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In vitro and in vivo evaluation of micro-alloyed magnesium for potential application in alveolar bone fixation screws

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Journal of Materials Science & Technology 2023年第13期144卷 62-69页

作者： Hongyan Tang Qing Li Min Li Xuenan Gu Chengkung Cheng Yubo Fan Key Laboratory of biomechanics and Mechanobiology(Beihang University) Ministry of EducationBeijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing 100083China Center of Digital Dentistry Peking University School and Hospital of StomatologyBeijing 100081China National Engineering research Center of Oral biomaterials and Digital Medical Devices Beijing 100081China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200030China

Alveolar bone augmentation with fixation screws has difficulties such as non-degradable materials that could lead to secondary surgery and insufficient osseointegration due to the subgingival environment in dental *** degradability and a high degree of osteogenesis,Mg alloy is a successful biodegrad-able material for orthopedic applications,and its application in dentistry has made certain ***-ever,considering the unique subgingival healing properties of oral implants,there is still a gap between the desired material properties for clinical applications and available ***,studies on the use of Mg-based fixation screws for dentistry applications are still *** this study,we reported a magnesium alloy with low combined addition of strontium and *** mechanical properties,degradation behavior,osteogenesis,and gingival compatibility were systematically investigated for assess-ing its potential application in alveolar bone fixation *** the alloying element content restricted to 0.3 wt.%,Mg-Sr-La alloy still exhibited good mechanical properties,with yield tensile and compressive strength twice higher than those of pure *** in vitro degradation rate of this alloy was 0.10 mm y-1,which was slightly slower than high-purity *** indirect and direct cell assay confirmed the elevated osteoblastic differentiation of MC3T3-E1 and migration of HGF-1 ***,Mg-Sr-La alloy demon-strated a relatively slow degradation in the maxillary bone of Beagles.A remarkable promotion of the bone-implant contacts and significantly decreased fibrous encapsulation was observed in the subgingival environment,implying superior osseointegration of the experimental alloy than the titanium *** empirical findings here reveal the great potential of Mg-Sr-La alloy for the application in alveolar bone fixation devices.

关键词： Magnesium alloys Alveolar ridge augmentation Fixation screws Osseointegration

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STRAIN AND STRAIN RATE:SIGNALS FOR BONE ADAPTATION.

STRAIN AND STRAIN RATE:SIGNALS FOR BONE ADAPTATION.

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The Third International Congress on Osteoporosis

作者： D.B.Burr Departmentsof Anatomy and Orthopedic Surgery Biomechanics and Biomaterials Research CenterIndiana University School of MedicineIndianapolisIN 46206 USA

＜正＞Rate-related phenomena are critical to the mechanically adaptive response of bone.O’Connor et al.[1]first showed the association between strain rate and new bone formation,but failed to show that increased rate caused the bone *** and Lanyon[2],using the rat ulnar bending model,showed that strain rate provides a greater osteogenic stimulus for bone formation than strain ***,this work did not consider the viscoelastic response of bone to loading,and was performed in immature rats loaded through an open growth ***,Turner[3,4]showed cause and effect between strain rate and bone adaptation with two *** the 4-point rat tibial bending model,he showed that increasing the frequency of loading while maintaining a constant strain magnitude caused a significant increase in bone formation rate at frequencies of 0.5 *** the duration of loading varied in this experiment,Turner et al[4]performed another experiment in which they altered strain rate,but kept frequency,duration and peak strain magnitude constant by altering strain *** experiment showed mat bone formation rate is directly proportional to strain *** on these experiments,Turner[5]showed that the strain stimulus （E） that determines bone response is proportional to strain magnitude and frequency:

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Laser-based in situ embedding of metal nanoparticles into bioextruded alginate hydrogel tubes enhances human endothelial cell adhesion

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Nano research 2016年第11期9卷 3407-3427页

作者： Andreas Blaeser Nina Million Daniela Filipa Duarte Campos Lisa Gamrad Marius Kopf Christoph Rehbock Milen Nachev Bernd Sures Stephan Barcikowski Horst Fischer Department of dental Materials and biomaterials research RWTH Aachen University Hospital Pauwelsstr. 30 52074 Aachen Germany Technical Chemistry I and Center for Nanointegration Duisburg-Essen (CENIDE) University of Duisburg-Essen Universitatsstr. 5-7 45141 Essen Germany Aquatic Ecology University of Duisburg-Essen Universitatsstr. 5 45141 Essen Germany Center for Water and Environmental research University of Duisburg-Essen Universitatsstr. 5 45141 Essen Germany

