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Xenoesterogens and male infertility:myth or reality?

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Asian Journal of Andrology 2000年第4期2卷 263-269页

作者： Roya Rozati P.P.Reddy P.Reddanna Rubina Mujtaba Assisted Conception Services Unit Mahavir Hospital and Research Centre Hyderabad(A. P.) 500 028 IndiaInstitute of Genetics and Hospital for Genetic Diseases Hyderabad IndiaDepartment of Animal Sciences School of Life Sciences University of Hyderabad IndiaAssisted Conception Services Unit Mahavir Hospital and Research Centre Hyderabad(A. P.) 500 028 India

Aim: To evaluate the role of polychlorinated biphenyls (PCBs) as a potential environmental hazard in the deteriora-tion of male fertility. Methods: Fifty-three males were studied. After a thorough case history evaluation and rele-vant clinical and laboratory investigations, PCBs were estimated in the seminal plasma of 21 infertile men with 'Unex-plained Male Factor' and 32 fertile controls. Peak retention times of the eluants were compared with those of the com-mercially available standard PCB Mix, and the results confirmed spectrophotometrically. Seminal PCB concentrafonswere compared between i) fertile and infertile men and ii) men from different areas and diets. The relationship betweenPCB concentrations and measures of sperm quality such as the total motile sperm count, was assessed. Results:PCBs were detected in seminal plasma of infertile men but absent from controls. Sperm quantity and quality were sig-nificantly lower in infertile men compared to controls. The highest average PCB concentrations were found in fish-eat-ing urban dwellers, and followed in succession by fish-eating rural dwellers, non fish-eating urban dwellers and nonfish-eating mral dwellers. The total motile sperm counts were inversely proportional to the PCB concentrations and weresignificantly lower than those of the respective controls. Conclusion: PCBs may be instrumental in the deteriorationof sperm quantity and quality, a contaminated fish diet being the main source of exposure.(Asian J Androl 2000 Dec; 2: 263-269 )

关键词： polychlorinated biphenyls xenoestrogens male infertility sperm

Differential RNA Editing of Mitochondrial Genes in WA-Cytoplasmic Based Male Sterile Line Pusa 6A, and Its Maintainer and Restorer Lines

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Rice science 2019年第5期26卷 282-289页

作者： Umakanta NGANGKHAM Swarup Kumar PARIDA Ashok Kumar SINGH Trilochan MOHAPATRA National Research Centre on Plant Biotechnology New Delhi 110012India indian Council of Agricultural Research-Research Complex for North East Hill Region Indian Council of Agricultural ResearchUmiam 793103India National Institute of Plant Genome Research New Delhi 110067India Division of genetics Indian Agricultural Research InstituteNew Delhi 110012India indian Council of Agricultural Research New Delhi 110001India

RNA editing changes the nucleotides at the transcript level of mitochondrial genes which results in synthesis of functional *** study was designed to find the editing sites which could be implicated in male fertility restoration and to develop editing based markers for differentiation of cytoplasmic male sterility and maintainer lines from each *** and RNA from young panicles were isolated from three-line system of hybrid rice PRH10,wild abortive(WA)cytoplasm based male sterile(A line Pusa 6A),maintainer(B line Pusa 6B)and restorer(R line PRR78)*** 6A and PRR78 having the same WA cytoplasm are allo-nuclear and iso-cytpolasmic *** genomic and cDNA amplicons for eight mitochondrial genes(18SrRNA,atp6,atp9,cobII,coxI,coxIII,nadI and rps3)were sequenced and *** in genomic and cDNA sequences were considered as *** hundred and thirty editing sites having base substitution or insertion/deletion were identified with the highest in 18SrRNA(5.74%)and the lowest in coxI(0.60%).The highest editing sites were observed in fertile maintainer Pusa 6B followed by PRR78 and Pusa 6A,of which random five editing sites in five different rice mitochondrial transcripts namely atp9,cobII,coxIII,rps3 and 18SrRNA were chosen and validated through cleaved amplified polymorphism sequence(CAPS)analysis and found to be partially edited in four *** identical editing sites of different mitochondrial genes from maintainer and restorer lines might reflect their possible contribution to fertility restoration of sterile WA cytoplasm.

