检索结果-南通市图书馆

Glucocorticoid receptor–IRS-1 axis controls EMT and the metastasis of breast cancers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of molecular cell biology 2019年第12期11卷 1042-1055页

作者： Weiwei Shi Dongmei Wang Xinwang Yuan Yi Liu Xiaojie Guo Jingsong Li Jianguo Song State Key Laboratory of cell biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China

Glucocorticoid receptor (GR) is involved in the transcriptional regulation of genes that are important for various biological functions, including tumor growth and metastatic progression. However, the cellular and biological effects of GR remain poorly understood. Here, we investigated the role of GR and its underlying mechanism in mediating breast cancer cell survival and metastasis. We observed that the GR levels were increased in drug-resistant breast cancer cells and in metastatic breast cancer samples. GR promoted tumor cell invasion and lung metastasis in vivo. The GR expression levels were negatively correlated with the survival rates of breast cancer patients. Both ectopic expression and knockdown of GR revealed that GR is a strong inducer of epithelial-to-mesenchymal transition (EMT), which is consistent with its effects on cell survival and metastasis. GR suppressed the expression of insulin receptor substrate 1 (IRS-1) by acting as an IRS-1 transcriptional repressor. In addition, GR has an opposite effect on the expression levels of IRS-2, indicating that GR is able to differentially regulate the IRS-1 and IRS-2 expression. The cellular and biological effects elicited by GR were consistent with the reduced levels of IRS-1 observed in cancer cells, and GR-mediated IRS-1 suppression activated the ERK2 MAP kinase pathway, which is required for GR-mediated EMT. Taken together, our results indicate that GR–IRS-1 signaling axis plays an essential role in regulating the survival, invasion, and metastasis of breast cancer cells.

关键词： metastasis breast invasion

Remodeling of astrocyte secretome in amyotrophic lateral sclerosis:uncovering novel targets to combat astrocyte-mediated toxicity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Translational Neurodegeneration 2022年第1期11卷 84-97页

作者： Winanto Ng Shi-Yan Ng Institute of molecular and cell biology A*STAR Research EntitiesSingapore 138673Singapore

Amyotrophic lateral sclerosis(ALS)is an adult-onset paralytic disease characterized by progressive degeneration of upper and lower motor neurons in the motor cortex,brainstem and spinal *** neuron degeneration is typically caused by a combination of intrinsic neuronal(cell autonomous)defects as well as extrinsic(non-cell autonomous)factors such as astrocyte-mediated *** are highly plastic cells that react to their microenvironment to mediate relevant *** neurodegeneration,astrocytes often turn reactive and in turn secrete a slew of factors to exert pro-inflammatory and neurotoxic *** efforts have been carried out to characterize the diseased astrocyte secretome over the years,revealing that pro-inflammatory chemokines,cytokines and microRNAs are the main players in mediating neuronal *** metabolomic technologies mature,these studies begin to shed light on neurotoxic metabolites such as secreted *** this focused review,we will discuss changes in the astrocyte secretome during *** particular,we will discuss the components of the reactive astrocyte secretome that contribute to neuronal death in ALS.

关键词： Motor neuron disease Astrocyte Astrocyte-mediated toxicity Secretome

MAPK signaling in inflammation-associated cancer development

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & cell 2010年第3期1卷 218-226页

作者： Pengyu Huang Jiahuai Han Lijian Hui Laboratory of molecular cell biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Key Laboratory of the Ministry of Education for cell biology and Tumor cell Engineering School of Life SciencesXiamen UniversityXiamen 361005China

Mitogen-activated protein(MAP)kinases comprise a family of protein-serine/threonine kinases,which are highly conserved in protein structures from unicellular eukaryotic organisms to multicellular organisms,including *** kinases,including ERKs,JNKs and p38s,are regulated by a phosphorelay cascade,with a prototype of three protein kinases that sequentially phosphorylate one *** transduce extracellular signals into a variety of cellular processes,such as cell proliferation,survival,death,and *** with their essential cellular functions,MAPKs have been shown to play critical roles in embryonic development,adult tissue homeostasis and various *** this review,we discuss recent findings that reveal the profound impact of these pathways on chronic inflammation and,particularly,inflammation-associated cancer development.

