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A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria

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Microsystems & Nanoengineering 2020年第1期6卷 1316-1333页

作者： Sthitodhi Ghosh Kashish Aggarwal Vinitha T.U Thinh Nguyen Jungyoup Han Chong H.Ahn Department of Electrical Engineering and Computer Science Microsystems and BioMEMS LaboratoryUniversity of CincinnatiCincinnatiOH 45221USA mico biomed usa inc. 10999 Reed Hartman HighwaySTE 309CCincinnatiOH 45242USA

There has been a considerable development in microfluidic based immunodiagnostics over the past few years which has greatly favored the growth of novel point-of-care-testing(POCT).However,the realization of an inexpensive,low-power POCT needs cheap and disposable microfluidic devices that can perform autonomously with minimum user *** work,for the first time,reports the development of a new microchannel capillary flow assay(MCFA)platform that can perform chemiluminescence based ELISA with lyophilized chemiluminescent *** new MCFA platform exploits the ultra-high sensitivity of chemiluminescent detection while eliminating the shortcomings associated with liquid reagent handling,control of assay sequence and user *** functionally designed microchannels along with adequate hydrophilicity produce a sequential flow of assay reagents and autonomously performs the ultra-high sensitive chemiluminescence based ELISA for the detection of malaria biomarker such as *** MCFA platform with no external flow control and simple chemiluminescence detection can easily communicate with smartphone via USB-OTG port using a custom-designed optical *** use of the smartphone for display,data transfer,storage and analysis,as well as the source of power allows the development of a smartphone based POCT analyzer for disease *** paper reports a limit of detection(LOD)of 8 ng/mL by the smartphone analyzer which is sensitive enough to detect active malarial *** MCFA platform developed with the smartphone analyzer can be easily customized for different biomarkers,so a hand-held POCT for various infectious diseases can be envisaged with full networking capability at low cost.

关键词： POCT smart capillary

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Tissue and host tropism of influenza viruses:Importance of quantitative analysis

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Science China(Life Sciences) 2009年第12期52卷 1101-1110页

作者： ZHANG Hong1,2 1 Z-biomed, inc., Rockville, MD 20855, usa 2 Department of Respiratory Medicine, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China Z-biomed Inc. Rockville USA Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi China

It is generally accepted that human influenza viruses preferentially bind to cell-surface glycoproteins/ glycolipids containing sialic acids in α2,6-linkage; while avian and equine influenza viruses preferentially bind to those containing sialic acids in α2,3-linkage. Even though this generalized view is accurate for H3 subtype isolates, it may not be accurate and absolute for all subtypes of influenza A viruses and, therefore, needs to be reevaluated carefully and realistically. Some of the studies published in major scientific journals on the subject of tissue tropism of influenza viruses are inc.nsistent and caused confusion in the scientific community. One of the reasons for the inc.nsistency is that most studies were quantitative descriptions of sialic acid receptor distributions based on lectin or influenza virus immunohistochemistry results with limited numbers of stained cells. In addition, recent studies indicate that α2,3- and α2,6-linked sialic acids are not the sole receptors determining tissue and host tropism of influenza viruses. In fact, determinants for tissue and host tropism of human, avian and animal influenza viruses are more complex than what has been generally accepted. Other factors, such as glycan topology, concentration of invading viruses, local density of receptors, lipid raft microdomains, coreceptors or sialic acid-independent receptors, may also be important. To more efficiently control the global spread of pandemic influenza such as the current circulating influenza A H1N1, it is crucial to clarify the determinants for tissue and host tropism of influenza viruses through quantitative analysis of experimental results. In this review, I will comment on some conflicting issues related to tissue and host tropism of influenza viruses, discuss the importance of quantitative analysis of lectin and influenza virus immunohistochemistry results and point out directions for future studies in this area, which should lead to a better understanding

关键词： tissue and host tropism influenza viruses sialic acid receptors quantitative analysis

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Qualitative and quantitative analyses of influenza virus receptors in trachea and lung tissues of humans,mice,chickens and ducks

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Science China(Life Sciences) 2012年第7期55卷 612-617页

作者： WANG BaoLin LIU Bo CHEN Ling ZHANG JianYong HE HongXuan ZHANG Hong Laboratory of Respiratory Medicine Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi 563003 China National Research Center for Wildlife-borne Diseases Institute of Zoology Chinese Academy af Sciences Beijing 100101 China Z-biomed Inc. Rockville MD 20855 USA

