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SHANK2 is a frequently amplified oncogene with evolutionarily conserved roles in regulating Hippo signaling

Protein & Cell 2021年第3期12卷 174-193页

作者： Liang Xu Peixue Li Xue Hao Yi Lu Mingxian Liu Wenqian Song Lin Shan Jiao Yu Hongyu Ding Shishuang Chen Ailing Yang Yi Arial Zeng Lei Zhang Hai Jiang State Key laboratory of Cell biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Bio-Research Innovation Center Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesSuzhou 215121China

Dysfunction of the Hippo pathway enables cells to evade contact inhibition and provides advantages for cancerous ***,for a significant portion of human cancer,how Hippo signaling is perturbed remains *** answer this question,we performed a genome-wide screening for genes that affect the Hippo pathway in Drosophila and cross-referenced the hit genes with human cancer *** our screen,Prosap was identified as a novel regulator of the Hippo pathway that potently affects tissue ***,a mammalian homolog of Prosap,SHANK2,is the most frequently amplified gene on 11 q13,a major tumor amplicon in human *** amplification profile in this 11q13 amplicon clearly indicates selective pressure for SHANK2 *** importantly,across the human cancer genome,SHANK2 is the most frequently amplified gene that is not located within the Myc *** studies in multiple human cell lines confirmed that SHANK2 overexpression causes deregulation of Hippo signaling through competitive binding for a LATS1 activator,and as a potential oncogene,SHANK2 promotes cellular transformation and tumor formation in *** cancer cell lines with deregulated Hippo pathway,depletion of SHANK2 restores Hippo signaling and ceases cellular *** together,these results suggest that SHANK2 is an evolutionarily conserved Hippo pathway regulator,commonly amplified in human cancer and potently promotes *** study for the first time illustrated oncogenic function of SHANK2,one of the most frequently amplified gene in human ***,given that in normal adult tissues,SHANK2 s expression is largely restricted to the nervous system,SHANK2 may represent an interesting target for anticancer therapy.

关键词： SHANK2 oncogene Hippo signaling cancer

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Predictive value of postprocedural leukocyte count on myocardial perfusion, left ventricular function and clinical outcomes in ST-elevated myocardial infarction after percutaneous coronary intervention

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Chinese Medical Journal 2012年第6期125卷 1023-1029页

作者： HE Rong LI Hai-yan GUO Li-jun ZHANG Fu-chun NIU Jie ZHANG Yong-zhen WANG Gui-song YANG Zhen-hua GAO Wei Department of Cardiology Peking University Third HospitalKey Laboratory of Cardiovascular Molecular Biology and Regulatory PeptidesMinistry of HealthKey Laboratory of Molecular Cardiovascular SciencesMinistry of EducationBeijing 100191China

Background Baseline white blood cell （WBC） count was correlated with ischemic events occurrence in patients with ST-elevated myocardial infarction （STEMI）. However, circulating WBC count is altered after percutaneous coronary intervention （PCI）. The aim of this study was to assess the relationship between postprocedural WBC count and clinical outcomes in STEMI patients who underwent PCI. Methods A total of 242 consecutive acute STEMI patients who underwent successful primary PCI were enrolled and followed up for two years. WBC counts were measured within 12 hours after PCI. ST-segment resolution （ST-R） and myocardial blush grades （MBG） were evaluated immediately after PCI. Left ventricular ejection fraction （LVEF） was obtained at baseline and 12-18 months after PCI. Results Postprocedural WBC count was an independent inverse predictor of ST-R （OR 0.80, P 〈0.0001） and MBG 3 （OR 0.82, P 〈0.0001）. It was negatively correlated with LVEF （baseline r=0.22, P=0.001; 12-18 months r=0.29, P 〈0.0001）. The best cutoff value of WBC for predicting death was determined to be 13.0×10^9/L. The patients with a postprocedural WBC count above 13.0×10^9/L showed a significantly lower cumulative survival rate （30 days, 82.4% vs. 99.0%, P 〈0.0001 and 2 years 75.0% vs. 96.4%, P 〈0.0001）. Multivariate Cox regression analysis showed that a postprocedural WBC count was a strong independent predictor of 30-day mortality （HR 8.48, P=0.019） and 2-year mortality （HR 4.93, P=0.009）. Conclusions Increased postprocedural WBC count is correlated with myocardial malperfusion and left ventricular dysfunction, and is an independent predictor of poor clinical outcomes in STEMI patients who underwent PCI.

