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大鼠海马神经干细胞的扩增及与三维微小凹图式复合的研究

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第三军医大学学报 2010年第6期32卷 520-524页

作者：吕艳玲吴泽志张利光宋兆全于婷文灿阴金波陈景龙重庆大学生物工程学院生物流变科学与技术教育部重点实验室重庆400044 第三军医大学基础医学部外科应用解剖与手术学教研室重庆400038 第三军医大学新桥医院神经外科全军癫痫病诊治中心重庆400037 division of Molecular biosciences The John Curtin School of Medical ResearchThe Australian National University

目的比较2种培养基下海马神经干细胞(neural stem cells,NSCs)的生长特性,进而实现海马NSCs扩增的优化及其与聚乳酸(poly-L-lactide,PLLA)三维微小凹图式的复合。方法分离大鼠胚胎海马细胞并采用Neurobasal为基础的培养基和dMEM/F12为... 详细信息

目的比较2种培养基下海马神经干细胞(neural stem cells,NSCs)的生长特性,进而实现海马NSCs扩增的优化及其与聚乳酸(poly-L-lactide,PLLA)三维微小凹图式的复合。方法分离大鼠胚胎海马细胞并采用Neurobasal为基础的培养基和dMEM/F12为基础的培养基进行扩增。以四甲基氮唑蓝(MTT)比色法及神经球数目统计法评价2种培养基下细胞增殖行为。采用紫外光光刻、硅蚀刻及软光刻技术制备PLLA三维微小凹图式并实现海马NSCs与微小凹图式的复合。结果原代分离的海马细胞呈神经干细胞标志物阳性并能向神经元系和胶质细胞系分化。在30d的扩增时间内,海马NSCs在以Neurobasal为基础的培养基中缓慢扩增聚集成神经球,少见细胞贴壁及分化;在以dMEM/F12为基础的培养基中海马NSCs扩增迅速,但易贴壁和分化。培养第25天时后者神经球数量为前者的4.7倍。微加工制备的微小凹图式结构清晰、稳定,具有高纵横结构比(≥1)。扩增的海马NSCs能在三维微小凹图式上成功复合生长。结论以dMEM/F12为基础的培养基有利于大鼠海马NSCs的扩增,以Neurobasal为基础的培养基利于海马NSCs的纯化。序贯应用2种培养基可有效扩增海马NSCs并实现其与三维微小凹图式的复合。

关键词：海马神经干细胞神经球原代培养扩增聚乳酸微加工

Anticarin-β shows a promising antiosteosarcoma effect by specifically inhibiting CCT4 to impair proteostasis

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2268-2279页

作者： Gan Wang Min Zhang Ping Meng Chengbo Long Xiaodong Luo Xingwei Yang Yunfei Wang Zhiye Zhang James Mwangi Peter Muiruri Kamau Zhi daic Zunfu Ke Yi Zhang Wenlin Chen Xudong Zhao Fei Ge Qiumin Lv Mingqiang Rong dongsheng Li Yang Jin Xia Sheng Ren Lai Key Laboratory of Animal Models and Human disease Mechanisms Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan ProvinceKIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesNational Resource Center for Non-Human PrimatesKunming Primate Research Centerand National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyKunming 650107China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Institutes for drug discovery and development Chinese Academy of SciencesShanghai 201203China Sino-African Joint Research Center Chinese Academy of ScienceWuhan 430074China department of Pathology First Afﬁliated HospitalSun Yat-sen UniversityGuangzhou 510080China department of Orthopaedic Surgery First Afﬁliated Hospital of Zhengzhou UniversityZhengzhou 450052China department of Breast Surgery Third Afﬁliated Hospital of Kunming Medical UniversityYunnan Cancer CenterKunming 650118China department of Breast Surgery First Afﬁliated Hospital of Kunming Medical UniversityKunming 650032China department of biosciences University of OsloOslo 0316Norway Key Laboratory of Environment and Health Ministry of Education&Ministry of Environmental ProtectionAnd State Key Laboratory of Environmental HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 434000China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan 430071China

