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Insight into the antifungal mechanism of Neosartorya fischeri antifungal protein

Protein & Cell 2015年第7期6卷 518-528页

作者： Mate Viragh Annamaria Marton Csaba Vizier Liliana Toth Csaba Vagvolgyi Florentine Marx Laszlo Galgoczy Department of Microbiology Faculty of Science and Informatics University of Szeged Szeged Hungary Institute of Biochemistry Biological Research Centre Hungarian Academy of Sciences Szeged Hungary Division of Molecular Biology Biocenter Innsbruck Medical University Innsbruck Austria

Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still limited due to their not fully clarified mode of action. The aim of this work was to provide a deep insight into the anti-fungal mechanism of Neosartorya fischeri antifungal protein （NFAP）, a novel representative of this protein group. Within a short exposure time to NFAP, reduced cellular metabolism, apoptosis induction, changes in the actin distribution and chitin deposition at the hyphal tip were observed in NFAP-sensitive Aspergillus nidulans. NFAP did show neither a direct membrane disrupting- effect nor uptake by endocytosis. Investigation of A. nidulans signalling mutants revealed that NFAP acti- vates the cAMP/protein kinase A pathway via G-protein signalling which leads to apoptosis and inhibition of polar growth. In contrast, NFAP does not have any in- fluence on the cell wall integrity pathway, but an un- known cell wall integrity pathway-independent mitogen activated protein kinase A-activated target is assumed to be involved in the cell death induction. Taken to- gether, it was concluded that NFAP shows similarities, but also differences in its mode of antifungal action compared to two most investigated NFAP-related pro-teins from Aspergillus giganteus and Penicillium chrysogenum.

关键词： Neosartorya fischeri antifungal protein,Aspergillus nidulans cAMP/Pka signalling Pkc/Mpksignalling antifungal mechanism

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The cis-trans Isomerisation of Homologous 2-Hydroxycycloalkanecarboxylic Acids under Basic Conditions

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Chinese Journal of Chemistry 2006年第12期24卷 1792-1795页

作者： GYARMATI Cs. Zsuzsanna PALINKO lstván BOKROS Attila MARTINEK A. Tamás BERNATH Gábor Institute of Pharmaceutical Chemistry University ofSzeged Szeged POB 121 H-6701 Hungary research group for Heterocyclic Chemistry of the hungarian academy of sciences and university of szeged Szeged POB 121 H-6701 Hungary Department of Organic Chemistry University of Szeged Szeged Ddm tér 8 H.6720 Hungary

The cis→trans isomerisation of homologous 2-hydroxycycloalkanecarboxylic acids in strongly basic aqueous solution was studied starting from the cis isomers. It was found that the cyclopentane, cyclohexane and cycloheptane homologues afforded synthetically useful amounts of the trans acids and the procedure resulted in relatively small quantities of the corresponding olefinic acids. In contrast, the isomerisation of the cis-2-hydroxycyclooctanecarboxylic acid produced roughly equal amounts of the cis and trans isomers and the 1-cyclooctenecarboxylic acid at equilibrium. Molecular modelling with the PM3 semiempirical method of the reactants, products and the intermediates applying explicit water molecules as reaction medium gave a fair estimate for the rate sequence of the idealised （dehydration-free） isomerisation reactions in aqueous base solution.

关键词： geometric isomerisation base catalysis reaction mechanism molecular modelling

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Knowledge explosion for monogenic skin diseases

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World Journal of Dermatology 2015年第1期4卷 44-49页

作者： Nikoletta Nagy Katalin Farkas Lajos Kemény Márta Széll Dermatological research group of the hungarian academy of sciences University of Szeged Department of Dermatology and Allergology University of Szeged Department of Medical Genetics University of Szeged Dermatological research group of the hungarian academy of sciences University of Szeged