Alginate is a widely used hydrogel in tissue engineering owing to its simple and non-cytotoxic gelation process, ease of use, and abundance. However, unlike hydrogels derived from mammalian sources such as collagen, alginate does not contain cell adhesion Iigands. Here, we present a novel laser ablation technique for the in situ embedding of gold and iron nanoparticles into hydrogels. We hypothesized that integration of metal nanoparticles in alginate could serve as an alternative material because of its chemical biofunctionalization ability （coupling of RGD ligands） to favor cell adhesion. Cytocompatibility and biofunctionality of the gels were assessed by cell culture experiments using fibroblasts and endothelial cells. Nanoparticles with an average particle size of 3 nm （gold） and 6 nm （iron） were generated and stably maintained in alginate for up to 6 months. Using an extrusion system, several centimeter-long alginate tubes with an outer diameter of approximately 3 mm and a wall thickness of approximately 150 μm were manufactured. Confocal microscopy revealed homogeneously distributed nanoparticle agglomerates over the entire tube volume. Endothelial cells seeded on iron-loaded gels showed significantly higher viability and an increased degree of spreading, and the number of attached cells was also elevated in comparison to the control and gold-loaded alginates. We conclude that laser-based in situ integration of iron nanoparticles （40.01 wt.%） in alginate is a straightforward method to generate composite materials that favor the adhesion of endothelial cells. In addition, we show that nanoparticle integration does not impair the alginate＇s gelation and 3D biofabrication properties.

关键词： biofabrication tissue engineering HUVEC nanocomposite laser ablation confocal microscopy additive manufacturing

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Cone-beam computed tomography analysis of curved root canals after mechanical preparation with three nickel-titanium rotary instruments

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The Journal of Biomedical research 2013年第4期27卷 326-335页

作者： Samia M.Elsherief Mohamed K.Zayet Ibrahim M.Hamouda Department of Endodontics Faculty of Oral and Dental Medicine Cairo University Department of Endodontics Faculty of Dentistry Umm Al Qura University Lecturer of Radiology Faculty of Oral and Dental Medicine Cairo University Department of dental biomaterials Faculty of Dentistry Mansoura University Conservative Dentistry Department Faculty of Dentistry Umm Al Qura University

Cone beam computed tomography is a 3-dimensional high resolution imaging method. The purpose of this study was to compare the effects of 3 different NiTi rotary instruments used to prepare curved root canals on the final shape of the curved canals and total amount of root canal transportation by using cone-beam computed tomography. A total of 81 mesial root canals from 42 extracted human mandibular molars, with a curvature ranging from 15 to 45 degrees, were selected. Canals were randomly divided into 3 groups of 27 each. After preparation with Protaper, Revo-S and Hero Shaper, the amount of transportation and centering ability that occurred were assessed by using cone beam computed tomography. Utilizing preand post-instrumentation radiographs, straightening of the canal curvatures was determined with a computer image analysis program. Canals were metrically assessed for changes （surface area, changes in curvature and transportation） during canal preparation by using software SimPlant; instrument failures were also recorded. Mean total widths and outer and inner width measurements were determined on each central canal path and differences were statistically analyzed. The results showed that all instruments maintained the original canal curvature well with no significant differences between the different files （P=0.226）. During preparation there was failure of only one file （the protaper group）. In conclusion, under the conditions of this study, all instruments maintained the original canal curvature well and were safe to use. Areas of uninstrumented root canal wall were left in all regions using the various systems.

关键词： Rotary NiTi instrument canal curvature canal straightening

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The Influence of Ceramic Surface Treatments on the Microtensile Bond Strength of Resin Cements to Y-TZP Ceramic

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Journal of Wuhan University of Technology(Materials Science) 2010年第6期25卷 996-1000页

作者：朴美姬朴珠美 Seung-Geun Ahn Kwang-Yeob Song Tae-Sung Bae Jae-Youn Jung Department of Prosthodontics and Institute of Oral Bioscience School of DentistryChonbuk National University Department of dental biomaterials and Institute of Oral Bioscience School of DentistryChonbuk National University Division of Mechanical & Aerospace System Engineering and RCIT Chonbuk National University