Unlike healthy, non-transformed cells, the proteostasis network of cancer cells is taxed to produce proteins involved in tumor development. Cancer cells have a higher dependency on molecular chaperones to maintain proteostasis. The chaperonin T-complex protein ring complex(TRiC) contains eight paralogous subunits(CCT1-8), and assists the folding of as many as 10% of cytosolic *** is essential for the progression of some cancers, but the roles of TRiC subunits in osteosarcoma remain to be explored. Here, we show that CCT4/TRiC is significantly correlated in human osteosarcoma,and plays a critical role in osteosarcoma cell survival. We identify a compound anticarin-β that can specifically bind to and inhibit CCT4. Anticarin-β shows higher selectivity in cancer cells than in normal cells. Mechanistically, anticarin-β potently impedes CCT4-mediated STAT3 maturation. Anticarin-β displays remarkable antitumor efficacy in orthotopic and patient-derived xenograft models of ***, our data uncover a key role of CCT4 in osteosarcoma, and propose a promising treatment strategy for osteosarcoma by disrupting CCT4 and proteostasis.

关键词： Proteostasis CCT TRiC Osteosarcoma STAT3 Anticarin-β PdX model

Current knowledge of COVId-19:Advances,challenges and future perspectives

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Biosafety and Health 2021年第4期3卷 202-209页

作者： Yuhan Wu Zhuobing Peng Yongxue Yan Jintao Hu Yalong Wang Xiaoyu Wang Ruchao Peng daniel Watterson Yi Shi College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China MGI BGI-ShenzhenShenzhen 518083China Huitong Experimental Station of Forest Ecology CAS Key Laboratory of Forest Ecology and ManagementInstitute of Applied EcologyShenyang 110164China Melbourne School of Engineering the University of MelbourneParkvilleVIC 3010Australia The State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life ScienceSchool of Life ScienceTsinghua UniversityBeijing 100084China MOE Laboratory of Biosystem Homeostasis and Protection College of Life SciencesZhejiang UniversityHangzhou 310058China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Australian Infectious diseases Research Centre School of Chemistry and Molecular BiosciencesThe University of QueenslandBrisbaneQueensland 4072Australia

The pandemic of coronavirus disease 2019(COVId-19)has already evoked massive *** global pandemic has been ravaging the whole world for a year,with the number of confirmed human infection cases over 150 million and a death toll exceeding 3 *** the genomic sequence of the cognate pathogen SARS-CoV-2(severe acute respiratory syndrome coronavirus 2)has been quickly determined,there are still many unknown aspects,including the virus origin and evolution trend,and the effectiveness of current vaccines and drugs against the mutating *** review summarizes current knowledge and advances about COVId-19,including virus origin,transmission and infection,with the aim to improve the understanding of COVId-19 and provide a new perspective for future studies.

关键词： COVId‐19 SARS‐CoV‐2 Virus origin Transmission Virus infection

Meeting report:a hard look at the state of enamel research

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International Journal of Oral Science 2017年第4期9卷 193-199页

作者： ophir d klein olivier duverger wendy shaw rodrigo s lacruz derk joester janet moradian-oldak megan k pugach j timothy wright sarah e millar ashok b kulkarni john d bartlett thomas gh diekwisch pamela den besten james p simmer program in craniofacial biology and department of orofacial sciences university of californiasan franciscoSan FranciscoUSA department of pediatrics and institute for human genetics university of californiasan franciscoSan FranciscoUSA laboratory of skin biology national institute of arthritis and muscular and skin diseasesnational institutes of healthBethesdaUSA physical science division pacific northwest national laboratoriesRichlandUSA department of basic science and craniofacial biology new york university college of dentistryNewYorkUSA department of materials science and engineering northwestern universityevanstonusaEvanstonUSA center for craniofacial molecular biology herman ostrow school of dentistryuniversity of southern californiaLos AngelesUSA the forsyth institute CambridgeUSA department of developmental biology harvard school of dental medicineBostonUSA department of pediatric dentistry university of north caroline school of dentistryChapel HillUSA departments of dermatology and cel&developmental biology perelman school of medicineand department of anatomy&cell biologyschool of dental medicineuniversity of pennsylvaniaPhiladelphiaUSA functional genomics section national institute of dental and craniofacial researchBethesdaUSA division of biosciences ohio state universitycollege of dentistryColumbusUSA department of periodontics and center for craniofacial research and diagnosis texas a&m university college of dentistryDallasUSA center for children’s oral health research and department of orofacial sciences university of californiasan franciscoSan FranciscoUSA biologic and materials sciences and division of prosthodontics university of michiganAnn ArborUSA

The Encouraging Novel Amelogenesis Models and Ex vivo cell Lines （ENAMEL） development workshop was held on 23 June 2017 at the Bethesda headquarters of the National institute of dental and Craniofacial Research （NIdCR）. discussion topics included model organisms, stem cells/cell lines, and tissues/3d cell culture/organoids. Scientists from a number of disciplines, representing institutions from across the United States, gathered to discuss advances in our understanding of enamel, as well as future directions for the field.

