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Journal of Environmental Sciences 2007年第3期19卷 367-373页

作者： SHIGWEDHA Nditange HUA Zhao-zhe CHEN Jian Laboratory of Environmental biotechnology School of Biotechnology Southern Yangtze University Wuxi 214036 China Key Laboratory of Industrial biotechnology Ministry of Education Southern Yangtze University Wuxi 214036 China

Actual textile wastewater and synthesized wastewater containing various textile dyes were photocatalytic degraded by the UVH2O2Fs-TiO2 process in an aimular-flow photocatalytic reactor. In this process, a photon kinetic-measure was adopted to obtain constant rates of dyes decomposition. It was theorized that, by illumination at different UV frequencies, the electrons within the semiconductor were excited from the valence band to the conduction band, yielding the formation of electron-hole pairs which are the pre-requisites for photocatalysis. CPT （critical photonic time） exposure required to cause 90% of vibrations between the double and single bonds along the molecular chain of the dyes to be oxidized, was taken to measure the photocatalytic activities. The CPTs varied with the frequencies of the UV spectral areas. The derivatization of CPT from the first-order kinetic law was presented.

关键词： critical photonic time (CPT) photon efficiency UV frequencies regression line textile wastewater

Recent progress of on-resin cyclization for the synthesis of clycopeptidomimetics

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Chinese Chemical Letters 2016年第12期27卷 1731-1739页

作者： Zhi-Meng Wu Shao-Zhong Liu Xiao-Zhong Cheng Wen-Zhang Ding Tao Zhu Bing Chen The Key Laboratory of Carbohydrate Chemistry & biotechnology Ministry of Education School of Biotechnology Jiangnan University

Cyclopeptidomimetics are class of cyclopeptides with unnatural linkage. They usually displayed unique constrained structure, enhanced proteolytic stability, and other drug-like character; and have been widely used in medicinal chemistry. Therefore, development of efficient strategies for the synthesis of cyclopeptidomimetics has received many attentions. On-resin cyclization strategy is one of the effective approaches developed to overcome the competing side reaction such as oligomerization and cyclooligomers occurred in solution cyclization. This approach took advantage of the ＂pseudo-dilution＂ effect to avoid these undesired by-products and greatly simplified the downstream product purification process. This review summarized the recent on-resin peptide cyclization strategies for the synthesis of cvclooeotidomimetics.

关键词： Cyclopeptidomimetics On-resin Cyclization Synthesis

Insight into the distribution of metallic elements in membrane bioreactor: Influence of operational temperature and role of extracellular polymeric substances

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Journal of Environmental Sciences 2019年第2期31卷 111-120页

作者： He Wang Xiufen Li Xinhua Wang Yueping Ren Laboratory of Environmental biotechnology School of Environmental and Civil EngineeringJiangnan University Jiangsu Key Laboratory of Anaerobic biotechnology

The distribution of metallic elements in a submerged membrane bioreactor(MBR) was revealed at different temperatures using inductively coupled plasma-optical emission spectrometry(ICP-OES), and the role of extracellular polymeric substances(EPS) was probed by integrating scanning electron microscopy(SEM) with confocal laser scanning microscopy(CLSM) over long-term operation. More metallic elements in the influent were captured by suspended sludge and built up in the fouling layer at lower temperature. The concentration of metallic elements in the effluent was 5.60 mg/L at 10°C operational temperature, far lower than that in the influent(51.35 mg/L). The total contents of metallic elements in suspended sludge and the membrane fouling layer increased to 40.20 and 52.19 mg/g at 10°C compared to 35.14 and 32.45 mg/g at 30°C, and were dominated by the organically bound fraction. The EPS contents in suspended sludge and membrane fouling layer sharply increased to 37.88 and 101.51 mg/g at 10°C, compared to 16.87 and 30.03 mg/g at 30°C. The increase in EPS content at lower temperature was responsible for the deposition of more metallic ions. The strong bridging between EPS and metallic elements at lower temperature enhanced the compactness of the fouling layer and further decreased membrane flux. This was helpful for understanding the mechanism of membrane fouling at different operational temperatures and the role of EPS, and also of significance for the design of cleaning strategies for fouled membranes after long-term operation.

