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Optimizing bone marrow harvesting sites for enhanced mesenchymal stem cell yield and efficacy in knee osteoarthritis treatment

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World Journal of Methodology 2025年第2期15卷 92-107页

作者： Arulkumar Nallakumarasamy Sandeep Shrivastava Ravi Velamoor Rangarajan Naveen Jeyaraman Avinash Gandi Devadas Swaminathan Ramasubramanian Madhan Jeyaraman Department of Orthopaedics Datta Meghe Institute of Higher Education and ResearchWardha 442004MaharashtraIndia Department of regenerative medicine Mother Cell Regenerative CentreTiruchirappalli 620017Tamil NaduIndia Department of Orthopaedics Government Medical CollegeOmandurar Government EstateChennai 600002Tamil NaduIndia Department of Orthopaedics ACS Medical College and HospitalDr MGR Educational and Research InstituteChennai 600077Tamil NaduIndia

Knee osteoarthritis(OA)is a debilitating condition with limited long-term treatment *** therapeutic potential of mesenchymal stem cells(MSCs),particularly those derived from bone marrow aspirate concentrate,has garnered attention for cartilage repair in *** the iliac crest is the traditional site for bone marrow harvesting(BMH),associated morbidity has prompted the exploration of alternative sites such as the proximal tibia,distal femur,and proximal *** paper reviews the impact of different harvesting sites on mesenchymal stem cell(MSC)yield,viability,and regenerative potential,emphasizing their relevance in knee OA *** iliac crest consistently offers the highest MSC yield,but alternative sites within the surgical field of knee procedures offer comparable MSC characteristics with reduced *** integration of harvesting techniques into existing knee surgeries,such as total knee arthroplasty,provides a less invasive approach while maintaining thera-peutic ***,variability in MSC yield from these alternative sites underscores the need for further research to standardize techniques and optimize clinical *** directions include large-scale comparative studies,advanced characterization of MSCs,and the development of personalized harvesting ***,the findings suggest that optimizing the site of BMH can significantly influence the quality of MSC-based therapies for knee OA,enhancing their clinical utility and patient outcomes.

关键词： Knee Osteoarthritis Mesenchymal stem cells Bone marrow harvest regenerative medicine

Advances in regenerative therapies for spinal cord injury:a biomaterials approach

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Neural Regeneration Research 2015年第5期10卷 726-742页

作者： Magdalini Tsintou Kyriakos Dalamagkas Alexander Marcus Seifalian UCL centre for nanotechnology & regenerative medicine Division of Surgery and Interventional ScienceUniversity College of London Royal Free London NHS Foundation Trust Hospital

Spinal cord injury results in the permanent loss of function, causing enormous personal, social and economic problems. Even though neural regeneration has been proven to be a natural mech- anism, central nervous system repair mechanisms are ineffective due to the imbalance of the inhibitory and excitatory factors implicated in neuroregeneration. Therefore, there is growing re- search interest on discovering a novel therapeutic strategy for effective spinal cord injury repair. To this direction, cell-based delivery strategies, biomolecule delivery strategies as well as scaf- fold-based therapeutic strategies have been developed with a tendency to seek for the answer to a combinatorial approach of all the above. Here we review the recent advances on regenerativel neural engineering therapies for spinal cord injury, aiming at providing an insight to the most promising repair strategies, in order to facilitate future research conduction.

关键词： tissue engineering neuroregeneration repair central nervous system biomaterial regenerative medicine nanotechnology spinal cord injury

nanotechnology and bio-functionalisation for peripheral nerve regeneration

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Neural Regeneration Research 2015年第8期10卷 1191-1194页

作者： Tina Sedaghati Alexander M.Seifalian centre for nanotechnology and regenerative medicine Division of Surgery & Interventional Science University College London Royal Free NHS Trust Foundation Hospital Nano Reg Med Ltd

There is a high clinical demand for new smart biomaterials, which stimulate neuronal cell proliferation, migration and increase cell-material interaction to facilitate nerve regeneration across these critical-sized defects. This article briefly reviews several up-to-date published studies using Arginine-Glycine-Aspartic acid peptide sequence, nanocomposite based on polyhedral oligomeric silsesquioxane nanoparticle and nanofibrous scaffolds as promising strategies to enhance peripheral nerve regeneration by influencing cellular behaviour such as attachment, spreading and proliferation. The aim is to establish the potent manipulations, which are simple and easy to employ in the clinical conditions for nerve regeneration and repair.

