检索结果-南通市图书馆

Rational confinement engineering of MOF-derived carbonbased electrocatalysts toward CO_(2)reduction and O_(2)reduction reactions

在线全文

学校读者我要写书评

暂无评论

InfoMat 2022年第3期4卷 34-63页

作者： Xiaoyu Zhang Dongping Xue Su Jiang Huicong Xia Yanlin Yang Wenfu Yan Jinsong Hu Jianan Zhang College of Materials Science and Engineering Zhengzhou UniversityZhengzhouP.R.China Academic Publishing Center Henan Polytechnic UniversityJiaozuoP.R.China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry and College of ChemistryJilin UniversityChangchunP.R.China Beijing National Laboratory for molecular sciences(BNLMS) Institute of ChemistryChinese Academy of SciencesBeijingP.R.China

The goal of global carbon peak and neutrality gives an impetus to the utilization of clean energy(e.g.,fuel cell)and carbon dioxide(CO_(2))at a large scale,where the oxygen reduction reaction(ORR)and CO_(2)reduction reaction(CO_(2)RR)are the key reactions via the sustainable system,*** a main precursor for fabricating affordable carbon-based electrocatalysts with uniformly dispersed active centers and tailorable performances for ORR and CO_(2)RR,metal organic frameworks(MOFs)have captured a surge of interest in recent *** the facilitated development of MOF-derived carbon-based electrocatalysts by many investigations,it is still plagued by high overpotential and unsatisfied life span,which are greatly determined by the efficient and alterable confinement effect on synthesis and *** this review,firstly,the confined synthetic strategies(doping engineering,defect engineering,geometric engineering,etc.)of MOF-derived carbon-based electrocatalysts with multi-sized active centers(atom,atomic clusters and nanoparticles(NPs))are systematically summarized;secondly,the confinement effect on the interaction of ORR and CO_(2)RR intermediates,as well as the catalytic durability and activity,was discussed from chemical and physical *** the end,the review discusses the remaining challenges and emerging research topics in the future,including support upgradation and catalyst innovation,high selectivity and effective confinement synthesis,in situ and operando characterization techniques,theoretical investigation,and artificial intelligence(AI)*** new understanding and insights into these aspects will guide the rational confinement concept of MOF-derived carbon-based electrocatalysts for ORR and CO_(2)RR with optimized performances in terms of confinement engineering and are believed to be helpful for filling the existing gaps between scientific communities and practical use.

关键词： carbon-based materials CO_(2)reduction reaction confinement effect electrocatalyst metal organic framework oxygen reduction reaction

Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical sciences Journal 2013年第2期28卷 82-87页

作者： Rui-feng Yang Guo-wei Zhao Shu-ting Liang Hou-zao Chen De-pei Liu National Laboratory of Medical molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College

Objective To investigate the role of lysine-specific demethylase 1 (LSD1) in the process of THP-1 monocyte-to-macrophage *** Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting were performed to analyze the expression of LSD1 and interleukin-6 (IL-6) in THP-1 monocytes and THP-1-derived *** immunoprecipitation (ChIP) assay was applied to detect the occupancy of LSD1 and H3K4 methylation at IL-6 promoter during THP-1 monocyte-to-macrophage ***-6 mRNA level and H3K4 methylation at IL-6 promoter were analyzed using qRT-PCR and ChIP assay in LSD1-knockdown THP-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 0,4,8,12,and 24 *** activated flow cytometry was performed to reveal the percentage of macrophages differentiated from THP-1 *** The expression of LSD1 reduced during THP-1 monocyte-to-macrophage differentiation (P<0.01).LSD1 occupancy decreased and H3K4 methylation increased at IL-6 promoter during the *** knockdown of LSD1,H3K4 methylation at IL-6 promoter was found increased after TPA treatment at different times points (all P<0.05,except 24 hours).The percentage of macrophages increased significantly in the THP-1 cells with LSD1 knockdown (P<0.05).Conclusions LSD1 is repressed during the monocyte-to-macrophage differentiation of THP-1 *** of LSD1-mediated H3K4 demethylation may be required for THP-1 monocyte-to-macrophage differentiation.