关键词： Membrane bioreactor Metallic elements Extracellular polymeric substances

Novel microbial fuel cell design to operate with different wastewaters simultaneously

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Journal of Environmental Sciences 2016年第4期28卷 105-111页

作者： Abhilasha Singh Mathuriya Department of biotechnology Anand Engineering College

A novel single cathode chamber and multiple anode chamber microbial fuel cell design（MAC-MFC）was developed by incorporating multiple anode chambers into a single unit and its performance was *** 60 days of operation,performance of MAC-MFC was assessed and compared with standard single anode/cathode chamber microbial fuel cell（SC-MFC）.The tests showed that MAC-MFC generated stable and higher power outputs compared with SC-MFC and each anode chamber contributed ***,MAC-MFCs were incorporated with different wastewaters in different anode chambers and their behavior in MFC performance was ***-MFC efficiently treated multiple wastewaters simultaneously at low cost and small space,which claims its candidature for future possible scale-up applications.

关键词： Microbial fuel cells Multiple wastes Bioelectricity New design Wastewater treatment

Isolation of a salt tolerant laccase secreting strain of Trichoderma sp. NFCCI-2745 and optimization of culture conditions and assessing its efectiveness in treating saline phenolic efuents

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Journal of Environmental Sciences 2013年第12期25卷 2410-2416页

作者： L.M.Divya G.K.Prasanth C.Sadasivan Department of biotechnology and Microbiology Kannur University

Most of the hazardous pollutants are phenolic in nature and persists in the environment. The ability of laccases to oxidize phenolic compounds and reduce molecular oxygen to water has led to intensive studies of these enzymes. Therefore the fungal strains with high laccase activity and substrate affinity that can tolerate harsh environmental conditions have a potential for biotechnological applications. Salt tolerant laccase secreting fungi can be utilized in treatment of saline and phenolic rich industrial effluents such as coir effluent and textile effluent that needed to be diluted several fold before microbial treatment. This is the first study describing the isolation and optimization of a salt tolerant strain of Trichoderma sp. potential for industrial applications. The fungus was identified based on morphological characteristics and was subsequently confirmed with molecular techniques and deposited at National Fungal Culture Collections of India （NFCCI） under the Accession No. Trichoderma viride NFCCI 2745. In contrast to other laccase secreting fungi, light conditions did not exert much influence on laccase production of this strain and salinity enhanced its laccase secretion. The fungus effectively removed the phenolic content of the textile effluent, coir-ret liquor and wood processing effluent within 96 hr of incubation. The tolerance of the fungus to high salinity and phenolic compounds makes this strain ideal for treating saline and phenolic rich industrial effluents.

关键词： laccase optimization phenol tolerant salt tolerant Trichoderma viride

Complete genome sequence of Bacillus amyloliquefaciens YP6, a plant growth rhizobacterium efficiently degrading a wide range of organophosphorus pesticides

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Journal of Integrative Agriculture 2019年第11期18卷 2668-2672页

作者： MENG Di ZHAI Li-xin TIAN Qiao-peng GUAN Zheng-bing CAI Yu-jie LIAO Xiang-ru Key Laboratory of Industrial biotechnology Ministry of Education School of Biotechnology Jiangnan University

Bacillus amyloliquefaciens YP6,a plant growth promoting rhizobacteria,is capable of efficiently degrading a wide range of organophosphorus pesticides(OPs).Here,we report the complete genome sequence of this bacterium with a genome size of 4009619 bp,4210 protein-coding genes and an average GC content of 45.9%.Based on the genome sequence,several genes previously described as being involved in solubilizing-phosphorus,OPs-degradation,indole-3-acetic acid(IAA)and siderophores ***,compared with the genomes of *** species,strain YP6 had larger genome size and the most protein-coding ***,the four categories of“cell envelope biogenesis,outer membrane(M),”“translation,ribosomal structure and biogenesis(J),”“transcription(K),”and“signal transduction mechanisms(T)”were *** differences may be related to extensive environmental adaptability of the genus *** results expand the application potential of strain YP6 for environmental bioremediation,provide gene resources involved in OPs degradation for biotechnology and gene engineering,and contribute to provide insights into the relationship between microorganism and living environment.