关键词： enamel mineralized tissue mineralization ameloblast stem cell

Cerebral dopamine neurotrophic factor transfection in dopamine neurons using neurotensin-polyplex nanoparticles reverses 6-hydroxydopamine-induced nigrostriatal neurodegeneration

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Neural Regeneration Research 2022年第4期17卷 854-866页

作者： Manuel A.Fernandez-Parrilla david Reyes-Corona Yazmin M.Flores-Martinez Rasajna Nadella Michael J.Bannon Lourdes Escobedo Minerva Maldonado-Berny Jaime Santoyo-Salazar Luis O.Soto-Rojas Claudia Luna-Herrera Jose Ayala-davila Juan A.Gonzalez-Barrios Gonzalo Flores Maria E.Gutierrez-Castillo Armando JEspadas-Alvarez Irma A.Martínez-dávila Porfirio Nava daniel Martinez-Fong departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa Institucional de Biomedicina Molecular Escuela Nacional de Medicina y HomeopatíaInstituto Politécnico NacionalCiudad de MéxicoMéxico department of biosciences IIIT-SrikakulamRajiv Gandhi University of Knowledge Technologies(RGUKT)Andhra PradeshIndia department of Pharmacology Wayne State University School of MedicineDetroitMIUSA departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de MéxicoTlalnepantla de BazEdo.de MéxicoMéxico departamento de Fisiología Escuela Nacional de Ciencias BiológicasCiudad de MéxicoMéxico Laboratorio de Medicina Genómica Hospital Regional“1°de Octubre”ISSSTECiudad de MéxicoMéxico Laboratorio de Neuropsiquiatría Instituto de FisiologíaBenemérita Universidad Autónoma de PueblaPueblaPueblaMéxico departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Programa de nanociencias y nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Overexpression of neurotrophic factors in nigral dopamine neurons is a promising approach to reverse neurodegeneration of the nigrostriatal dopamine system,a hallmark in Parkinson's *** human cerebral dopamine neurotrophic factor(h CdNF)has recently emerged as a strong candidate for Parkinson's disease *** study shows that h CdNF expression in dopamine neurons using the neurotensinpolyplex nanoparticle system reverses 6-hydroxydopamine-induced morphological,biochemical,and behavioral *** independent electron microscopy techniques showed that the neurotensin-polyplex nanoparticles containing the h CdNF gene,ranging in size from 20 to 150 nm,enabled the expression of a secretable h CdNF in *** injection in the substantia nigra compacta on day 21 after the 6-hydroxydopamine lesion resulted in detectable h CdNF in dopamine neurons,whose levels remained constant throughout the study in the substantia nigra compacta and *** with the lesioned group,tyrosine hydroxylase-positive(TH^(+))nigral cell population and TH+fiber density rose in the substantia nigra compacta and striatum after h CdNF *** increase inβIII-tubulin and growth-associated protein 43 phospho-S41(GAP43 p)followed TH^(+)cell recovery,as well as dopamine and its catabolite *** reversal(80%)of drugactivated circling behavior and full recovery of spontaneous motor and non-motor behavior were ***-derived neurotrophic factor recovery in dopamine neurons that also occurred suggests its participation in the neurotrophic *** findings support the potential of nanoparticle-mediated h CdNF gene delivery to develop a disease-modifying treatment against Parkinson's *** Institutional Animal Care and Use Committee of Centro de Investigación y de Estudios Avanzados approved our experimental procedures for animal use(authorization No.162-15)on June 9,2019.