During the past few decades, the investigative technologies of molecular biology- especially sequencing-underwent huge advances, leading to the sequencing of the entire human genome, as well as the identification of several candidate genes and the causative geneticvariations that are responsible for monogenic skin diseases. These advances provided a solid basis for subsequent studies elucidating mechanisms of monogenic skin diseases and improving our understanding of common skin diseases. Furthermore, these discoveries also contributed to the development of novel therapeutic modalities for monogenic skin diseases. In this review, we have used the disease spectrum caused by mutations in the CYLD gene- Brooke-Spiegler syndrome, familial cylindromatosis and multiple familial trichoepithelioma type 1- as a model for demonstrating the knowledge explosion for this group of diseases.

关键词： Familial trichoepitheliomatosis Familial cylindromatosis Brooke-Spiegler syndrome Monogenic skin diseases

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Lesions requiring wound management in a central tertiary neonatal intensive care unit

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World Journal of Pediatrics 2017年第2期13卷 165-172页

作者： Angéla Meszes Gyula Tálosi Krisztina Máder Hajnalka Orvos Lajos Kemény Zsanett Renáta Csoma Department of Dermatology and Allergology University of SzegedSzegedHungary Department of Pediatrics University of SzegedSzegedHungary Department of Obstetrics and Gynecology University of SzegedSzegedHungary Department of Dermatology and Allergology University of SzegedSzegedHungary MTA-SZTE Dermatological research group SzegedHungary

Background:Most of the skin disorders that occur in neonatal intensive care units are due in part to the immaturity and vulnerability of the neonatal *** iatrogenic diagnostic and therapeutic procedures are also conducive to iatrogenic *** study was to review the neonates admitted to our neonatal intensive care unit who needed wound management,and to assess the most common skin injuries and wounds,and their ***:Data were extracted from medical records of neonates who needed wound management in our Neonatal Intensive Care Unit between January 31,2012 and January 31,*** about gestational age,sex,birth weight,area of involvement,wound aetiology,and therapy were ***:Among the 211 neonates observed,wound management was required in 10 cases of diaper dermatitis,7 epidermal stripping,6 extravasation injuries,5 pressure ulcers,1 surgical wound and infection,1 thermal burn,and 5 other ***:International guidelines in neonatal wound care practice are not available,and further research concerns are clearly *** and antiseptic agents should be chosen with great care for application to neonates,with particular attention to the prevention of adverse events in this sensitive *** work among dermatologists,neonatologists and nurses is crucial for the successful treatment of neonates.

关键词： epidermal stripping extravasation injury neonatal intensive care unit surgical wound wound care in neonates

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Forest encroachment in Eastern European forest-steppes at a decadal time scale

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Journal of Plant Ecology 2024年第5期17卷 205-216页

作者： LászlóErdős GáborÓnodi Csaba Tölgyesi György Kröel-Dulay Zoltán Bátori Eszter Aradi Péter Török Khanh Vu Ho Indri Puspitasari LászlóKörmöczi Institute of Ecology and Botany HUN-REN Centre for Ecological Research2163 VácrátótHungary Department of Ecology University of Debrecen4032 DebrecenHungary MTA-SZTE‘Momentum’Applied Ecology research group University of Szeged6726 SzegedHungary Department of Ecology University of Szeged6726 SzegedHungary Kiskunság National Park Directorate 6000 KecskemétHungary HUN-REN-DE Functional and Restoration Ecology research group 4032 DebrecenHungary Botanical Garden–Center for Biological Diversity Conservation in Powsin Polish Academy of Sciences02-973 WarszawaPoland Doctoral School of Environmental sciences University of Szeged6720 SzegedHungary Faculty of Natural Resources-Environment Kien Giang University91706 Kien GiangVietnam