关键词： angioplasty, transluminal, percutaneous coronary leukocyte myocardial infarction left ventricular dysfunction prognosis

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Beyond Antibiotics:Photo/Sonodynamic Approaches for Bacterial Theranostics

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Nano-Micro Letters 2020年第10期12卷 313-335页

作者： Xin Pang Dengfeng Li Jing Zhu Jingliang Cheng Gang Liu Henan Key laboratory of Functional Magnetic Resonance Imaging and molecular Imaging Department of Magnetic Resonance ImagingThe First Affiliated Hospital of Zhengzhou University450052 ZhengzhouPeople’s Republic of China State Key laboratory of molecular Vaccinology and molecular Diagnostics and Center for molecular Imaging and Translational Medicine School of Public HealthXiamen University361102 XiamenPeople’s Republic of China Amoy Hopeful Biotechnology Co. Ltd361027 XiamenPeople’s Republic of China

Rapid evolution and propagation of multidrug resistance among bacterial pathogens are outpacing the development of new antibiotics,but antimicrobial photodynamic therapy(aPDT)provides an excellent *** treatment depends on the interaction between light and photoactivated sensitizer to generate reactive oxygen species(ROS),which are highly cytotoxic to induce apoptosis in virtually all microorganisms without resistance *** replacing light with low-frequency ultrasonic wave to activate sensitizer,a novel ultrasounddriven treatment emerges as antimicrobial sonodynamic therapy(aSDT).Recent advances in aPDT and aSDT reveal golden opportunities for the management of multidrug resistant bacterial infections,especially in the theranostic application where imaging diagnosis can be accomplished facilely with the inherent optical characteristics of sensitizers,and the generated ROS by aPDT/SDT cause broad-spectrum oxidative damage for *** this review,we systemically outline the mechanisms,targets,and current progress of aPDT/SDT for bacterial theranostic ***,potential limitations and future perspectives are also highlighted.

关键词： Photodynamic therapy Sonodynamic therapy Theranostics Multidrug resistant Reactive oxygen species

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Science Letters:Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第6期7卷 497-502页

作者： LIAO Fei ZHAO Yun-sheng ZHAO Li-na TAO Jia ZHU Xiao-yun LIU Lan laboratory of Bioinformatics and molecular Engineering Chongqing University of Medical Sciences Chongqing 400016 China Department of Biochemistry and molecular biology Chongqing University of Medical Sciences Chongqing 400016 China Affiliated Children＇s Hospital Chongqing University of Medical Sciences Chongqing 400016 China

A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action （A0） was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance （Ab） was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from AA, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV〈4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine 〈0.32 mmol/L in serum had no significant effects. △A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.

关键词： Background absorbance Bland-Altman analysis Kinetic uricase method Reaction curve fitting Serum uric acid assay

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Functional Diversity of CYCLOIDEA-like TCP Genes in the Control of Zygomorphic Flower Development in Lotus japonicus

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Journal of Integrative Plant biology 2013年第3期55卷 221-231页

作者： Shilei Xu Yonghai Luo Zhigang Cai Xiangling Cao Xiaohe Hu Jun Yang Da Luo National Key laboratory of Plant molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciencesthe Chinese Academy of Sciences Graduate University of the Chinese Academy of Sciences laboratory of Cancer Cell biology Tianjin Medical University Cancer Hospital and Institute School of Life Sciences Sun Yat-sen University

