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Factors influencing communication of traditional Chinese medicine use between patients and doctors:A multisite cross-sectional study

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Journal of Integrative medicine 2019年第6期17卷 396-403页

作者： Wen Qiang Lee Jeremy Teoh Pei Zheng Kenneth Lee Zhi Xiong Gerard Low Xueling Sim Foong Fong Mary Chong Norbert Ludwig Wagner yong loo lin school of medicine National University of Singapore117597Singapore Saw Swee Hock school of Public Health National University of Singapore117549Singapore

Objective: In Singapore, the use of traditional Chinese medicine(TCM) alongside Western medicine(WM)is common. There are risks of adverse herb-drug interactions when taken concurrently. Current literature suggests that TCM use is not regularly reported to WM doctors in Singapore, but the underlying reasons are not ***: A cross-sectional study was conducted across Singapore by administering questionnaires to TCM-using patients and WM-practising general practitioners(GPs). The questionnaire examined the following themes:(1) demographics and TCM use pattern;(2) respondents'(patients and GPs) knowledge and beliefs about TCM and the factors influencing the discussion of TCM during the WM consultation;and(3) respondents' qualitative suggestions to increase disclosure ***: A total of 484 patients and 334 GPs were surveyed. Factors associated with patients' initiation of TCM discussion include length of consultation(odds ratio [OR]: 2.1;P < 0.001), comfort level in discussing TCM(OR: 1.6;P < 0.001) and belief in importance of discussion(OR: 1.4;P = 0.017). Doctor's initiation of discussion(74%) was the top patient-ranked factor influencing their discussion of TCM. For doctors,knowledge of TCM indications(OR: 2.2;P < 0.001), belief in importance of discussion(OR: 2.1;P < 0.001) and comfort level in discussing TCM(OR: 1.9;P = 0.001) were associated with their initiation of TCM use discussion. Possible WM-TCM interactions(58%) was the top doctor-ranked factor influencing their discussion of ***: The discussion of TCM in a WM setting is multifactorial. Interventions include doctors' active screening for TCM use in patients and equipping doctors with TCM knowledge. Improving communication between patients and doctors is key to avoiding harmful herb-drug interactions.

关键词： Traditional Chinese medicine Western medicine Patient-provider communication Primary care General practice Public health

Development of a C3c-based ELISA method for the determination of anti-complementary potency of Bupleurum polysaccharides

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Acta Pharmaceutica Sinica B 2015年第4期5卷 316-322页

作者： Mulu Wu Hong Li Yunyi Zhang Daofeng Chen Department of Pharmacology School of Pharmacy Fudan University Department of Microbiology Yong Loo Lin School of Medicine National University of Singapore Department of Pharmacognosy School of Pharmacy Fudan University

Traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. However, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. The main objective of our study was to develop a specific enzyme-linked immunosorbent assay(ELISA) as an alternative method to assess the anti-complement activity, particularly against the lectin pathway. By using respective coating substrates against different activation pathways, followed by capturing the stable C3c fragments, our ELISA method can be used to screen complement inhibitors against the classical pathway and the lectin pathway. The inhibitory effect of suramin on the classical pathway, as measured by our hemolytic assay is consistent with previous reports. Further assessment of suramin and Bupleurum polysaccharides against the lectin pathway showed a good reproducibility of the method. Comparison of the lectin pathway IC50 between Bupleurum smithii var. parvifolium polysaccharides(1.055 mg/mL) and Bupleurum chinense polysaccharides(0.98 mg/mL) showed that, similar to the classical and alterative pathway, these two Bupleurum polysaccharides had comparable anti-complementary properties against the lectin pathway. The results demonstrate that the described ELISA assay can compensate for the shortcomings of the hemolytic assay in lectin pathway.