In the Eurasian forest-steppe,with increasing aridity,the balance between naturally co-existing forest and grassland patches is expected to shift towards grassland dominance in the long run,although feedback mechanisms and changes in land-use may alter this *** this study,we compared old and recent aerial photographs of hungarian forest-steppes to find out whether and how the forest proportion and the number of forest patches change at the decadal time *** percentage area covered by forest significantly increased in all study *** observed forest encroachment may be a legacy from earlier land-use:due to ceased or reduced grazing pressure,forests are invading grasslands until the potential forest cover allowed by climate and soil is *** number of forest patches significantly increased at one site(Fülöpháza),while it decreased at two sites(Bugac and Orgovány)and showed no significant change at the fourth site(Tázlár).This indicates that forest encroachment can happen at least in two different ways:through the emergence of new forest patches in the grassland,and through the extension and coalescence of already existing forest *** the present work revealed increasing tree cover at a decadal time scale,the dynamic process should be monitored in the future to see how the vegetation reacts to further *** could help devise a conservation strategy,as the woody/non-woody balance has a profound influence on basic ecosystem properties.

关键词： forest-grassland dynamics grazing semi-open ecosystems tree-grass ecosystems woody-herbaceous ecosystems

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What have we learned about non-involved psoriatic skin from large-scale expression studies?

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World Journal of Dermatology 2014年第3期3卷 50-57页

作者： Eszter Szlavicz Kornelia Szabo Zsuzsanna Bata-Csorgo Lajos Kemeny Marta Szell Department of Dermatology and Allergology University of Szeged H-6720 Szeged Hungary Dermatological research group of the hungarian academy of sciences University of Szeged H-6720 Szeged Hungary Department of Medical Genetics the University of Szeged H-6720 Szeged Hungary

Psoriasis is a chronic inflammatory skin disorder; its genetic background has been widely studied in recent decades. Recognition of novel factors contributing to the pathogenesis of this disorder was facilitated by potent molecular biology tools developed during the 1990 s. Large-scale gene expression studies, including differential display and microarray, have been used in experimental dermatology to a great extent; moreover, skin was one of the first organs analyzed using these methods. We performed our first comprehensive gene expression analysis in 2000. With the help of differential display and microarray, we have discovered several novel factors contributing to the inherited susceptibility for psoriasis, including the EDA+ fibronectin splice variant and PRINS. The long non-coding PRINS RNA is expressed at higher levels in non-involved skin compared to healthy and involved psoriatic epidermis and might be a factor contributing cellular stress responses and, specifically, to the development of psoriatic symptoms. This review summarizes the most important results of our large-scale gene expression studies.

关键词： Non-involved psoriatic skin Differential display cDNA microarray EDA+fibronectin isoform PRINS long non-coding RNA mRNA maturation

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Restoration of energy level in the early phase of acute pediatric pancreatitis

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World Journal of Gastroenterology 2017年第6期23卷 957-963页

作者： Dóra Mosztbacher Nelli Farkas Margit Solymár Gabriella Pár Judit Bajor ákos Szucs József Czimmer Katalin Márta Alexandra Mikó Zoltán Rumbus Péter Varjú Péter Hegyi Andrea Párniczky First Department of Pediatrics Semmelweis University Institute for Translational Medicine University of Pécs Institute for Bioanalyses University of Pécs Department of Gastroenterology First Department of MedicineUniversity of Pécs Department of Translational Medicine First Department of MedicineUniversity of Pécs First Department of Surgery Semmelweis University hungarian academy of sciences-university of szeged Momentum Gastroenterology Multidisciplinary Research Group Heim Pál Children’s Hospital

