Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H 1N 1 virus from peripheral blood memory B lymphocytes
Generation of human neutralizing monoclonal antibodies against the 2009 pandemic H 1N 1 virus from peripheral blood memory B lymphocytes作者机构：Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking UnionMedical College State Key Laboratory of Medical Molecular Biology Beijing China
出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)
年 卷 期：2013年第10卷第5期
页 面：403-412页
核心收录：
学科分类：0710[理学-生物学] 090602[农学-预防兽医学] 07[理学] 08[工学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：funded by National Natural Science Foundation of China US Civilian Research & Development Foundation
主 题：Epstein-Barr virus hemagglutinin memory B cells neutralizing monoclonal antibody 2009 pandemicinfluenza HIN1 virus
摘 要：The 2009 H 1N 1 influenza pandemic demonstrated the significance of a global health threat to human beings. Although pandemic H 1N 1 vaccines have been rapidly developed, passive serotherapy may offer superior immediate protection against infections in children, the elderly and immune-compromised patients during an influenza pandemic. Here, we applied a novel strategy based on Epstein-Barr virus （EBV）-immortalized peripheral blood memory B cells to screen high viral neutralizing monoclonal antibodies （MAbs） from individuals vaccinated with the 2009 pandemic H1N1 vaccine PANFLU.1. Through a massive screen of 13 090 immortalized memory B-cell clones from three selected vaccinees, seven MAbs were identified with both high viral neutralizing capacities and hemagglutination inhibition （HAl） activities against the 2009 pandemic H 1N 1 viruses. These MAbs may have important clinical implications for passive serotherapy treatments of infected patients with severe respiratory syndrome, especially children, the elderly and immunodeficient individuals. Our successful strategy for generating high-affinity MAbs from EBV-immortalized peripheral blood memory B ceils may also be applicable to other infectious or autoimmune diseases.
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