The effects of surface conditioning methods on the microtensile bond strength of Y-TZP ceramic were studied based on airborne particle abrasion and resin *** square-shaped（φ12 mm×5 mm high） Y-TZP ceramic were studied blocks（LAVATM,3M ESPE,USA） and flat occlusal dentin blocks were fabricated,pre-treated（airborne abrasion with 125μm Al2O3 particles,tribochemical silica coating with 110 μm Al2O3 particles modified by silica oxide）,and bonded to each other using resin cements（Panavia F 2.0,RelyX Unicem）.Thereafter the trilayer specimens were cut into microbar specimens with a bonding area of approximately 1.0±0.1 mm2 and then microtensile bond strength tests were *** Y-TZP ceramic following airborne particle abrasion with 125μm Al2O3 and silicoating,the surface roughness of Y-TZP and its surface silica content were confirmed to ***,the Y-TZP ceramic surface treatment with a tribochemical silica coating showed the highest microtensile bond strength of the phosphate monomer-based resin cement to Y-TZP ceramic（mean MPa=18.11±0.27（Panavia F 2.0）,17.45±0.39（Rely X Unicem）.In cases in which a silica coating was applied,there was no significant difference in the bonding strength depending on resin cements（P0.05）.

关键词： Y-TZP ceramic resin cement microtensile bond strength

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Effect of surface mechanical attrition treatment of titanium using alumina balls： surface roughness, contact angle and apatite forming ability

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Frontiers of Materials Science 2013年第3期7卷 285-294页

作者： M. JAMESH T. S. N. SANKARA NARAYANAN Paul K. CHU II Song PARK Min Ho LEE National Metallurgical Laboratory Madras Centre CSIR Madras Complex Taramani Chennai 600 113 India Department of Physics and Material Science City University of Hong Kong Tat Chee Avenue Kowloon Hong Kong China School of Physics University of Sydney NSW 2006 Australia Department of dental biomaterials School of Dentistry Chonbuk National University Jeonju 561-756 South Korea

The effect of surface mechanical attrition treatment （SMAT） of commercially pure titanium （CP-Ti） using 8 mm O alumina balls was studied. SMAT induced plastic deformation, increased the surface roughness, reduced the grain size and decreased the contact angle （from 64° to 43°） with a corresponding increase in surface energy （from 32 to 53 mJ/m2）. Untreated CP-Ti and those treateded using alumina bails for 900 s reveals no apatite growth until the 28th day of immersion whereas those treated for 1800 and 2700 s exhibit apatite growth In selective areas and the extent of growth is increased with increase in immersion time an SBF; The study reveals that SMAT using alumina balls is beneficial in imparting the desired surface Cheracteristics, provided the surface contamination is limited, which would otherwise decrease the apatite forming ability.

关键词： KEYWORDS: nanostructured material surface modification sca.ning electron micro-scopy (SEM) surface mechanical attrition treatment(SMAT)

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Comparative evaluation of a biomimic collagen/ hydroxyapatite/β-tricaleium phosphate scaffold in alveolar ridge preservation with Bio-Oss Collagen

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Frontiers of Materials Science 2016年第2期10卷 122-133页

作者： Tong WANG Qing LI Gui-feng ZHANG Gang ZHOU Xin YU Jing ZHANG Xiu-mei WANG Zhi-hui TANG The 2nd dental Center Peking University School and Hospital of Stomatology Beijing 100101 China Center of Digital Dentistry Peking University School and Hospital of Stomatology Beijing 100081 China National Engineering Laboratory for Digital and Material Technology of Stomatology Beijing 100081 China Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 China Key Laboratory for biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical Engineering Beihang University Beijing 100191 China Institute of Regenerative biomaterials Tsinghua University Beijing 100084 China

Bone scaffolds are critical in current implant and periodontal regeneration approaches. In this study, we prepared a novel composite type-I collagen and hydroxyapatite （HA）/β-tricaleium phosphate （TCP） scaffold （CHTS） by incorporating type-I collagen and bovine calcined bone granules, prepared as a mixture of 50% HA and 50% TCP, by freeze drying. We then characterized the CHTS and determined its cytotoxic effects. Additionally, ridge preservation experiments were carried out to evaluate the clinical effects of the CHTS. The results demonstrated that the composite scaffolds had good surface morphology and no cytotoxicity. Additionally, an in vivo experiment in an animal model showed that the CHTS performed equally as well as Bio-Oss Collagen, a widely used bone graft in ridge preservation. These findings revealed that the CHTS, which contained natural constituents of bone, could be used as a scaffold for bone regeneration and clinical use.