Acute pancreatitis (AP) is a serious inflammatory disease with rising incidence both in the adult and pediatric populations. It has been shown that mitochondrial injury and energy depletion are the earliest intracellular events in the early phase of AP. Moreover, it has been revealed that restoration of intracellular ATP level restores cellular functions and defends the cells from death. We have recently shown in a systematic review and meta-analysis that early enteral feeding is beneficial in adults; however, no reviews are available concerning the effect of early enteral feeding in pediatric AP. In this minireview, our aim was to systematically analyse the literature on the treatmentof acute pediatric pancreatitis. The preferred reporting items for systematic review(PRISMA-P) were followed, and the question was drafted based on participants, intervention, comparison and outcomes: P: patients under the age of twenty-one suffering from acute pancreatitis; I: early enteral nutrition (per os and nasogastric- or nasojejunal tube started within 48 h); C: nil per os therapy; O: length of hospitalization, need for treatment at an intensive care unit, development of severe AP, lung injury (including lung oedema and pleural effusion), white blood cell count and pain score on admission. Altogether, 632 articles (Pub Med: 131; EMBASE: 501) were found. After detailed screening of eligible papers, five of them met inclusion criteria. Only retrospective clinical trials were available. Due to insufficient information from the authors, it was only possible to address length of hospitalization as an outcome of the study. Our mini-meta-analysis showed that early enteral nutrition significantly(SD = 0.806, P = 0.034) decreases length of hospitalization compared with nil per os diet in acute pediatric pancreatitis. In this minireview, we clearly show that early enteral nutrition, started within 24-48 h, is beneficial in acute pediatric pancreatitis. Prospective studies and better pres

关键词： pediatric pancreatitis enteral nutrition nil per os diet ATP restoration length of hospitalization

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A Synonymous Polymorphism of APCDD1 Affects Translation Efficacy and is Associated with Androgenic Alopecia

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Journal of Life sciences 2014年第2期8卷 106-114页

作者： Nikoletta Nagy Katalin Farkas Agnes Kinyo Barbara Fazekas Kornelia Szabo Edit Kollar Balazs Sztano Angela Meszes Dora Beke Lajos Kemeny Laszlo Royo Marta Szell Department of Medical Genetics University of Szeged Szeged 6720 Hungary Department of Dermatology andAllergology University ofSzeged Szeged 6720 Hungary Dermatological research group Hungarian Academy of Sciences Szeged 6720 Hungary Department of Rhino-Laryngology and Head-Neck Surgery University ofSzeged Szeged 6720 Hungary

The APCDDI （adenomatosis polyposis coli down-regulated 1） gene is an inhibitor of the Wnt signaling pathway, and a rare mutation of this gene has been associated with hereditary hypotrichosis simplex. In this study, the authors aimed to investigate whether common APCDD1 gene polymorphisms contribute to the development of androgenic alopecia. Patients （n = 210） with androgenic alopecia and 98 controls were investigated. SNPs （Single nucleotide polymorphisms） in the coding region of the gene were sequenced. A significant difference in genotype distribution was found for the c. 1781C/T, p.L476L SNP （rs3185480） of the APCDD1 gene. This SNP is located in exon 5 and is associated with a 3.5- and a 2.8-fold increase in risk for the development of androgenic alopecia for homozygote （CI 0.933-13.125; nominal regression P = 0.063） and heterozygote （CI 1.086-7.217; nominal regression P = 0.033） carriers, respectively. These data suggest that the rs3185480 polymorphism contributes to the development of androgenic alopecia. Protein expression experiments revealed that the polymorphism is associated with reduced APCDDI protein abundance. This reduction is likely due to altered codon usage for leucine from a preferred codon （CTC） to a rare codon （CTT）, which might influence translation efficiency and, thus, APCDDI protein level.

关键词： Adenomatosis polyposis coli down-regulated 1 gene hereditary hypotrichosis simplex androgenic alopecia,polymorphism synonymous translation efficacy preferable codon.

