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Asian Pacific Journal of Tropical Medicine 2012年第9期5卷 718-721页

作者： Varsha Gupta Shailpreet Sidhu Jagdish Chander Department of microbiology CovtMedical College & HospitalSector-32ChandigarhIndia

Objective:To detect and evaluate the various methods for metallo-β-lactamases(MBL) production in Pseudomonas aeruginosa(***) and Acinetobacter ***:A total of 109 *** and 85 Acinetobacter species were screened for imipenem resistance by Kirby- Bauer disc diffusion *** of MBL production was(lone by imipenem-EDTA combined disc test,double disc synerygy test(DDST) and imipenem-EDTA MBL E ***: A total of 63(57.8%) strains of *** and 46(54.1%) strains of Acinetobacter *** found to be resistant to *** the 63 imipenem resistant *** tested for MBL production.44(69.89;) were found to be positive and among 46 imipenem resistant Acinetobacter. 19(41.3%) were shown to be the MBL ***:Imipenem-EDTA combined disc test and MBL E test are equally effective for MBL detection in both *** and Acinetobacter spp.,but given the cost-constraints,combined disc can be used as a convenient screening method in the clinical microbiology laboratory.

关键词： Carbapenem-imipenem Metallo-β-lactamases Non fermentative gram-negative bacilli

Successful immunological treatment of gallbladder cancer in India―Case report

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第9期7卷 719-724页

作者： KHAN Jamal A. YAQIN Sharmin microbiology Research Lab C-38/sector 20 Noida Zip 201301 India

Gallbladder cancer has a poor outcome because of its anatomy and location. Often, the diagnosis is made very late due to its silent course. Post-operated cases do respond to chemotherapy but the survival is counted in months and the quality of life is further hampered due to toxicity of drugs. Immunotherapy holds good promise in non-responding cancers treated by conventional chemotherapeutic agents. Among various therapies, dendritic cell therapy is growing at rapid pace due to its acceptable rationale. It has been utilized in treating successfully resected stage Ill （T2, N1, M0） gallbladder cancer in one of our patients. A 48 years old lady treated with this therapy is free of metastasis with ten doses of autologous dendritic cell vaccine constructed by utilizing resected tumor lysate antigen. She has received ten doses of therapy in 14 months of her treatment. This therapy has proven to be safe and without apparent side effects. The positive clinical response obtained supports that autologous dendritic cell-based immunotherapy is a promising therapeutic approach for refractory gallbladder cancers.

关键词： Gallbladder Cancer Dendritic cell Immunotherapy

Dendritic cell therapy with improved outcome in glioma multiforme―a case report

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第2期7卷 114-117页

作者： KHAN Jamal A. YAQIN Sharmin microbiology Research Lab C-38/Sector 20 Noida (U.P.) 201301 India

Malignant gliomas are the most devastating tumors in clinical practice and nave poorest survival, Immunological treatment of such patients may likely increase the survival and quality of life. Dendritic cells （DCs）, most potent antigen presenting cells in combination with oral chemotherapeutic agents may be tried for patients giving consent to such treatment. We have successfully combined the two therapies in an adult male patient who was on downhill course after being operated on once with post operation chemotherapy and radiotherapy for glioma in the left parietal area. He received five dendritic cell therapy vaccines in combination with oral chemotherapy and responded dramatically having near normal quality of life for an additional five months with this regime, increasing the survival after operation to 11 months. This therapy is continuing with radiological betterment of the lesion. The DCs are matured with antigen extracted from wax embedded tissue at 6th day of culture. We feel that the treatment can be given to more number of patients to establish its efficacy for the dreaded cancer glioblastoma multiforme.

关键词： Dendritic cells Glioma Immunetherapy

A simple and purification-free nucleic acid extraction method for rapid diagnosis of malaria

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Asian Pacific Journal of Tropical Medicine 2023年第9期16卷 428-430页

作者： Lee Phone Youth Zen Meng Yee Lai Mohd Hafizi Abdul Hamid Jenarun Jelip Rose Nani Mudin Noor Hafizan Bt Mat Salleh Netty Darwina Dawam Nirzayanna Eyan Abdul Ajak Mohd Ashrin Afiq Bin Zainudin Yee Ling Lau Department of Parasitology Faculty of MedicineUniversiti Malaya50603 Kuala LumpurMalaysia Vector Borne Disease sector Ministry of HealthPutrajayaMalaysia Lipis District Health Office PahangMalaysia State Health Department Negeri SembilanMalaysia microbiology sector Hospital Kuala Kubu BharuSelangorMalaysia

In a point of care setting for the diagnosis of malaria,DNA extraction using conventional methods are time-consuming and ***,in this study we aim to utilize a simple nucleic acid extraction method to directly extract DNA from *** would in turn reduce the time,cost and equipment needed to perform DNA *** method is then coupled with LAMP assay for rapid diagnosis of malaria.

