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Multimodality imaging assessments of response to metformin therapy for breast cancer in nude mice

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Chinese Medical Journal 2013年第19期126卷 3717-3722页

作者： MAO Yi XIA Rui WANG Lei WANG Yu-qing GAO Fa-bao molecular imaging Center Department of Radiology West ChinaHospital Sichuan University Chengdu Sichuan 610041 China

Background Metformin is the most widely used anti-diabetic drug in the *** increasing body of evidence shows metformin also blocks cell cycle progression and selectively induces apoptosis via caspase activation in some breast tumor ***-weighted imaging （DWl） and bioluminescence imaging （BLI） have great potential in the evaluation of the early response to cancer *** used DWl and BLI in evaluating the response of breast cancer to *** The luciferase-engineered human breast cancer cell line MDA-MB-231 was inoculated into the mammary fat pad of nude *** female nude mice bearing tumors were divided into two *** mice in the treatment group received metformin （2 mg/ml in drinking water daily） after tumor inoculation,and the mice in the control group were offered drinking water without any drug *** performed 7T magnetic resonance imaging and optical imaging every *** included T1-and T2-weighted imaging,DWl,and *** *** tumors were collected and subjected to histological *** The mean photons/second of tumors in the treatment group was （3.00±0.43）×106 at day one,（1.01±0.14）×107 at 2 weeks,（5.79±1.42）×107 at 4 weeks,and （2.33±0.70）×107 at 8 *** mean photons/second of tumors in the control group was （3.29±0.59）×106 at day one,（3.59±0.63）×107 at 2 weeks,（3.87±0.56）×108 at 4 weeks,and （4.12±1.72）x108 at 8 *** to the control group,the treatment group showed an obvious decrease in the mean bioluminescence （photons/s） of the tumors and fewer *** examination confirmed the presence of fewer *** showed the apparent diffusion coefficient （ADC） value of the tumors; the mean ADC value was （0.9287±0.04346）x10-3 mm2/s in the treated tumors and （0.7553±0.01804）x103 mm2/s in the untreated *** ADC value of tumors in the treatment group was significantly higher than the control tumors （P=0.0013）.Conclusions The growth and metastasis of MDA-MB-231 b

关键词： breast neoplasms metformin bioluminescent imaging magnetic resonance imaging

Advances in single-particle detection for DNA sensing

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Science China Chemistry 2017年第10期60卷 1285-1292页

作者： Fei Ma Ming Ren Chun-yang Zhang College of Chemistry Chemical Engineering and Materials Science Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of ShandongKey Laboratory of Molecular and Nano Probes Ministry of Education Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals Shandong Normal University Jinan 250014 China

Rapid, accurate and sensitive detection of particular DNA sequence is critical in fundamental biomedical research and clinical diagnostics. However, conventional approaches for DNA assay often suffer from cumbersome procedures, long analysis time and insufficient sensitivity. Recently, single-particle detection technology has emerged as a powerful tool in the biosensing area due to its significant advantages of ultrahigh sensitivity, low sample-consumption and rapid analysis time. Especially, the introduction of novel nanomaterials has greatly promoted the development of single-particle detection and its applications for DNA sensing. In this review, we summarize the recent advance in single-particle detection strategies for DNA sensing, and focus mainly on metallic nanoparticle-and semiconductor quantum dot-based single-particle detection. We highlight the emerging trends in this field as well.

关键词： single-particle detection DNA assay quantum dots metallic nanoparticles fluorescence resonance energy transfer

Highly Sensitive MoS_2–Indocyanine Green Hybrid for Photoacoustic imaging of Orthotopic Brain Glioma at Deep Site

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Nano-Micro Letters 2018年第3期10卷 115-126页

作者： Chengbo Liu Jingqin Chen Ying Zhu Xiaojing Gong Rongqin Zheng Ningbo Chen Dong Chen Huixiang Yan Peng Zhang Hairong Zheng Zonghai Sheng Liang Song Research Laboratory for Biomedical Optics and molecular imaging Shenzhen Key Laboratory for Molecular ImagingInstitute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen College of Advanced Technology University of Chinese Academy of Sciences Department of Medical Ultrasound The Third Affiliated Hospital of Sun Yat-Sen University Translational Medicine R&D Center Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Paul C.Lauterbur Research Center for Biomedical imaging Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences

Photoacoustic technology in combination with molecular imaging is a highly effective method for accurately diagnosing brain glioma. For glioma detection at a deeper site, contrast agents with higher photoacoustic imaging sensitivity are needed. Herein, we report a MoS_2–ICG hybrid with indocyanine green(ICG) conjugated to the surface of MoS_2 nanosheets. The hybrid significantly enhanced photoacoustic imaging sensitivity compared to MoS_2 nanosheets. This conjugation results in remarkably high optical absorbance across a broad near-infrared spectrum, redshifting of the ICG absorption peak and photothermal/photoacoustic conversion efficiency enhancement of ICG. A tumor mass of 3.5 mm beneath the mouse scalp was clearly visualized by using MoS_2–ICG as a contrast agent for the in vivo photoacoustic imaging of orthotopic glioma, which is nearly twofold deeper than the tumors imaged in our previous report using MoS_2 nanosheet. Thus, combined with its good stability and high biocompatibility, the MoS_2–ICG hybrid developed in this study has a great potential for high-efficiency tumor molecular imaging in translational medicine.

关键词： MoS2–ICG hybrid Orthotopic brain glioma Photoacoustic imaging molecular imaging

Advances in luminescent lanthanide complexes and applications

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Science China(Technological Sciences) 2018年第9期61卷 1265-1285页

作者： WEI Chen MA Li WEI HuiBo LIU ZhiWei BIAN ZuQiang HUANG ChunHui Beijing National Laboratory for molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and Applications College of Chemistry and Molecular Engineering Peking University

Luminescent lanthanide complexes have been widely investigated as light emitting materials in bio-imaging and sensing, solid state lighting and display, anti-fake tags and light conversion films, due to their characterized photophysical properties including large Stokes shift, long lifetime, and sharp emission spectrum, arising from the sensitized f-f transitions. In this review, we summarize the most recent advances in luminescent lanthanide complexes and their applications from 2015 to August 2017 concerning of general concepts to potential applications. We first introduce the basic concept of sensitized luminescence of lanthanide complexes and the strategies used for highly luminescent complexes. Then recent varieties of luminescent lanthanide complexes and their hybrid materials are presented. Finally, applications are discussed in detail.

关键词： luminescent lanthanide complex hybrid material bio-imaging sensing solid state lighting light conversion for solarcells

Nano-Medicine for Thrombosis:A Precise Diagnosis and Treatment Strategy

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Nano-Micro Letters 2020年第8期12卷 25-45页

作者： Min Su Qixuan Dai Chuan Chen Yun Zeng Chengchao Chu Gang Liu State Key Laboratory of molecular Vaccinology and molecular Diagnostics Center for Molecular Imaging and Translational Medicine School of Public HealthXiamen UniversityXiamen 361102People’s Republic of China State Key Laboratory of Physical Chemistry of Solid Surfaces The MOE Key Laboratory of Spectrochemical Analysis and InstrumentationCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005People’s Republic of China Department of Pharmacy Xiamen Medical CollegeXiamen 361023People’s Republic of China Xiamen Cardiovascular Hospital Xiamen UniversityXiamen 361102People’s Republic of China

Thrombosis is a global health issue and one of the leading factors of ***,its diagnosis has been limited to the late stages,and its therapeutic window is too narrow to provide reasonable and effective *** addition,clinical thrombolytics suffer from a short half-life,allergic reactions,inactivation,and unwanted tissue ***-medicines have gained extensive attention in diagnosis,drug delivery,and photo/sound/magnetic-theranostics due to their convertible ***,diagnosis and treatment of thrombosis using nano-medicines have also been widely *** review summarizes the recent advances in this area,which revealed six types of nanoparticle approaches:(1)in vitro diagnostic kits using“synthetic biomarkers”;(2)in vivo imaging using nano-contrast agents;(3)targeted drug delivery systems using artificial nanoparticles;(4)microenvironment responsive drug delivery systems;(5)drug delivery systems using biological nanostructures;and(6)treatments with external *** investigations of nano-medicines are believed to be of great significance,and some of the advanced drug delivery systems show potential applications in clinical theranotics.

