Science Letters:Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method

作     者：LIAO Fei ZHAO Yun-sheng ZHAO Li-na TAO Jia ZHU Xiao-yun LIU Lan 

作者机构：Laboratory of Bioinformatics and Molecular Engineering Chongqing University of Medical Sciences Chongqing 400016 China Department of Biochemistry and Molecular Biology Chongqing University of Medical Sciences Chongqing 400016 China Affiliated Children＇s Hospital Chongqing University of Medical Sciences Chongqing 400016 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2006年第7卷第6期

页      面：497-502页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：Project (No. 30200266) supported by the National Natural Science Foundation of China 

主　　题：Background absorbance Bland-Altman analysis Kinetic uricase method Reaction curve fitting Serum uric acid assay 

摘      要：A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action （A0） was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance （Ab） was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from AA, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV〈4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine 〈0.32 mmol/L in serum had no significant effects. △A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.