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Effect of melatonin on the severity of L-arginine-induced experimental acute pancreatitis in rats

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World Journal of Gastroenterology 2006年第2期12卷 251-258页

作者： Annamaria Szabolcs Russel J Reiter Tamas Letoha Peter Hegyi Gabor Papai Ilona Varga Katalin Jarmay Jozsef Kaszaki Reka Sari Zoltan Rakonczay Jr Janos Lonovics Tamas Takacs st Department of Medicine University of SzegedHungary Department of Cellular and Structural Biology University of TexasHealth Science CentreSan AntonioTexasUnited States Institute of Chemistry University of SzegedHungary Biological research Centre Hungarian Academy of SciencesSzegedHungary Biological Isotope Laboratory University of SzegedHungary Institute of Experimental Surgery University of SzegedHungary

AIM： To determine the effect of melatonin pre- and post-treatment on the severity of L-arginine （L-Arg） -induced experimental pancreatitis in rats. METHODS： Male Wistar rats （25） were divided into five groups. Those in group A received two injections of 3.2 g/kg body weight L-Arg i.p. at an interval of 1 h. In group MA, the rats were treated with 50 mg/kg body weight melatonin i.p. 30 min prior to L-Arg administration. In group AM, the rats received the same dose of melatonin 1 h after L-Arg was given. In group M, a single dose of melatonin was administered as described previously. In group C the control animals received physiological saline injections i.p. All rats were exsanguinated 24 h after the second L-Arg injection. RESULTS： L-Arg administration caused severe necrotizing pancreatitis confirmed by the significant elevations in the serum amylase level, the pancreatic weight/body weight ratio （pw/bw）, the pancreatic IL-6 content and the myeloperoxidase activity, relative to the control values. Elevation of the serum amylase level was significantly reduced in rats given melatonin following L-Arg compared to rats injected with L-Arg only. The activities of the pancreatic antioxidant enzymes （Cu/Zn-superoxide dismutase （CulZn-SOD） and catalase （CAT）） were significantly increased 24 h after pancreatitis induction. Melatonin given in advance of L-Arg significantly reduced the pancreatic CAT activity relative to that in the rats treated with L-Arg alone. In the liver, L-Arg significantly increased the lipid peroxidation level, and the glutathione peroxidase and Cu/Zn-SOD activities, whereas the Mn-SOD activity was reduced as compared to the control rats. Melatonin pre-treatment prevented these changes. CONCLUSION： Melatonin is an antioxidant that is able to counteract some of the L-Arg-induced changes during acute pancreatitis, and may therefore be helpful in the supportive therapy of patients with acute necrotizing pancreatitis.

关键词： Acute pancreatitis Melatonin Scavengers

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MULTIPLE SCLEROSIS AFTER HEPATITIS B VACCINATION IN A 16-YEAR-OLD PATIENT

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Chinese Medical Journal 2006年第1期119卷 77-79页

作者： DANIELLA TERNEY SáNDOR BENICZKY PéTER BARSI ISTVáN KONDáKOR JóZSEF PERéNYI BéLA FALUDI MAGDOLNA SZAPPER LáSZLó VéCSEI DEPARTMENT OF neurology ALBERT SZENT-GYRGYI MEDICAL AND PHARMACEUTICAL CENTRE UNIVERSITY OF SZEGED P.O. BOX 427 H-6701 SZEGED HUNGARY DEPARTMENT OF RADIOLOGY OPNI HüVSVLGYI úT 116 H-1021 BUDAPEST HUNGARY DEPARTMENT OF neurology UNIVERSITY OF PéCS RéT U. 2 H-7623 PéCS HUNGARY DEPARTMENT OF neurology OPNI HüVSVLGYI úT 116 H-1021 BUDAPEST HUNGARY neurology research group of the hungarian academy of sciences and university of szeged SEMMELWEIS U. 6 H-6725 SZEGED HUNGARY

Since the first description of multiple sclerosis （MS）, it has been known that relapse of the disease may be triggered by febrile infections. Although the prevention of febrile illness is therefore clearly advantageous for those with MS there is considerable controversy as to whether vaccine should be administered to such individuals. A further subject of debate is the possibility that vaccination （against influenza,

关键词： hepatitis B vaccination encephalomyelitis multiple sclerosis

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