To accurately determine the expression and distribution patterns of two infuenza virus receptors （SAa2,3-gal and SAa2,6-gal） in trachea and lung tissues of humans, mice, chickens and ducks, we analyzed lectin immunofluorescence stainings of various tissue sections qualitatively and quantitatively. Results from the qualitative analysis showed that both influenza virus receptors were expressed in lung tissues of humans, mice, chickens and ducks as well as trachea tissues of mice and ducks. However, SAa2,6-gal receptor was expressed only in the human trachea tissue and SAa2,3-gal receptor was expressed only in the chicken trachea tissue. Results from the quantitative analysis demonstrated that both receptors were expressed in trachea tis- sues of human and mouse, as well as in lung tissues of humans, chickens and ducks. Meanwhile, our results also showed that the expression and distribution of influenza virus receptors in the same tissue were not always uniform, indicating that their distribution and expression in various tissues are not simply the distinc.ion between the presence or absence of receptors, but rather the difference in the amount of expressed receptors.

关键词： influenza virus siaUc acid receptors trachea lung lectin immunofluorescence quantitative analysis qualitative analysis

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Upregulation of GLE1 and LCP2 Genes in H5N1 Influenza Virus Infected Patients

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Advances in Infectious Diseases 2016年第3期6卷 138-144页

作者： Wenfeng Peng Yaping Shi Baolin Wang Bo Liu Zhiyuan Peng Jianyong Zhang Ling Chen Hong Zhang Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi China Z-biomed Inc. Rockville USA

Previous study showed that the Gle1 RNA export mediator-like (Gle1l) gene and the lymphocyte cytosolic protein 2 (Lcp2) gene were upregulated in response to influenza virus A/Puerto Rico/8/1934 (H1N1) in a mouse mode. To determine whether these two genes were upregulated in humans after influenza A virus infection, nasopharyngeal swabs were collected from eleven patients with flu-like symptoms for viral RNA extraction and PCR amplification. Sequencing analysis revealed that nucleoprotein (NP) gene fragments amplified from nasopharyngeal swabs of four patients shared the highest similarity with the NP gene from avian influenza A (H5N1) virus (A/ goose/Shantou/753/2002). Peripheral blood samples were then collected from four patients for quantitative analysis of GLE1 and LCP2 gene expression. Our results demonstrated that both GLE1 and LCP2 genes were upregulated in H5N1 influenza A virus infected patients, suggesting that upregulation of GLE1 and LCP2 genes may be important for the host defense against influenza A viruses.

关键词： Influenza H5N1 GLE1 LCP2 Upregulation Host Defense

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美国FDA药品安全信息沟通平台和药品质量报告系统

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中国新药杂志 2014年第13期23卷 1514-1520页

作者： George R.Bolger 屠永锐孙佩华谢国伟乔卫东张泓美国Z-biomed Inc.公司洛克维尔20855 常州四药制药有限公司常州213004

美国食品药品监督管理局(FDA)的首要任务之一是及时有效地与公众、医学界和医疗卫生领域专业人员共享有关药品安全方面的信息。为了防止类似万络(Vioxx)灾难事件的重演,确保FDA监管的药品能得到安全有效使用以期达到最佳的疗效,美国FDA... 详细信息

美国食品药品监督管理局(FDA)的首要任务之一是及时有效地与公众、医学界和医疗卫生领域专业人员共享有关药品安全方面的信息。为了防止类似万络(Vioxx)灾难事件的重演,确保FDA监管的药品能得到安全有效使用以期达到最佳的疗效,美国FDA在过去的10年中已经起草、制定和修改了一系列相关的法律法规和倡议。基于这些法律和法规,美国FDA建立和强化了一个以互联网多媒体为基础,多方整合而成的药品安全信息沟通和共享平台,其中包括药品安全通讯、药品安全播客、MedWatch免费通报电话及网页、FDA药品信息脸书和推特网页及YouTube视频网页、药品安全标示变更、药品召回和药品质量报告系统。本文简明扼要地介绍目前美国FDA的药品安全信息沟通和共享平台以及药品质量报告系统。

关键词：药品安全信息共享和沟通播客平台药品质量报告系统

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美国FDA对非处方药(OTC)的监管

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中国新药杂志 2013年第24期22卷 2874-2877页

作者： Robert A.Eshelman 屠永锐严益民过星张泓美国Z-biomed Inc.公司洛克维尔20855 常州四药制药有限公司常州213004

1972年之前美国市场上大多数的非处方(OTC)药并没有按照《联邦食品、药品与化妆品法案》(FD&C)中对新药申请程序的要求通过美国食品药品监督管理局(FDA)的审批。从1972年起,FDA开始对这些非处方药进行全面审评,以建立一些条款规范这类... 详细信息