关键词： cleaved amplified polymorphism sequence cytoplasmic male sterility hybrid rice RNAediting mitochondrial gene wild abortive nucleotide

Genetic linkage mapping and QTL identification for salinity tolerance in indian mustard(Brassica juncea *** and Coss.)using SSR markers

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Oil Crop Science-中国油料作物学报英文版 2023年第3期8卷 191-205页

作者： Rekha Patel Sumit Jangra Ram Avtar Neelam R.Yadav Ram C.Yadav Department of Molecular Biology Biotechnology&BioinformaticsCCS Haryana Agricultural UniversityHisar-125004India Department of genetics and Plant Breeding CCS Haryana Agricultural UniversityHisar125004India

Soil salinity is one of the major environmental constraints that limits crop yield and nearly 7%of the total area worldwide is affected by ***-induced oxidative stress causes membrane damage during germination and seedling *** mustard is a major oilseed crop in india and its production and productivity are severely affected by salt *** Brassica cultivars for salinity tolerance by conventional means is very difficult and ***,understanding the molecular components associated with salt tolerance is needed to facilitate breeding for salt tolerance in *** this investigation,quantitative trait loci(QTLs)associated with salt tolerance were identified using F_(2:3)mapping population developed from a cross between CS52(salinity tolerant)and RH30(salinity sensitive).Parents and F_(2:3)were evaluated under controlled and salinity stress conditions for 14 morpho-physiological traits for two consecutive generations(F2 and F_(2:3)),explaining proportion of the phenotypic variance under control *** sequence repeat(SSR)markers were used for mapping studies.A genetic linkage map based on 42 simple sequence repeats(SSRs)markers was constructed covering 2298.5 cM(Haldane)to identify the loci associated with salt tolerance in Brassica ***-one SSRs showing polymorphism in the parents(CS52 and RH30)were mapped on 8 linkage groups(C1–C8).One marker(nga 129)did not map to any of the linkage group and was excluded from *** group 5(C5;317.9 cM)was longest and linkage group 1(C1,255.0 cM)was ***,we identified 15 QTLs controlling 8 traits using F_(2:3)*** QTLs explained 12.44–60.63%of the phenotypic variation with a LOD score range of 3.62–*** of these QTLs,QMI4.1 related to membrane injury showed 51.28%phenotypic variance with a LOD score of *** QBYP8.1 related to biological yield per plant showed 60.63%phenotypic variance at a LOD score of *** highest LO

关键词： Brassica juncea Salt stress tolerance SSR markers Linkage mapping QTL identification

Understanding Brown Planthopper Resistance in Rice: genetics, Biochemical and Molecular Breeding Approaches

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Rice science 2021年第6期28卷 532-546页

作者： Lakesh MUDULI Sukanta Kumar PRADHAN Abinash MISHRA Debendra Nath BASTIA Kailash Chandra SAMAL Pawan Kumar AGRAWAL Manasi DASH Department of Plant Breeding and genetics College of AgricultureOdisha University ofAgriculture and TechnologyBhubaneswar 751003OdishaIndia Department of Bioinformatics Centre for Post Graduate StudiesOdisha University of Agriculture and TechnologyBhubaneswar 751003OdishaIndia Department of Agricultural Biotechnology College of AgricultureOdisha University of Agriculture and TechnologyBhubaneswar 751003OdishaIndia

Brown planthopper(BPH,Nilaparvata lugens St自I)is the most devastating pest of rice in Asia and causes significant yield loss *** 37 BPH resistance genes have been identified so far in indica,African rice varieties along with wild germplasms such as Oryza officinalis,***,***,***,*** and O,***/QTLs involved in BPH resistance,including Bph1,bph2/BPH26,Bph3,Bph6,bph7,BPH9,Bph12,Bph14,Bph15,Bph17,BPH18,bph19,Bph20,Bph21(t),Bph27,Bph27©Bph28(t),BPH29,QBph3,QBph4,QBph4.2,Bph30,Bph32,Bph33,Bph35 and Bph36,have been fine-mapped by different researchers across the *** majority of genes/QTLs are located on rice chromosomes 1,3,4,6,11 and *** plants respond to BPH attack by releasing various endogenous metabolites like proteinase inhibitors,callose,secondary metabolites(terpenes,alkaloids,flavonoid,etc.)and volatile *** that,hormonal signal pathways mediating(antagonistic/synergistic)resista nee responses in rice have been well ***-assisted breedi ng and genome editi ng techniq ues can also be adopted for improving resista nee to novel BPH biotypes.

关键词： rice brown planthopper resista nee wild germplasm marker-assisted breedi ng genome editing secondary metabolite

Oxidative stress and mitochondrial transfer:A new dimension towards ocular diseases

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Genes & Diseases 2022年第3期9卷 610-637页

作者： Mohana Devi Subramaniam Mahalaxmi Iyer Aswathy P.Nair Dhivya Venkatesan Sinnakaruppan Mathavan Nimmisha Eruppakotte Soumya Kizhakkillach Manoj kumar Chandran Ayan Roy Abilash Valsala Gopalakrishnan Balachandar Vellingiri SN ONGC Department of genetics and Molecular Biology Vision Research FoundationChennai 600006Tamil NaduIndia Department of Zoology Avinashilingam Institute for Home Science and Higher Education for WomenCoimbatore 641043Tamil NaduIndia Human Molecular Cytogenetics and Stem Cell Laboratory Department of Human Genetics and Molecular BiologyBharathiar UniversityCoimbatore 641046Tamil NaduIndia Department of Biotechnology Lovely Professional UniversityPunjab 144411India Department of Biomedical Sciences School of Bio Sciences and Technology(SBST)Vellore Institute of Technology(VIT)Vellore 600127India