CYCLOIDEA （CYC）-like TCP genes play key roles in dorsoventral differentiation of zygomorphic flowers in Papilionoideae legumes. In this study, we analyzed the kew mutants whose flowers lost lateral identity, and investigated the diverse functions of three LjCYC genes during zygomorphic flower development in the model legume Lotus japonicus. We showed that kew1 and kew3 are allelic mutants of LjCYC3, a CYC-like TCP gene. Through transgenic experiments, it was shown that LjCYC1 possesses dorsal activity similar to LjCYC2, and that LjCYC3 alone is sufficient to confer lateral activity, and an epistatic effect between dorsal and lateral activities was identified. Sequence analysis revealed a striking alteration at the 3 end of the LjCYC3 open reading frame （ORF） in comparison with those of LjCYC1 and LjCYC2 ORFs. Furthermore, it was found that LjCYC proteins could interact with each other and possess different activities by means of a transcriptional activity assay. Our data demonstrate that the sequence variation and the subsequent alteration of protein property play important roles in the functional diversity of different LjCYC genes in controlling zygomorphic flower development in Lotus japonicus.

关键词： Activity of transcriptional activation kew mutants Lotusjaponicus TCP genes zygomorphic flower development.

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Dendrimer nanocarriers drug action: perspective for neuronal pharmacology

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Neural Regeneration Research 2015年第7期10卷 1029-1031页

作者： Felipe Vidal Leonardo Guzman laboratory of molecular Neurobiology Department of PhysiologyFaculty of Biological Sciences University of Concepcion

General aspects：Currently,several strategies have been used to overcome common problems found in pharmacological therapies,such as insolubility,reduced bioavailability and low specificity of *** of the most promising alternatives proposed is the use of dendrimeric drug nanocarriers,polymeric chemical structures of diverse nature that contain,transport and deliver the desired drug in biological systems.

关键词： PAMAM perspective for neuronal pharmacology

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Genetic diversity of Ustilago hordei in Tibetan areas as revealed by RAPD and SSR

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Journal of Integrative Agriculture 2016年第10期15卷 2299-2308页

作者： ZHOU Yu CHAO Gui-mei LIU Jia-jia ZHU Ming-qi WANG Yang FENG Bai-li State Key laboratory of Crop Stress biology for Arid Areas/College of Agronomy Northwest A&F University State Key laboratory of Crop Stress biology for Arid Areas/College of Plant Protection Northwest A&F University

Covered smut, which is caused by Ustilago hordei（Pers.） Lagerh., is one of the most damaging diseases of highland barley（Hordeum vulgare Linn. var. nudum Hook. f） in Tibetan areas of China. To understand the molecular diversity of U. hordei, a total of 27 isolates, which were collected from highland barley plants from Tibet, Sichuan, Qinghai, and Gansu provinces/autonomous region, were analyzed using random amplified polymorphic DNA（RAPD） and simple sequence repeat（SSR） markers. Among the 100 RAPD primers used, 24 primers exhibited polymorphism. A total of 111 fragments were amplified, of which 103 were polymorphic with a polymorphic rate of 92.79%. The average observed number of alleles（Na）, effective number of alleles（Ne）, Nei＇s genetic diversity（H）, Shannon＇s information index（I） and polymorphism information content（PIC） value in the RAPD markers were 1.9279, 1.5016, 0.2974, 0.4503 and 0.6428, respectively. For the SSR markers, 40 of the 111 primer pairs exhibited polymorphism and provided a total of 119 bands, of which 109 were polymorphic and accounted for 91.60% of the total bands. The Na, Ne, H, I and PIC values of the SSR markers were 1.9160, 1.4639, 0.2757, 0.4211 and 0.4340, respectively. The similarity coefficients ranged from 0.4957 to 0.9261 with an average of 0.7028 among all the 27 isolates used. The dendrogram, which was developed based on the RAPD and SSR combined marker dataset showed that the 27 U. hordei isolates were divided into 3 clusters at similarity coefficient of 0.7314. We determined that RAPD and SSR markers can be successfully used to assess the genetic variation among U. hordei isolates. The RAPD markers revealed higher levels of genetic polymorphism than did the SSR markers in this study. There existed a moderate genetic difference among isolates. The molecular variation and differentiation was somewhat associated with geographical origin but not for all of the isolates.