关键词： diagnosis malaria utilize

Maternal salinity improves yield, size and stress tolerance of Suaeda fruticosa seeds

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Journal of Arid Land 2020年第2期12卷 283-293页

作者： Syed Z SHAH Aysha RASHEED Bilquees GUL Muhammad A KHAN Brent LNIELSEN Abdul HAMEED Institute of Sustainable Halophyte Utilization University of KarachiKarachi 75270Pakistan Govt.Degree Boys College Sector 4ENew SaeedabadBaldia TownKarachi 75760Pakistan Department of microbiology&Molecular Biology Brigham Young UniversityProvoUT 84602USA

Shrubby seablite or lani(Suaeda fruticosa Forssk)is a perennial euhalophyte with succulent leaves,which could be planted on arid-saline lands for restoration and cultivated as a non-conventional edible or cash *** about the impacts of maternal saline environment on seed attributes of this important euhalophyte is *** study investigated the effects of maternal salinity on yield,size and stress tolerance of *** *** of *** were grown in a green net house under increasing maternal salinity levels(0,300,600 and 900 mM NaCl)until seed *** yield,size,stress tolerance and germination of the descended seeds under different maternal saline conditions were *** grown under saline conditions(300,600 and 900 mM NaCl)produce a substantially higher quantity of seeds than plants grown under non-saline condition(0 mM NaCl).Low maternal salinity(300 mM NaCl)improves seed *** produced under all maternal salinity levels display a higher tolerance to low temperature(night/day thermoperiod of 10℃/20℃),whereas seeds produced under 300 mM NaCl maternal saline condition show a better tolerance to high temperature(night/day thermoperiod of 25℃/35℃)during *** from all maternal saline conditions germinate better in the 12 h photoperiod(12 h light/12 h dark)than in the dark(24 h dark);however,seeds produced from low and moderate maternal saline conditions(300 and 600 mM NaCl)show a higher germination in the dark than those from control and high maternal saline conditions(0 and 900 mM NaCl).In general,maternal salinity is found to improve yield,size and stress tolerance of *** seeds.

关键词： euhalophyte maternal salinity salt tolerance seed germination non-saline condition Suaeda fruticosa

Science： a world without borders

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Protein & Cell 2015年第8期6卷 551-552页

作者： Joel Haywood CAS Key Laboratory of Pathogenic microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China

In 2010 the effects of the global financial crisis were still being felt in the UK. Stock market confidence levels were low, unemployment levels were high and job opportunities were few and far between especially for new graduates with little work experience. Unfortunately for my partner, her recent graduation and search for job opportunities within the finance and law sector could not have come at a more unsuitable time. China however had better weathered the storm of the financial crisis and presented itself as a more familiar and fruitful climate for my partner to further develop her career. Furthermore.

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Correlation between human gut microbiome and diseases

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Infectious Medicine 2022年第3期1卷 180-191页

作者： Barkha Madhogaria Priyanka Bhowmik Atreyee Kundu Department of microbiology Techno India UniversityWest Bengal EM-4 Sector-VSalt Lake CityKolkata 700091West BengalIndia Department of Biological Sciences Adamas UniversityBarrackpore-Barasat Road24 Paragnas NorthJagannathpurKolkataWest BengalIndia

Human gut microbiome is a major source of human bacterial population and a significant contribution to both positive and harmful *** to its involvement in a variety of interactions,gut microorganisms have a great impact on our health throughout our *** impact of gut microbial population is been studied intensively in last two *** literature survey focusing developments in the field were searched in English language Electronic Databases like PubMed,Google Scholar,Pubag,Google books,and Research Gate were mostly used to understand the role of human gut mirobiome and its role in different human *** microbiome in healthy subjects differs from those who suffer from *** 2 diabetes,obesity,non-alcoholic liver disease,and cardiometabolic diseases have all been linked to dysbiosis of the gut *** of many disorders is also linked to changes in gut *** diseases like cancer,arithritis,autism,depression,anxiety,sleep disorder,HIV,hypertension,and gout are also related to gut microbiota *** focus in this review on recent studies looking into the link between gut microbiome dysbiosis and disease *** on how gut microbiota affects host metabolism has been changed in past decades from descriptive analyses to high throughput integrative omics data analysis such as metagenomics and *** of molecular mechanisms behind reported associations is been carried out in human,animals,and cells for measure of host physiology and *** many the mechanisms are not completely understood.