关键词：单核细胞巨噬细胞甲基化酶细胞分化赖氨酸异性定量RT-PCR Western印迹

sRAGE alleviates SARS-CoV-2-induced pneumonia in hamster

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第3期7卷 610-612页

作者： Xiuqin Zhang Dan Li Rui Sun Xinli Hu Zhiqi Song Xiaotian Ni Hua Zhu Tiannan Guo Chuan Qin Rui-Ping Xiao Institute of molecular Medicine College of Future TechnologyPeking UniversityBeijingChino Key Laboratory of Human Disease Comparative Medicine of National Health Commission of the People’s Republic of China Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Comparative Medicine CenterPeking Union Medical CollegeBeijingChina Westlake Laboratory of Life sciences and Biomedicine Key Laboratory of Structural Biology of Zhejiang ProvinceSchool of Life SciencesWestlake UniversityHangzhouChina

Dear Editor Since last year,the most demanding task of the global pharmaceutical community has been focused on the development of strategies to treat coronavirus disease 2019(COVID-19).To date,several vaccines have been developed and already demonstrated their efficacy in reducing the incidence of COVID-19.1 However,the development of drugs treating COVID-19 is lagging far behind,and all the current treatment regimens have their limitations.

关键词： drugs regimen pneumonia

Biomimetic nanoparticles directly remodel immunosuppressive microenvironment for boosting glioblastoma immunotherapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第10期7卷 418-432页

作者： Tingting Wang Hao Zhang Weibao Qiu Yaobao Han Hanghang Liu Zhen Li Center for molecular Imaging and Nuclear Medicine State Key Laboratory of Radiation Medicine and ProtectionSchool for Radiological and Interdisciplinary Sciences(RAD-X)Soochow UniversityCollaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsSuzhou215123PR China Paul C.Lauterbur Research Center for Biomedical Imaging Institute of Biomedical and Health EngineeringShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen518055PR China

Glioblastoma(GBM),as a very aggressive cancer of central nervous system,is very challenging to completely cure by the conventional combination of surgical resection with radiotherapy and *** success of emerging immunotherapy in hot tumors has attracted considerable interest for the treatment of GBM,but the unique tumor immunosuppressive microenvironment(TIME)of GBM leads to the failure of ***,we show the significant improvement of the immunotherapy efficacy of GBM by modulating the TIME through novel all-in-one biomimetic nanoparticles(***-I/J@CM NPs).The nanoparticles consist of utrasmall Cu2-xSe nanoparticles(NPs)with outstanding intrinsic properties(e.g.,photo-responsive Fenton-like catalytic property for inducing immunogenic cell death(ICD)and alleviating the hypoxia of tumor),indoximod(IND,an inhibitor of indoleamine-2,3-dioxygenease in tumor),JQ1(an inhibitor for reducing the expression of PD-L1 by tumor cells),and tumor cell membrane for improving the targeting capability and accumulation of nanoparticles in *** reveal that these smart CS-I/J@CM NPs could drastically activate the immune responses through remodeling TIME of GBM by multiple *** could(1)increase M1-phenotype macrophages at tumor site by promoting the polarization of tumor-associated macrophages through the reactive oxygen species(ROS)and oxygen generated from the Fenton-like reaction between nanoparticles and H2O2 within tumor under NIR II irradiation;(2)decrease the infiltration of Tregs cells at tumor site through the release of IND;(3)decrease the expression of PD-L1 on tumor cells through *** notable increments of anti-tumor CD8+T cells in the tumor and memory T cells(TEM)in the spleen show excellent therapy efficacy and effectively prevent the recurrence of GBM after modulation of the *** work demonstrates the modulation of TIME could be a significant strategy to improve the immunotherapy of GBM and other cold tumors.