关键词： axonal growth brain-derived neurotrophic factor gene delivery nanoparticles neuritogenesis neuronal cytoskeleton neuroregeneration neurorestoration neurotrophic therapy Parkinson's disease reinnervation substantia nigra

Corrigendum to'Cancer chemotherapy and beyond:Current status,drug candidates,associated risks and progress in targeted therapeutics'[Genes&diseases 10(2023)1367-1401]

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Genes & diseases 2024年第4期11卷 426-427页

作者： Uttpal Anand Abhijit dey Arvind K.Singh Chandel Rupa Sanyal Amarnath Mishra devendra Kumar Pandey Valentina de Falco Arun Upadhyay Ramesh Kandimalla Anupama Chaudhary Jaspreet Kaur dhanjal Saikat dewanjee Jayalakshmi Vallamkondu Jose M.Perez de la Lastra department of Life Sciences Ben-Gurion University of the NegevBeer-Sheva 84105Israel department of Life Sciences Presidency UniversityKolkataWest Bengal 700073India Center for disease Biology and Integrative Medicine Faculty of MedicineThe University of TokyoTokyo 113-0033Japan department of Botany Bhairab Ganguly College(affliated to West Bengal State University)KolkataWest Bengal 700056India Faculty of Science and Technology Amity Institute of Forensic SciencesAmity University Uttar PradeshNoida 201313India department of Biotechnology School of Bioengineering and BiosciencesLovely Professional UniversityPhagwaraPunjab 144411India Institute of Endocrinology and Experimental Oncology(IEOS) National Research Council(CNR)Department of Molecular Medicine and Medical Biotechnology(DMMBM)University of Naples FedericoⅡNaples 80131Italy department of Biochemistry School of Life SciencesCentral University of RajasthanBandar SindariKishangarhAjmerRajasthan 305817India CSiR-indian Institute of Chemical Technology HyderabadTelangana 500007India department of Biochemistry Kakatiya Medical CollegeWarangalTelangana 506007India Orinin-BioSystems LE-52Lotus Road 4CHD CityKarnalHaryana 132001India department of Computational Biology Indraprastha Institute of Information Technology Delhi(ⅢT-D)Okhla Industrial EstatePhaseⅢNew Delhi 110020India Advanced Pharmacognosy Research Laboratory Department of Pharmaceutical TechnologyJadavpur UniversityKolkata 700032India department of Physics National Institute of Technology-WarangalWarangalTelangana 506004India Biotechnology of Macromolecules Research Group Instituto de Productos Naturales y AgrobiologiaIPNA-CSICSan Cristobal de La Laguna 38206TenerifeSpain

In the published article,there was an error in the affiliation information about the first author Uttpal Anand[a].Instead of"department of Life Sciences,Ben-Gurion University of the Negev,Beer-Sheva 84105,Israel",it should be"CytoGene Research&development LLP,K-51,UPSIdA Industrial Area,Kursi Road(Lucknow),***,225001,Uttar Pradesh,India".The authors would like to apologize for any inconvenience caused and state that this does not change the scientific conclusions of thearticle inanyway.

关键词： Cancer Cor targeted

Attention drawing of Movie Trailers Revealed by Electroencephography Using Sample Entropy

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Journal of biosciences and Medicines 2014年第4期2卷 6-11页

作者： Po-Shan Wang Shang-Ran Huang Chao-Wen Tsai Chia-Feng Lu Shin Teng C.-I. Hung Yu-Te Wu 2department of Biomedical Imaging and Radiological Sciences National Yang-Ming University Taipei Chinese Taipei 3Institute of Biophotonics National Yang-Ming University Taipei Chinese Taipei department of Biomedical Imaging and Radiological Sciences National Yang-Ming University Taipei Chinese Taipei The Neurological Institute Taipei Municipal Gan-Dau Hospital New Taipei Chinese Taipei

A movie trailer is a common advertising tool in the entertainment industry. detection of a viewer’s brain responses to a movie trailer can help film producers to tailor a more appealing trailer of a movie. In this study, we acquired electroencephalographic (EEG) signals from subjects when they watched movie trailers (labeled as Movie session), and compared with their resting state session (labeled as Resting session) or when they watch nature scenes (labeled as Nature session). We used Sample Entropy (SampEn) to analyze the EEG signals between different sessions. Results showed that the complexity ratios at Fp1, Fp2 and Fz channels derived from Movie session were significantly lower than that in Resting state or when subjects watched Nature session (p d from the change of EEG.