关键词： ELISA Complement C3c Suramin Bupleurum smithii var parvifolium Bupleurum chinense Polysaccharides

Mechano-responsive hydrogel for direct stem cell manufacturing to therapy

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Bioactive Materials 2023年第6期24卷 387-400页

作者： Yufeng Shou ling Liu Qimin Liu Zhicheng Le Khang Leng Lee Hua Li Xianlei Li Dion Zhanyun Koh Yuwen Wang Tong Ming Liu Zheng Yang Chwee Teck Lim Christine Cheung Andy Tay Department of Biomedical Engineering National University of Singapore117583Singapore Institute for Health Innovation&Technology National University of Singapore117599Singapore school of Civil Engineering and Architecture Wuhan University of Technology430070WuhanChina nus Tissue Engineering Program National University of Singapore117510Singapore Lee Kong Chian school of medicine Nanyang Technological University636921Singapore school of Mechanical and Aerospace Engineering Nanyang Technological University639798Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Mechanobiology Institute National University of Singapore117411Singapore

Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory *** cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in *** overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to *** our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and *** addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK *** our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.

关键词： Mesenchymal stem cell Dynamic mechanical stimulation Magnetic hydrogel Stem cell manufacturing Cell therapy

Npac Is A Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 110-128页

作者： Sue Yu Jia Li Guanxu Ji Zhen Long Ng Jiamin Siew Wan Ning Lo Ying Ye Yuan Yuan Chew Yun Chau Long Wensheng Zhang Ernesto Guccione Yuin Han Loh Zhi-Hong Jiang Henry Yang Qiang Wu Department of Biochemistry Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Cancer Science Institute of Singapore Centre for Translational MedicineSingapore 117599Singapore The State Key Laboratory of Quality Research in Chinese medicine Macao University of Science and TechnologyMacao Special Administrative Region 999078China Cam-Su Genomic Resource Center Soochow UniversitySuzhou 215123China Institute of Molecular and Cell Biology Singapore 138673Singapore

Chromatin modification contributes to pluripotency maintenance in embryonic stem cells(ESCs).However,the related mechanisms remain ***,we show that Npac,a"reader"of histone H3 lysine 36 trimethylation(H3K36me3),is required to maintain mouse ESC(mESC)pluripotency since knockdown of Npac causes mESC *** of Npac in mouse embryonic fibroblasts(MEFs)inhibits reprogramming ***,our chromatin immunoprecipitation followed by sequencing(ChIP-seq)results of Npac reveal that Npac co-localizes with histone H3K36me3 in gene bodies of actively transcribed genes in ***,we find that Npac interacts with positive transcription elongation factor b(p-TEFb),Ser2-phosphorylated RNA PolⅡ(RNA PolⅡSer2P),and Ser5-phosphorylated RNA PolⅡ(RNA PolⅡSer5 P).Furthermore,depletion of Npac disrupts transcriptional elongation of the pluripotency genes Nanog and *** together,we propose that Npac is essential for the transcriptional elongation of pluripotency genes by recruiting p-TEFb and interacting with RNA PolⅡSer2P and Ser5P.

关键词： Npac Pluripotency Reprogramming Histone H3K36me3 Transcriptional elongation

Polo-like kinase 1,a new therapeutic target in hepatocellular carcinoma

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World Journal of Gastroenterology 2012年第27期18卷 3527-3536页

作者： Wei Chuen Mok Shanthi Wasser Theresa Tan Seng Gee Lim Institute of Molecular and Cell Biology Agency for ScienceTechnology and ResearchSingapore 138673Singapore Department of Biochemistry Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Department of Gastroenterology and Hepatology Yong Loo Lin School of MedicineNational University Health SystemSingapore 117597Singapore Department of medicine National University of SingaporeSingapore 119074Singapore

AIM:To investigate the role of polo-like kinase 1 (PLK1) as a therapeutic target for hepatocellular carcinoma (HCC).METHODS:PLK1 gene expression was evaluated in HCC tissue and HCC cell *** knockdown with short-interfering RNA (siRNA) was used to study PLK1 gene and protein expression using real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting,and cell proliferation using 3-(4,5-dim ethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2(4-sulf ophenyl)-2H-tetrazolium (MTS) and bromodeoxyuridine (BrdU) *** was evaluated using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay,and caspase-inhibition ***-7 cells were transplanted into nude mice and co-cultured with PLK1 siRNA or control siRNA,and tumor progression was compared with ***:RT-PCR showed that PLK1 was overexpressed 12-fold in tumor samples compared with controls,and also was overexpressed in Huh-7 *** against PLK1 showed a reduction in PLK1 gene and protein expression of up to 96% in Huh-7 cells,and a reduction in cell proliferation by 68% and 92% in MTS and BrdU cell proliferation assays,*** was a 3-fold increase in apoptosis events,and TUNEL staining and caspase-3 assays suggested that this was *** pan-caspase inhibitor Z-VAD-FMK was unable to rescue the apoptotic *** co-localized endonuclease-G to fragmented chromosomes,implicating it in ***-7 cells transplanted subcutaneously into nude mice showed tumor regression in siPLK1-treated mice,but not in ***:Knockdown of PLK1 overexpression in HCC was shown to be a potential therapeutic target,leading to apoptosis through the endonuclease-G pathway.