关键词： hydroxyapatite β-tricaleium phosphate (TCP) collagen scaffold ridgepreservation

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Effect of water storage on fluoride release and mechanical properties of a polyacid-modified composite resin (compomer)

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The Journal of Biomedical research 2011年第4期25卷 254-258页

作者： Ibrahimm M.Hammouda Essam E.Al-Wakeel dental biomaterials Faculty of DentistryMansoura UniversityMansoura 35516Egypt Department of dental biomaterials Faculty of DentistryMansoura UniversityMansoura 35516Egypt

We evaluated the effect of water storage on fluoride release and mechanical properties of compomer restorative material. Fluoride release was recorded using a specific fluoride electrode. Flexural properties and fracture toughness were measured using a universal testing machine. Vickers hardness was measured using a micro-hardness tester. There was initial burst of fluoride release up to 1 w, which was diminished to a low level in 1 mon and remained relatively constant over 6 mon. Flexural strength and hardness were increased up to 1 mon followed by a gradual decrease up to 6 mon. Flexural modulus was decreased gradually up to 6 mon. Fracture toughness was increased during the first week and gradually decreased over the storage period. We concluded that flexural properties, fracture toughness, Vickers hardness and fluoride release of compomer were sensitive to water as well as storage time. There was a significant effect of fluoride release on the studied mechanical properties.

关键词： compomer restorative materials strength solubility

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Wireless electrical stimulation generated by piezoelectric nanomaterial promotes the dental pulp regeneration via regulating mitochondrial Ca^(2+)/PKA signaling pathway

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Journal of Materials Science & Technology 2024年第1期168卷 24-34页

作者： Jianmao Zheng Jiyuan Zuo Cairong Xiao Qining Guo Xiaobin Fu Chengyun Ning Peng Yu Guangdong Provincial Key Laboratory of Stomatology Hospital of StomatologySun Yat-Sen UniversityGuangzhou 510080China School of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhou 510641China

The induction of dental pulp stem cells(DPSCs)into odontogenic differentiation is a cutting-edge method of dental pulp regeneration ***,it remains a challenge to develop biomaterials and ther-apies that can induce odontogenic ***,we propose a wireless electrical stimulation strat-egy to induce DPSCs odontogenic differentiation via K_(0.5)Na_(0.5)NbO_(3)piezoelectric nanoparticles(KNN)and polarized KNN(PKNN),which can be endocytosed by *** vitro,several odontogenic differentiation indexes were also increased in DPSCs treated with KNN and PKNN,and the increase was more obvious in the PKNN *** wireless electrical field promoted mitochondrial calcium concentration via mitochondrial calcium unidirectional transporter(MCU),increased the production of adenosine triphos-phate(ATP),and induced odontogenic differentiation through the activation of the cAMP-PKA signaling *** vivo,dental pulp-like tissue was induced by electrical stimulation wirelessly with KNN and PKNN,which was more clinically friendly compared with the wired device,and the induction was more obvious in the PKNN group consistent with in vitro *** conclusion,this work demonstrates the potential of PKNN as an effective stimulus that can induce odontogenic differentiation of DPSCs and be applied to dental pulp regeneration treatment.

关键词： Piezoelectric nanoparticles dental pulp stem cells Odontogenic differentiation

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Preparation and application of highly porous aerogel-based bioactive materials in dentistry

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Frontiers of Materials Science 2014年第1期8卷 46-52页

作者： Andrea KUTTOR Melinda SZALOKI TLinde RENTE Farkas KERENYI Jozsef BAKO Istvan FABIAN Istvan LAZAR Attila JENEI Csaba HEGEDUES Department of Prosthetic Dentistry and biomaterials Faculty of Dentistry University of Debrecen 4012 Debrecen Hungary Aerogel research Group Department of Inorganic and Analytical Chemistry Faculty of Science University of Debrecen 4010 Debrecen Hungary Department of dental Biochemistry Faculty of Dentistry University of Debrecen 4012 Debrecen Hungary Department of Biophysics and Cell Biology Faculty of Medicine University of Debrecen 4012 Debrecen Hungary

In this study, the possibility of preparation and application of highly porous silica aerogel-based bioactive materials are presented. The aerogel was combined with hydroxyapatite and *** phosphate as bioactive and osteoinductive agents. The porosity of aerogels was in themesoporous region with a maximum pore diameter of 7,4 and 12.7 nm for the composite materials. The newly developed bioactive materials were characterized by scant electron microscopy. The in vitro biological effect of these modified surfaces was also tested on SAOS-2 osteogenic sarcoma cells by confocal laser scanning microscopy.

关键词： aerogel sol-gel technique bioactive matedal SAOS-2 cell

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