关键词： organophosphorus pesticides genome sequence plant growth promoting rhizobacteria Bacillus amyloliquefaciens

Food components and diet habits:chief factors of cancer development

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Food Quality and Safety 2019年第4期3卷 227-231页

作者： Rihab Ksouri Department of biotechnology Biotechnology InstituteAnkara UniversityTurkey

Food is a vital need for ***,there is food for all,but the world still suffers from under-and over-nutrition and risk of cancer development and chronic diseases can follow both ***,cancer is a leading cause of mortality after cardiovascular disease;it is considered the second reason for death *** of nutritional habits,the quality of food,the consumption of canned foods,genetically modified fruits and vegetables and exposed food to certain pesticides and carcinogens agents,and unhealthy lifestyle behaviours such as smoking,alcohol,obesity,and fast-foods consumption may be at risk to the development of some *** recent decades,researchers have carried out attention in this field to improve the quality of life and to limit nutrition ***,this study aims to summarize current evidence on the relationship between nutritional factors and cancer expansion,how nutrition can be a heal and a source of fatal illness leading to *** detail,this review will highlight the influence of specific foodstuffs on the threat of cancer incidence and recurrence by providing some examples of most carcinogenic compounds.

关键词： cancer carcinogens food nutritional habits

An Effective Integrated Machine Learning Framework for Identifying Severity of Tomato Yellow Leaf Curl Virus and Their Experimental Validation

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Research 2023年第3期2023卷 91-104页

作者： Nattanong Bupi Vinoth Kumar Sangaraju Le Thi Phan Aamir Lal Thuy Thi Bich Vo Phuong Thi Ho Muhammad Amir Qureshi Marjia Tabassum Sukchan Lee Balachandran Manavalan Department of Integrative biotechnology College of Biotechnology and BioengineeringSungkyunkwan UniversitySuwon 16419.Gyeonggi-doRepublic of Korea Computational Biology and Bioinformatics Laboratory Department of Integrative BiotechnologyCollege of Biotechnology and Bioengineering.Sungkyunkwan UniversitySuwon 16419Gyeonggi-doRepublic of Korea

Tomato yellow leaf curl virus(TYLCV)dispersed across different countries,specifically to subtropical regions,associated with more severe *** TYLCV was first isolated in 1931,it has been a menace to tomato industrial production worldwide over the past *** groups were newly isolated from TYLCV-resistant tomatoes in 2022;however,their functions are *** development of machine learning(ML)-based models using characterized sequences and evaluating blind predictions is one of the major challenges in interdisciplinary *** purpose of this study was to develop an integrated computational framework for the accurate identification of symptoms(mild or severe)based on TYLCV sequences(isolated in Korea).For the development of the framework,we first extracted 11 different feature encodings and hybrid features from the training data and then explored 8 different classifiers and developed their respective prediction models by using randomized 10-fold ***,we carried out a systematic evaluation of these 96 developed models and selected the top 90 models,whose predicted class labels were combined and considered as reduced *** the basis of these features,a multilayer perceptron was applied and developed the final prediction model(IML-TYLCVs).We conducted blind prediction on 3 groups using IML-TYLCVs,and the results indicated that 2 groups were severe and 1 group was ***,we confirmed the prediction with virus-challenging experiments of tomato plant phenotypes using infectious clones from 3 *** virologists and plant breeding professionals can access the user-friendly online IML-TYLCVs web server at https://***/IML-TYLCVs,which can guide them in developing new protection strategies for newly emerging viruses.