关键词：肝细胞肝癌治疗靶点 caspase-3 激酶 Western印迹逆转录聚合酶链反应 RT-PCR

Anti-malarial drug,artesunate,as a novel therapeutic drug target for airway wall remodeling in asthma

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中国药理学与毒理学杂志 2015年第S1期29卷 54-55页

作者： SherylTAN BenjaminONG ChangCHENG WanxingEugeneHO JohnK.CTAM AlastairG.STEWART TrudiHARRIS Wai-ShiuFredWONG ThaiTRAN Department of Physiology yong loo lin school of medicine National University of Singapore Department of Pharmacology yong loo lin school of medicine National University of Singapore Saw Swee Hock school of Public Health National University Health System Singapore Department of Surgery yong loo lin school of medicine National University of Singapore Singapore Department of Pharmacology University of Melbourne Australia

OBJECTIVE Airway wall remodeling(AWR),which refers to structural changes in the airway,is a key characteristic of *** smooth muscle(ASM)cell hypertrophy and hyperplasia contributes to ***,which are used as first line therapy for the treatment of asthma,reduce ASM proliferation but the magnitude of their anti-proliferative actions is dependent on the mitogen ***,glucocorticoid therapy is accompanied by many side ***,a semi-synthetic artemisinin derivative,has been widely used to treat malaria with minimal *** has been shown to attenuate allergic airway inflammation in ***,its role in treating AWR in asthma is not *** this study,we hypothesize that artesunate has anti-proliferative actions on ASM cells,potentially reversing *** and RESULTS Quiescent primary human ASM cells were pre-treated(1h)with artesunate(3,10,30μmol·L-1)before being stimulated with either FBS(10%)or thrombin(0.3U·mL-1).Following 48 h stimulation with mitogen,cells were counted using a ***(Dex,100nmol·L-1)was used as a positive *** concentration-dependently reduced cell number and the magnitude of inhibition appeared to be non-mitogen ***,we examined the effect of artesunate on two important signalling proteins involved in cell proliferation,ERK1/2phosphorylation and cyclin D1 protein *** reduced cyclin D1 protein levels significantly following 20 h stimulation with either thrombin or FBS but had no effect on ERK1/2 phosphorylation following 6h ***,artesunate(30μmol·L-1),but not Dex,inhibited the phosphorylation of Akt,which is upstream of cyclin ***,we show that the inhibitory effect of artesunate,but not Dex,on ASM cell number is retained at least 24h post-treatment following stimulation with *** an acute murine model of allergic asthma,artesunate treatment decreased sm-α-actin positive cells and cyclin D1 protein

关键词： asthma airway wall remodeling PI3K cyclin D1

Dicoumarol is an effective post-exposure prophylactic for SARS-CoV-2 Omicron infection in human airway epithelium

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3455-3465页

作者： Yang Peng Shi-ying Chen Zhao-ni Wang Zi-qing Zhou Jing Sun Gui-an Zhang Jia Li Lei Wang Jin-cun Zhao Xiao Xiao Tang De-Yun Wang Nan-shan Zhong State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhouGuangdongChina Department of Otolaryngology Infectious Diseases Translational Research ProgramYong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore Guangzhou Laboratory GuangzhouChina

Repurposing existing drugs to inhibit SARS-CoV-2 infection in airway epithelial cells(AECs)is a quick way to find novel treatments for *** screening has found dicoumarol(DCM),a natural anticoagulant,to be a potential SARS-CoV-2 inhibitor,but its inhibitory effects and possible working mechanisms remain *** air-liquid interface culture of primary human AECs.