关键词： Glioblastoma Immunotherapy Ultrasmall copper selenide nanoparticles Tumor immune microenvironment Immune checkpoint blockade therapy

Effect of Hydrophobic Carbon Chain Length on the Crystal Structure of MCM-41

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2008年第4期16卷 631-634页

作者：张光旭陶玲张高勇 School of Chemical Engineering Wuhan University of Technology Wuhan 430072 China College of Chemistry and molecular sciences Wuhan University Wuhan 430072 China

The mesoporous molecular sieve (MCM)-41 using ionic liquid as template has been prepared. The typical template of ionic liquid was [C16mim]X. In this article, the use of 1-alkyl-3-methylimidazolium ([Cnmim]Br, where n=12,14,16,18,20) salts as templates in the synthesis of MCM-41 is reported. The results showed that the synthesized MCM-41 had uniform pore diameter, high surface area and stable framework. The largest surface area of MCM-41 was the one prepared with [C14mim]Br as template. When using [C18min]Br as template, the narrowest pore distribution sample was obtained and the effect of surface tension of template solution to MCM-41 was first discussed.

关键词：离子液体模板剂碳链长度 MCM-41 晶体结构表面张力

Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H 1N 1 virus from peripheral blood memory B lymphocytes

在线全文

学校读者我要写书评

暂无评论

Cellular & molecular Immunology 2013年第5期10卷 403-412页

作者： Hao Wang Chi Ma Yanlai Lu Xu Ji Yongsheng Pang Fang Hua Lianxian Cui Denian Ba Wei He Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking UnionMedical College State Key Laboratory of Medical Molecular Biology Beijing China

The 2009 H 1N 1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H 1N 1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus （EBV）-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies （MAbs） from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition （HAl） activities against the 2009 pandemic H 1N 1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B ceils may also be applicable to other infectious or autoimmune diseases.

关键词： Epstein-Barr virus hemagglutinin memory B cells neutralizing monoclonal antibody 2009 pandemicinfluenza HIN1 virus

Glycoproteomics analysis of plasma proteins associated with Opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2016年第12期9卷 1142-1147页

作者： Pramote Sriwanitchrak Atchara Paemanee Sittiruk Roytrakul Vithoon Viyanant Kesara Na-Bangchang Graduate Program in Biomedical sciences Faculty of Allied Health SciencesThammasat University Proteomics Research Laboratory National Center for Genetic Engineering and Biotechnology Graduate Program in Bioclinical sciences Center of Excellence in Pharmacology and Molecular Biology of Malaria and CholangiocarcinomaChulabhorn International College of Medicine Center of Excellence in Pharmacology and molecular Biology of Malaria and Cholangiocarcinoma Chulabhorn International College of Medicine Excellence Center in Pharmacology and molecular Biology of Malaria and Cholangiocarcinoma Thammasat University

Objective: To apply lectin affinity chromatography and glycoproteomics-based LC-MS/MS to preliminarily investigate the possible potential plasma biomarkers of Opisthorchis viverrini(OV)-associated CCA in OV/dimethylnitrosamine(DMN)-induced CCA hamster model. Methods: Nine Syrian hamsters were divided into 3 groups as follows(n=3 each): normal(healthy control group); OV group; and OV/DMN group(CCA group). Pooled plasma samples collected from animals in each group at the 6th month post-infection with OV metacercarae were subjected to glycoproteomics analysis. Glycoproteins in the pooled sample from each group were initially isolated by concanavilin A(Con A)-based affinity chromatography. The expression of glycoproteins isolated by both enrichment methods were determined using LCMS/MS. Results: Among the 24 Con A-binding glycoproteins isolated, two proteins, N-myc downstream regulated gene 1(NDRG1) and fetuin-B(FETUB) were found up-regulated only in the samples from the OV and control groups, but not in the OV/DMN(CCA) groups. On the other hand, one protein, i.e., NSFL1 cofactor p47 isoform x3(NSFL1C) was found only in the samples from OV/DMN(CCA) and control groups, but not in the OV group. The remaining 21 proteins were upregulated in the samples from all groups. Conclusions: NDRG1, FETUB and NSFL1 C glycoproteins isolated by Con A-based affinity chromatography could be potential biomarkers for CCA. Plasma samples with negative for NDRG1 and FETUB proteins but positive for NSFL1 C are likely to be OV-associated CCA. Nevertheless, this conclusion remains to be confirmed whether this battery test can discriminate OV-associated CCA from other risk factors.

