Insight into the antifungal mechanism of Neosartorya fischeri antifungal protein

作     者：Mate Viragh Annamaria Marton Csaba Vizier Liliana Toth Csaba Vagvolgyi Florentine Marx Laszlo Galgoczy 

作者机构：Department of Microbiology Faculty of Science and Informatics University of Szeged Szeged Hungary Institute of Biochemistry Biological Research Centre Hungarian Academy of Sciences Szeged Hungary Division of Molecular Biology Biocenter Innsbruck Medical University Innsbruck Austria 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2015年第6卷第7期

页      面：518-528页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 071010[理学-生物化学与分子生物学] 07[理学] 08[工学] 083201[工学-食品科学] 

基　　金：supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TAMOP ‘National Excellence Program’ supported by the Hungarian Scientific Research Fund (OTKA) 

主　　题：Neosartorya fischeri antifungal protein,Aspergillus nidulans cAMP/Pka signalling Pkc/Mpksignalling antifungal mechanism 

摘      要：Small, cysteine-rich, highly stable antifungal proteins secreted by filamentous Ascomycetes have great po- tential for the development of novel antifungal strate- gies. However, their practical application is still limited due to their not fully clarified mode of action. The aim of this work was to provide a deep insight into the anti-fungal mechanism of Neosartorya fischeri antifungal protein （NFAP）, a novel representative of this protein group. Within a short exposure time to NFAP, reduced cellular metabolism, apoptosis induction, changes in the actin distribution and chitin deposition at the hyphal tip were observed in NFAP-sensitive Aspergillus nidulans. NFAP did show neither a direct membrane disrupting- effect nor uptake by endocytosis. Investigation of A. nidulans signalling mutants revealed that NFAP acti- vates the cAMP/protein kinase A pathway via G-protein signalling which leads to apoptosis and inhibition of polar growth. In contrast, NFAP does not have any in- fluence on the cell wall integrity pathway, but an un- known cell wall integrity pathway-independent mitogen activated protein kinase A-activated target is assumed to be involved in the cell death induction. Taken to- gether, it was concluded that NFAP shows similarities, but also differences in its mode of antifungal action compared to two most investigated NFAP-related pro-teins from Aspergillus giganteus and Penicillium chrysogenum.