关键词： Thrombosis Nano-medicine Diagnosis Thrombolytic therapy

Actin nucleator formins regulate the tension-buffering function of caveolin-1

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Journal of molecular Cell Biology 2021年第12期13卷 876-888页

作者： Xuemeng Shi Daijiao Tang Yifan Xing Shuangshuang Zhao Changyuan Fan Jin Zhong Yanqin Cui Kun Shi Yaming Jiu The Joint program in Infection and Immunity Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangzhou 510623China The Joint program in Infection and Immunity Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China Unit of Cell Biology and imaging Study of Pathogen Host Interaction The Center for MicrobesDevelopment and HealthCAS Key Laboratory of Molecular Virology and ImmunologyInstitut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China Unit of Viral Hepatitis CAS Key Laboratory of Molecular VirologyInstitut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China

Both the mechanosensitive actin cytoskeleton and caveolae contribute to active processes such as cell migration,morphogenesis,and vesicular *** distinct actin components are well studied,how they contribute to cytoplasmic caveolae,especially in the context of mechano-stress,has remained ***,we identify two actin-associated mobility stereotypes of caveolin-1(CAV-1)-marked intracellular vesicles,which are characterized as‘dwelling’and‘go and dwelling’.In order to exploit the reason for their distinct dynamics,elongated actin-associated formin functions are *** find drastically decreased density,increased clustering,and compromised motility of cytoplasmic CAV-1 vesicles resulting from lacking actin nucleator formins by both chemical treatment and RNA silencing of formin ***,hypo-osmosis-stimulated diminishing of CAV-1 is dramatically intensified upon blocking *** clustering of CAV-1 vesicles when cells are cultured on soft substrate is also aggravated under formin inhibition ***,we reveal that actin-associated formins are essential for maintaining the dynamic organization of cytoplasmic CAV-1 and importantly its sensitivity upon mechanical *** conclude that tension-controlled actin formins act as a safety valve dampening excessive tension on CAV-1 and safeguarding CAV-1 against mechanical damage.

关键词： caveolin-1 elongated actin filaments formins hypo-osmotic shock matrix stiffness

LiF@SiO2 nanocapsules for controlled lithium release and osteoarthritis treatment

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Nano Research 2018年第10期11卷 5751-5760页

作者： Trever Todd Zhenhui Lu Jinmin Zhao Benjamin Cline Weizhong Zhang Hongmin Chen Anil Kumar Wen Jiang Franklin West Samuel Franklin Li Zheng Jin Xie Department of Chemistry University of Georgia Athens Georgia 30602 USA Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration Guangxi Collaborative Innovation Center for Biomedicine Guangxi Key Laboratory of Regenerative Medicine The First Affiliated Hospital of Guangxi Medical University Nanning 530021 China Center for molecular imaging and Translational Medicine State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public Health Xiamen University Xiamen 361102 China Animal Dairy Science University of Georgia Athens Georgia 30602 USA Department of Small Animal Medicine and Surgery College of Veterinary Medicine University of Georgia Athens Georgia 30602 USA

Electrolytes can be taken orally or intravenously as supplements or thera- peutics. However, their therapeutic window may exceed the serum toxicity threshold, making systemic delivery a poor option. Local injection is also not adequate due to rapid diffusion of electrolytes. Here, we solved this issue with a nanocapsule technolog~ comprising an electrolyte nanocrystal as the drug filling and a silica sheath to regulate drug release rates. In particular, we prepared LiF@SiO2 nanocapsules and investigated their potential as a delivery system for lithium, which was shown in recent studies to be an effective therapeutic agent for osteoarthritis （OA）. We demonstrated that LiF@SiO2 can extend lithium release time from minutes to more than 60 h. After intra- articular （i.a.） injection into a rat OA model, the nanocapsules reduced the Osteoarthritis Research Society International （OARSI） score by 71% in 8 weeks while inducing no systemic toxicity. Our study opens new doors for improved delivery of electrolyte therapeutics, which have rarely been studied in the past.