关键词： Bacteria Gut Infections Microbiome Microbiota Microflora

Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean

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Pedosphere 2020年第1期30卷 98-108页

作者： Linnajara DE VASCONCELOS MARTINS FERREIRA Fernanda DE CARVALHO Júlia FONSECA COLOMBO ANDRADE Damiany PáDUA OLIVEIRA Flávio Henrique VASCONCELOS DE MEDEIROS Fatima Maria DE SOUZA MOREIRA sector of Biology Microbiology and Biological ProcessesDepartment of Soil ScienceUniversidade Federal de LavrasLavras C.P.3037-37200-000(Brazil) Instituto Federal do Pará/IFPA Campus MarabáRuralMarabáC.P.041-68508-979(Brazil) Universidade Federal de Lavras Department of PhytopathologyLavras C.P.3037-37200-000(Brazil)

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia ***,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase *** this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield *** aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean *** experiments were conducted under axenic conditions using the common bean cultivar Pé*** first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of *** second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common ***-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping *** the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/*** endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial i

关键词： Bacillus sp. biological control Burkholderia fungorum CIAT 899 Phaseolus vulgaris plant growth-promoting rhizobacteria Pseudomonas sp. Rhizoctonia solani

Eminence of Microbial Products in Cosmetic Industry

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Natural Products and Bioprospecting 2019年第4期9卷 267-278页

作者： Prabhuddha LGupta Mahendrapalsingh Rajput Tejas Oza Ujwalkumar Trivedi Gaurav Sanghvi Department of microbiology Marwadi UniversityRajkot 360001India

Cosmetology is the developing branch of science,having direct impact on the *** cosmetic sector is interested in finding novel biological alternatives which can enhance the product attributes as well as it can substitute chemical *** of the compounds are having biological origin and are acquire from bacteria,fungi,and algae.A range of biological compounds,like bio-surfactant,vitamins,antioxidants,pigments,enzymes,peptides have promising features and beneficial ***,these products can be produced commercially with *** review will encompass the importance and use of microbial compounds for new cosmetic formulations as well as products associated with it.

关键词： Cosmetics microbiology Biosurfactants Formulations Cyclodextrin Emulsions

Production and Partial Characterization of an Extracellular Phytase Produced by Muscodor sp. under Submerged Fermentation

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Advances in microbiology 2016年第1期6卷 23-32页

作者： Natálie Martins Alves Luis Henrique Souza Guimarães Roberta Hilsdorf Piccoli Patrícia Gomes Cardoso Department of Biology Sector of Microbiology Federal University of Lavras Lavras Brazil Department of Biology University of Sao Paulo Ribeirao Preto Brazil Department of Food Science Federal University of Lavras Lavras Brazil

In most of the raw materials of plant origin used in animal feed, a portion of the phosphorus is stored as phytic acid or phytate. Phytate is the main storage form of phosphorus in vegetables but is not readily assimilated into food at low concentrations of the enzyme phytase. In addition to making phosphorous unavailable, phytate binds divalent cations such as calcium, copper, magnesium, iron, manganese and zinc, preventing the absorption of these nutrients in the gut of the animal. Phytase promotes the hydrolysis of the phytate phosphorus-releasing molecule, thereby increasing its bioavailability in feed. Phytase is distributed in plant and animal tissues and it is synthesized by some species of bacteria and fungi. The addition of this enzyme in the diet of animals is essential to promote greater uptake of phosphorus and also contributes to a decrease in the levels of phosphorus excreted by animals, thus reducing the pollution caused by excess phosphorus in the environment. This work aimed to select a fungus that stands out in the production of phytase among 100 isolates from Brazilian caves belonging to the genera Aspergillus, Penicillium and Cladosporium and 13 endophytic fungi of the aerial part of the coffee plant. For selection, the fungi were cultured in medium containing phytic acid as a sole source of phosphorus. After seven days at 25 °C, we evaluated growth and enzyme production by the presence of the phytic acid halo degradation (Enzymatic Index-EI) surrounding the colonies. Forty-seven species produced phytase, and the fungi Penicillium minioluteum (CF279) and Muscodor sp. (UBSX) showed higher degradation halos, 2.41 and 4.46, respectively. Considering the Muscodor sp. as the main source of phytase, high enzymatic levels were obtained when the fungus was grown under submerged fermentation with initial pH of 5.0 using wheat bran as additional carbon source for 144 h, at 125 rpm and 30 °C. Additionally, the enzyme was stable at pH 5.0 and 40 °C, and inhi

关键词： Endophytic Fungus Muscodor sp. Phosphorus Phytase Phytic Acid