis of ginsenosides in different tissues of *** remained largely *** the current study,an integrative method of transcriptome and metabolome analysis was used to elucidate the ginsenosides biosynthesis pathways in different tissues of ***,22 ginsenosides in roots,leaves,and flower buds showed uneven distribution patterns.A comprehensive *** transcriptome was generated through single molecular real-time(SMRT)and second-generation sequencing(NGS)technologies,which revealed the ginsenoside pathway genes and UDP-glycosyltransferases(UGT)family genes explicitly expressed in roots,leaves,and flower *** weighted gene co-expression network analysis(WGCNA)of ginsenoside biosynthesis genes,UGT genes and ginsenoside contents indicated that three UGT genes were positively correlated to pseudoginsenoside F11,notoginsenoside R1,notoginsenoside R2 and pseudoginsenoside *** results provide insights into ginsenoside biosynthesis in different tissues ***.

关键词： Panax quinquefolium SMRT sequencing Ginsenoside biosynthesis UDP-glycosyltransferases

Antidepressant-like Effects of Flavonoids from Pteridium aquilinum ***

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Medicinal Plant 2023年第1期14卷 55-63页

作者： Zimeng FENG Xiang HAN Shuangli LIU Xiaohui WANG research center of Agricultural Environment and Resources Jilin Academy of Agricultural SciencesChangchun 130033China national&local joint engineering research center for ginseng breeding and Application Jilin Agricultural UniversityChangchun 130033China

[Objectives]To explore the antidepressant-like effects of PAFL and its potential mechanisms.[Methods]This study used PAFL in mice under various conditions and analyzed the *** antidepressant-like effects of flavonoids from Pteridium aquilinum(PAFL)and its possible action mechanism were discussed.[Results]PAFL(5 mg/kg,i.p.)exhibited markedly antidepressant-like activities,which could be reversed by treatment with p-chlorophenylalanine(an inhibitor of 5-HT synthesis),haloperidol(a non-selective D 2 receptor antagonist)and N-methyl-D-aspartic acid(an agonist at the glutamate site).Meanwhile,PAFL also effectively increased the hippocampus 5-HT,DA,and Glu levels of mice exposed to TST and *** LPS-induced antidepressant behavioral experiment showed that PAFL(1 and 5 mg/kg,i.p.)and fluoxetine(20 mg/kg,i.p.)effectively reduced the immobility time,and increase activity time in *** pretreatment significantly reduced IL-6 and TNF-αserum levels and improve the changes of hippocampal SOD and MDA oxidative stress ***,PAFL preconditioning could up-regulate BDNF and TrκB contents in hippocampus and down-regulate IκB-αand NF-κB phosphorylation.[Conclusions]The antidepressant-like effects of PAFL might be mediated by the 5-HT,DAergic and Gluergic systems,and the mechanisms of anti-depressant effects of PAFL might be via the alterations of animal behaviors,hippocampus inflammation and neurotrophy,which might be attributed by the BDNF/TrκB/NF-κB signaling pathway.

关键词： Pteridium aquilinum var.latiusculum Central nervous system LPS-induced depression Inflammation Neurotrophy

m^(6)A demethylase FTO regulate CTNNB1 to promote adipogenesis of chicken preadipocyte

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Journal of Animal Science and Biotechnology 2023年第2期14卷 578-592页

作者： Kan Li Weichen Huang Zhijun Wang Qinghua Nie Department of Animal Genetics Breeding and ReproductionCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China national‑local joint engineering research center for Livestock breeding Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breedingand Key Laboratory of Chicken GeneticsBreeding and ReproductionMinistry of AgricultureGuangzhou 510642China

