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中国毒理学会第六届全国毒理学大会

作者： ZHANG Yong-bin William SALMINEN SHI Qiang YANG Xi Yvonne JONES Paul HOWARD nctr/ora nanotechnology core facility Food and Drug Administration Division of Systems Biology National Center for Toxicological ResearchFood and Drug Administration

＜正＞With the rapid expansion of nanotechnology,there has been great concern on safety issue of the nano-product regulated by U.S Food and Drug Administration（FDA）.Pre-clinical characterization and quality control during the manufacturing of nano-products is fundamental for understanding resulting safety issues during their wide *** regulated products are required to submit basic physio-chemical characterization as part of Investigational New Drug（IND） Applications or New Drug Applications（NDA） submitted to the FDA for *** of gold nanoparticles（Au NPs）, silver nanoparticles（Ag NPs） and single walled carbon nanotubes（SWCNTs） will be discussed as cases *** nanomaterials were characterized using transmission electron microscopy（TEM）,dynamic light scattering（DLS）,Raman,and hyperspectral microscopy and other spectroscopy techniques. We examined their uptake and toxicity in primary rat hepatocytes,rat adrenal pheochromocytoma cells（PC12） or in vivo rodent *** was assessed by examining cell membrane integrity and mitochondrial activity（LDH,XTT and MTT assay） and oxidative stress（DCF assay;reduced glutathione levels;PCR array for ROS responsive gene expression）.Uptake and subcellular localization were monitored using TEM,confocal Raman microscopy and inductively coupled plasma mass spectrometry（ICP-MS） *** Au NPs were taken up by the cells in a time- and size-dependent manner,no cytotoxicity or ROS stress was ***,uptake of SWCNT was cytotoxic and generated ROS in surface coating-dependent *** SWCNT exhibited less potency than uncoated *** the other hand,Ag NPs decreased the body weight and locomotor activities after intravenous exposure in *** studies suggest the surface coating,size and composition play import roles in toxicity of the *** multiple characterization approaches used in this study provided the solid basis to understand the biological resp

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Resolution-enhanced X-ray fluorescence microscopy via deep residual networks

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npj Computational Materials 2023年第1期9卷 1935-1942页

作者： Longlong Wu Seongmin Bak Youngho Shin Yong S.Chu Shinjae Yoo Ian K.Robinson Xiaojing Huang Computational Science Initiative Brookhaven National LaboratoryUptonNY 11973USA Condensed Matter Physics and Materials Science Department Brookhaven National LaboratoryUptonNY 11973USA National Synchrotron Light Source II Brookhaven National LaboratoryUptonNY 11973USA Materials Engineering Research facility Applied Materials DivisionArgonne National LaboratoryLemontIL 60439USA London Centre for nanotechnology University College LondonLondon WC1E 6BTUK

Multimodal hard X-ray scanning probe microscopy has been extensively used to study functional materials providing multiple contrast *** instance,combining ptychography with X-ray fluorescence(XRF)microscopy reveals structural and chemical properties *** ptychography can achieve diffraction-limited spatial resolution,the resolution of XRF is limited by the X-ray probe ***,we develop a machine learning(ML)model to overcome this problem by decoupling the impact of the X-ray probe from the XRF *** enhanced spatial resolution was observed for both simulated and experimental XRF data,showing superior performance over the state-of-the-art scanning XRF method with different nano-sized X-ray *** spatial resolutions were also observed for the accompanying XRF tomography *** this probe profile deconvolution with the proposed ML solution to enhance the spatial resolution of XRF microscopy will be broadly applicable across both functional materials and biological imaging with XRF and other related application areas.