Ocular cells like,retinal pigment epithelium(RPE)is a highly specialized pigmented monolayer of post-mitotic cells,which is located in the posterior segment of the eye between neuro sensory retina and vascular *** functions as a selective barrier and nourishes retinal visual *** a result of high-level oxygen consumption of retinal cells,RPE cells are vulnerable to chronic oxidative stress and an increased level of reactive oxygen species(ROS)generated from *** oxidative stress and ROS generation in retinal cells lead to RPE *** sources including mtDNA damage could be an important factor of oxidative stress in *** therapy and mitochondrial transfer studies are emerging fields in ocular disease *** retinal degenerative diseases stem cell-based transplantation methods are developed from basic research to preclinical and clinical *** research contributions of gene and cell therapy would be a new strategy to prevent,treat and cure various ocular *** review focuses on the effect of oxidative stress in ocular cell degeneration and recent translational researches on retinal degenerative diseases to cure blindness.

关键词： Mitochondrial transfer Oxidative stress Retinal pigment epithelium(RPE) RPE Degeneration Stem cell treatment

Genetic evaluation of twenty seed sources of Asparagus racemosus

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Journal of Forestry Research 2011年第1期22卷 39-42页

作者： Parveen A Kumar H. S. Ginwal Division of genetics and Tree Propagation Forest Research Institute PO IPE Kaulagarh Road Dehradun 248 195 Uttarakhand India

A field trial of 20 seed sources of Asparagus racemosus was conducted at the Forest Research Institute, Dehradun, Uttarakhand, india to evaluate their performance of different economic traits. Genotypic variance, phenotypic variance, genotypic coefficient of variance （GCV） and phenotypic coefficient of variance （PCV） for number of shoots, shoot height, shoot weight, number of roots, root length, root diameter and root weight were calculated. Maximum genotypic and phenotypic variance was observed in shoot height among the shoot - related traits and root length among the root - related traits. For the shoot height, genotypic variance, phenotypic variance, genotypic coefficient of variance, phenotypic coefficient of variance were 231.80, 3924.80, 61.26 and 1037.32, respectively, where those of the root length were 9.55, 16.80, 23.46 and 41.27, respectively. The maximum genetic advance and genetic gain were obtained for shoot height among the shoot-related traits and root length among the root-related traits. Index values were developed for all the seed sources based on the four most important traits, and Panthnagar （Uttrakhand）, Jodhpur （Rajasthan）, Dehradun （Uttarakhand）, Chandigarh （Punjab）, Jammu （Jammu and Kashmir） and Solan （Himachal Pradesh）, were promising seed sources for root production.

关键词： Asparagus racemosus genetic advance genetic gain heritability index selection seed sources

Dietary Conjugated Linoleic Acids Arrest Cell Cycle Progression and Prevent Ovarian Cancer Xenografts Growth Suggesting a Trans-10 Cis-12 Isoform Specific Activity

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Journal of Cancer Therapy 2013年第5期4卷 33-42页

作者： Philippe Thuillier Nupur T. Pande Andrea Ghena Shuang Song Yancey Lawrence Vidya Shridhar Yasmine Akkari Tanja Pejovic Susan Olson Cytopathology Lab Legacy Emanuel Hospital Portland USA Knight Cancer Research Institute Oregon Health and Sciences University Portland USA Molecular and Medical genetics Oregon Health and Science University Portland USA Rich Graviss pvt. ltd. India

Therapies for treating ovarian cancer (OvCa) successfully are largely inadequate. Alternative therapies and diet(s) with preventive potential to debilitated onset, and reduced OvCa tumor burden in situ, have not been systematically studied. Preventive role of conjugated linoleic acids (CLAs) has been reported in many other cancers. We report the first systematic in vitro and in vivo study modeling potential preventive mechanism(s) of CLA, an octadecadienolic fatty acid in clear cell OvCa cell line TOV-21G. We demonstrate that a dose and time-dependent down-regulation of cyclin E and A proteins (p 0.05) by CLA (t10,c12) was concomitant with cell cycle arrest of TOV-21G cell lines in S phase. To understand the molecular mechanism underlying CLA (t10,c12) induced S phase arrest, levels of cell cycle regulatory proteins were determined by western blot analyses. Exposure to CLA (t10,c12) increased p21(CIP1/WAF1), and p27(KIP1) protein levels in a time and dose-dependent manner. Interestingly CLA (t10,c12) did not significantly affect protein levels of cyclin-dependent kinase (cdk) 2, and p53, however, hyperphosphorylated form of pRb (p 0.05) was abrogated. Exposure to CLA (c9,t11) indicated a modest increase in p21(CIP1/WAF1) and p27(KIP1) levels, but changes in cyclin A and E levels were statistically insignificant. These results indicate that CLA (t10,c12) mediated p27(KIP1) upregulation and inhibition of hyperphosphorylation of ppRb may be the possible mechanism for the S phase arrest in TOV-21G cell line. Our in vivo data showed that CLA reduced the progression of TOV-21G xenografts by >50%. Together our results provide evidence of CLA exerted preventive effect on OvCa cell and tumor growth. Tumor growth arrest may be resultant from CLA (t10,c12) mediated modulation of cell cycle arrest.