关键词： inflammation cancer homeostasis

cDNA cloning, functional expression and cellular localization of rat liver mitochondrial electron-transfer flavoprotein-ubiquinone oxidoreductase protein

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2005年第4期48卷 357-367页

作者： HUANG Shengbing SONG Wei LIN Qishui Key Laboratory of molecular cell biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China

A membrane-bound protein was purified from rat liver *** being di-gested with V8 protease,two peptides containing identical 14 amino acid residue sequences were *** the 14 amino acid peptide derived DNA sequence as gene specific primer,the cDNA of correspondent gene 5′-terminal and 3′-terminal were obtained by RACE *** full-length cDNA that encoded a protein of 616 amino acids was thus cloned,which included the above mentioned peptide *** full length cDNA was highly homologous to that of human ETF-QO,indicating that it may be the cDNA of rat ***-QO is an iron sulfur protein located in mitochondria inner membrane containing two kinds of redox center:FAD and[4Fe-4S]*** comparing the sequence from the cDNA of the 616 amino acids protein with that of the mature protein of rat liver mitochondria,it was found that the N terminal 32 amino acid residues did not exist in the mature protein,indicating that the cDNA was that of *** the cDNA was expressed in Saccharomyces cerevisiae with inducible vectors,the protein product was enriched in mitochondrial fraction and exhibited electron transfer activity(NBT re-ductase activity)of *** demonstrated that the 32 amino acid peptide was a mito-chondrial targeting peptide,and both FAD and iron-sulfur cluster were inserted properly into the expressed ***-QO had a high level expression in rat heart,liver and *** fu-sion protein of GFP-ETF-QO co-localized with mitochondria in COS-7 cells.

关键词： electron transfer flavoprotein ubiquinone oxidoreductase precursor rat protein purification gene clone tissuespecificity.

CaSfl1 plays a dual role in transcriptional regulation in Candida albicans

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2008年第17期53卷 2624-2631页

作者： ZHANG TingTing LI Di LI WanJie WANG Yue SANG JianLi Key Laboratory of cell Proliferation and Regulation of Ministry of Education Beijing Normal University Beijing 100875 China Candida albicans molecular and cell biology Laboratory Institute of Molecular and Cell Biology Proteos 61 Biopolis Drive Singapore 138673 Singapore

As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene indeed affected cell flocculation and filamentation. In addition, by RT-PCR we found that while Casfl1 repressed the expression of several hypha-specific genes including HWP1, ECE1, ALS1, ALS3, and FLO8, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfl1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.

关键词：假丝酵母 CaSFL1 基因注入形态发生转录规则

Small Ubiquitin-Like Modifier Conjugating Enzyme with Active Site Mutation Acts as Dominant Negative Inhibitor of SUMO Conjugation in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant biology 2013年第1期55卷 75-82页

作者： Konstantin Tomanov Christian Hardtke Ruchika Budhiraja Rebecca Hermkes George Coupland Andreas Bachmair Department of Biochemistry and cell biology Max F. Perutz Laboratories Center for Molecular Biology University of Vienna Department of Plant molecular biology University of Lausanne Department of Plant Developmental biology Max Planck Institute for Plant Breeding Research

Small ubiquitin-like modifier （SUMO） conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.

关键词： Agroinfection conjugating enzyme dominant-negative active site mutation small ubiquitin-like modifier conjugation small ubiquitin-like modifier.

Sinoatrial node pacemaker cells: cardiomyocyte- or neuron-like cells?

