Modeling the gene delivery process of the needle array-based tissue nanotransfection

作     者：Zhigang Li Yi Xuan Subhadip Ghatak Poornachander RGuda Sashwati Roy Chandan K.Sen Zhigang Li;Yi Xuan;Subhadip Ghatak;Poomachander R.Guda;Sashwati Roy;Chandan K.Sen

作者机构：Indiana Center for Regenerative Medicine and EngineeringIndiana University Health Comprehensive Wound CenterDepartment of SurgeryIndiana University School of MedicineIndianapolisIN 46202USA Birck Nanotechnology Center and Weldon School of Biomedical EngineeringPurdue UniversityWest LafayetteIN 47907USA 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2022年第15卷第4期

页      面：3409-3421页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：This work was supported in part by National Institutes of Health(NIH)grant(No.DK128845) Department of Defense grant(Nos.W81XWH-21-1-0097,W81XWH-21-1-0033,and W81XWH-20-1-251)to C.K.S,NIH grant(No.GM143572)to Y.X,and NIH grant(No.DK129592)to S.G 

主　　题：tissue nanotransfection silicon needle array hollow needle array electroporation electrophoresis gene delivery 

摘      要：Hollow needle array-based tissue nanotransfection(TNT)presents an in vivo transfection approach that directly translocate exogeneous genes to target tissues by using electric *** this work,the gene delivery process of TNT was simulated and experimentally *** adopted the asymptotic method and cell-array-based model to investigate the electroporation behaviors of cells within the skin *** distribution of nonuniform electric field across the skin results in various electroporation behavior for each *** underneath the hollow microchannels of the needle exhibited the highest total pore numbers compared to others due to the stronger localized electric *** percentage of electroporated cells within the skin structure,with pore radius over 10 nm,increases from 25%to 82%as the applied voltage increases from 100 to 150 V/***,the gene delivery behavior across the skin tissue was investigated through the multilayer-stack-based *** delivery distance increased nonlinearly as the applied voltage and pulse number increased,which mainly depends on the diffusion characteristics and electric conductivity of each *** was also found that the skin is required to be exfoliated prior to the TNT procedure to enhance the delivery *** work provides the foundation for transition from the study of murine skin to translation use in large animals and human settings.