关键词： resolution overcome instance

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Construction of RNA nanotubes

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Nano Research 2019年第8期12卷 1952-1958页

作者： Hui Li Shaoying Wang Zhouxiang Ji Congcong Xu Lyudmila S. Shlyakhtenko Peixuan Guo Center for RNA Nanobiotechnology and Nanomedicine Division of Pharmaceutics and Pharmaceutical ChemistryCollege of Pharmacy/Department of Physiology & Cell BiologyCollege of Medicine/Dorothy M. Davis Heart and Lung Research Institute and James Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA UNMC Nanoimaging core facility Department of Pharmaceutical SciencesCollege of Pharmacy University of Nebraska Medical CenterOmahaNE68182USA Present address: University of California San FranciscoCA94158USA Present address: P&Z Biological Technology NewarkNJ07103USA

Nanotubes are miniature materials with significant potential applications in nanotechnological, medical, biological and material sciences. The quest for manufacturing methods of nano-mechanical modules is in progress. For example, the application of carbon nanotubes has been extensively investigated due to the precise width control, but the precise length control remains challenging. Here we report two approaches for the one-pot self-assembly of RNA nanotubes. For the first approach, six RNA strands were used to assemble the nanotube by forming a 11 nm long hollow channel with the inner diameter of 1.7 nm and the outside diameter of 6.3 nm. For the second approach, six RNA strands were designed to hybridize with their neighboring strands by complementary base pairing and formed a nanotube with a six-helix hollow channel similar to the nanotube assembled by the first approach. The fabricated RNA nanotubes were characterized by gel electrophoresis and atomic force microscopy (AFM), confirming the formation of nanotube-shaped RNA nanostructures. Cholesterol molecules were introduced into RNA nanotubes to facilitate their incorporation into lipid bilayer. Incubation of RNA nanotube complex with the free-standing lipid bilayer membrane under applied voltage led to discrete current signatures. Addition of peptides into the sensing chamber revealed discrete steps of current blockage. Polyarginine peptides with different lengths can be detected by current signatures, suggesting that the RNA-cholesterol complex holds the promise of achieving single molecule sensing of peptides.

关键词： RNA nanotechnology peptide sensing RNA nanotube nanobiotechnology

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3D RNA nanocage for encapsulation and shielding of hydrophobic biomolecules to improve the in vivo biodistribution

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Nano Research 2020年第12期13卷 3241-3247页

作者： Congcong Xu Kaiming Zhang Hongran Yin Zhefeng Li Alexey Krasnoslobodtsev Zhen Zheng Zhouxiang Ji Sijin Guo Shanshan Li Wah Chiu Peixuan Guo Center for RNA Nanobiotechnology and Nanomedicine College of PharmacyDivision of Pharmaceutics and Pharmaceutical ChemistryCollege of MedicineDorothy M.Davis Heart and Lung Research InstituteJames Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA Department of Bioengineering James H.Clark CenterStanford UniversityStanfordCA94305USA Department of Physics University of Nebraska at OmahaOmahaNE68182USA Nanoimaging core facility Office of Vice-Chancellor for ResearchUniversity of Nebraska Medical CenterOmahaNE68198USA SLAC National Accelerator Laboratory Stanford UniversityMenlo ParkCA94025USA

Ribonucleic acid(RNA)nanotechnology platforms have the potential of harboring therapeutics for in vivo delivery in disease ***,the nonspecific interaction between the harbored hydrophobic drugs and cells or other components before reaching the diseased site has been an obstacle in drug *** we report an encapsulation strategy to prevent such nonspecific hydrophobic interactions in vitro and in vivo based on a self-assembled three-dimensional(3D)RNA *** placing an RNA three-way junction(3WJ)in the cavity of the nanocage,the conjugated hydrophobic molecules were specifically positioned within the nanocage,preventing their exposure to the biological *** assembly of the nanocages was characterized by native polyacrylamide gel electrophoresis(PAGE),atomic force microscopy(AFM),and cryogenic electron microscopy(cryo-EM)*** stealth effect of the nanocage for hydrophobic molecules in vitro was evaluated by gel electrophoresis,flow cytometry,and confocal *** in vivo sheathing effect of the nanocage for hydrophobic molecules was assessed by biodistribution profiling in *** RNA nanocages with hydrophobic biomolecules underwent faster clearance in liver and spleen in comparison to their ***,this encapsulation strategy holds promise for in vivo delivery of hydrophobic drugs for disease treatment.