1972年之前美国市场上大多数的非处方(OTC)药并没有按照《联邦食品、药品与化妆品法案》(FD&C)中对新药申请程序的要求通过美国食品药品监督管理局(FDA)的审批。从1972年起,FDA开始对这些非处方药进行全面审评,以建立一些条款规范这类成品药品,确保其按照预期用途被认为是安全和有效的。根据这次审评制定的最终法规阐述了非处方药可以不用通过新药的审批而合法上市的特定条件。符合这个最终法规的非处方药同样还需要符合其他适用的法律法规,包括符合标签要求,CGMP,以及药品注册和登记规定。

关键词：非处方(OTC)药物非处方药审评安全和功效标示错误

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甲型流感病毒编码蛋白的突变及其致病机制

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病毒学报 2013年第4期29卷 442-451页

作者：刘波陈玲张泓贵州省遵义医学院附属医院呼吸二科呼吸医学研究室遵义563003 美国Z-biomed inc.Rockville MD 20855USA

甲型流感病毒编码的11个病毒蛋白在病毒识别宿主受体、跨种传播、病毒复制、致病性和诱导宿主免疫应答等方面分别起到不同的作用。了解甲型流感病毒编码蛋白的突变及其致病机制可为开发针对高致病性流感的通用疫苗和有效药物提供新的靶... 详细信息

甲型流感病毒编码的11个病毒蛋白在病毒识别宿主受体、跨种传播、病毒复制、致病性和诱导宿主免疫应答等方面分别起到不同的作用。了解甲型流感病毒编码蛋白的突变及其致病机制可为开发针对高致病性流感的通用疫苗和有效药物提供新的靶点。本文将主要根据近期发表的文献,综合分析有关甲型流感病毒编码蛋白关键功能位点氨基酸的变异及其与致病性和传播能力之间关系的研究进展。

关键词：甲型流感病毒基因突变致病性

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流感病毒唾液酸受体在人、小鼠、鸡及鸭气管和肺组织分布的定性与定量分析

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中国科学：生命科学 2012年第5期42卷 383-389页

作者：王宝林刘波陈玲张建勇何宏轩张泓贵州省遵义医学院附属医院呼吸内科呼吸医学研究室遵义563003 中国科学院动物研究所国家野生动物疫病研究中心北京100101 Z-biomed Inc.RockvilleMD 20855USA

为了准确地检测流感病毒唾液酸α2,3-半乳糖和唾液酸α2,6-半乳糖两种受体在人、小鼠、鸡及鸭气管和肺组织中分布和表达的特征,本研究利用凝集素免疫荧光组织染色方法对各种组织切片进行了系统的定性和定量分析.定性分析的结果,显示两... 详细信息

为了准确地检测流感病毒唾液酸α2,3-半乳糖和唾液酸α2,6-半乳糖两种受体在人、小鼠、鸡及鸭气管和肺组织中分布和表达的特征,本研究利用凝集素免疫荧光组织染色方法对各种组织切片进行了系统的定性和定量分析.定性分析的结果,显示两种流感病毒唾液酸受体在人、小鼠、鸡及鸭肺组织和小鼠及鸭气管组织中均有表达,但是,唾液酸α2,6-半乳糖受体只在人气管组织中有表达而唾液酸α2,3-半乳糖受体只在鸡气管组织中有表达.定量分析的结果显示,两种流感病毒唾液酸受体在人和小鼠的气管组织以及人、鸡和鸭的肺组织均有表达.同时,本研究结果还显示了流感病毒受体在同一组织中的分布和表达并不是均匀的,表明它们在各种组织中的分布和表达并非简单的有和无之间的区别,而是一种在表达数量上多和少的差别.

关键词：流感病毒唾液酸受体气管肺凝集素免疫荧光定量分析定性分析

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Low Doses of Rifampicin Used in New Tuberculosis Patients Correlated to inc.eased Frequency of Rifampicin-Resistance and Poorer Treatment Outcomes

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Open Journal of Medical Microbiology 2015年第2期5卷 76-84页