关键词： Omi infection epithelium

Factors early in life associated with hepatic steatosis

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World Journal of Hepatology 2022年第6期14卷 1235-1247页

作者： Sabrina Xin Zi Quek Eunice Xiang-Xuan Tan Yi Ping Ren Mark Muthiah Evelyn Xiu ling loo Elizabeth Huiwen Tham Kewin Tien Ho Siah Division of Gastroenterology and Hepatology Department of MedicineNational University HospitalSingapore 119228Singapore National University Centre for Organ Transplantation National University Health SystemSingapore 119228Singapore yong loo lin school of medicine National University of SingaporeSingapore 117597Singapore Department of medicine National University HospitalSingapore 119228Singapore Department of Paediatrics Yong Loo Lin School of MedicineNational University of SingaporeKhoo Teck Puat-National University Children's Medical InstituteNational University HospitalNational University Health SystemSingapore 119228Singapore Singapore Institute for Clinical Sciences Agency for ScienceTechnology and ResearchSingapore 117609Singapore

BACKGROUND The rise in prevalence of non-alcoholic fatty liver disease(NAFLD)mirrors the obesity *** is insidious but may gradually progress from simple steatosis to steatohepatitis,fibrosis and cirrhosis and/or hepatocellular *** strategies to ameliorate developmental programming of NAFLD may be more efficacious during critical windows of developmental *** To review the early developmental factors associated with *** Databases MEDlinE via PubMed,and EMBASE and Reference Citation Analysis were searched and relevant publications up to April 30,2021 were *** research studies that included risk factors associated with early development of NAFLD in human subjects were *** factors include:Maternal factors,intrauterine and prenatal factors,post-natal factors,genetic and ethnic predisposition,childhood and adolescence environmental *** were excluded if they were review articles or animal studies,case reports or conference abstracts,or if NAFLD was not clearly defined and assessed *** Of 1530 citations identified by electronic search,420 duplicates were *** the 1110 citations screened from title and abstract,80 articles were included in the final *** polymorphisms such as patatin-like phospholipase domain-containing protein 3(PNPLA3)and membrane-bound O-acyltransferase domain-containing protein 7(MBOAT7)were associated with increased risk of *** factors such as maternal obesogenic environment and parental history of hepatic steatosis was associated with offspring *** duration of exclusive breastfeeding in infancy was associated with a lower risk of developing NAFLD later in life while metabolic dysfunction and/or obesity in adolescence was associated with increased risk of *** relating to socioeconomic factors and its association with NAFLD reported confounding *** Maternal metabolic dysfunction during pr

关键词： Epidemiology Natural history Obesity Fatty liver Developmental

Perioperative steroid administration reduces overall complications in patients undergoing liver resection:A meta-analysis

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World Journal of Gastrointestinal Surgery 2021年第9期13卷 1079-1094页

作者： Hao-Han Hai Phoebe Aw Thomas Zheng Jie Teng Vishal G Shelat nus yong loo lin school of medicine Singapore 119228Singapore NTU Lee Kong Chian school of medicine Singapore 308232Singapore Department of General Surgery Tan Tock Seng HospitalSingapore 308433Singapore

BACKGROUND Hepatic resection(HR)results in an inflammatory response that can be modified by perioperative steroid ***,it remains to be determined if this response's attenuation translates to a reduction in *** To evaluate if perioperative administration of steroids reduces complications following *** A systematic review of randomized controlled trials(RCTs)was conducted on PubMed,Embase,and Cochrane Central Register of Controlled Trials to evaluate the effect of perioperative steroid(compared to placebo or no intervention)use in patients undergoing *** outcomes were extracted,and meta-analysis was *** 8 RCTs including 590 patients were *** steroid administration was associated with significant reduction in postoperative complications[odds ratios:0.58;95%confidence intervals(CI):0.35-0.97,P=0.04].There was also improvement in biochemical and inflammatory markers,including serum bilirubin on postoperative day 1[MD:-0.27;95%CI:(-0.47,-0.06),P=0.01],Creactive protein on postoperative day 3[MD:-4.89;95%CI:(-5.83,-3.95),P<0.001],and interleukin-6 on postoperative day 1[MD:-54.84;95%CI:(-63.91,-45.76),P<0.001].CONCLUSION Perioperative steroids administration in HR may reduce overall complications,postoperative bilirubin,and *** studies are needed to determine the optimal dose and duration and patient selection.

关键词： Steroid Liver resection Outcome Systematic review