关键词： breeding fir prediction

Sclerotinia sclerotiorum virulence is affected by mycelial age via reduction in oxalate biosynthesis

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Journal of Integrative Agriculture 2016年第5期15卷 1034-1045页

作者： WANG Ji-peng XU You-ping ZANG Xian-peng LI Shuang-sheng CAI Xin-zhong Institute of biotechnology College of Agriculture and Biotechnology Zhejiang University Center of Analysis and Measurement Zhejiang University

Sclerotinia sclerotiorum is one of the most devastating necrotrophic phytopathogens. Virulence of the hyphae of this fungus at different ages varies significantly. Molecular mechanisms underlying this functional distinction are largely unknown. In this study, we confirmed the effect of mycelial culture time/age on virulence in two host plants and elucidated its molecular and morphological basis. The virulence of the S. sclerotiorum mycelia in plants dramatically decreases along with the increase of the mycelial age. Three-day-old mycelia lost the virulence in plants. Comparative proteomics analyses revealed that metabolism pathways were comprehensively reprogrammed to suppress the oxalic acid（OA） accumulation in old mycelia. The oxaloacetate acetylhydrolase（OAH）, which catalyzes OA biosynthesis, was identified in the S. sclerotiorum genome. Both gene expression and protein accumulation of OAH in old mycelia were strongly repressed. Moreover, in planta OA accumulation was strikingly reduced in old mycelia-inoculated plants compared with young vegetative mycelia-inoculated plants. Furthermore, supply with 10 mmol L^（-1） OA enabled the old mycelia infect the host plants, demonstrating that loss of virulence of old mycelia is mainly caused by being unable to accumulate OA. Additionally, aerial mycelia started to develop from 0.5-day-old vegetative mycelia and dominated over 1-day-old mycelia grown on potato dextrose agar plates. They were much smaller in hypha diameter and grew significantly slower than young vegetative mycelia when subcultured, which did not maintain to progenies. Collectively, our results reveal that S. sclerotiorum aerial hyphae-dominant old mycelia fail to accumulate OA and thereby lose the virulence in host plants.

关键词： Sclerotinia sclerotiorum mycelial age oxalic acid virulence proteomics

Cloning and Characterization of an mRNA Encoding F_1-ATPase Beta-Subunit Abundant in Epithelial Cells of Mantle and Gill of Pearl Oyster, Pinctada fucata

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Tsinghua Science and Technology 2007年第4期12卷 381-388页

作者：刘亮谢莉萍熊训浩范为民陈蕾张荣庆 Institute of Marine biotechnology Department of Biological Sciences and Biotechnology Tsinghua University

In oyster biomineralization, large amounts of calcium are absorbed from external media, transported to the mineralization site, and finally deposited via a matrix-mediated process, All these activities are very energy intensive; therefore, investigations of the energy metabolism pathways of different oyster tissues will facilitate understanding of oyster biomineralization physiology. A full-length cDNA encoding the F1- ATPase beta-subunit （the F1-β-subunit, a major calalytic subunit of F-ATPase） from the pearl oyster （Pinctada fucata） was cloned using the homology strategy with a pair of degenerated primers based on the conserved regions of other animals＇ F1-β-subunit genes. Sequencing and structural analyses showed that the obtained sequence shared high identity with other animals＇ F1-β-subunits, and had a unique phosphorylation site of PKC and CK II on the external surface of the putative protein. Results from semi-quantitative reverse transcription-polymerase chain reaction and in situ hybridization demonstrated this oyster F1-β-subunit mRNA is abundant in the gill and mantle, and distributed widely in the periostracal groove, the outer folder. and the dorsal region of the mantle and in the gill epithelial cells. These tissues were the main regions that participate in biomineralization processes such as calcium uptake, transport, and matrix secretion. The results indicate that tissues involved in biomineralization have stronger energy metabolic processes and that F1-ATPase might play an important role in oyster biomineralization by providing energy transport.

关键词： biomineralization F1-β-subunit mantle gill Pinctada fucata