关键词： Cholangiocarcinoma Opisthorchis viverrini Glycoprotein Hamster model ConA binding protein LC-MS/MS

Immediate transcriptional responses of Arabidopsis leaves to heat shock

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of integrative Plant Biology 2021年第3期63卷 468-483页

作者： Min Liu Jiafu Zhu Zhicheng Dong Innovative Center of molecular Genetics and Evolution Guangzhou Higher Education Mega CenterSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China Key Laboratory of South China Agricultural Plant molecular Analysis and Genetic Improvement South China Botanical Gardenthe Chinese Academy of SciencesGuangzhou 510650China

Plants have evolved efficient mechanisms for adapting to temperature fluctuations,known as heat stress response and heat stress *** the transcriptional regulatory network of plant heat stress response has been established,little is known about the genome-wide transcriptional changes occurring within the first several minutes after heat ***,we investigated the nascent RNA and mature messenger RNA(mRNA)from plant leaf tissues exposed to 5 min of heat shock treatment using global run-on sequencing and RNA sequencing *** a small group of genes were up-or downregulated at both the nascent RNA and mRNA *** plants that were already exposed to mild heat stress exhibited a more drastic alteration at multiple transcriptional steps than naive plants that had not experienced heat *** heat shock,we also observed the following:(i)engaged RNA polymerase Ⅱ accumulated downstream of transcription start sites;(ii)5′pausing release was a ratelimiting step for the induction of some heat shock protein genes;(iii)numerous genes switched transcription modes;(iv)pervasive read-through was induced at terminators;and(v)heat stress memory occurs at multiple steps of the transcription cycle,such as at Pol Ⅱ recruitment,5′pausing,elongation,and termination.

关键词： Transcription Nascent RNA biogenesis Arabidopsis Heat Shock Stress Memory

Epigenetic Repression of SATB1 by Polycomb Group Protein EZH2 in Epithelial Cells

在线全文

学校读者我要写书评

暂无评论

Chinese Medical sciences Journal 2010年第4期25卷 199-205页

作者： Chih-chuan Liang National Laboratory of Medical molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College

Objective To study the regulatory mechanism of SATB1 repression in cells other than T cells or erythroid cells, which have high expression level of SATB1. Methods HeLa epithelial cells were treated with either histone deacetylase inhibitor (HDACi) trichostatin A (TSA) or DNA methylation inhibitor 5-Aza-C before detecting SATB1 expression. Luciferase reporter system was applied to measure effects of EZH2 on SATB1 promoter activity. Over-expression or knockdown of EZH2 and subsequent quantitative reverse transcription-polymerase chain reaction were performed to determine the effect of this Polycomb group protein on SATB1 transcription. Chromatin immunoprecipitation (ChIP) assay was applied to measure enrichment of EZH2 and trimethylated H3K27 (H3K27me3) at SATB1 promoter in HeLa cells. K562 cells and Jurkat cells, both having high-level expression of SATB1, were used in the ChIP experiment as controls. Results Both TSA and 5-Aza-C increased SATB1 expression in HeLa cells. Over-expression of EZH2 reduced promoter activity as well as the mRNA level of SATB1, while knockdown of EZH2 apparently enhanced SATB1 expression in HeLa cells but not in K562 cells and Jurkat cells. ChIP assay results suggested that epigenetic silencing of SATB1 by EZH2 in HeLa cells was mediated by trimethylation modification of H3K27. In contrast, enrichment of EZH2 and H3K27me3 was not detected within proximal promoter region of SATB1 in either K562 or Jurkat cells. Conclusion SATB1 is a bona fide EZH2 target gene in HeLa cells and the repression of SATB1 by EZH2 may be mediated by trimethylation modification on H3K27.

关键词： SATB1 基因敲除上皮细胞组蛋白 Jurkat细胞镇压 HeLa细胞 DNA甲基化