关键词： nanomaterial regenerative medicine biomaterial peptides nerve regeneration stem cells nanotechnology

Linking, thinking & fixing:the story about the 2015 Chemistry Nobel Prize and the future of science

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Science Bulletin 2015年第23期60卷 2071-2076页

作者： Huan Meng Olivia J.Osborne Department of medicine University of California Los Angeles centre for Environmental Implications of nanotechnology University of California Los Angeles

Did you know there are roughly over 10,000 DNA damages in a cell per day?Do not be too alarmed though,thanks to various vital DNA repair mechanisms we are able to continue function normally as an organism and maintain our genome *** is no wonder then why this year prestigious Chemistry Nobel Prize(s)went to *** Lindahl,Aziz Sancar,and Paul Modrich for their

关键词：诺贝尔化学奖诺贝尔奖连接故事科学脱氧核糖核酸修复机制基因组

The role of YAP in the control of the metastatic potential of oral cancer

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Oncology Research 2021年第6期29卷 377-391页

作者： USAMA SHARIF AHMAD KARTHIK SARAVANAN HONG WAN centre for Oral Immunobiology and regenerative medicine Institute of DentistryBarts and the LondonSchool of Medicine and DentistryLondonE12ATUK

The Yes-associated protein(YAP)is a downstream effector of the Hippo pathway and acts as a key transcription co-factor to regulate cell migration,proliferation,and *** Hippo pathway is evolutionarily conserved and controls tissue growth and organ *** and heterogeneity of this pathway are found in cancers,including oral squamous cell carcinoma(OSCC),leading to overexpression of YAP and its regulated proliferation *** activity of YAP is associated with its nuclear expression and is negatively regulated by the Hippo kinase-mediated phosphorylation resulting in an induction of its cytoplasmic *** review focuses on the role of YAP in OSCC in the context of cancer metastatic potential and highlights the latestﬁndings about the heterogeneity of YAP expression and its nuclear transcription activity in oral cancer cell *** review also discusses the potential target of YAP in oral cancer therapy and the recentﬁnding of the unprecedented role of the desmosomal cadherin desmoglein-3(DSG3)in regulating Hippo-YAP signaling.

关键词： Review YAP Phospho-YAP Desmoglein-3 TAZ Hippo pathway desmosomes OSCC Oral cancer cell migration

Lessons from zebrafish on reprogramming the epigenetic ：ode after fertilisation

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Asian Journal of Andrology 2013年第5期15卷 582-583页

作者： Chris O'Neill centre for Developmental and regenerative medicine Kolling Institute for Medical Research Sydney MedicalSchool University of Sydney NSW 2065 Australia

Methylation of cytosine bases encodes epigenetic information that governs lineage-specific patterns of gene expression. Remodelling the methylated genome accompanies major transitions during embryo development, including fertilisation, blastulation and gastrulation. The first whole genome epigenetic maps of this modification across these embryonic transitions in a metazoan has been reported in the zebrafish,

关键词： Lessons zebrafish fertilisation

Neuroinflammation and comorbidities are frequently ignored factors in CNS pathology

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Neural Regeneration Research 2015年第9期10卷 1349-1355页

作者： Raluca Elena Sandu Ana Maria Buga Adriana Uzoni Eugen Bogdan Petcu Aurel Popa-Wagner Center of Clinical and Experimental Research University of Medicine and Pharmacy Craiova Department of Psychiatry University of Medicine Rostock Biochemistry Department University of Medicine and Pharmacy "Victor Babes" Timisoara Griffith University School of medicine Regenerative Medicine Centre

Virtually all drug interventions that have been successful pre-clinically in experimental stroke have failed to prove their efficacy in a dinical setting. This could be partly explained by the complexity and heterogeneity of human diseases as well as the associated co-morbidities which may render neuroprotective drugs less efficacious in dinical practice. One aspect of crucial importance in the physiopathology of stroke which is not completely understood is neuroinflammation. At the pres- ent time, it is becoming evident that subtle, but continuous neuroinflammation can provide the ground for disorders such as cerebral small vessel disease. Moreover, advanced aging and a number of highly prevalent risk factors such as obesity, hypertension, diabetes and atherosclerosis could act as ＂silent contributors＂ promoting a chronic proinflammatory state. This could aggravate the out- come of various pathological entities and can contribute to a number of subsequent post-stroke complications such as dementia, depression and neurodegeneration creating a pathological vicious cycle. Moreover, recent data suggests that the inflammatory process might be dosely linked with multiple neurodegenerative pathways related to depression. In addition, pro-inflammatory cyto- kines could play a central role in the pathophysiology of both depression and dementia.

