检索结果-南通市图书馆

TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2015年第7期42卷 383-392页

作者： Yahu Gao Dongzhi Zhang Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou University

Our previous studies indicated that TCP1 is a positive regulator of the brassinosteroid （BR） biosynthesis pathway by mediating the transcription of DWF4, one of the key BR biosynthetic genes in Arabidopsis thaliana. Whether TCP1 can directly bind to the promoter region of DWF4, however, has not been experimentally demonstrated. Here we provide our biochemical and genetic evidence that TCP1 mediates the expression of DWF4 by directly associating with the two GGNCCC motifs in the promoter region of DWF4. The expression levels of DWF4 are positively correlated to TCP1 abundance in planta. Electrophoretic mobility shift assays （EMSAs） using various synthetic DNA fragments suggest that the GGNCCC core sequence is critical for TCP1 binding. DNA sequences flanking the GGNCCC motifs are less important for the association of TCPI. Using DWF4p-GUS transgenic plants as an assay system, it is clearly indicated that these motifs are required for the positive regulation of DWF4 transcription by TCP1. More significantly, whole genome microarray analyses indicate that TCP1 can directly or indirectly regulate the expression of many other genes known to be important for normal plant growth and development.

关键词： Brassinosteroids TCP1 DWF4 Arabidopsis Homeostasis

Arabidopsis ROOT ELONGATION RECEPTOR KINASES negatively regulate root growth putatively via altering cell wall remodeling gene expression

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第8期64卷 1502-1513页

作者： Yanze Wang Weiyue Chen Yang Ou Yingying Zhu Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China State key laboratory of Grassland Agro‐Ecosystems Institute of Innovation EcologyLanzhou UniversityLanzhou 730000China Guangdong Provincial key laboratory of Plant Adaptation and Molecular Design School of Life SciencesGuangzhou UniversityGuangzhou 510006China

Receptor-like kinases(RLKs)play key roles in regulating various physiological aspects in plant growth and *** Arabidopsis thaliana,there are at least 223 leucine-rich repeat(LRR)*** functions of the majority of RLKs in the LRR XI subfamily were previously *** three RLKs were not *** we report that two independent triple mutants of these RLKs,named ROOT ELONGATION RECEPTOR KINASES(REKs),exhibit increased cell numbers in the root apical meristem and enhanced cell size in the elongation and maturation *** promoter activities of a number of Quiescent Center marker genes are significantly up-regulated in the triple ***,the promoter activities of several marker genes known to control root stem cell niche activities are not ***-seq analysis revealed that a number of cell wall remodeling genes are significantly up-regulated in the triple *** results suggest that these REKs play key roles in regulating root development likely via negatively regulating the expression of a number of key cell wall remodeling genes.

关键词： Arabidopsis thaliana cell expansion cell wall receptor-like kinase REK root

Receptor-like protein kinases:key regulators controlling root hair development in Arabidopsis thaliana

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2018年第9期60卷 841-850页

作者： Zhuoyun Wei Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Root hairs are tubular outgrowths specifically differentiated from epidermal cells in a differentiation zone. The formation of root hairs greatly increases the surface area of a root and maximizes its ability to absorb water and inorganic nutrients essential for plant growth and development. Root hair development is strictly regulated by intracellular and intercellular signal communications. cell surface-localized receptor-like protein kinases （P, LKs） have been shown to be important components in these cellular processes, tn this review, the functions of a number of key P, LKs in regulating Arabidopsis root hair development are discussed, especially those involved in root epidermal cell fate determination and root hair tip growth.

关键词： Receptor-like protein kinases:key regulators controlling root hair development in Arabidopsis thaliana

Brassinosteroids Regulate Root Growth, Development, and Symbiosis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2016年第1期9卷 86-100页

作者： Zhuoyun Wei Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life Sciences Lanzhou University Lanzhou 730000 China

Brassinosteroids （BRs） are natural plant hormones critical for growth and development. BR-deficient or signaling mutants show significantly shortened root phenotypes. But for a long time, it was thought that these phenotypes were solely caused by reduced root cell elongation in the mutants. Functions of BRs in regulating root development have been largely neglected. Recent detailed analyses, however, revealed that BRs are not only involved in root cell elongation but are also involved in many aspects of root development, such as maintenance of meristem size, root hair formation, lateral root initiation, gravitropic response, mycorrhiza formation, and nodulation in legume species. In this review, current findings on the functions of BRs in mediating root growth, development, and symbiosis are discussed.

关键词： brassinosteroids root meristem root hair lateral root development gravitropic response symbiosis

Protein farnesylation negatively regulatesbrassinosteroid signaling via reducing BES1 stabilityinArabidopsis thaliana^(∞)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2021年第7期63卷 1353-1366页

作者： Zengxiu Feng Hongyong Shi Minghui Lv Yuang Ma Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a *** Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and *** famesylation is a type of post-translational modification,functioning in many important cellular *** studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme *** such a process is also involved in BR signaling is not un***,we demonstrate that protein famesylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR *** genetic and biochemical analyses revealed that ERA1 plays a significant role in regulating the protein stability of BES1.