在线全文

学校读者我要写书评

暂无评论

Protein & cell 2021年第7期12卷 518-519页

作者： Bin Zhou State Key Laboratory of cell biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China

The sinoatrial node(SAN)is the headquarter of heartbeat throughout our lifetime(Lakatta et al.,2010;Cingolani et al.t 2018;Peters et al.,2020).Every beat of the heart is triggered by a bioelectric pulse spontaneously released by SAN pacemaker cells(SANPCs)(Yaniv et al.,2014;Yavari et al.,2017).In adult human heart,the SAN is a crescent-shaped structure of 1-2 cm long and 0.5 cm wide,which is located at the junction of the superior vena cava and the right atrium and lies along the sulcus terminalis(John et al.,2016).However,the nature of SANPCs remains incompletely *** general,SANPCs have long been considered as specialized cardiomyocytes(Van Eif et al.,2018;Linscheid et al.,2019;Galang et al.,2020;).

关键词： incomplete specialized lifetime

A pancreatic player in dementia:pathological role for islet amyloid polypeptide accumulation in the brain

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第10期18卷 2141-2146页

作者： Angelina S.Bortoletto Ronald J.Parchem Center for cell and Gene Therapy Stem Cell and Regenerative Medicine CenterDepartment of NeuroscienceDepartment of Molecular and Cellular BiologyTranslational Biology and Molecular Medicine ProgramMedical Scientist Training ProgramBaylor College of MedicineHoustonTXUSA

Type 2 diabetes mellitus patients have a markedly higher risk of developing *** multiple factors contribute to this predisposition,one of these involves the increased secretion of amylin,or islet amyloid polypeptide,that accompanies the pathophysiology of type 2 diabetes *** amyloid polypeptide accumulation has undoubtedly been implicated in various forms of dementia,including Alzheimer’s disease and vascular dementia,but the exact mechanisms underlying islet amyloid polypeptide’s causative role in dementia are *** this review,we have summarized the literature supporting the various mechanisms by which islet amyloid polypeptide accumulation may cause neuronal damage,ultimately leading to the clinical symptoms of *** discuss the evidence for islet amyloid polypeptide deposition in the brain,islet amyloid polypeptide interaction with other amyloids implicated in neurodegeneration,neuroinflammation caused by islet amyloid polypeptide deposition,vascular damage induced by islet amyloid polypeptide accumulation,and islet amyloid polypeptide-induced *** are very few therapies approved for the treatment of dementia,and of these,clinical responses have been controversial at ***,investigating new,targetable pathways is vital for identifying novel therapeutic strategies for treating *** such,we conclude this review by discussing islet amyloid polypeptide accumulation as a potential therapeutic target not only in treating type 2 diabetes mellitus but as a future target in treating or even preventing dementia associated with type 2 diabetes mellitus.

关键词： Alzheimer’s disease amylin amyloid dementia diabetes human islet amyloid polypeptide islet amyloid polypeptide protofibrils type 2 diabetes mellitus vascular dementia

Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2021年第6期23卷 590-599页

作者： Ying-Hua Wang Meng Yan Xi Zhang Xin-Yu Liu Yi-Fu Ding Chong-Ping Lai Ming-Han Tong Jin-Song Li State Key Laboratory of cell biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China State Key Laboratory of molecular biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells(SSCs).However,such therapy for infertility has not been experimentally investigated *** this study,a mouse model with an X-linked testis-expressed 11(TEX11)mutation(Tex11PM/Y)identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome ***11PM/Y SSCs could be isolated and expanded in vitro normally,and the mutation was corrected by clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated endonuclease 9(Cas9),leading to the generation of repaired SSC ***-genome sequencing demonstrated that the mutation rate in repaired SSCs is comparable with that of autonomous mutation in untreated Tex11PM/Y SSCs,and no predicted off-target sites are *** SSCs could restore spermatogenesis in infertile males and give rise to fertile offspring at a high *** summary,our study establishes a paradigm for the treatment of male azoospermia by combining in vitro expansion of SSCs and gene therapy.

关键词： azoospermia gene therapy male infertility meiotic arrest spermatogonial stem cells testis-expressed 11