关键词： aging stroke neuroinflammation comorbidities depression dementia

Modeling the gene delivery process of the needle array-based tissue nanotransfection

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Nano Research 2022年第4期15卷 3409-3421页

作者： Zhigang Li Yi Xuan Subhadip Ghatak Poornachander RGuda Sashwati Roy Chandan K.Sen Indiana Center for regenerative medicine and Engineering Indiana University Health Comprehensive Wound CenterDepartment of SurgeryIndiana University School of MedicineIndianapolisIN 46202USA Birck nanotechnology Center and Weldon School of Biomedical Engineering Purdue UniversityWest LafayetteIN 47907USA

Hollow needle array-based tissue nanotransfection(TNT)presents an in vivo transfection approach that directly translocate exogeneous genes to target tissues by using electric *** this work,the gene delivery process of TNT was simulated and experimentally *** adopted the asymptotic method and cell-array-based model to investigate the electroporation behaviors of cells within the skin *** distribution of nonuniform electric field across the skin results in various electroporation behavior for each *** underneath the hollow microchannels of the needle exhibited the highest total pore numbers compared to others due to the stronger localized electric *** percentage of electroporated cells within the skin structure,with pore radius over 10 nm,increases from 25%to 82%as the applied voltage increases from 100 to 150 V/***,the gene delivery behavior across the skin tissue was investigated through the multilayer-stack-based *** delivery distance increased nonlinearly as the applied voltage and pulse number increased,which mainly depends on the diffusion characteristics and electric conductivity of each *** was also found that the skin is required to be exfoliated prior to the TNT procedure to enhance the delivery *** work provides the foundation for transition from the study of murine skin to translation use in large animals and human settings.

关键词： tissue nanotransfection silicon needle array hollow needle array electroporation electrophoresis gene delivery

Modular operation of microfluidic chips for highly parallelized cell culture and liquid dosing via a fluidic circuit board

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Microsystems & Nanoengineering 2020年第1期6卷 106-121页

作者： A.R.Vollertsen D.de Boer S.Dekker B.A.M.Wesselink R.Haverkate H.S.Rho R.J.Boom M.Skolimowski M.Blom R.Passier A.van den Berg A.D.van der Meer M.Odijk BIOS Lab on Chip Group MESA+Institute for NanotechnologyUniversity of TwenteEnschedeThe Netherlands Mesoscale Chemical Systems MESA+Institute for NanotechnologyUniversity of TwenteEnschedeThe Netherlands Institute for Technology-Inspired regenerative medicine Maastricht UniversityMaastrichtThe Netherlands Micronit Microtechnologies EnschedeThe Netherlands Applied Stem Cell Technologies TechMed CentreUniversity of TwenteEnschedeThe Netherlands

Microfluidic systems enable automated and highly parallelized cell culture with low volumes and defined liquid *** achieve this,systems typically integrate all functions into a single,monolithic device as a“one size fits all”***,this approach limits the end users’(re)design flexibility and complicates the addition of new functions to the *** address this challenge,we propose and demonstrate a modular and standardized plug-and-play fluidic circuit board(FCB)for operating microfluidic building blocks(MFBBs),whereby both the FCB and the MFBBs contain integrated valves.A single FCB can parallelize up to three MFBBs of the same design or operate MFBBs with entirely different *** operation of the MFBBs through the FCB is fully automated and does not incur the cost of an extra external *** use this modular platform to control three microfluidic large-scale integration(mLSI)MFBBs,each of which features 64 microchambers suitable for cell culturing with high spatiotemporal *** show as a proof of principle that we can culture human umbilical vein endothelial cells(HUVECs)for multiple days in the chambers of this ***,we also use the same FCB to control an MFBB for liquid dosing with a high dynamic *** results demonstrate that MFBBs with different designs can be controlled and combined on a single *** novel modular approach to operating an automated microfluidic system for parallelized cell culture will enable greater experimental flexibility and facilitate the cooperation of different chips from different labs.

关键词： FCB operation culture