关键词： osteoarthrifis controlled release hyaluronic acid lithium anti-inflammation

Superresolution live-cell imaging reveals that the localization of TMEM106B to filopodia in oligodendrocytes is compromised by the hypomyelination-related D252N mutation

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Science China(Life Sciences) 2023年第8期66卷 1858-1868页

作者： Shijia Xing Xiaolu Zheng Huifang Yan Yanquan Mo Ruoyu Duan Zhixing Chen Kunhao Wang Kai Gao Tongsheng Chen Shiqun Zhao Jingmin Wang Liangyi Chen National Biomedical imaging Center State Key Laboratory of Membrane BiologyBeijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China Department of Pediatrics Peking University First HospitalBeijing 100034China PKU-IDG/McGovern Institute for Brain Research Beijing 100871China National Biomedical imaging Center Institute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China Key Laboratory of Laser Life Science Ministry of EducationCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China National Center for Children’s Health Department of NeurologyBeijing Children’s HospitalCapital Medical UniversityBeijing 100045China

Hypomyelination leukodystrophies constitute a group of heritable white matter disorders exhibiting defective myelin *** identified as a lysosomal protein,the TMEM106B D252N mutant has recently been associated with ***,how lysosomal TMEM106B facilitates myelination and how the D252N mutation disrupts that process are poorly *** used superresolution Hessian structured illumination microscopy(Hessian-SIM)and spinning discconfocal structured illumination microscopy(SD-SIM)to find that the wild-type TMEM106B protein is targeted to the plasma membrane,filopodia,and lysosomes in human *** D252N mutation reduces the size of lysosomes in oligodendrocytes and compromises lysosome changes upon starvation *** importantly,we detected reductions in the length and number of filopodia in cells expressing the D252N ***1 is the most abundant myelin protein that almost entirely colocalizes with TMEM106B,and coexpressing PLP1 with the D252N mutant readily rescues the lysosome and filopodia phenotypes of ***,interactions between TMEM106B and PLP1 on the plasma membrane are essential for filopodia formation and myelination in oligodendrocytes,which may be sustained by the delivery of these proteins from lysosomes via exocytosis.

关键词： superresolution imaging oligodendrocyte TMEM106B lysosome filopodia

The First-in-Human Whole-Body Dynamic Pharmacokinetics Study of Aptamer

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Research 2024年第1期2023卷 119-130页

作者： Ding Ding Haitao Zhao Dali Wei Qinglai Yang Cai Yang Ruowen Wang Yumei Chen Lianghua Li Shuxian An Qian Xia Gang Huang Jianjun Liu Zeyu Xiao Weihong Tan Institute of molecular Medicine(IMM) Renji HospitalState Key Laboratory of Oncogenes and Related GenesShanghai Jiao Tong University School of Medicineand College of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Nuclear Medicine Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Center for molecular imaging Probes Cancer Research InstituteUniversity of South ChinaHengyangHunan 421001China The Key Laboratory of Zhejiang Province for Aptamers and Theranostics Zhejiang Cancer HospitalHangzhou Institute of Medicine(HIM)Chinese Academy of SciencesHangzhouZhejiang 310022China molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangshaHunan 410082China Shanghai Key Laboratory of molecular imaging Shanghai University of Medicine and Health SciencesShanghai 201318China Department of Pharmacology and Chemical Biology Shanghai Jiao Tong University School of MedicineShanghai200127China

Serving as targeting ligands,aptamers have shown promise in precision ***,the lack of knowledge of the biosafety and metabolism patterns in the human body largely impeded aptamers’clinical *** bridge this gap,here we report the first-in-human pharmacokinetics study of protein tyrosine kinase 7 targeted SGC8 aptamer via in vivo PET tracking of gallium-68(^(68)Ga)radiolabeled *** specificity and binding affinity of a radiolabeled aptamer,named ^(68)Ga[Ga]-NOTA-SGC8,were maintained as proven in *** preclinical biosafety and biodistribution evaluation confirmed that aptamers have no biotoxicity,potential mutation risks,or genotoxicity at high dosage(40 mg/kg).Based on this result,a first-in-human clinical trial was approved and carried out to evaluate the circulation and metabolism profiles,as well as biosafety,of the radiolabeled SGC8 aptamer in the human *** advantage of the cutting-edge total-body PET,the aptamers’distribution pattern in the human body was acquired in a dynamic fashion.

关键词： metabolism dosage Whole