关键词： ribonucleic acid(RNA)nanocage three-way junction(3WJ) encapsulation hydrophobic biomolecule RNA nanotechnology

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Large-scale synthesis of N-doped carbon capsules supporting atomically dispersed iron for efficient oxygen reduction reaction electrocatalysis

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eScience 2022年第2期2卷 227-234页

作者： Hui Yang Yanfang Liu Xiaolu Liu Xiangke Wang He Tian Geoffrey I.N.Waterhouse Paul E.Krugere Shane GTelfer Shengqian Ma Department of Chemistry University of North TexasDentonTX76201United States College of Environmental Science and Engineering North China Electric Power UniversityBeijing102206PR China State Key Laboratory of Silicon Materials Center of Electron MicroscopySchool of Materials Science and EngineeringZhejiang UniversityHangzhou310027PR China MacDiarmid Institute for Advanced Materials and nanotechnology School of Chemical SciencesThe University of AucklandAuckland1142New Zealand MacDiarmid Institute for Advanced Materials and nanotechnology School of Physical and Chemical SciencesUniversity of CanterburyChristchurch8140New Zealand MacDiarmid Institute for Advanced Materials and nanotechnology School of Fundamental SciencesMassey UniversityPalmerston North4442New Zealand

The large-scale synthesis of platinum-free electrocatalysts for the oxygen reduction reaction(ORR)remains a grand *** report the large-scale production of stable and active ORR electrocatalysts based on iron,an earth-abundant element.A core–shell zeolitic imidazolate framework–tannic acid coordination polymer composite(ZIF-8@K-TA)was utilized as the catalyst precursor,which was transformed into iron atoms dispersed in hollow porous nitrogen-doped carbon capsules(H-Fe-N_(x)-C)through ion exchange and pyrolysis.H-Fe-N_(x)-C fea-tures site-isolated single-atom iron centers coordinated to nitrogen in graphitic layers,high levels of nitrogen doping,and high permeability to incoming *** from these characteristics,H-Fe-N_(x)-C demonstrated efficient electrocatalytic activity(E_(1/2)=0.92 V,***)and stability towards the ORR in both alkaline and acidic *** ORR performance,it surpassed the majority of recently reported Fe-N-C catalysts and the standard Pt/C *** addition,H-Fe-N_(x)-C showed outstanding tolerance to methanol.

关键词： Metal single atoms Hollow carbon capsules Oxygen reduction reaction Metal–organic framework Electrocatalysis

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Microbial resistance to nanotechnologies:An important but understudied consideration using antimicrobial nanotechnologies in orthopaedic implants

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Bioactive Materials 2022年第10期7卷 249-270页

作者： Zhuoran Wu Brian Chan Jessalyn Low Justin Jang Hann Chu Hwee Weng Dennis Hey Andy Tay Institute of Health Innovation&Technology National University of Singapore117599Singapore Department of Biomedical Engineering National University of Singapore117583Singapore Biosafety Level 3 core facility Yong Loo Lin School of MedicineNational University of Singapore117599Singapore Department of Microbiology and Immunology Yong Loo Lin School of MedicineNational University of Singapore117545Singapore Infectious Disease Programme Yong Loo Lin School of MedicineNational University of Singapore117547Singapore Institute of Molecular and Cell Biology 35 Agency for ScienceTechnology and Research138673Singapore National University Health System National University of Singapore119228Singapore Tissue Engineering Programme National University of Singapore117510Singapore

Microbial resistance to current antibiotics therapies is a major cause of implant failure and adverse clinical outcomes in orthopaedic *** developments in advanced antimicrobial nanotechnologies provide numerous opportunities to effective remove resistant bacteria and prevent resistance from occurring through unique *** tunable physicochemical properties,nanomaterials can be designed to be bactericidal,antifouling,immunomodulating,and capable of delivering antibacterial compounds to the infection region with spatiotemporal *** its substantial advancement,an important,but under-explored area,is potential microbial resistance to nanomaterials and how this can impact the clinical use of antimicrobial *** review aims to provide a better understanding of nanomaterial-associated microbial resistance to accelerate bench-to-bedside translations of emerging nanotechnologies for effective control of implant associated infections.