关键词： Dietary Prevention Ovarian Cancer Cell Cycle DNA Damage Tumor Inhibition

Genetic Basis for Mucormycosis Progression in COVID-19 Patients:From Susceptibility to Severity

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Infectious Diseases & Immunity 2024年第2期4卷 86-92页

作者： Jethendra Kumar Muruganantham Ramakrishnan Veerabathiran Human Cytogenetics and Genomics Laboratory Faculty of Allied Health SciencesChettinad Hospital and Research InstituteChettinad Academy of Research and EducationKelambakkamTamilnadu 603103India.

The dynamics of COVID-19 and mucormycosis reveal a complex interplay of genetic factors that influence the susceptibility,severity,and immune ***-19,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),exhibits an increased incidence of mucormycosis,particularly in individuals with comorbidities or corticosteroid *** is a fungal infection that affects the sinuses,orbits,and lungs and demands timely intervention with antifungal medications and surgery because of its life-threatening *** on the genetic underpinnings of this intersection has unveiled key insights into the pathogenicity of *** in genetic tools have exposed virulence factors,such as the CotH protein family and high-affinity iron-uptake *** susceptibility is a pivotal element in identifying individuals at risk of developing COVID-19,facilitating early detection,and allowing for personalized treatment ***9,MIF,and TYK2 are among the genes implicated in COVID-19 severity,emphasizing the intricate relationship between genetic makeup and viral *** genetic landscape extends to viral entry mechanisms,thereby affecting infection *** polymorphisms in genes such as IFNAR2,OAS3,and TYK2 are associated with COVID-19 severity,indicating shared genetic bases between severe and hospitalized *** is genetically predisposed,particularly in immunocompromised *** challenge lies in understanding the genetic factors influencing susceptibility and offering insights into pathogenesis and potential therapeutic *** transplantation adds another layer,increasing susceptibility to infections such as COVID-19 and *** impact of immunosuppression on COVID-19 severity remains elusive,necessitating ongoing research on the immunological *** the challenges posed by emerging SARSCoV-2 variants,the intricate connection between genetic factors and the

关键词： COVID-19 Mucormycosis Pathogenesis genetics basis

Protective effect of Genistein against N-nitrosodiethylamine(NDEA)-induced hepatotoxicity in Swiss albino rats

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Journal of Pharmaceutical Analysis 2015年第1期5卷 51-57页

作者： Fahad Ali Rahul Falaq Naz Smita Jyoti Yasir Hasan Siddique Section of genetics Department of ZoologyFaculty of Life SciencesAligarh Muslim UniversityAligarhUttar Pradesh 202002India

In the present study,we studied the effect of Genistein against the hepatotoxicity induced by N-nitrosodiethylamine（NDEA）.NDEA is present in almost all kinds of food stuff and has been reported to be a *** male rats were exposed to NDEA（0.1 mg/mL） dissolved in drinking water separately and along with 25,50,100 mg/mL of Genistein for 21 *** activities of serum glutamic oxaloacetic transaminase（SGOT）,serum glutamic pyruvic transaminase（SGPT）.alkaline phosphatase（ALP） and lactate dehydrogenase（LDH） were measured in blood *** peroxidation,protein carbonyl content,micronucleus frequency and DNA damage（Comet assay） were performed on rat *** results of the study reveal that the treatment of NDEA along with Genistein showed a significant dose-dependent decrease in the levels of blood serum enzymes i.e.,SGOT,SGPT,ALP and LDH（P〈0.05）.The HE staining of histological sections of the liver also revealed a protective effect of Genistein.A significant dose-dependent reduction in the lipid peroxidation and protein carbonyl content was observed in rats exposed to NDEA（0.1 mg/mL） along with Genistein（P〈0.05）.The results obtained for the comet assay in rat hepatocytes showed a significant dose-dependent decrease in the mean tail length（P〈0.05）.Thus the present study supports the hepatoprotective role of Genistein.

关键词： Genistein N-nitrosodiethylamine Serum enzymes Antigenotoxic Hepatoprotective