Background:N6-methyladenosine(m^(6)A)is an abundant post-transcriptional RNA modification that affects various biological *** fat mass and obesity-associated(FTO)protein,a demethylase encoded by the FTO gene,has been found to regulate adipocyte development in an m^(6)A-dependent manner in multiple ***,the effects of the m^(6)A methylation and FTO demethylation functions on chicken adipogenesis remain *** study aims to explore the association between m^(6)A modification and chicken adipogenesis and the underlying mechanism by which FTO affects chicken preadipocyte ***:The association between m^(6)A modification and chicken lipogenesis was assessed by treating chicken pread-ipocytes with different doses of methyl donor betaine and methylation inhibitor *** results showed that betaine significantly increased methylation levels and inhibited lipogenesis,and the inverse effect was found in preadipocytes after cycloleucine *** of FTO significantly inhibited m^(6)A levels and promoted proliferation and differentiation of chicken *** FTO showed opposite ***,FTO overexpression increased the expression of catenin beta 1(CTNNB1)by improving RNA stability in an m^(6)A-dependent manner,and we proved that FTO could directly target ***,CTNNB1 may be a positive regulator of adipogenesis in chicken ***:m^(6)A methylation of RNA was negatively associated with adipogenesis of chicken *** could regulate CTNNB1 expression in a demethylation manner to promote lipogenesis.

关键词： Adipogenesis Chicken CTNNB1 FTO m^(6)A

Comparative analysis of physicochemical properties,ginsenosides content andα-amylase inhibitory effects in white ginseng and red ginsen

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Food Science and Human Wellness 2023年第1期12卷 14-27页

作者： Huairui Wang Yao Cheng Xue Zhang Yingping Wang Hui Zhao State local joint engineering research center of ginseng breeding and Application College of Chinese Medicinal MaterialsJilin Agricultural UniversityChangchun 130033China School of Biotechnology and Food Science Tianjin University of CommerceTianjin 300134China School of Pharmaceutical Science and Technology Health Sciences PlatformTianjin UniversityTianjin 300072China

ginseng(Panax ginseng ***)as a common dietary adjunct is widely applied in Traditional Chinese Medicine due to its health-promoting properties,but the differences between white ginseng and red ginseng was rarely *** the present study,color parameters and scanning electron microscope(SEM)were determined to evaluate the differences of ginseng color and microstructure induced by processing *** analysis of multi-components by a single-marker(QAMS)method and anti-α-amylase activity test were used to assess variations of chemical ingredients and pharmacological activity between white and red ***,molecular docking studies were carried out to screen out the most effective compound againstα-*** indicated that processing had a significant impact on the physicochemical properties and pharmacological activity of white and red *** processing,the color value of L*declined *** ginseng sample displayed a compact structure and presented of a gel layer on the surface compared to white ***,the content of ginsenosides and the activity of anti-α-amylase *** contents of total ginsenosides were positively correlated with the anti-α-amylase activities of ginseng,and ginsenoside Rb1 might be the most effective compound to inhibit the activity ofα-amylase.

关键词： ginseng Color Microstructure Ginsenosides α-Amylase

Functional characterization of the promoter of carbonyl reductase 1 gene in porcine endometrial cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第7期18卷 626-634页

作者： Ai-ling ZHANG Xian-yue SUN Qi YIN Jian-hua ZENG Zhe ZHANG Jia-qi LI Hao ZHANG Guangdong development center of Applied Ecology and Ecological engineering in Universities Biology and Food Engineering Institute Guangdong University of Education Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular breeding National Engineering Research Center for Breeding Swine Industry National & Local Joint Engineering Research Center for Livestock and Poultry Breeding College of Animal Science South China Agricultural University

Prostaglandins （PGs） play a critical role in porcine reproduction, of which prostaglandin E2 （PGE2） and prostaglandin F2a （PGF2a） exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enzyme, carbonyl reductase 1 （CBR1） catalyzes the conversion of PGE2 to PGF2a. A high ratio of PGE2：PGF2a is beneficial to the establishment and maintenance of porcine pregnancy. PG is essential for the establishment of pregnancy which resembles the proinflammatory response and nuclear factor KB （NF-KB） is involved in the process. Bioinformatic analysis has shown that NF-KB is a possible factor bound to two cis-regulatory elements in CBRI promoter. In this study, we cloned the 2997 bp （-2875/＋122） of the promoter, and constructed six 5＇-deleted dual-luciferase reporter recombinant vectors. In endometrial cells, the region of P2 （-16401＋7） exhibited the greatest transcriptional activity at driving luciferase expression, but not significantly different from that of P1 （-2089/＋7）. The activity of P1, P2, and P3 （-1019/＋7） was highly significantly higher than that of others （P〈0.01）, suggesting that two positive regulatory elements were likely present in the regions of -1640/-1019 and -1019/-647. The results also showed that the -1640/ -647 region was indispensable for the promoter. The results of chromatin immunoprecipitation （CHIP） demonstrated that the NF-KB subunit p65 binds to one site around -15451-1531. Using four reference genes, we found that the over-expression of p65 enhanced the expression of CBR1 （P〈0.05） in porcine endometrial epithelial cells, while knockdown of the p65 did not down-regulate the CBRI expression. These results indicated that NF-KB （p65） could bind to the special element of CBR1 gene promoter in porcine endometrial epithelial cells in vitro. The binding site of NF-KB was a positive regulator for the CBR1 gene promoter, but was not necessary for the basic expression.