关键词： Bacterial infection Orthopaedic implant Microbial resistance Nanomaterial Antimicrobial nanotechnology

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Construction of hierarchical FeNi_(3)@(Fe,Ni)S_(2) core-shell heterojunctions for advanced oxygen evolution

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Nano Research 2021年第11期14卷 4220-4226页

作者： Minglei Yan Zhiyang Zhao Peixin Cui Kun Mao Chi Chen Xizhang Wang Qiang Wu Hui Yang Lijun Yang Zheng Hu Key Laboratory of Mesoscopic Chemistry of MOE and Jiangsu Provincial Laboratory of nanotechnology School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil Sciencethe Chinese Academy of SciencesNanjing210008China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai201210China

The investigation of earth-abundant electrocatalysts for efficient water electrolysis is of central importance in renewable energy system, which is currently impeded by the large overpotential of oxygen evolution reaction (OER). NiFe sulfides show promising OER activity but are troubled by their low intrinsic conductivities. Herein, we demonstrate the construction of the porous core-shell heterojunctions of FeNi3@(Fe,Ni)S_(2) with tunable shell thickness via the reduction of hierarchical NiFe(OH)x nanosheets followed by a partial sulfidization. The conductive FeNi3 core provides the highway for electron transport, and the (Fe,Ni)S_(2) shell offers the exposed surface for in situ generation of S-doped NiFe-oxyhydroxides with high intrinsic OER activity, which is supported by the combined experimental and theoretical studies. In addition, the porous hierarchical morphology favors the electrolyte access and O_(2) liberation. Consequently, the optimized catalyst achieves an excellent OER performance with a low overpotential of 288 mV at 100 mA·cm^(−2), a small Tafel slope of 48 mV·dec^(−1), and a high OER durability for at least 1,200 h at 200 mA·cm^(−2). This study provides an effective way to explore the advanced earth-abundant OER electrocatalysts by constructing the heterojunctions between metal and corresponding metal-compounds via the convenient post treatment, such as nitridation and sulfidization.

关键词： oxygen evolution reaction electrocatalysts Ni-Fe nitrides core-shell structure heterojunctions

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Ultrastable Near-Infrared Aggregation-Induced Emission Nanoparticles as a Fluorescent Probe:Long-Term Tumor Monitoring and Lipid Droplet Tracking

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CCS Chemistry 2021年第6期3卷 1596-1606页

作者： Haijun Ma Danning Hu Jiajia Zhao Mei Tian Jinying Yuan Yen Wei MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Department of ChemistryTsinghua UniversityBeijing 100084 Key Laboratory of Organic Optoelectronics and Molecular Engineering Department of ChemistryTsinghua UniversityBeijing 100084 Key Laboratory of Molecular Nanostructure and nanotechnology Institute of ChemistryChinese Academy of SciencesBeijing 100190 Department of Nuclear Medicine and Medical PET Center The Second Hospital of Zhejiang University School of MedicineHangzhou 310009 Department of Chemistry Center for NanotechnologyInstitute of Biomedical TechnologyChung Yuan Christian UniversityTaoyuan 32023

Effective real-time tumor monitoring and cell tracking are of great importance for precise diagnosis and therapy of tumors,and also for the surveillance of biological *** this study,a new organic fluorescent nanoprobe(named TPATBT NPs)with unique aggregation-induced emission(AIE)characteristics has been obtained for the first time via facile synthesis to achieve real-time and long-term monitoring in living *** advantages of TPATBT NPs include small size(∼80 nm),a large Stokes shift(∼150 nm),high stability,good dispersibility in aqueous media,and *** addition,such NPs have showed excellent bioimaging performance and unusual long-term tumor monitoring *** red fluorescence signals inside MDA-MB-231 cells last for longer than 10 generations(18 days).Moreover,the cellular uptake of TPATBT NPs has been found to highly rely on energy-dependent endocytosis and clathrin-mediated endocytosis,and to primarily accumulate in lipid droplets(LDs),which can lead to targeted LD cellular imaging and ***,TPATBT NPs can work as an excellent fluorescent nanoprobe for long-term monitoring of malignant tumor growth and dynamic biological processes.