关键词： Neuromarketing Electroencephalography Sample Entropy Movie Trailer

More than a Few LAB Alleviate Common Allergies: Impact of Paraprobiotics in Comparison to Probiotical Live Cells

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Journal of biosciences and Medicines 2014年第3期2卷 56-64页

作者： Nditange Shigwedha Lanwei Zhang Liubov Sichel Li Jia Pimin Gong Wenli Liu Shumei Wang Shuang Zhang Xue Han Wei Gao 1School of Food Science and Engineering Harbin Institute of Technology Harbin China 2Department of Food Science and Technology University of Namibia Windhoek Namibia 3Pure Research Products LLC Boulder USA Pure Research Products LLC Boulder USA School of Food Science and Engineering Harbin Institute of Technology Harbin China Wyeth Nutrition Suzhou China

The evidence in this paper indicates that the alarming increase of common allergies can be reduced by the intake of particular “probiotics” or “paraprobiotics” along with food. This appears to build a consensus in the pharmaceutical and food communities about the role of probiotics in the prevention and treatments of common allergies. Food allergy is one of the common allergies, defined as an adverse health effect arising from a specific immune response that occurs reproducibly on exposure to a given food. Improving the digestion of foods and maintaining a healthy gastrointestinal (GI) tract is certainly critical to controlling food allergens. Therefore, the association between a leaky gut or an impaired intestinal permeability and food-allergenic reactions is explained. Gluten has been found to be somehow a justification for protein allergy, and should, therefore, be avoided by people with celiac disease. In several, in vitro models, surface layer (S- layer) proteins of selective paraprobiotics have shown potential in alleviating food allergies and intestinal disorders. Notably, lactobacilli paraprobiotics have proven to be the immediate immunomodulators against common allergies and other diseases, including viral (flu, hepatitis C), bacterial (bronchitis), asthma, chronic fatigue, fibromyalgia, gastrointestinal distress, and autism disorders in humans.

关键词： Paraprobiotics Food Allergy S-Layer Proteins Immunoglobulin E (IgE) Probiotical Cell Lysates Lactic Acid Bacteria (LAB)

dietary and Biochemical Profile of Congolese Athletes in Endurance Races during International Competition

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Journal of biosciences and Medicines 2022年第9期10卷 340-357页

作者： Eddie Janvier Bouhika Alain Marc Boussana Paul Roger Mabounda Kounga Florent Nsompi Simplice Innocent Moussouami Burton Calvin Mboutou denove Styven Lolo Rhyne Andrée Bouhoyi Pambou Yvon Simplice Itoua-Okouango Michel Elenga François Mbemba Unité de Recherche Nutrition Santé et Motricité Humaine Institut Supérieur d’Education Physique et Sportive Université Marien Ngouabi Brazzaville République du Congo Unité de Recherche Education Santé Expertise et Optimisation de la Performance Motrice Université Marien Ngouabi Brazzaville République du Congo Laboratoire de Physiologie de l’Effort et de Biomécanique (LPB) Institut Supérieur d’Education physique et Sportive (ISEPS) Université Marien Ngouabi Brazzaville République du Congo Laboratoire de l’alimentation et Nutrition Humaine (LANH) Faculté des Sciences et Techniques Université Marien Ngouabi Brazzaville République du Congo

Biochemical parameters are useful in the diagnosis of many health abnormalities in athletes. Some studies suggest training well and eating a balanced diet to improve performance. The modification of biochemical parameters during endurance races has not yet been studied in many African countries. Our objective was to evaluate the dietary profile and the effects of competition on the biochemical parameters of Congolese endurance athletes. The method used was the questionnaire for the food profile and blood samples were taken to measure the biochemical parameters of the athletes. A total of 64 athletes, i.e. 20 girls and 44 boys divided into two groups (group 1 or experimental group (EG), 32 endurance athletes and group 2 or control group (CG), 32 walkers who participated in a cross-sectional study in Brazzaville. The subjects were respectively 26.16 ± 2.79 years old for the EG and 27.44 ± 3.34 years old for the CG. The results indicated that quality foods were difficult to access. The main course was more consumed compared to the starter and dessert (60% of girls and 63.63 of boys). However, biochemical parameters showed a significantly lower serum creatinine concentration in EG subjects compared to CG subjects (0.86 ± 0.06 mg/dl vs 1.04 ± 0.16 mg/dl;t = ?5.95;p 0.05). Similarly, Total Cholesterol was significantly low (1.66 ± 0.34 g/l vs 2.09 ± 0.50 g/l;t = ?3.99;p dL-C) were significantly elevated (0.98 ± 0.49 g/l vs 0.48 ± 0.18 g/l;t = 5.34;p data were normal but the food profile of the runners was unfavorable, inadequate and unbalanced. This is considered to be a performance limiting factor.

关键词： Food Profile Biochemical Parameters Congolese Athletes Endurance Race International Competition