作者： Ling Chen Jian Du Liang Li Qi Li Qiu Zhong Yanyong Fu Bo Li Minggui Lin Liping Ma Youlun Li Xiaomeng Wang Yan Ma Xiaoying Jiang Xiaoyou Chen Qiping Ge Li Xie Xiqin Han Zhaogang Sun Guanglu Jiang Hong Zhang Weiwei Gao Affiliated Hospital of Zunyi Medical College Zunyi China Beijing Chest Hospital/Beijing Institute of Tuberculosis and Thoracic Tumor Beijing China Guangdong Center for Tuberculosis Control Guangzhou China Tianjin Center for Tuberculosis Control Tianjin China Beijing Institute for Tuberculosis Control Beijing China 309th Hospital of PLA Beijing China Henan Center for Disease Control Zhengzhou China First Affiliated Hospital Chongqing Medical University Chongqing China Zhejiang Center for Disease Control Hangzhou China Z-biomed Inc. Rockville MD USA

The prognosis of patients with previously treated tuberculosis (TB) was suggested to be dependent on whether the initial treatment was in compliance with the established guidelines. The aim of this retrospective multicenter study was to determine the proportion of new TB patients who received standard doses of rifampicin in multiple provinc.s of China, and the relationship between low doses of rifampicin and frequency of rifampicin-resistance as well as treatment outcomes. A total of 713 new TB patients were treated with either once-daily dose of bulk anti-TB drugs (group I) or every other day combination blister packs of anti-TB drugs containing rifampicin (group II) at more than 30 TB treatment centers/hospitals in China. Treatment history, therapeutic doses of rifampicin, and information about patients were extracted from their medical records and analyzed, and rifampicin-resistance of isolates collected from patients following the treatment as well as treatment outcomes were compared between two treatment groups. Among 522 patients in treatment group I, 154 (29.5%) received standard and 363 (69.5%) received low doses of rifampicin;238 (45.6%) isolates were rifampicin-resistant, and 243 (46.6%) were successfully treated. Among 191 patients in treatment group II, 175 (91.6%) received standard and 15 (7.9%) received low doses of rifampicin;72 (37.7%) isolates were rifampicin-resistant, and 105 (55%) were successfully treated. When patients who received low doses of rifampicin were compared to others within the same treatment group, inc.eased rates for rifampicin-resistance and treatment failure were observed. Results from this study showed that most new TB patients in treatment group I (69.5%) received low doses of rifampicin, and their treatment outcomes were worse than those in treatment group II, indicating that low doses of rifampicin used for the initial treatment of new TB patients were correlated to inc.eased frequency of rifampicin-resistance and poorer tre

关键词： Mycobacterium tuberculosis Rifampicin Therapeutic Doses Drug Resistance Treatment Outcomes

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构建甲型流感病毒核蛋白融合基因三种方法的比较研究

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中华实验和临床病毒学杂志 2012年第1期26卷 70-74页

作者：刘波王宝林陈玲闫静张建勇何宏轩张泓贵州省遵义医学院附属医院呼吸内科呼吸医学研究室遵义563003 Z-biomed IncRockvilleMD20855USA 中国科学院动物研究所

目的比较构建甲型流感病毒核蛋白NP融合基因的三种方法，以获取成功构建含绿色荧光蛋白融合基因的方法。方法PCR扩增甲型流感病毒核蛋白NP基因片段，运用三种不同的方法构建NP表达绿色荧光蛋白的融合基因：①含限制性内切酶酶切位点的N... 详细信息

目的比较构建甲型流感病毒核蛋白NP融合基因的三种方法，以获取成功构建含绿色荧光蛋白融合基因的方法。方法PCR扩增甲型流感病毒核蛋白NP基因片段，运用三种不同的方法构建NP表达绿色荧光蛋白的融合基因：①含限制性内切酶酶切位点的NP基因片段与pEGFP-N1克隆构建融合基因；②NP基因片段与pMD19-TVector连接、TA克隆后获得限制性内切酶酶切位点，经pEGFP—N1克隆构建融合基因；③含限制性内切酶酶切位点的NP基因片段与pMD19-TSimpleVector连接、TA克隆后经pEGFP—N1克隆构建融合基因。结果含限制性内切酶酶切位点的NP基因片段TA克隆后经pEGFP—N1克隆，获得高效且稳定的绿色荧光核蛋白融合基因，第一、第二两种方法构建绿色荧光核蛋白融合基因成功率低。结论含限制性内切酶酶切位点的NP基因片段经TA克隆与pEGFP-N1连接克隆，成功构建了绿色荧光蛋白的甲型流感病毒核蛋白NP融合基因pEGFP-N1-NP，该方法高效且重复性好。该研究为进一步了解NP蛋白的生物功能及甲型流感病毒的致病机理奠定了基础。

关键词：流感病毒A型核蛋白质类绿色荧光蛋白基因

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