关键词： highland barley Ustilago hordei RAPD SSR genetic diversity

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Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry

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Science China Chemistry 2014年第3期57卷 442-446,1-3页

作者： ZHANG YanDong LI HaiFang MA Yuan LIN JinMing Beijing Key laboratory of Microanalytical Methods and Instrumentation The Key laboratory of Bioorganic Phosphorus Chemistry & Chemical biology Department of Chemistry Tsinghua University

The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological *** this work,a mass spectrometry-based method for direct,in situ analysis of lipids in cells was *** cells were directly cultured on ITO-coated glass and then analyzed by matrix-assisted laser desorption ionization-mass spectrometry(MALDI-MS).The matrix application process was achieved by electrospray coating,which produced a homogenous layer of matrix crystal on the *** detection results and reproducibility are *** this method,a profile of abundant membrane lipids is generated,which is characteristic of cell *** conclusion,this in situ MALDI-MS cellular lipid analysis method provides a platform for sensitive and robust molecular profiling of mammalian cells.

关键词： lipid profiling MALDI-MS linear discriminative analysis

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High-Quality Single-Layer Graphene via Reparative Reduction of Graphene Oxide

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Nano Research 2011年第5期4卷 434-439页

作者： Boya Dai Lei Fu Lei Liao Nan Liu Kai Yan Yongsheng Chen Zhongfan Liu Center for Nanochemistry Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable Species College of Chemistry and Molecular Engineering Peking University Beijing 100871 China College of Chemistry Nankai University Tianjin 300071 China

Reduction of graphene oxide （GO） is a promising low-cost synthetic approach to bulk graphene, which offers an accessible route to transparent conducting films and flexible electronics. Unfortunately, the release of oxygen-containing functional groups inevitably leaves behind vacancies and topological defects on the reduced GO sheet, and its low electrical conductivity hinders the development of practical applications. Here, we present a strategy for real-time repair of the newborn vacancies with carbon radicals produced by thermal decomposition of a suitable precursor. The sheet conductivity of thus-obtained single-layer graphene was raised more than six-fold to 350-410 S/cm （whilst retaining 〉96% transparency）. X-ray photoelectron spectroscopy （XPS） and Raman spectroscopy revealed that the conductivity enhancement can be attributed to the formation of additional sp2-C structures. This method provides a simple and efficient process for obtaining highly conductive transparent graphene films.

关键词： Graphene graphene oxide reparative reduction transparent flexible electrode

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Cocrystal engineering:towards high-performance near-infrared organic phototransistors based on donor-acceptor charge transfer cocrystals

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Science China Chemistry 2023年第1期66卷 266-272页

作者： Fei Li Lei Zheng Yajing Sun Shuyu Li Lingjie Sun Fangxu Yang Weibing Dong Xiaotao Zhang Wenping Hu Tianjin Key laboratory of molecular Optoelectronic Sciences Institute of Molecular Aggregation Science of Tianjin UniversityTianjin 300072China Tianjin Key laboratory of molecular Optoelectronic Sciences School of ScienceTianjin UniversityTianjin 300072China Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityFuzhou 350207China Key laboratory of Resource Chemistry and Eco-environmental Protection in Qinghai-Tibet Plateau Qinghai Minzu UniversityXining 810007China

Near-infrared organic phototransistors have wide application prospects in many *** active materials with the high mobility and near-infrared response are critical to building high-performance near-infrared organic phototransistors,which are scarce at ***,a new charge transfer cocrystal using 5,7-dihydroindolo[2,3-b]carbazole(5,7-ICZ)as the donor and 2,2’-(benzo[1,2-b:4,5-b’]dithiophene-4,8-diylidene)dimalononitrile(DTTCNQ)as the acceptor is properly designed and prepared in a stoichiometric ratio(D:A=1:1),which not only displays a high electron mobility of 0.15 cm^(2)V^(-1)s^(-1) and very low dark current,but also can serve as the active layer materials in the region of near-infrared detection due to the narrowed band gap and good charge transport properties.A high photosensitivity of 1.8×10^(4),the ultrahigh photoresponsivity of 2,923 A W-1and the high detectivity of 4.26×10^(11)Jones of the organic near-infrared phototransistors are obtained.

关键词： organic semiconductors charge transporting properties organic cocrystals organic field effect transistors near-infrared organic phototransistors

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