关键词： Arabidopsis BES1 brassinolide brassinosteroids protein famesylation

Regulation of Brassinosteroid Biosynthesis and Inactivation

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2012年第10期54卷 746-759页

作者： Baolin Zhao Jia Li key laboratory of cell activities and stress adaptations Ministry of EducationSchool of Life SciencesLanzhou UniversityLanzhou 730000China

Brassinosteroids （BRs） are a group of naturally-occurring steroidal phytohormones playing fundamental roles during normal plant growth and development. Using a combination of experimental approaches, including analytical chemistry, genetics, and biochemistry, the major BR biosynthetic pathway has been largely elucidated. The least-understood knowledge in the BR research field is probably the molecular mechanisms controlling the bioactive levels of BRs in response to various developmental and environmental cues. In this review, we focus our discussion on a recently-proposed, 8-step predominant BR biosynthetic pathway, several newly-identified transcription factors regulating the expression of key enzymes that catalyze BR biosynthesis, and up-to-date information about the mechanisms that plants use to inactivate unnecessary BRs.

关键词： Brassinolide brassinosteroid biosynthesis homeostasis catabolism.

RGF1-RGI1,a Peptide-Receptor Complex,Regulates Arabidopsis Root Meristem Development via a MAPK Signaling Cascade

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2020年第11期13卷 1594-1607页

作者： Xiaoting Lu Hongyong Shi Yang Ou Yanwei Cui Jinke Chang Liang Peng Xiaoping Gou Kai He Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

Root growth is maintained by the continuous division of cells in the apical *** MERISTEM GROWTH FACTOR 1(RGF1)is a critical peptide hormone regulating root stem cell niche *** studies discovered that five closely related leucine-rich repeat receptor-like protein kinases(LRRRLKs),named RGF1 INSENSITIVES(RGIs)or RGF1 RECEPTORS(RGFRs),are able to perceive the RGF1 signal and redundantly control root stem cell niche ***1 regulates root meristem activity mainly via two downstream transcription factors,PLETHORA 1(PLT1)and *** proteins connecting cell surface RGF1-RGI1 and nuclear PLTs,however,were not ***,we report that the mitogen-activated protein(MAP)kinase kinase 4(MKK4)and MAP kinase 3(MPK3)were coimmunoprecipitated with RGI1-FLAG after Arabidopsis seedlings were treated with *** and biochemical assays confirmed that MKK4 and MKK5,and their downstream targets MPK3 and MPK6,are essential RG卜dependent regulators of root meristem *** addition,we found that the MKK4/MKK5-MPK3/MPK6 module functions downstream of YDA,a *** results demonstrate that RGF1-RGI1 regulate the expression of PLT1/PLT2 via a YDA-MKK4/MKK5-MPK3/MPK6 signaling cascade.

关键词： root meristem RGF1 RGI1 RLK MAPK

Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2021年第7期14卷 1119-1134页

作者： Yafen Zhu Chong Hu Yanwei Cui Li Zeng Sunjingnan Li Mingsong Zhu Fanhui Meng Shuting Huang Li Long Jing Yi Jia Li Xiaoping Gou ministry of education key laboratory of cell activities and stress adaptations School of Life SciencesLanzhou UniversityLanzhou 730000China

The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,*** CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell ***,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be ***,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root *** found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)*** assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in *** addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on *** CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems.

关键词： ACR4 CIKs CLE40 stem cells distal root meristem phosphorylation

Genome-Wide Expression Pattern Analyses of the Arabidopsis Leucine-Rich Repeat Receptor-Like Kinases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2016年第2期9卷 289-300页

作者： Yunzhe Wu Qingqing Xun Yi Guo Jinghua Zhang Kaili Cheng Tao Shi Kai He Suiwen Hou Xiaoping Gou Jia Li ministry of education key laboratory of cell activities and stress adaptations School of Life Sciences Lanzhou University Lanzhou 730000 China

Receptor-like protein kinases （RLKs） are a large group of transmembrane proteins playing critical roles in cell-cell and cell--environment communications. Based on extracellular domain structures, RLKs were classified into more than 21 subfamilies, among which leucine-rich repeat RLKs （LRR-RLKs） belong to the largest subfamily in plants such as Arabidopsis and rice. In Arabidopsis, there are approximately 223 LRR-RLKs, but only about 60 of which have been functionally described to date. To systematically investigate the roles of LRR-RLKs in regulating plant growth, development, and stress adaptations, we generated promoter：：GUS transgenic plants for all 223 LRR-RLK genes in Arabidopsis and analyzed their detailed expression patterns at various developmental stages. The results provide valuable resources for functionally elucidating this large and essential signaling protein subfamily.

关键词： receptor-like kinase RLK LRR-RLK Arabidopsis expression pattern