关键词： long term lipid droplets tracking tumor monitoring near infrared aggregation-induced emission

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Templating core-shell particles using metal ion-chelating biosurfactants

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Particuology 2022年第5期20卷 145-152页

作者： Ching-Min Yeh Thomas Jarrett Yuan Gao Chun-Xia Zhao Andrew Whittaker Frank Sainsbury Alison L.White Australian Institute of Bioengineering and nanotechnology The University of QueenslandSt.LuciaQueensland4072Australia ARC Centre of Excellence in Convergent Bio-Nano Science and Technology The University of QueenslandSt Lucia4072Australia CSIRO Probing Biosystems Future Science Platform BrisbaneAustralia Centre for Cell Factories and Biopolymers Griffith Institute for Drug DiscoveryGriffith UniversityBrisbaneQLD4111 Australia

Designer biosurfactants can be used to stabilise and functionalise *** particularly promising use is the stabilisation of oil-in-water emulsions,enabling fine tuning physical,chemical and biological surface *** ability of emulsion systems to carry high payloads makes them attractive for applications in medicine,food and fragrances,and ***,they have limited long-term *** we sought to use the metal ion-chelating ability of the biosurfactant peptide,AM1,to precipitate the formation of a gold metal shell on AM1-stabilised emulsions by electroless *** found that replacing the commonly used zinc(Ⅱ)with palladium(Ⅱ)for coordination by histidine residues of adjacent AM1 peptides produced interfacial films that maintained elasticity at acidic *** NMR suggested a coordination mechanism independent of the imidazole ring of the *** of emulsions at low pH enabled the deposition of a gold shell,albeit by an unexpected *** propose that gold nanoparticles forming in bulk are adsorbed onto the peptide-stabilised interface,accumulating into a particulate *** resulting one-step method for nanoparticle precipitation and shell formation will be useful for the creation of biocompatible core-shell particles for applications where large payloads of hydrophobic active compounds require stability over long time periods.

关键词： Biosurfactants Emulsion core-shell particles Electroless plating Histidine Interfacial elasticity

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Generation of a human embryonic stem cell line stably expressing high levels of the fluorescent protein mCherry

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World Journal of Stem Cells 2012年第7期4卷 71-79页

作者： Dmitry A Ovchinnikov Jennifer P Turner Drew M Titmarsh Nilay Y Thakar Dong Choon Sin Justin J Cooper-White Ernst J Wolvetang Stem Cell Engineering Group Australian Institute for Bioengineering and NanotechnologyUniversity of QueenslandBrisbane 4072QueenslandAustralia Tissue Engineering and Microfluidics Laboratory Australian Institute for Bioengineering and NanotechnologyUniversity of QueenslandBrisbane 4072QueenslandAustralia School of Chemical Engineering University of Queensland Brisbane 4072QueenslandAustralia

AIM:The generation and characterization of a human embryonic stem cell (hESC) line stably expressing red fluorescent mCherry protein. METHODS:Lentiviral transduction of a ubiquitously-expressed human EF-1α promoter driven mCherry transgene was performed in MEL2 hESC. Red fluore-scence was assessed by immunofluorescence and flow cytometry. Pluripotency of stably transduced hESC was determined by immunofluorescent pluripotency marker expression, flow cytometry, teratoma assays andembryoid body-based differentiation followed by reverse transcriptase-polymerase chain reaction. Quantification of cell motility and survival was performed with time lapse microscopy. RESULTS:Constitutively fluorescently-labeled hESCs are useful tools for facile in vitro and in vivo tracking of survival, motility and cell spreading on various surfaces before and after differentiation. Here we describe the generation and characterization of a hESC line (MEL2) stably expressing red fluorescent protein, mCherry. This line was generated by random integration of a fluorescent protein-expressing cassette, driven by the ubiquitously-expressed human EF-1α promoter. Stably transfected MEL2-mCherry hESC were shown to express pluripo-tency markers in the nucleus (POU5F1/OCT4, NANOG and SOX2) and on the cell surface (SSEA4, TRA1-60 and TG30/CD9) and were shown to maintain a normal karyotype in long-term (for at least 35 passages) culture. MEL2-mCherry hESC further readily differentiated into representative cell types of the three germ layers in embryoid body and teratoma based assays and, importantly, maintained robust mCherry expression throughout differentiation. The cell line was next adapted to singlecell passaging, rendering it compatible with numerous bioengineering applications such as measurement of cell motility and cell spreading on various protein modified surfaces, quantification of cell attachment to nanoparticles and rapid estimation of cell survival. CONCLUSION:The MEL2-mCherry hESC line c

关键词： Human embryonic stem cells Fluorescent marker mCherry Pluripotency Cellular motility

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