Npac Is A Co-factor of Histone H3K36me3 and Regulates Transcriptional Elongation in Mouse Embryonic Stem Cells

作     者：Sue Yu Jia Li Guanxu Ji Zhen Long Ng Jiamin Siew Wan Ning Lo Ying Ye Yuan Yuan Chew Yun Chau Long Wensheng Zhang Ernesto Guccione Yuin Han Loh Zhi-Hong Jiang Henry Yang Qiang Wu Sue Yu;Jia Li;Guanxu Ji;Zhen Long Ng;Jiamin Siew;Wan Ning Lo;Ying Ye;Yuan Yuan Chew;Yun Chau Long;Wensheng Zhang;Ernesto Guccione;Yuin Han Loh;Zhi-Hong Jiang;Henry Yang;Qiang Wu

作者机构：Department of BiochemistryYong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Cancer Science Institute of SingaporeCentre for Translational MedicineSingapore 117599Singapore The State Key Laboratory of Quality Research in Chinese MedicineMacao University of Science and TechnologyMacao Special Administrative Region 999078China Cam-Su Genomic Resource CenterSoochow UniversitySuzhou 215123China Institute of Molecular and Cell BiologySingapore 138673Singapore 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2022年第20卷第1期

页      面：110-128页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by Singapore National Medical Research Council(Grant No.CBRG14nov065) the Macao Science and Technology Development Fund,China(Grant No.FDCT-18-033-SKL-016A) 

主　　题：Npac Pluripotency Reprogramming Histone H3K36me3 Transcriptional elongation 

摘      要：Chromatin modification contributes to pluripotency maintenance in embryonic stem cells(ESCs).However,the related mechanisms remain ***,we show that Npac,areaderof histone H3 lysine 36 trimethylation(H3K36me3),is required to maintain mouse ESC(mESC)pluripotency since knockdown of Npac causes mESC *** of Npac in mouse embryonic fibroblasts(MEFs)inhibits reprogramming ***,our chromatin immunoprecipitation followed by sequencing(ChIP-seq)results of Npac reveal that Npac co-localizes with histone H3K36me3 in gene bodies of actively transcribed genes in ***,we find that Npac interacts with positive transcription elongation factor b(p-TEFb),Ser2-phosphorylated RNA PolⅡ(RNA PolⅡSer2P),and Ser5-phosphorylated RNA PolⅡ(RNA PolⅡSer5 P).Furthermore,depletion of Npac disrupts transcriptional elongation of the pluripotency genes Nanog and *** together,we propose that Npac is essential for the transcriptional elongation of pluripotency genes by recruiting p-TEFb and interacting with RNA PolⅡSer2P and Ser5P.