关键词： Pig Carbonyl reductase 1 (CBR1) Promoter NF-KB Endometrium

Significant association of the novel Rf4-targeted SNP marker with the restorer for WA-CMS in different rice backgrounds and its utilization in molecular screening

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Journal of Integrative Agriculture 2017年第10期16卷 2128-2135页

作者： CHEN Li-kai YAN Xian-cheng DAI Jun-hao CHEN Si-ping LIU Yong-zhu WANG Hui CHEN Zhi-qiang GUO Tao national engineering research center of Plant Space breeding South China Agricultural University Guangzhou 510642 P.R. China

In the rice cytoplasmic-genetic male sterility （CMS） system, the combination of a CMS line, maintainer line and restorer line carrying the restorer gene to restore fertility, is indispensable for the development of hybrids. However, the process of screening for the trait of fertility restoration is laborious and time-consuming. In the present study, we analyzed the nucleotide sequence of the Rf4 gene, which is the major locus controlling fertility restoration, to identify allele-specific variation. A single nucleotide polymorphism （SNP） A/C at ＋474 in the coding sequence （CDS） was found to be capable of strictly distinguishing groups of alleles Rf4 （A） and rf4 （C）. Using KASP genotyping, this valuable SNP was converted to an allele-specific PCR marker. We evaluated and validated the marker among three-line parents with different backgrounds, and the results revealed a complete correlation between SNP alleles and the fertility restoration phenotype. Molecular screening was subsequently carried out for the presence of alleles of Rf4 and Rf3 among 328 diverse rice cultivars with worldwide distribution. The results demonstrate that this SNP marker could be the optimal choice for the molecular identification of potential restorers.

关键词： hybrid rice Rf4 SNP marker fertility restorer

RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts

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Journal of Integrative Agriculture 2015年第5期14卷 926-938页

作者： XU Wei-hua LI Zi-cong OUYANG Zhi-ping YU Bo SHI Jun-song LIU De-wu WU Zhen-fang national engineering research center for breeding Swine Industry South China Agricultural University College of Life Science Longyan University Wen's research Institute

Somatic nuclear transfer technology has become increasingly promising in biomedicine and *** the approach remains inefficient and underlying mechanisms remain *** cloned embryos have similar in vitro developmental capacity as in vitro fertilized（IVF） embryos before implantation,they appeared to have much lower full-term developmental efficiency in pig and cattle,and thus it would be reasonable to postulate that profound distinction at the molecular level should exist between ***,RNA sequencing technique was used to screen differentially expressed genes in cloned and IVF blastocysts,and in total 628 differentially expressed transcripts were obtained,among which,280 transcripts are up-regulated and 348 transcripts are down-regulated in cloned ***,one statistically significant pathway associated with endoplasmic reticulum（ER） protein processing was enriched,and some ER-stress markers such as ATF4,ATF6,PDIA3,HSPA1 B,HSP40 and HSP90 between cloned and IVF blastocysts were ***,some developmentally important genes such as lipid metabolism related genes（MGLL,DDHD2 and FADS2） and epigenetic modification genes（DNMT1,KDM5 C and MBD3L5） were found differentially expressed between cloned and IVF embryos.

关键词： cloned transcripts RNA porcine developmental endoplasmic reticulum differentially cloning enriched